25 results on '"Asmaa Tazi"'
Search Results
2. Molecular epidemiology of invasive and non-invasive group B Streptococcus circulating in Serbia
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Lazar Ranin, Philippe Glaser, Claire Poyart, Ina Gajic, Céline Plainvert, Asmaa Tazi, Dusan Kekic, Nicolas Dmytruk, Vera Mijac, Natasa Opavski, Institute of Microbiology and Immunology [Belgrade, Serbia] (School of Medicine), University of Belgrade [Belgrade], National Reference Laboratory for Streptococci, Medical Faculty, University of Belgrade, Service de Bactériologie [CHU Cochin, AP-HP], Assistance publique - Hôpitaux de Paris (AP-HP) (AP-HP)-Hôpital Cochin [AP-HP], Assistance publique - Hôpitaux de Paris (AP-HP) (AP-HP), Centre national de Référence des Streptocoques (CNR), DHU Risques Et Grossesse, Centre National de la Recherche Scientifique (CNRS)-Assistance publique - Hôpitaux de Paris (AP-HP) (AP-HP)-Université Paris Descartes - Paris 5 (UPD5)-Institut National de la Santé et de la Recherche Médicale (INSERM), Université Paris Descartes - Paris 5 (UPD5), Barrières et Pathogènes, [Institut Cochin] Departement Infection, immunité, inflammation, Institut Cochin (IC UM3 (UMR 8104 / U1016)), Université Paris Descartes - Paris 5 (UPD5)-Institut National de la Santé et de la Recherche Médicale (INSERM)-Centre National de la Recherche Scientifique (CNRS)-Université Paris Descartes - Paris 5 (UPD5)-Institut National de la Santé et de la Recherche Médicale (INSERM)-Centre National de la Recherche Scientifique (CNRS)-Institut Cochin (IC UM3 (UMR 8104 / U1016)), Université Paris Descartes - Paris 5 (UPD5)-Institut National de la Santé et de la Recherche Médicale (INSERM)-Centre National de la Recherche Scientifique (CNRS)-Université Paris Descartes - Paris 5 (UPD5)-Institut National de la Santé et de la Recherche Médicale (INSERM)-Centre National de la Recherche Scientifique (CNRS), Biologie des Bactéries pathogènes à Gram-positif, Institut Pasteur [Paris]-Centre National de la Recherche Scientifique (CNRS), This study was supported by the Federation of European Microbiological Societies [FEMS-RG-2014-0025.R1] and the SerbianMinistry of Education and Science [Project No175039]., BOUYSSIE, Reine, Assistance publique - Hôpitaux de Paris (AP-HP) (AP-HP)-Université Paris Descartes - Paris 5 (UPD5)-Institut National de la Santé et de la Recherche Médicale (INSERM)-Centre National de la Recherche Scientifique (CNRS), and Institut Pasteur [Paris] (IP)-Centre National de la Recherche Scientifique (CNRS)
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MESH: Adhesins, Bacterial ,medicine.disease_cause ,Group B ,MESH: Pregnancy ,MESH: Streptococcal Infections ,Pregnancy ,Drug Resistance, Multiple, Bacterial ,Prevalence ,Clustered Regularly Interspaced Short Palindromic Repeats ,Colonization ,Capsular type ,0303 health sciences ,MESH: Clindamycin ,Streptococcus ,Clindamycin ,General Medicine ,MESH: Infant ,3. Good health ,Infectious Diseases ,CRISPR ,Female ,Serbia ,Adult ,Microbiology (medical) ,clone (Java method) ,Penicillins ,Biology ,Microbiology ,Streptococcus agalactiae ,03 medical and health sciences ,MESH: Penicillins ,Streptococcal Infections ,MESH: Bacterial Capsules ,[SDV.BBM] Life Sciences [q-bio]/Biochemistry, Molecular Biology ,medicine ,Humans ,[SDV.BBM]Life Sciences [q-bio]/Biochemistry, Molecular Biology ,Adhesins, Bacterial ,MESH: Prevalence ,Bacterial Capsules ,Hyper-virulent clone ST17 ,030304 developmental biology ,MESH: Humans ,Molecular epidemiology ,030306 microbiology ,Pregnant women ,Neonates ,Infant ,MESH: Adult ,MESH: Drug Resistance, Multiple, Bacterial ,MESH: Streptococcus agalactiae ,MESH: Serbia ,Colonisation ,MESH: Clustered Regularly Interspaced Short Palindromic Repeats ,Multilocus sequence typing ,MESH: Female - Abstract
International audience; Streptococcus agalactiae (group B Streptococcus, GBS) remains the leading cause of invasive diseases in neonates and an important cause of infections in the elderly. The aim of this study was to access the prevalence of GBS genito-rectal colonisation of pregnant women and to evaluate the genetic characteristics of invasive and non-invasive GBS isolates recovered throughout Serbia. A total of 432 GBS isolates were tested for antimicrobial susceptibility, capsular polysaccharide (CPS) types and the presence of the hvgA gene. One hundred one randomly selected isolates were further characterized by clustered regularly interspaced short palindromic repeats (CRISPRs) analysis and/or multilocus sequence typing (MLST). The prevalence of GBS colonization in pregnant women was 15%. Overall, six capsular types (Ia, Ib, II to V) were identified, the most common being III (32.2%) and V (25.2%). The hiper-virulent clone type III/ST17 was present in 43.1% and 6.3% (p
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- 2019
3. CC17 group B Streptococcus exploits integrins for neonatal meningitis development
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Romain Deshayes de Cambronne, Lionel Costa, Caroline Rambaud, Martine Cohen-Salmon, Abdelouhab Bouaboud, Asmaa Tazi, Anne-Cécile Boulay, Anne-Sophie Bourrel, Agnès Fouet, Amandine Picart, Cristina Candeias, Claire Poyart, Julie Guignot, Guillaume Bouvier, Isabelle Plu, Cyril Anjou, Corinne Albiges-Rizo, Eva Faurobert, Institut Cochin (IC UM3 (UMR 8104 / U1016)), Institut National de la Santé et de la Recherche Médicale (INSERM)-Centre National de la Recherche Scientifique (CNRS)-Université de Paris (UP), Bioinformatique structurale - Structural Bioinformatics, Institut Pasteur [Paris]-Centre National de la Recherche Scientifique (CNRS), Centre de Bioinformatique, Biostatistique et Biologie Intégrative (C3BI), Centre interdisciplinaire de recherche en biologie (CIRB), Labex MemoLife, École normale supérieure - Paris (ENS Paris), Université Paris sciences et lettres (PSL)-Université Paris sciences et lettres (PSL)-Ecole Superieure de Physique et de Chimie Industrielles de la Ville de Paris (ESPCI Paris), Université Paris sciences et lettres (PSL)-Collège de France (CdF (institution))-École normale supérieure - Paris (ENS Paris), Université Paris sciences et lettres (PSL)-Collège de France (CdF (institution))-Centre National de la Recherche Scientifique (CNRS)-Institut National de la Santé et de la Recherche Médicale (INSERM), Laboratoire de Neuropathologie Raymond Escourolle, CHU Pitié-Salpêtrière [AP-HP], Sorbonne Université (SU)-Assistance publique - Hôpitaux de Paris (AP-HP) (AP-HP)-Sorbonne Université (SU)-Assistance publique - Hôpitaux de Paris (AP-HP) (AP-HP), Hôpital Raymond Poincaré [AP-HP], Université de Versailles Saint-Quentin-en-Yvelines (UVSQ), Institute for Advanced Biosciences / Institut pour l'Avancée des Biosciences (Grenoble) (IAB), Centre Hospitalier Universitaire [Grenoble] (CHU)-Institut National de la Santé et de la Recherche Médicale (INSERM)-Etablissement français du sang - Auvergne-Rhône-Alpes (EFS)-Centre National de la Recherche Scientifique (CNRS)-Université Grenoble Alpes (UGA), Centre national de Référence des Streptocoques (CNR), Assistance publique - Hôpitaux de Paris (AP-HP) (AP-HP), This work was supported by Agence Nationale de la Recherche (ANR, grant StrepB2brain, ANR-17-CE15-0026-01). LC is a recipient of a postdoctoral fellowship from the ANR (StrepB2brain). ASB is a doctoral fellow funded by Université de Paris. RDDC was funded by FERCM., We are grateful to Pierre-Olivier Couraud (INSERM, Paris, France) for the gift of hCMEC/D3 cells, Alain Duperray for the gift of CHO-ICAM1 cells, Yoshikazu Takada for the gift of CHO-αvβ3 cells, and Glen Ulett for the gift of the GFP plasmid. We are extremely grateful to Nathalie Strazielle and Jean-François Ghersi-Egea from BIP (CRNL, Lyon, France) for choroid plexus isolation and helpful comments. We thank Matthieu Benard from the animal facility of the Institut Cochin and Marine Gaillard for their help with animal experiments and Thomas Guilbert and Fabienne Regnier for their help with brain slice clarification. We also thank the Imag’IC and HistIM core facilities of the Cochin Institute and Shaynoor Dramsi for anti-Srr1 antibody and for critical reading of the manuscript., ANR-17-CE15-0026,strepB2brain,Franchissement de la barrière hémato-méningée par le Streptocoque du groupe B hyper-virulent CC17(2017), Hôpitaux Universitaires Paris Centre (CHU Paris Centre), Université Paris sciences et lettres (PSL)-Collège de France (CdF (institution))-Institut National de la Santé et de la Recherche Médicale (INSERM)-Centre National de la Recherche Scientifique (CNRS), Université Paris sciences et lettres (PSL)-Université Paris sciences et lettres (PSL)-Collège de France (CdF (institution))-Ecole Superieure de Physique et de Chimie Industrielles de la Ville de Paris (ESPCI Paris), Université Paris sciences et lettres (PSL)-École normale supérieure - Paris (ENS Paris), Université Paris sciences et lettres (PSL)-Institut National de la Santé et de la Recherche Médicale (INSERM)-Centre National de la Recherche Scientifique (CNRS), Sorbonne Université-Assistance publique - Hôpitaux de Paris (AP-HP) (AP-HP)-Sorbonne Université-Assistance publique - Hôpitaux de Paris (AP-HP) (AP-HP)-Sorbonne Université (SU), Etablissement français du sang - Auvergne-Rhône-Alpes (EFS)-Institut National de la Santé et de la Recherche Médicale (INSERM)-Centre Hospitalier Universitaire [Grenoble] (CHU)-Centre National de la Recherche Scientifique (CNRS)-Université Grenoble Alpes (UGA), Fouet, Agnès, Franchissement de la barrière hémato-méningée par le Streptocoque du groupe B hyper-virulent CC17 - - strepB2brain2017 - ANR-17-CE15-0026 - AAPG2017 - VALID, Institut National de la Santé et de la Recherche Médicale (INSERM)-Centre National de la Recherche Scientifique (CNRS)-Université Paris Cité (UPCité), Institut Pasteur [Paris] (IP)-Centre National de la Recherche Scientifique (CNRS), École normale supérieure - Paris (ENS-PSL), Université Paris sciences et lettres (PSL)-École normale supérieure - Paris (ENS-PSL), Laboratoire de Neuropathologie Raymond Escourolle [CHU Pitié-Salpétriêre], Assistance publique - Hôpitaux de Paris (AP-HP) (AP-HP)-Sorbonne Université (SU)-Assistance publique - Hôpitaux de Paris (AP-HP) (AP-HP)-Sorbonne Université (SU), and Assistance publique - Hôpitaux de Paris (AP-HP) (AP-HP)-Sorbonne Université (SU)
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0301 basic medicine ,Group B Streptococcus ,Integrins ,[SDV]Life Sciences [q-bio] ,[SDV.NEU.NB]Life Sciences [q-bio]/Neurons and Cognition [q-bio.NC]/Neurobiology ,Clone (cell biology) ,medicine.disease_cause ,Bacterial Adhesion ,blood brain barrier (BBB) ,0302 clinical medicine ,Bacterial infections ,[SDV.MHEP.MI]Life Sciences [q-bio]/Human health and pathology/Infectious diseases ,Internalization ,reproductive and urinary physiology ,media_common ,Infectious disease ,Streptococcus ,meningitis ,General Medicine ,3. Good health ,Srr2 ,Blood-Brain Barrier ,030220 oncology & carcinogenesis ,[SDV.MHEP.MI] Life Sciences [q-bio]/Human health and pathology/Infectious diseases ,Meningitis ,Research Article ,integrin ,media_common.quotation_subject ,Integrin ,Biology ,Microbiology ,Neonatal meningitis ,Cell Line ,Meningitis, Bacterial ,Streptococcus agalactiae ,03 medical and health sciences ,[SDV.MHEP.PED] Life Sciences [q-bio]/Human health and pathology/Pediatrics ,Streptococcal Infections ,medicine ,Animals ,Humans ,Receptors, Vitronectin ,Adhesins, Bacterial ,[SDV.MHEP.PED]Life Sciences [q-bio]/Human health and pathology/Pediatrics ,[SDV.NEU.NB] Life Sciences [q-bio]/Neurons and Cognition [q-bio.NC]/Neurobiology ,medicine.disease ,Integrin alphaVbeta3 ,bacterial infections and mycoses ,[SDV.MP.BAC]Life Sciences [q-bio]/Microbiology and Parasitology/Bacteriology ,Rats ,Bacterial adhesin ,030104 developmental biology ,Animals, Newborn ,biology.protein ,neonatal susceptibility ,bacteria ,[SDV.MP.BAC] Life Sciences [q-bio]/Microbiology and Parasitology/Bacteriology ,CC17 - Abstract
We are grateful to Pierre-Olivier Couraud (INSERM, Paris, France) for the gift of hCMEC/D3 cells, Alain Duperray for the gift of CHO-ICAM1 cells, Yoshikazu Takada for the gift of CHO-αvβ3 cells, and Glen Ulett for the gift of the GFP plasmid. We are extremely grateful to Nathalie Strazielle and Jean-François Ghersi-Egea from BIP (CRNL, Lyon, France) for choroid plexus isolation and helpful comments. We thank Matthieu Benard from the animal facility of the Institut Cochin and Marine Gaillard for their help with animal experiments and Thomas Guilbert and Fabienne Regnier for their help with brain slice clarification. We also thank the Imag’IC and HistIM core facilities of the Cochin Institute and Shaynoor Dramsi for anti-Srr1 antibody and for critical reading of the manuscript.; International audience; Group B Streptococcus (GBS) is the major cause of human neonatal infections. A single clone, designated CC17-GBS, accounts for more than 80% of meningitis cases, the most severe form of the infection. However, the events allowing blood-borne GBS to penetrate the brain remain largely elusive. In this study, we identified the host transmembrane receptors α5β1 and αvβ3 integrins as the ligands of Srr2, a major CC17-GBS-specific adhesin. Two motifs located in the binding region of Srr2 were responsible for the interaction between CC17-GBS and these integrins. We demonstrated in a blood-brain-barrier cellular model that both integrins contributed to the adhesion and internalization of CC17-GBS. Strikingly, both integrins were overexpressed during the postnatal period in the brain vessels of the blood-brain barrier and blood-cerebrospinal fluid barrier and contributed to juvenile susceptibility to CC17 meningitis. Finally, blocking these integrins decreased the ability of CC17-GBS to cross into the CNS of juvenile mice in an in vivo model of meningitis. Our study demonstrated that CC17-GBS exploits integrins in order to cross the brain vessels, leading to meningitis. Importantly, it provides host molecular insights into neonate's susceptibility to CC17-GBS meningitis, thereby opening new perspectives for therapeutic and prevention strategies of GBS-elicited meningitis.
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- 2021
4. Multidrug-Resistant Hypervirulent Group B Streptococcus in Neonatal Invasive Infections, France, 2007-2019
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Claire Poyart, Caroline Joubrel-Guyot, Amandine Frigo, Gérald Touak, Céline Plainvert, Constantin Hays, Nicolas Dmytruk, and Asmaa Tazi
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Microbiology (medical) ,group B Streptococcus ,Epidemiology ,Multidrug-Resistant Hypervirulent Group B Streptococcus in Neonatal Invasive Infections, France, 2007–2019 ,030231 tropical medicine ,lcsh:Medicine ,late-onset disease ,GBS ,medicine.disease_cause ,early-onset disease ,Group B ,lcsh:Infectious and parasitic diseases ,Microbiology ,Streptococcus agalactiae ,03 medical and health sciences ,0302 clinical medicine ,Antibiotic resistance ,Drug Resistance, Multiple, Bacterial ,Streptococcal Infections ,Medicine ,Humans ,lcsh:RC109-216 ,030212 general & internal medicine ,antimicrobial resistance ,bacteria ,reproductive and urinary physiology ,neonatal infections ,biology ,business.industry ,Streptococcus ,lcsh:R ,Infant, Newborn ,Dispatch ,Late Onset Alzheimer Disease ,Early onset disease ,biology.organism_classification ,bacterial infections and mycoses ,Multiple drug resistance ,Infectious Diseases ,hypervirulent CC17 clone ,France ,business ,Bacteria - Abstract
We analyzed group B Streptococcus (GBS) neonatal invasive infections reported during 2007–2019 in France. The hypervirulent clonal complex (CC) 17 GBS was responsible for 66% (827/1,262) of cases. The role of CC17 GBS increased over time (p for trend = 0.0001), together with the emergence of a multidrug-resistant CC17 GBS sublineage.
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- 2020
5. Persistence of group B Streptococcus vaginal colonization and prevalence of hypervirulent CC-17 clone correlate with the country of birth: a prospective 3-month follow-up cohort study
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Céline, Plainvert, Olivia, Anselem, Caroline, Joubrel, Valérie, Marcou, Amiel, Falloukh, Amandine, Frigo, Fatma, Magdoud El Alaoui, Pierre-Yves, Ancel, Pierre Henri, Jarreau, Laurent, Mandelbrot, François, Goffinet, Claire, Poyart, and Asmaa, Tazi
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Adult ,Adolescent ,Vaginal Diseases ,Infant, Newborn ,Emigrants and Immigrants ,Prenatal Care ,Clone Cells ,Streptococcus agalactiae ,Cohort Studies ,Young Adult ,Pregnancy ,Streptococcal Infections ,Prevalence ,Humans ,Female ,France ,Prospective Studies ,Pregnancy Complications, Infectious - Abstract
To identify factors associated with vaginal colonization and persistence by group B Streptococcus (GBS) and by the hypervirulent neonatal CC-17 clone in late pregnancy and after delivery, a multicentre prospective observational cohort with 3-month follow-up was established in two university hospitals, Paris area, France. Pregnant women were recruited when antenatal screening for GBS vaginal colonization at 34-38 weeks of gestational age was positive. Vaginal samples were analysed by conventional culture methods at antenatal screening, delivery, and 21 and 60 days following delivery. Identification of the hypervirulent neonatal GBS CC-17 was performed. Colonization was defined as persistent when all vaginal samples were positive for GBS. A total of 754 women were included. GBS vaginal colonization was persistent in 63% of the cases (95% CI 59%-67%). Persistent colonization was more likely in women born in Sub-Saharan Africa compared with women born in France (OR = 1.88, 95% CI 1.05-3.52), and GBS CC-17 was overrepresented in women born in Sub-Saharan Africa (OR = 2.09, 95% CI 1.20-3.57). Women born in Sub-Saharan Africa are at higher risk for GBS vaginal persistence than women born in France. This observation correlates with an increased prevalence of the hypervirulent GBS CC-17 in the former group, which likely reflect variations linked to ethnicity and vaginal community-state types and might account for the increased susceptibility of black neonates to GBS infections.
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- 2020
6. Invasive group B Streptococcus infections in non-pregnant adults: a retrospective study, France, 2007-2019
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Constantin Hays, Asmaa Tazi, Franck Letourneur, Amandine Frigo, Gérald Touak, Claire Poyart, Nicolas Dmytruk, Céline Plainvert, Xavier Vuillemin, Benjamin Saintpierre, Lucie Adoux, and Mathilde Louis
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0301 basic medicine ,Microbiology (medical) ,Adult ,Male ,medicine.medical_specialty ,Adolescent ,medicine.drug_class ,030106 microbiology ,Antibiotics ,Bacteremia ,Microbial Sensitivity Tests ,medicine.disease_cause ,Serogroup ,Group B ,Meningitis, Bacterial ,Streptococcus agalactiae ,03 medical and health sciences ,Young Adult ,0302 clinical medicine ,Risk Factors ,Internal medicine ,Streptococcal Infections ,Epidemiology ,Drug Resistance, Bacterial ,medicine ,Humans ,030212 general & internal medicine ,Typing ,reproductive and urinary physiology ,Aged ,Retrospective Studies ,Aged, 80 and over ,business.industry ,Streptococcus ,Retrospective cohort study ,General Medicine ,Middle Aged ,bacterial infections and mycoses ,medicine.disease ,Anti-Bacterial Agents ,Infectious Diseases ,Female ,France ,business ,Meningitis ,Multilocus Sequence Typing - Abstract
Group B Streptococcus (GBS) (Streptococcus agalactiae) is a pathogen of growing importance in adults. The objective of this study was to describe the features of invasive infections by GBS in non-pregnant adults.GBS infections were reported to the national reference centre for streptococci. Clinical information was abstracted from questionnaires. Capsular typing, identification of the hypervirulent CC-17 clone, and antibiotic susceptibility testing were performed for all GBS isolates. Multi-locus sequence typing and assignment to clonal complexes (CCs) was performed on a representative sample of 324 isolates.In total, 1960 GBS invasive infections were analysed from 2007 to 2019. The median age at onset was 71 years old (range 18-103). The main manifestation was bacteraemia without focus (54.5%). Meningitis was more frequent in patients under 40 (26/180, 14.4% versus 78/1780, 4.4%, p 0.0001). Capsular types Ia, Ib, II, III and V accounted for 91.0% of the cases (1786/1960). CC-1, -10, -17, -19 and -23 accounted for 96.3% (312/324) of the cases. Capsular type III and CC-17 were overrepresented in meningitis (38/104, 36.5%, p 0.001 and 22/104, 21.2%, p 0.01, respectively). All isolates were susceptible to β-lactam antibiotics. Resistance to erythromycin (32.7%) and clindamycin (26.3%) remained stable, whereas decreased susceptibility to fluoroquinolones increased, reaching 2.7% in 2019 (p for trend 0.002).This work highlights the susceptibility of the elderly to GBS infections and differences in the clinical manifestations according to the patients' age and GBS type. In agreement with worldwide reports on emerging multidrug-resistant GBS, it reinforces the need for a continued surveillance of GBS epidemiology.
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- 2020
7. Perinatal hormones favor CC17 group B Streptococcus intestinal translocation through M cells and hypervirulence in neonates
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Constantin Hays, Gérald Touak, Abdelouhab Bouaboud, Agnès Fouet, Julie Guignot, Claire Poyart, Asmaa Tazi, DHU Risques Et Grossesse, Assistance publique - Hôpitaux de Paris (AP-HP) (AP-HP)-Université Paris Descartes - Paris 5 (UPD5)-Institut National de la Santé et de la Recherche Médicale (INSERM)-Centre National de la Recherche Scientifique (CNRS), Centre national de Référence des Streptocoques (CNR), Assistance publique - Hôpitaux de Paris (AP-HP) (AP-HP), Institut Cochin (IC UM3 (UMR 8104 / U1016)), Université Paris Descartes - Paris 5 (UPD5)-Institut National de la Santé et de la Recherche Médicale (INSERM)-Centre National de la Recherche Scientifique (CNRS), Signalisation et physiopathologie des cellules épithéliales, Université Paris-Sud - Paris 11 (UP11)-Institut National de la Santé et de la Recherche Médicale (INSERM), Fouet, Agnès, and Université de Paris, Institut Cochin, Institut National de la Santé et de la Recherche Médicale INSERM U1016, Centre National de la Recherche Scientifique CNRS UMR8104, F-75014 Paris.
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QH301-705.5 ,Science ,[SDV]Life Sciences [q-bio] ,[SDV.BC.IC] Life Sciences [q-bio]/Cellular Biology/Cell Behavior [q-bio.CB] ,sex hormones ,Streptococcus agalactiae ,Mice ,Streptococcal Infections ,[SDV.BC.IC]Life Sciences [q-bio]/Cellular Biology/Cell Behavior [q-bio.CB] ,neonatal infection ,Animals ,Humans ,M cells ,Biology (General) ,[SDV.MP] Life Sciences [q-bio]/Microbiology and Parasitology ,ComputingMilieux_MISCELLANEOUS ,reproductive and urinary physiology ,Cells, Cultured ,Progesterone ,Microbiology and Infectious Disease ,CC17 clone ,Estradiol ,Models, Theoretical ,bacterial infections and mycoses ,[SDV.MP.BAC]Life Sciences [q-bio]/Microbiology and Parasitology/Bacteriology ,[SDV] Life Sciences [q-bio] ,intestinal barrier ,Disease Models, Animal ,[SDV.MP]Life Sciences [q-bio]/Microbiology and Parasitology ,Animals, Newborn ,Bacterial Translocation ,Host-Pathogen Interactions ,Medicine ,Other ,[SDV.MP.BAC] Life Sciences [q-bio]/Microbiology and Parasitology/Bacteriology ,Neonatal Sepsis ,Research Article - Abstract
International audience; Group B Streptococcus (GBS) is the leading cause of invasive bacterial neonatal infections. Late-onset diseases (LOD) occur between 7 and 89 days of life and are largely due to the CC17 GBS hypervirulent clone. We studied the impact of estradiol (E2) and progesterone (P4), which impregnate the fetus during pregnancy, on GBS neonatal infection in cellular and mouse models of hormonal exposure corresponding to concentrations found at birth (E2-P4 C 0) and over 7 days old (E2-P4 C 7). Using representative GBS isolates, we show that E2-P4 C 7 concentrations specifically favor CC17 GBS meningitis following mice oral infection. CC17 GBS crosses the intestinal barrier through M cells. This process mediated by the CC17-specific surface protein Srr2 is enhanced by E2-P4 C 7 concentrations which promote M cell differentiation and CC17 GBS invasiveness. Our findings provide an explanation for CC17 GBS responsibility in LOD in link with neonatal gastrointestinal tract maturation and hormonal imprint.
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- 2019
8. Intrapartum group B Streptococcus screening in the labor ward by Xpert® GBS real-time PCR
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C. Joubrel, Claire Poyart, Céline Plainvert, Laurent Mandelbrot, Amandine Frigo, François Goffinet, Catherine Branger, M. Ballon, O. Anselem, Asmaa Tazi, and F. El Alaoui
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Adult ,0301 basic medicine ,Microbiology (medical) ,medicine.medical_specialty ,Point-of-care testing ,030106 microbiology ,Real-Time Polymerase Chain Reaction ,medicine.disease_cause ,Infant, Newborn, Diseases ,Group B ,Streptococcus agalactiae ,03 medical and health sciences ,Medical microbiology ,Pregnancy ,Streptococcal Infections ,medicine ,Humans ,Mass Screening ,Pregnancy Complications, Infectious ,Antibiotic prophylaxis ,Obstetrics and Gynecology Department, Hospital ,reproductive and urinary physiology ,Gynecology ,Streptococcus ,Obstetrics ,business.industry ,Infant, Newborn ,Group B Streptococcus Screening ,General Medicine ,Antibiotic Prophylaxis ,bacterial infections and mycoses ,medicine.disease ,Anti-Bacterial Agents ,Infectious Diseases ,Real-time polymerase chain reaction ,Point-of-Care Testing ,Vagina ,bacteria ,Female ,business - Abstract
Group B Streptococcus (GBS) is the leading cause of neonatal infections in industrialized countries. Intrapartum antibiotic prophylaxis (IAP) given to colonized parturients is a key step for the prevention of neonatal early-onset infection. We compared the performances of Xpert® GBS polymerase chain reaction (PCR) (Cepheid, Sunnyvale, CA, USA) as a point-of-care system in labor wards to standard culture for intrapartum GBS detection. Pregnant women with a GBS-positive antenatal screening were prospectively included. A vaginal double swab was collected at the time of delivery for point-of-care Xpert® GBS PCR and GBS culture. A total of 565 pregnant women were included. Valid Xpert® GBS results were obtained for 488 (86.4%) women on the first attempt. Repeat testing improved the PCR success to 516 (91.3%) women. Among the 305 women positive for GBS by culture at delivery, only 238 (78.0%) were positive by Xpert® GBS PCR, cycle thresholds being correlated to culture quantification. Among 260 women negative for GBS culture, 56 (21.5%) were positive by Xpert® GBS PCR, including 50 where IAP was initiated before vaginal sampling. Overall, among the 565 women with GBS antenatal positive culture, only 335 (59.3%) were still positive at delivery whatever the technique used, resulting in unnecessary IAP for 40% of them. This large cohort study comparing intrapartum to antepartum GBS detection provides evidence that (i) Xpert® GBS PCR might be a valuable solution for intrapartum GBS detection compared to culture-based strategies and (ii) laboratory training of non-specialized staff is mandatory to reach the performances required for point-of-care tests.
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- 2017
9. Group B streptococcus neonatal invasive infections, France 2007–2012
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Josette Raymond, P. Trieu Cuot, Gérald Touak, Nicolas Dmytruk, Philippe Bidet, Claire Poyart, Caroline Joubrel, Solen Kernéis, Asmaa Tazi, Agnès Fouet, A. Six, DHU Risques Et Grossesse, Assistance publique - Hôpitaux de Paris (AP-HP) (AP-HP)-Université Paris Descartes - Paris 5 (UPD5)-Institut National de la Santé et de la Recherche Médicale (INSERM)-Centre National de la Recherche Scientifique (CNRS), Centre national de Référence des Streptocoques (CNR), Assistance publique - Hôpitaux de Paris (AP-HP) (AP-HP), Institut Cochin (IC UM3 (UMR 8104 / U1016)), Université Paris Descartes - Paris 5 (UPD5)-Institut National de la Santé et de la Recherche Médicale (INSERM)-Centre National de la Recherche Scientifique (CNRS), AP-HP Hôpital universitaire Robert-Debré [Paris], Biologie des Bactéries pathogènes à Gram-positif, Institut Pasteur [Paris] (IP)-Centre National de la Recherche Scientifique (CNRS), This work was supported by the Fondation pour la Recherche Médicale, INSERM, CNRS, Université Paris Descartes, the Institut Pasteur, Institut de Veille Sanitaire. A. Six was a recipient of a doctoral fellowship from the Ministère de la Recherche et de l’Enseignement supérieur and the University Paris Descartes Grant: 64111310., Assistance publique - Hôpitaux de Paris (AP-HP) (APHP)-Université Paris Descartes - Paris 5 (UPD5)-Institut National de la Santé et de la Recherche Médicale (INSERM)-Centre National de la Recherche Scientifique (CNRS), Assistance publique - Hôpitaux de Paris (AP-HP) (APHP), Institut Pasteur [Paris]-Centre National de la Recherche Scientifique (CNRS), Centre National de la Recherche Scientifique (CNRS)-Assistance publique - Hôpitaux de Paris (AP-HP) (AP-HP)-Université Paris Descartes - Paris 5 (UPD5)-Institut National de la Santé et de la Recherche Médicale (INSERM), Assistance publique - Hôpitaux de Paris (AP-HP)-Université Paris Descartes - Paris 5 ( UPD5 ) -Institut National de la Santé et de la Recherche Médicale ( INSERM ) -Centre National de la Recherche Scientifique ( CNRS ), Assistance publique - Hôpitaux de Paris (AP-HP), Institut Cochin ( UM3 (UMR 8104 / U1016) ), Université Paris Descartes - Paris 5 ( UPD5 ) -Institut National de la Santé et de la Recherche Médicale ( INSERM ) -Centre National de la Recherche Scientifique ( CNRS ), Service de microbiologie, Assistance publique - Hôpitaux de Paris (AP-HP)-Hôpital Robert Debré-Université Paris Diderot - Paris 7 ( UPD7 ), and Institut Pasteur [Paris]-Centre National de la Recherche Scientifique ( CNRS )
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Male ,group B streptococcus ,Microbiology (medical) ,medicine.medical_specialty ,medicine.drug_class ,Antibiotics ,Capsular serotype ,Bacteremia ,Disease ,[ SDV.MP.BAC ] Life Sciences [q-bio]/Microbiology and Parasitology/Bacteriology ,medicine.disease_cause ,Serogroup ,Group B ,Microbiology ,Meningitis, Bacterial ,Streptococcus agalactiae ,Internal medicine ,Streptococcal Infections ,medicine ,Humans ,risk factors ,neonatal infections ,Streptococcus ,business.industry ,Incidence (epidemiology) ,Infant, Newborn ,Gestational age ,Infant ,meningitis ,General Medicine ,medicine.disease ,Survival Analysis ,[SDV.MP.BAC]Life Sciences [q-bio]/Microbiology and Parasitology/Bacteriology ,3. Good health ,Infectious Diseases ,Female ,France ,business ,Meningitis ,hypervirulent CC17 - Abstract
International audience; Streptococcus agalactiae (group B streptococcus (GBS)) is the leading cause of invasive infections among newborns in industrialized countries, with two described syndromes: early-onset disease (EOD) and late-onset disease (LOD). Since the introduction in many countries of intrapartum antibioprophylaxis (IAP), the incidence of EOD has dramatically decreased, whereas that of LOD remains unchanged. We describe the clinical and bacteriological characteristics of 438 GBS neonatal invasive infections notified to the French National Reference Centre for Streptococci in France from 2007 to 2012. Clinical data were retrieved from hospitalization reports or questionnaires. Capsular type, assignment to the hypervirulent clonal complex (CC)17 and antibiotic susceptibility profiles were determined. One hundred and seventy-four (39.7%) and 264 (60.3%) isolates were responsible for EOD, including death in utero, and LOD, respectively. EOD was associated with bacteraemia (n = 103, 61%) and LOD with meningitis (n = 145, 55%). EOD was mainly due to capsular polysaccharide (CPS) III isolates (n = 99, 57%) and CPS Ia isolates (n = 40, 23%), and CPS III isolates were responsible for 80% (n = 211) of LOD cases. CC17 accounted for 80% (n = 121) of CPS III isolates responsible for meningitis (n = 151; total cases of meningitis, 188). Bad outcome risk factors were low gestational age and low birthweight. LOD represents almost 60% of cases of neonatal GBS disease in France and other countries in which IAP has been implemented. This observation reinforces the need to develop new prevention strategies targeting CC17, which is predominant in GBS neonatal infections.
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- 2015
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10. Risk Factors for Infant Colonization by Hypervirulent CC17 Group B Streptococcus: Toward the Understanding of Late-onset Disease
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Alban Le Monnier, Najoua El Helali, Elie Azria, Emile Falloukh, Pierre Henri Jarreau, Céline Plainvert, Aurélien Seco, Catherine Branger, Olivia Anselem, François Goffinet, Morgane Ballon, Josette Raymond, Amandine Frigo, Laurent Mandelbrot, Asmaa Tazi, Claire Poyart, Valérie Marcou, Pierre-Yves Ancel, Fatma Magdoud El Alaoui, Caroline Joubrel, and Patrick Trieu-Cuot
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Microbiology (medical) ,Adult ,Male ,medicine.medical_specialty ,Concordance ,Mothers ,Breast milk ,medicine.disease_cause ,Group B ,Streptococcus agalactiae ,Feces ,Pregnancy ,Risk Factors ,Streptococcal Infections ,medicine ,Humans ,Colonization ,Longitudinal Studies ,Prospective Studies ,Mouth ,Virulence ,business.industry ,Streptococcus ,Obstetrics ,Incidence ,Infant ,Odds ratio ,Confidence interval ,Infectious Disease Transmission, Vertical ,Major Articles and Commentaries ,Infectious Diseases ,medicine.anatomical_structure ,Vagina ,Female ,France ,business - Abstract
BACKGROUND In infants, the mode of acquisition of CC17 group B Streptococcus (GBS), the hypervirulent clone responsible for late-onset disease (LOD), remains elusive. METHODS In a prospective multicenter study in France, we evaluated GBS colonization in mother-baby pairs with 2 months of follow-up between 2012 and 2015. Criteria included positivity for GBS colonization at antenatal screening or at delivery. Maternal vaginal samples and infant oral cavity and stool samples were analyzed at delivery, 21 ± 7 days (D21), and 60 ± 7 days (D60) post-delivery. RESULTS A total of 890 mother-baby pairs were analyzed. GBS colonized 7%, 21%, and 23% of the infants at birth, D21, and D60, respectively, of which 10%, 11%, and 13% were identified as CC17 GBS. Concordance between maternal and infant GBS type was 96%. At D21, the main risk factors for infant colonization by GBS were simultaneous maternal colonization of the vagina (odds ratio [OR], 4.50; 95% confidence interval [CI], 1.69-15.61) and breast milk (OR, 7.93; 95% CI, 3.81-17.14). Importantly, 38% (95% CI, 23%-56%) of infants colonized by CC17 GBS appeared colonized for the first time at D60 vs 18% (95% CI, 14%-24%; P < .049) of infants colonized by non-CC17 GBS. Multivariate analysis showed a higher risk for de novo infant colonization by CC17 at D60 than by other GBS (OR, 2.45; 95% CI, 1.02-5.88). CONCLUSIONS The high incidence of CC17 GBS in LOD is likely due to an enhanced post-delivery mother-to-infant transmission.
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- 2018
11. Infectious Cellulitis Caused by Streptococcus halichoeri
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Thomas Hubiche, Asmaa Tazi, Céline Plainvert, Claire Poyart, A. Fribourg, and Pascal Del Giudice
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0301 basic medicine ,Male ,medicine.medical_specialty ,030106 microbiology ,Treatment outcome ,Streptococcus halichoeri ,MEDLINE ,Dermatology ,03 medical and health sciences ,Streptococcal Infections ,lcsh:Dermatology ,Medicine ,Humans ,Aged, 80 and over ,business.industry ,Streptococcus ,Cellulitis ,General Medicine ,Skin Diseases, Bacterial ,lcsh:RL1-803 ,medicine.disease ,Anti-Bacterial Agents ,Treatment Outcome ,business - Published
- 2017
12. Clinical and microbiological features associated with group B Streptococcus bone and joint infections, France 2004-2014
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Philippe Morand, J.-P. Barnier, Claire Poyart, Solen Kernéis, Vincent Cattoir, F. El Sayed, J. Loubinoux, N. Desplaces, V. Vernet, Asmaa Tazi, Céline Plainvert, B. Gislain, and Nicolas Dmytruk
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0301 basic medicine ,Microbiology (medical) ,Adult ,Male ,medicine.medical_specialty ,Adolescent ,030106 microbiology ,Erythromycin ,Comorbidity ,Microbial Sensitivity Tests ,medicine.disease_cause ,History, 21st Century ,Group B ,Streptococcus agalactiae ,03 medical and health sciences ,Young Adult ,0302 clinical medicine ,Medical microbiology ,Internal medicine ,Diabetes mellitus ,Streptococcal Infections ,Drug Resistance, Bacterial ,medicine ,Humans ,030212 general & internal medicine ,Aged ,Aged, 80 and over ,Arthritis, Infectious ,Streptococcus ,business.industry ,Osteomyelitis ,Clindamycin ,General Medicine ,Middle Aged ,medicine.disease ,Surgery ,Infectious Diseases ,Female ,France ,business ,medicine.drug ,Multilocus Sequence Typing - Abstract
This study describes the clinical and microbiological features associated with group B Streptococcus (GBS) bone and joint infections (BJIs). It was a retrospective analysis of adult cases of GBS BJIs reported to the French National Reference Center for Streptococci from January 2004 to December 2014. Clinical data and GBS molecular characteristics are reported. Strains were collected from 163 patients. The most frequent comorbidities were: solid organ cancer (n = 21, 21%) and diabetes mellitus (n = 20, 20%). The main infection sites were knee (47/155 = 30%) and hip (43/155 = 27%), and occurred on orthopedic devices in 71/148 cases (48%). CPS III (n = 47, 29%), Ia (n = 26, 16%) and V (n = 40, 25%) were predominant. Resistance to erythromycin, clindamycin and tetracycline was detected in 55/163 (34%), 35/163 (21%) and 132/163 (81%) strains, respectively. The most frequent sequence types were ST-1 (n = 21, 25%), ST-17 (n = 17, 20%) and ST-23 (n = 11, 13%). The rate of resistance to erythromycin was 0% for ST-17 strains, 52% (n = 11) for ST-1 and 44% (n = 7) for ST-23 (p 0.001). GBS bone and joint infections predominantly occur in patients aged50 years and/or with comorbidities such as cancer and diabetes mellitus. CPS type distribution and MLST are very similar to that of other adult GBS invasive infections.
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- 2017
13. Human meningitis due to Streptococcus suis in Lomé, Togo: a case report
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Mounerou Salou, Koffi A.A. Balogou, Corinne Marois-Créhan, Asmaa Tazi, Claire Poyart, Mireille Prince-David, Komi Assogba, and Céline Plainvert
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Adult ,Male ,0301 basic medicine ,Serotype ,medicine.medical_specialty ,Streptococcus suis ,Swine ,030106 microbiology ,Capsular serotype ,Case Report ,Neurologic sequelae ,Serogroup ,lcsh:Infectious and parasitic diseases ,Meningitis, Bacterial ,Zoonosis ,Tinnitus ,03 medical and health sciences ,Medical microbiology ,Streptococcal Infections ,Epidemiology ,medicine ,Animals ,Humans ,lcsh:RC109-216 ,Meningitis ,Medical history ,Immunodeficiency ,biology ,business.industry ,medicine.disease ,biology.organism_classification ,Virology ,Red Meat ,030104 developmental biology ,Infectious Diseases ,Togo ,Immunology ,business - Abstract
Background Streptococcus suis is a zoonotic pathogen which represents the leading cause of meningitis in Southeast Asia and an emerging pathogen in the Western world, the main risk factor for infection being contact with pigs. In Africa, the prevalence of S. suis infections in swine and humans is largely unrecognized, with only one recent report of a limited case series. Case presentation We describe a human case of meningitis due to S. suis in a 32-year-old man living in Togo. The patient had no particular medical history and no risk factors for immunodeficiency but reported regular contact with pork products. Using specific immunological and molecular methods, we characterized the isolate as S. suis serotype 2, ST1, one the most prevalent and virulent clone worldwide. The outcome was favorable after one week of adapted antibiotic therapy but the patient was left with severe hearing disorders. Conclusions This work highlights the emergence of this pathogen in Africa and reinforces the need for accurate epidemiological and surveillance studies of S. suis infections and for educating clinicians and exposed groups in non-endemic countries.
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- 2016
14. Rapid Emergence of Resistance to Linezolid and Mutator Phenotypes in Staphylococcus aureus Isolates from an Adult Cystic Fibrosis Patient
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Gislène Collobert, Claire Poyart, Jeanne Chapron, Dominique Hubert, Asmaa Tazi, Philippe Morand, Magalie Longo, Daniel Dusser, and Gérald Touak
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Adult ,Staphylococcus aureus ,Cystic Fibrosis ,Biology ,medicine.disease_cause ,Cystic fibrosis ,Epidemiology and Surveillance ,Microbiology ,chemistry.chemical_compound ,23S ribosomal RNA ,Acetamides ,medicine ,Humans ,heterocyclic compounds ,Pharmacology (medical) ,In patient ,Oxazolidinones ,Pharmacology ,organic chemicals ,Mutator phenotype ,Linezolid ,biochemical phenomena, metabolism, and nutrition ,bacterial infections and mycoses ,medicine.disease ,Phenotype ,Virology ,Anti-Bacterial Agents ,RNA, Ribosomal, 23S ,Infectious Diseases ,chemistry ,bacteria - Abstract
Linezolid has emerged as an important therapeutic option for the treatment of Staphylococcus aureus in patients with cystic fibrosis. We report the rapid emergence, upon treatment with linezolid, of linezolid-resistant S. aureus clinical isolates through the accumulation of resistance-associated 23S rRNA mutations, together with acquisition of an altered mutator phenotype.
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- 2013
15. Rapid detection of the 'highly virulent' group B streptococcus ST-17 clone
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Josette Raymond, Claire Poyart, Hélène Réglier-Poupet, Asmaa Tazi, Marie-Cécile Lamy, Annick Billoët, Elisabeth Couvé, Patrick Trieu-Cuot, François Guérin, Frank Kunst, Shaynoor Dramsi, Philippe Glaser, Institut Cochin (IC UM3 (UMR 8104 / U1016)), Université Paris Descartes - Paris 5 (UPD5)-Institut National de la Santé et de la Recherche Médicale (INSERM)-Centre National de la Recherche Scientifique (CNRS), Biologie des Bactéries Pathogènes à Gram-positif, Institut Pasteur [Paris] (IP)-Centre National de la Recherche Scientifique (CNRS), Hôpital Cochin [AP-HP], Assistance publique - Hôpitaux de Paris (AP-HP) (AP-HP), Génomique des Microorganismes Pathogènes, Institut Pasteur [Paris]-Centre National de la Recherche Scientifique (CNRS), CHU Cochin [AP-HP], Institut Cochin ( UM3 (UMR 8104 / U1016) ), Université Paris Descartes - Paris 5 ( UPD5 ) -Institut National de la Santé et de la Recherche Médicale ( INSERM ) -Centre National de la Recherche Scientifique ( CNRS ), and Institut Pasteur [Paris]-Centre National de la Recherche Scientifique ( CNRS )
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Adult ,Serotype ,Immunology ,Clone (cell biology) ,Virulence ,[ SDV.MP.BAC ] Life Sciences [q-bio]/Microbiology and Parasitology/Bacteriology ,Biology ,medicine.disease_cause ,Polymerase Chain Reaction ,Sensitivity and Specificity ,Microbiology ,Streptococcus agalactiae ,law.invention ,03 medical and health sciences ,Pregnancy ,law ,Streptococcal Infections ,Genotype ,medicine ,Humans ,Child ,Polymerase chain reaction ,DNA Primers ,030304 developmental biology ,0303 health sciences ,030306 microbiology ,Streptococcus ,Infant, Newborn ,Genetic Variation ,[SDV.MP.BAC]Life Sciences [q-bio]/Microbiology and Parasitology/Bacteriology ,3. Good health ,Infectious Diseases ,Genes, Bacterial ,Child, Preschool ,Multilocus sequence typing ,Female - Abstract
International audience; Group B streptococcus (GBS) is a leading cause of neonatal morbidity and mortality. Multilocus sequence typing (MLST) revealed that the sequence type ST-17 defines a "highly virulent" serotype III clone strongly associated with neonatal invasive infections. Our aim was to identify a target sequence enabling rapid, simple, and specific detection of this clone by a real-time PCR assay. Conventional methods for DNA manipulation and gene analyses were used to characterize the gbs2018 gene variant specific for ST-17 clone and to design ST-17- and GBS-specific primers. Conventional and real-time PCR assays were developed to detect GBS and ST-17 clones in bacterial cultures and directly on clinical samples. One hundred and fifty-six French GBS strains from various geographical areas in France isolated between 1990 and 2005 were screened by PCR with ST-17-specific primers. Forty strains were positive, and all were validated by MLST as ST-17. A representative sampling of 49 ST-17-PCR-negative strains was confirmed by MLST as non-ST-17. Real-time PCR was further used to directly test 85 vaginal samples. Among these, 13 were GBS-positive, and one was identified as ST-17. The association between strain invasiveness and ST-17 lineage in neonates with late onset disease was highly significant: 78% (P
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- 2006
16. Comparative evaluation of Strepto B ID®chromogenic medium and Granada media for the detection of Group B streptococcus from vaginal samples of pregnant women
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François Dautezac, Asmaa Tazi, Claire Poyart, Josette Raymond, and Hélène Réglier-Poupet
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Microbiology (medical) ,medicine.disease_cause ,Sensitivity and Specificity ,Microbiology ,Group B ,Streptococcus agalactiae ,Agar plate ,Pregnancy ,medicine ,Humans ,Molecular Biology ,Bacteriological Techniques ,Chromogenic ,business.industry ,Streptococcus ,Culture Media ,Granada medium ,medicine.anatomical_structure ,Carrier State ,Vagina ,Streptococcus pyogenes ,Female ,Pregnant Women ,business - Abstract
Two types of selective media, the chromogenic medium Strepto B ID® and two non-chromogenic media Strepto B agar® and the Granada® medium, were tested and compared to blood agar plates (BAP) for screening of Group B streptococcus vaginal colonization in pregnant women. All tested media were comparable in terms of sensitivity however, their use in routine laboratories may markedly facilitate the rapid detection of GBS in vaginal samples.
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- 2008
17. The Abi-domain protein Abx1 interacts with the CovS histidine kinase to control virulence gene expression in group B Streptococcus
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Sophie Brinster, Claire Poyart, Arnaud Firon, Asmaa Tazi, Shaynoor Dramsi, Patrick Trieu-Cuot, Philippe Glaser, Elisabeth Sauvage, Douglas T. Golenbock, Violette Da Cunha, Biologie des Bactéries pathogènes à Gram-positif, Institut Pasteur [Paris] (IP)-Centre National de la Recherche Scientifique (CNRS), Centre national de Référence des Streptocoques (CNR), Assistance publique - Hôpitaux de Paris (AP-HP) (AP-HP), Institut Cochin (IC UM3 (UMR 8104 / U1016)), Université Paris Descartes - Paris 5 (UPD5)-Institut National de la Santé et de la Recherche Médicale (INSERM)-Centre National de la Recherche Scientifique (CNRS), Génétique des génomes - Genetics of Genomes (UMR 3525), Division of Infectious Diseases and Immunology, Department of Medicine, University of Massachusetts Medical School [Worcester] (UMASS), University of Massachusetts System (UMASS)-University of Massachusetts System (UMASS), This work was supported by grants from the National Institute of Health (NIH R01 AI052455-06A1 to DTG and PTC), the Agence Nationale de la Recherche (ANR-10-BLAN-1321 FattyBact to CP and PTC, and Grant ANR-08-StrepRespire to CP), and by financial supports from the Pasteur Institute (to PTC), CNRS (to CP, PG, and PTC), INSERM and Université Paris Descartes (to CP). SB was the recipient of a postdoctoral fellowship from the Region Ile-de-France (DIM MalInf)., ANR-10-BLAN-1321,FattyBact,Impact des acides gras de l'hôte sur l'adaptation des pathogènes à Gram positif à bas pourcentage GC(2010), ANR-06-MIME-0035,StreRespire,Métabolisme respiratoire et capture de l'hème chez le streptocoque du groupe B: impact sur le commensalisme et la virulence.(2006), Lassailly-Bondaz, Anne, BLANC - Impact des acides gras de l'hôte sur l'adaptation des pathogènes à Gram positif à bas pourcentage GC - - FattyBact2010 - ANR-10-BLAN-1321 - BLANC - VALID, Programme Microbiologie, Immunologie et Maladies Emergentes - Métabolisme respiratoire et capture de l'hème chez le streptocoque du groupe B: impact sur le commensalisme et la virulence. - - StreRespire2006 - ANR-06-MIME-0035 - MIME - VALID, Institut Pasteur [Paris]-Centre National de la Recherche Scientifique (CNRS), Centre National de la Recherche Scientifique (CNRS)-Université Paris Descartes - Paris 5 (UPD5)-Institut National de la Santé et de la Recherche Médicale (INSERM), Institut Pasteur [Paris] - Centre National de la Recherche Scientifique (CNRS), Assistance publique - Hôpitaux de Paris (AP-HP), Institut Cochin (UM3 (UMR 8104 / U1016)), Université Paris Descartes - Paris 5 (UPD5) - Institut National de la Santé et de la Recherche Médicale (INSERM) - Centre National de la Recherche Scientifique (CNRS), Génétique des génomes, ANR-10-BLAN-1321, FattyBact, Impact des acides gras de l'hôte sur l'adaptation des pathogènes à Gram positif à bas pourcentage GC(2010), and ANR-06-MIME-0035, StrepRespire, Métabolisme respiratoire et capture de l'hème chez le streptocoque du groupe B: impact sur le commensalisme et la virulence.(2006)
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Histidine Kinase ,Operon ,Gene Identification and Analysis ,MESH: Amino Acid Sequence ,Biochemistry ,MESH: Protein Structure, Tertiary ,MESH: Streptococcal Infections ,Protein Interaction Mapping ,Phosphoprotein Phosphatases ,MESH: Epistasis, Genetic ,MESH: Animals ,Biology (General) ,MESH: Bacterial Proteins ,Oligonucleotide Array Sequence Analysis ,Regulation of gene expression ,0303 health sciences ,Streptococci ,Genomics ,MESH: Hemolysis ,Functional Genomics ,Bacterial Pathogens ,3. Good health ,Medicine ,Infectious diseases ,Group B streptococcal infection ,Virulence Factors ,QH301-705.5 ,Molecular Sequence Data ,Immunology ,MESH: Sequence Alignment ,Virulence ,Microbiology ,MESH: Protein-Serine-Threonine Kinases ,Molecular Genetics ,03 medical and health sciences ,MESH: Gene Expression Profiling ,Bacterial Proteins ,Genetics ,Humans ,Amino Acid Sequence ,Biology ,Microbial Pathogens ,Molecular Biology ,MESH: Protein Kinases ,MESH: Virulence Factors ,MESH: Molecular Sequence Data ,MESH: Humans ,030306 microbiology ,MESH: Protein Interaction Mapping ,Proteins ,Epistasis, Genetic ,Pigments, Biological ,MESH: Streptococcus agalactiae ,[SDV.MP.BAC]Life Sciences [q-bio]/Microbiology and Parasitology/Bacteriology ,Regulatory Proteins ,Protein Structure, Tertiary ,Transmembrane Proteins ,Mutation ,Parasitology ,Immunologic diseases. Allergy ,Protein Kinases ,MESH: Female ,MESH: Signal Transduction ,Genetic Screens ,MESH: Virulence ,MESH: Gene Expression Regulation, Bacterial ,Female ,Signal transduction ,Research Article ,Signal Transduction ,MESH: Pigments, Biological ,MESH: Mutation ,Protein family ,MESH: Rats ,Bacterial diseases ,Protein domain ,Protein Serine-Threonine Kinases ,Hemolysis ,Models, Biological ,Streptococcus agalactiae ,Streptococcal Infections ,Virology ,MESH: Phosphoprotein Phosphatases ,Animals ,Gene Regulation ,Gene Networks ,Protein Interactions ,Gene ,030304 developmental biology ,Gene Expression Profiling ,Histidine kinase ,MESH: Models, Biological ,Gene Expression Regulation, Bacterial ,RC581-607 ,Rats ,MESH: Oligonucleotide Array Sequence Analysis ,Gene Function ,[SDV.MP.BAC] Life Sciences [q-bio]/Microbiology and Parasitology/Bacteriology ,Genome Expression Analysis ,Sequence Alignment - Abstract
Group B Streptococcus (GBS), a common commensal of the female genital tract, is the leading cause of invasive infections in neonates. Expression of major GBS virulence factors, such as the hemolysin operon cyl, is regulated directly at the transcriptional level by the CovSR two-component system. Using a random genetic approach, we identified a multi-spanning transmembrane protein, Abx1, essential for the production of the GBS hemolysin. Despite its similarity to eukaryotic CaaX proteases, the Abx1 function is not involved in a post-translational modification of the GBS hemolysin. Instead, we demonstrate that Abx1 regulates transcription of several virulence genes, including those comprising the hemolysin operon, by a CovSR-dependent mechanism. By combining genetic analyses, transcriptome profiling, and site-directed mutagenesis, we showed that Abx1 is a regulator of the histidine kinase CovS. Overexpression of Abx1 is sufficient to activate virulence gene expression through CovS, overcoming the need for an additional signal. Conversely, the absence of Abx1 has the opposite effect on virulence gene expression consistent with CovS locked in a kinase-competent state. Using a bacterial two-hybrid system, direct interaction between Abx1 and CovS was mapped specifically to CovS domains involved in signal processing. We demonstrate that the CovSR two-component system is the core of a signaling pathway integrating the regulation of CovS by Abx1 in addition to the regulation of CovR by the serine/threonine kinase Stk1. In conclusion, our study reports a regulatory function for Abx1, a member of a large protein family with a characteristic Abi-domain, which forms a signaling complex with the histidine kinase CovS in GBS., Author Summary The gram-positive Streptococcus genus includes three major human pathogens that are members of the normal microflora: Streptococcus pneumoniae (also known as the pneumococcus), Streptococcus pyogenes (Group A Streptococcus), and Streptococcus agalactiae (Group B Streptococcus). Their carriage in the population is highly dynamic and mostly asymptomatic. However, each of these species can cause a wide spectrum of diseases, from local infections to systemic and fatal infections including septicemia and meningitis. Expression of streptococcal virulence-associated genes is tightly regulated at the transcriptional level. However, the signal(s) and the precise molecular events controlling the switch from commensalism to virulence are not yet understood. In this study, we identified and characterized a bacterial protein essential for virulence gene expression in Group B Streptococcus, the main pathogen of neonates. We show that this transmembrane protein, named Abx1, interacts with the histidine kinase CovS to modulate the activity of the major regulator of virulence CovR. We define how a core set of four proteins, Abx1, CovS, CovR, and the serine/threonine kinase Stk1, interact to control the expression of virulence genes in S. agalactiae. We propose that Abx1-like proteins, that are widespread in bacteria, might be part of a conserved mechanism of two-component system regulation.
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- 2013
18. Group B Streptococcus surface proteins as major determinants for meningeal tropism
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Claire Poyart, Asmaa Tazi, Isabelle Tardieux, Shaynoor Dramsi, Samuel Bellais, Patrick Trieu-Cuot, Biologie des Bactéries pathogènes à Gram-positif, Institut Pasteur [Paris] (IP)-Centre National de la Recherche Scientifique (CNRS), Service de Bactériologie, Assistance publique - Hôpitaux de Paris (AP-HP) (AP-HP)-Hôpital Cochin [AP-HP], Assistance publique - Hôpitaux de Paris (AP-HP) (AP-HP), Centre national de Référence des Streptocoques (CNR), Institut Cochin (IC UM3 (UMR 8104 / U1016)), Université Paris Descartes - Paris 5 (UPD5)-Institut National de la Santé et de la Recherche Médicale (INSERM)-Centre National de la Recherche Scientifique (CNRS), This work was supported by the Agence Nationale de la Recherche (ANR HyperVirGBS Project, ANR-08-MIE-015), INSERM, Fondation pour la Recherche Médicale (FRM), Université Paris Descartes, S.B. was a recipient of a post-doctoral fellowship from the ANR-08-MIE-015 grant and from the FRM., ANR-08-MIEN-0015,HYPERVIRGBS,Rôle de la protéine Gbs2018C dans l'hypervirulence du clone ST-17 du Streptocoque du groupe B(2008), Institut Pasteur [Paris]-Centre National de la Recherche Scientifique (CNRS), Institut Pasteur [Paris]-Centre National de la Recherche Scientifique ( CNRS ), Assistance publique - Hôpitaux de Paris (AP-HP)-CHU Cochin [AP-HP], Assistance publique - Hôpitaux de Paris (AP-HP), Institut Cochin ( UM3 (UMR 8104 / U1016) ), Université Paris Descartes - Paris 5 ( UPD5 ) -Institut National de la Santé et de la Recherche Médicale ( INSERM ) -Centre National de la Recherche Scientifique ( CNRS ), ANR-08-MIEN-0015,HYPERVIRGBS,Rôle de la protéine Gbs2018C dans l'hypervirulence du clone ST-17 du Streptocoque du groupe B ( 2008 ), Assistance publique - Hôpitaux de Paris (AP-HP) (APHP)-CHU Cochin [AP-HP], and Assistance publique - Hôpitaux de Paris (AP-HP) (APHP)
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Microbiology (medical) ,Virulence Factors ,Clone (cell biology) ,Biology ,[ SDV.MP.BAC ] Life Sciences [q-bio]/Microbiology and Parasitology/Bacteriology ,medicine.disease_cause ,Streptococcus agalactiae/growth & development ,Microbiology ,Tropism ,Group B ,Neonatal meningitis ,Streptococcus agalactiae ,03 medical and health sciences ,Meninges ,Blood-Brain Barrier/microbiology ,medicine ,Humans ,Membrane Proteins/metabolism ,030304 developmental biology ,0303 health sciences ,030306 microbiology ,Streptococcus ,Membrane Proteins ,INFECTIOUS PROCESS ,medicine.disease ,Virology ,Streptococcus agalactiae/pathogenicity ,[SDV.MP.BAC]Life Sciences [q-bio]/Microbiology and Parasitology/Bacteriology ,Virulence Factors/metabolism ,3. Good health ,Infectious Diseases ,Blood-Brain Barrier ,Meninges/microbiology - Abstract
International audience; Streptococcus agalactiae (group B Streptococcus, GBS), a normal constituent of the intestinal microbiota is the major cause of human neonatal infections and a worldwide spread 'hypervirulent' clone, GBS ST-17, is strongly associated with neonatal meningitis. Adhesion to epithelial and endothelial cells constitutes a key step of the infectious process. Therefore GBS surface-anchored proteins are obvious potential adhesion mediators of barrier crossing and determinant of hypervirulence. This review addresses the most recent molecular insights gained from studies on GBS surface proteins proven to be involved in the crossing of the brain-blood barrier and emphasizes on the specificity of a hypervirulent clone that displays meningeal tropism.
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- 2011
19. Comparison of the Diversilab® system with multi-locus sequence typing and pulsed-field gel electrophoresis for the characterization of Streptococcus agalactiae invasive strains
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Claire Poyart, Josette Raymond, Annick Billoët, Patrick Trieu-Cuot, Malik Al Nakib, Asmaa Tazi, and Magalie Longo
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Microbiology (medical) ,Adult ,Population ,Locus (genetics) ,Biology ,medicine.disease_cause ,Microbiology ,Polymerase Chain Reaction ,Streptococcus agalactiae ,Streptococcal Infections ,Genotype ,Genetic variation ,Pulsed-field gel electrophoresis ,medicine ,Humans ,Typing ,education ,Molecular Biology ,Repetitive Sequences, Nucleic Acid ,Genetics ,education.field_of_study ,Infant ,bacterial infections and mycoses ,Bacterial Typing Techniques ,Electrophoresis, Gel, Pulsed-Field ,bacteria ,Multilocus sequence typing ,Female - Abstract
Streptococcus agalactiae (or group B streptococcus; GBS) is a leading cause of neonatal morbidity and mortality in the developed countries. Several epidemiological typing tools have been developed for GBS to investigate the association between genotype and disease and to assess genetic variations within genogroups. This study compared the semi-automated repetitive sequence-based PCR Diversilab® system (DL) with MLST and pulsed field gel electrophoresis (PFGE) for determining the relatedness of invasive GBS strains. We analysed 179 unrelated GBS strains isolated from adult (n=108) and neonatal (n=71) invasive infections. By MLST, strains were resolved into 6 clonal complexes (CCs) including 23 sequence-types (STs), and 4 unique STs, whereas DL differentiated these isolates into 12 rep-PCR clusters (rPCs) and 9 unique rep-PCR types. The discriminatory power of both methods was similar, with Simpson's diversity indexes of 71.9% and 70.6%, respectively. However, their clustering concordance was low with Wallace concordance coefficients inferior to 0.4. PFGE was performed on 31 isolates representative of the most relevant DLrPCs clustered within the 3 major MLST CCs (CC-17, CC-23 and CC-1). As already observed with MLST, the concordance of DL with PFGE was low for all three CCs (Wallace coefficient
- Published
- 2010
20. The surface protein HvgA mediates group B streptococcus hypervirulence and meningeal tropism in neonates
- Author
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Samuel Bellais, Huot Khun, Nicolas Dmytruk, Charlotte Mechler, Asmaa Tazi, Claire Poyart, Patrick Trieu-Cuot, Michel-Yves Mistou, Olivier Disson, Marc Lecuit, Shaynoor Dramsi, Abdelouhab Bouaboud, Isabelle Tardieux, Institut Cochin (IC UM3 (UMR 8104 / U1016)), Université Paris Descartes - Paris 5 (UPD5)-Institut National de la Santé et de la Recherche Médicale (INSERM)-Centre National de la Recherche Scientifique (CNRS), Centre national de Référence des Streptocoques (CNR), Assistance publique - Hôpitaux de Paris (AP-HP) (AP-HP), Microorganismes et Barrières de l'Hôte (Equipe avenir), Institut National de la Santé et de la Recherche Médicale (INSERM)-Institut Pasteur [Paris], Biologie des Bactéries Pathogènes à Gram-positif, Institut Pasteur [Paris]-Centre National de la Recherche Scientifique (CNRS), Histotechnologie et Pathologie, Institut Pasteur [Paris], Hôpital Louis Mourier - AP-HP [Colombes], Université Paris Descartes - Paris 5 (UPD5), CHU Necker - Enfants Malades [AP-HP], Service des Maladies infectieuses et tropicales [CHU Necker], Assistance publique - Hôpitaux de Paris (AP-HP) (AP-HP)-Assistance publique - Hôpitaux de Paris (AP-HP) (AP-HP), This work was supported by the Agence Nationale de la Recherche (ANR HyperVirGBS Project, ANR-08-MIE-015), Institut National de la Santé et de la Recherche Médicale, Institut Pasteur, Fondation pour la Recherche Médicale (FRM), Université Paris Descartes, the Institut de Veille Sanitaire, Programme Transversal de Recherche #190. S. Bellais was a recipient of a post-doctoral fellowship from the ANR-08-MIE-015 grant and O. Disson from FRM and Institut National de la Santé et de la Recherche Médicale., We are grateful to Alexandra Gruss for helpful discussion and critical reading of the manuscript. We thank Pierre-Olivier Couraud for providing HCMEC/D3 cells., ANR-08-MIEN-0015,HYPERVIRGBS,Rôle de la protéine Gbs2018C dans l'hypervirulence du clone ST-17 du Streptocoque du groupe B(2008), Institut Pasteur [Paris] (IP)-Institut National de la Santé et de la Recherche Médicale (INSERM), Institut Pasteur [Paris] (IP)-Centre National de la Recherche Scientifique (CNRS), Institut Pasteur [Paris] (IP), Assistance publique - Hôpitaux de Paris (AP-HP) (APHP), Equipe avenir Microorganismes et Barrières de l'Hôte, Institut Pasteur [Paris]-Institut National de la Santé et de la Recherche Médicale (INSERM), Hopital Louis Mourier - AP-HP [Colombes], and Université Paris Descartes - Paris 5 (UPD5)-CHU Necker - Enfants Malades [AP-HP]-Institut des Maladies Génétiques Imagine [Paris]
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Male ,MESH: Adhesins, Bacterial ,[SDV]Life Sciences [q-bio] ,medicine.disease_cause ,Bacterial Adhesion ,MESH: Blood-Brain Barrier ,Mice ,Meninges ,MESH: Streptococcal Infections ,[SDV.MHEP.MI]Life Sciences [q-bio]/Human health and pathology/Infectious diseases ,Immunology and Allergy ,MESH: Animals ,Intestinal Mucosa ,MESH: Organ Specificity ,0303 health sciences ,Streptococcus ,MESH: Infant, Newborn ,MESH: Infant ,3. Good health ,Intestines ,MESH: Meningitis, Bacterial ,[SDV.MP]Life Sciences [q-bio]/Microbiology and Parasitology ,Blood-Brain Barrier ,Organ Specificity ,MESH: Intestinal Mucosa ,Female ,Meningitis ,MESH: Intestines ,MESH: Bacterial Translocation ,MESH: Meninges ,Immunology ,Virulence ,Context (language use) ,Biology ,Meningitis, Bacterial ,Streptococcus agalactiae ,Microbiology ,Neonatal meningitis ,03 medical and health sciences ,Streptococcal Infections ,medicine ,Animals ,Humans ,MESH: Bacterial Adhesion ,Adhesins, Bacterial ,MESH: Mice ,Tropism ,030304 developmental biology ,MESH: Humans ,030306 microbiology ,Infant, Newborn ,Brief Definitive Report ,Infant ,medicine.disease ,MESH: Streptococcus agalactiae ,[SDV.MP.BAC]Life Sciences [q-bio]/Microbiology and Parasitology/Bacteriology ,MESH: Male ,Bacterial adhesin ,Bacterial Translocation ,MESH: HeLa Cells ,MESH: Female ,[SDV.MHEP]Life Sciences [q-bio]/Human health and pathology ,HeLa Cells - Abstract
Lethal meningitis triggered by the hypervirulent group B streptococcus clone ST-17 is mediated by a novel surface protein called HvgA., Streptococcus agalactiae (group B streptococcus; GBS) is a normal constituent of the intestinal microflora and the major cause of human neonatal meningitis. A single clone, GBS ST-17, is strongly associated with a deadly form of the infection called late-onset disease (LOD), which is characterized by meningitis in infants after the first week of life. The pathophysiology of LOD remains poorly understood, but our epidemiological and histopathological results point to an oral route of infection. Here, we identify a novel ST-17–specific surface-anchored protein that we call hypervirulent GBS adhesin (HvgA), and demonstrate that its expression is required for GBS hypervirulence. GBS strains that express HvgA adhered more efficiently to intestinal epithelial cells, choroid plexus epithelial cells, and microvascular endothelial cells that constitute the blood–brain barrier (BBB), than did strains that do not express HvgA. Heterologous expression of HvgA in nonadhesive bacteria conferred the ability to adhere to intestinal barrier and BBB-constituting cells. In orally inoculated mice, HvgA was required for intestinal colonization and translocation across the intestinal barrier and the BBB, leading to meningitis. In conclusion, HvgA is a critical virulence trait of GBS in the neonatal context and stands as a promising target for the development of novel diagnostic and antibacterial strategies.
- Published
- 2010
21. Specific Distribution within the Enterobacter cloacae Complex of Strains Isolated from Infected Orthopedic Implants▿
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Valérie Dumaine, Annick Billoët, Claire Poyart, Philippe Morand, Valérie Sivadon-Tardy, Asmaa Tazi, Luc Jeanne, Martin Rottman, Philippe Anract, Jean-Pierre Courpied, and L. Eyrolle
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Microbiology (medical) ,Adult ,DNA, Bacterial ,Male ,medicine.medical_specialty ,Prosthesis-Related Infections ,Genotype ,Sequence analysis ,Molecular Sequence Data ,Biology ,Bacterial genetics ,Microbiology ,Young Adult ,Bacterial Proteins ,Molecular genetics ,Enterobacter cloacae ,medicine ,Prevalence ,Cluster Analysis ,Humans ,Gene ,Phylogeny ,Aged ,Enterobacteriaceae Infections ,Bacteriology ,Enterobacter ,Chaperonin 60 ,Sequence Analysis, DNA ,Middle Aged ,biology.organism_classification ,Enterobacteriaceae ,Female - Abstract
Bacteria belonging to the Enterobacter genus are frequently isolated from clinical samples but are unusual causative agents of orthopedic implant infections. Twelve genetic clusters (clusters I to XII) and one sequence crowd (sequence crowd xiii ) can be distinguished within the Enterobacter cloacae nomenspecies on the basis of hsp60 sequence analysis, and until now, none of these clusters could be specifically associated with a disease. In order to investigate if specific genetic clusters would be involved in infections of orthopedic material, two series of bacterial clinical isolates identified as E. cloacae by routine phenotypic identification methods were collected either from infected orthopedic implants ( n = 21) or from randomly selected samples of diverse anatomical origins (control; n = 52). Analysis of the hsp60 gene showed that genetic clusters III, VI, and VIII were the most frequent genetic clusters detected in the control group, whereas cluster III was poorly represented among the orthopedic implant isolates ( P = 0.006). On the other hand, E. hormaechei (clusters VI and VIII), but not cluster III, is predominantly associated with infections of orthopedic implants and, more specifically, with infected material in the hip ( P = 0.019). These results support the hypothesis that, among the isolates within the E. cloacae complex, E. hormaechei and hsp60 gene sequencing-based cluster III are involved in pathogenesis in different ways and highlight the need for more accurate routine Enterobacter identification methods.
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- 2009
22. Multiplex PCR assay for rapid and accurate capsular typing of group B streptococci
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Josette Raymond, Hélène Réglier-Poupet, Claire Poyart, Patrick Trieu-Cuot, Asmaa Tazi, Nicole Tavares, and Annick Billoët
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Microbiology (medical) ,Bacterial capsule ,Time Factors ,Molecular Sequence Data ,medicine.disease_cause ,Polymerase Chain Reaction ,Sensitivity and Specificity ,Group B ,Microbiology ,law.invention ,Streptococcus agalactiae ,03 medical and health sciences ,law ,Streptococcal Infections ,Multiplex polymerase chain reaction ,medicine ,Humans ,Base sequence ,Typing ,Polymerase chain reaction ,Bacterial Capsules ,030304 developmental biology ,0303 health sciences ,biology ,Base Sequence ,030306 microbiology ,Bacteriology ,Sequence Analysis, DNA ,Streptococcaceae ,biology.organism_classification ,bacterial infections and mycoses ,Virology ,3. Good health ,Bacterial Typing Techniques - Abstract
We developed a simple, specific, and sensitive two-multiplex-PCR assay that enabled the detection of all known group B streptococcal (GBS) capsular polysaccharides. This test is well adapted for GBS capsular polysaccharide typing in large-scale epidemiological studies.
- Published
- 2007
23. Fluoroquinolone-Resistant Group B Streptococci in Acute Exacerbation of Chronic Bronchitis
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Claire Poyart, Liliane Gilly, Patrick Trieu-Cuot, Emanuelle Varon, Thomas Gueudet, and Asmaa Tazi
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Microbiology (medical) ,Male ,Chronic bronchitis ,Ofloxacin ,Epidemiology ,letter ,lcsh:Medicine ,Drug resistance ,Microbial Sensitivity Tests ,medicine.disease_cause ,fluoroquinolone ,antibiotics ,lcsh:Infectious and parasitic diseases ,Microbiology ,Streptococcus agalactiae ,bronchitis ,Levofloxacin ,Moxifloxacin ,Streptococcal Infections ,Streptococcus pneumoniae ,Drug Resistance, Bacterial ,medicine ,Humans ,lcsh:RC109-216 ,heterocyclic compounds ,Letters to the Editor ,group B streptococci ,Aged, 80 and over ,drug resistance ,business.industry ,lcsh:R ,biochemical phenomena, metabolism, and nutrition ,bacterial infections and mycoses ,Anti-Bacterial Agents ,Ciprofloxacin ,Infectious Diseases ,Sparfloxacin ,DNA Gyrase ,Acute Disease ,Chronic Disease ,Mutation ,bacteria ,France ,business ,medicine.drug ,Fluoroquinolones - Abstract
To the Editor: Fluoroquinolones (FQs) that are active against streptococcal species (e.g., levofloxacin and moxifloxacin) have been recommended by numerous national health authorities and international organizations for treating acute exacerbations of chronic bronchitis and pneumonia in adults (1). However, use of these antimicrobial drugs for treating community-acquired infections has led to an increase in FQ-resistant strains in bacteria such as Streptococcus pneumoniae. Group B streptococci (GBS, e.g., S. agalactiae) are the leading cause of invasive infections (pneumonia, septicemia, and meningitis) in neonates. GBS are also associated with bacteremia, endocarditis, and arthritis, and are responsible for deaths and illness in nonpregnant women with underlying diseases and in elderly adults (2). We describe, to our knowledge, the first GBS clinical isolate in France resistant to FQ; the isolate was from a patient treated with levofloxacin. GBS CNR0717 strain was isolated as the predominant bacterium in a culture (>107 CFU/mL) from 2 purulent sputum samples from an 80-year-old man (leukocytes >25, epithelial cells 64 mg/L, and showed increased MICs for ciprofloxacin, sparfloxacin, levofloxacin, and moxifloxacin. No reduction of FQ MICs was observed with reserpine (10 mg/L), which indicated that resistance to FQ was not caused by an active efflux pump system. Table MICs of fluoroquinolones for strains of group B streptococci (GBS), France Three major mutations have been reported for FQ resistance in streptococci at codon positions 81 in gyrA and 79 or 83 in parC (4). DNA sequence analysis of these regions showed a mutation in parC (Ser 79 → Tyr) but not in the wild-type susceptible strain (NEM316). No mutation was detected in the gyrA gene. FQ resistance in streptococci is acquired through a stepwise process and has been extensively studied in S. pneumoniae. First-step mutants conferring low-level resistance generally result from mutations in either gyrA or parC. There is also a molecule-dependent target specificity: mutations in parC are generally selected by pefloxacin, ciprofloxacin, and levofloxacin, and those in gyrA are selected by sparfloxacin, gatifloxacin, moxifloxacin, gemifloxacin, and garenoxacin (5). In second-step mutants, mutations are present in both parC and gyrA and confer resistance to the antistreptococcal FQs levofloxacin, moxifloxacin, and gatifloxacin. FQ resistance in GBS has been reported in Japan, the United States, and Spain (6–8). Up to now, all FQ-resistant GBS strains described were highly resistant because of point mutations in gyrA and parC QRDR; a parC mutation at position 79 was present in all strains. These strains were isolated from elderly adults who, in some cases, had received quinolone therapy. Low-level resistance to FQ in GBS CNR0717 was associated with a Ser 79 → Tyr mutation in parC. Therefore, although the FQ sensitivity of this strain is unknown, a first-step mutant could have been selected in vivo as our patient was treated with levofloxacin for 2 weeks. GBS is an unusual cause of acute bacterial exacerbation of chronic bronchitis compared with other respiratory pathogens such as S. pneumoniae, but pathologies associated with this bacterium are changing. Clinical microbiologists should be aware of these changes and test isolates of Streptococcus spp. for susceptibility to FQs. This report indicates that FQ resistance among streptococci is a growing concern and that levofloxacin can select in vivo parC first-step mutants that will facilitate emergence of high-level FQ-resistant GBS strains, as demonstrated in vitro for S. pneumoniae (9). Finally, although FQ treatment is recommended for high-risk groups with acute exacerbations of chronic bronchitis, these antimicrobial drugs must be reserved for situations in which there are no effective alternative drugs to treat infections caused by multidrug-resistant bacteria. For susceptible strains, β-lactams, which still constitute the first-line recommended antimicrobial drugs, should be used for treatment of these patients (10).
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- 2008
24. Invasive Group B Streptococcal Infections in Infants, France
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Claire Poyart, Patrick Trieu-Cuot, Annick Billoët, Hélène Réglier-Poupet, Asmaa Tazi, Nicolas Dmytruk, Josette Raymond, Edouard Bingen, Philippe Bidet, Centre national de Référence des Streptocoques (CNR), Assistance publique - Hôpitaux de Paris (AP-HP) (AP-HP), Hôpital Cochin [AP-HP], Institut Cochin (UMR_S567 / UMR 8104), Université Paris Descartes - Paris 5 (UPD5)-Institut National de la Santé et de la Recherche Médicale (INSERM)-Centre National de la Recherche Scientifique (CNRS), Biologie des Bactéries Pathogènes à Gram-positif, Institut Pasteur [Paris]-Centre National de la Recherche Scientifique (CNRS), Assistance publique - Hôpitaux de Paris (AP-HP), CHU Cochin [AP-HP], Institut Cochin ( UMR_S567 / UMR 8104 ), Université Paris Descartes - Paris 5 ( UPD5 ) -Institut National de la Santé et de la Recherche Médicale ( INSERM ) -Centre National de la Recherche Scientifique ( CNRS ), Institut Pasteur [Paris]-Centre National de la Recherche Scientifique ( CNRS ), Dugast, Christine, and Institut Pasteur [Paris] (IP)-Centre National de la Recherche Scientifique (CNRS)
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Microbiology (medical) ,Male ,Epidemiology ,Clone (cell biology) ,Virulence ,lcsh:Medicine ,Biology ,GBS ,medicine.disease_cause ,Group B ,Microbiology ,lcsh:Infectious and parasitic diseases ,Streptococcus agalactiae ,ST-17 ,03 medical and health sciences ,Pregnancy ,[SDV.MHEP.MI]Life Sciences [q-bio]/Human health and pathology/Infectious diseases ,Streptococcal Infections ,Drug Resistance, Bacterial ,medicine ,Humans ,lcsh:RC109-216 ,Age of Onset ,030304 developmental biology ,0303 health sciences ,Molecular Epidemiology ,Molecular epidemiology ,030306 microbiology ,lcsh:R ,Dispatch ,Infant, Newborn ,meningitis ,Infant ,medicine.disease ,3. Good health ,Bacterial Typing Techniques ,[ SDV.MHEP.MI ] Life Sciences [q-bio]/Human health and pathology/Infectious diseases ,Infectious Diseases ,invasive infections ,[SDV.MHEP.MI] Life Sciences [q-bio]/Human health and pathology/Infectious diseases ,Female ,France ,Meningitis ,STREPTOCOCCAL INFECTIONS - Abstract
International audience; Clinical features and molecular characterization of 109 group B streptococci causing neonatal invasive infections were determined over an 18-month period in France. Sixty-four percent of the strains were from late-onset infections, and 75% were capsular type III. The hypervirulent clone ST-17 was recovered in 80% of meningitis cases.
25. Les enfants présentant un retard de croissance présentent une colonisation ectopique de l'intestin grêle par des bactéries buccales, qui provoquent une malabsorption des lipides dans des modèles expérimentaux
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Vonaesch, P., Araújo, J. R., Gody, J. C., Mbecko, J. R., Sanke, H., Andrianonimiadana, L., Naharimanananirina, T., Ningatoloum, S. N., Vondo, S. S., Gondje, P. B., Rodriguez-Pozo, A., Rakotondrainipiana, M., Kandou, K. J. E., Nestoret, A., Kapel, N., Djorie, S. G., Finlay, B. B., Wegener Parfrey, L., Collard, J. M., Randremanana, R. V., Sansonetti, P. J., Afribiota Investigators, Pathogénie microbienne moléculaire, Institut Pasteur [Paris] (IP)-Institut National de la Santé et de la Recherche Médicale (INSERM), Swiss Tropical and Public Health Institute [Basel], Université de Lausanne = University of Lausanne (UNIL), University of Basel (Unibas), Centre pédiatrique de Bangui, Institut Pasteur de Bangui, Réseau International des Instituts Pasteur (RIIP), Unité de Bactériologie Expérimentale [Antananarivo, Madagascar] (IPM), Institut Pasteur de Madagascar, Réseau International des Instituts Pasteur (RIIP)-Réseau International des Instituts Pasteur (RIIP), Centre Hospitalier Universitaire Joseph Ravoahangy Andrianavalona (CHUJRA), Immunologie Translationnelle - Translational Immunology lab, Institut Pasteur [Paris] (IP)-Université Paris Cité (UPCité), CHU Pitié-Salpêtrière [AP-HP], Assistance publique - Hôpitaux de Paris (AP-HP) (AP-HP)-Sorbonne Université (SU), University of British Columbia (UBC), Collège de France (CdF (institution)), This project was funded by the Total Foundation, the Institut Pasteur, Bill and Melinda Gates Foundation Grant OPP1204689, the Fondation Petram, Nutricia Foundation Grant NRF 2016-10, and a donation from the Odyssey Re-Insurance Company. P.V. was supported by Swiss National Science Foundation Early Postdoctoral Fellowship P2EZP3_152159, Advanced Postdoctoral Fellowship P300PA_177876, and Return Grant P3P3PA_17877, a Roux-Cantarini fellowship (2016), a L’Oréal–UNESCO for Women in Science France fellowship (2017), and an Excellence Scholarship from the University of Basel (Forschungsfonds, 2019). Her group is funded through the NCCR Microbiomes, a National Centre of Competence in Research, funded by the Swiss National Science Foundation (grant number 180575). Work in the group of L.W.P. is funded by Human Frontier Science Program Grant RGY0078/2015. P.J.S. is a Howard Hughes Medical Institute Senior Foreign Scholar and CIFAR scholar in the human microbiome consortium., We thank all children and their families who participated in the Afribiota project. Further, we thank the Afribiota Consortium, the participating hospitals in Bangui and Antananarivo, the Institut Pasteur, the Institut Pasteur de Madagascar, the Institut Pasteur de Bangui, and members of the scientific advisory boards for their continuous support, and we thank the Centre de Recherche Translationelle and the Direction Internationale of the Institut Pasteur (especially Paméla Palvadeau, Jane Lynda Deuve, Cécile Artaud, Nathalie Jolly, Sophie Jarrijon, Mamy Ratsialonina, and Jean-François Damaras) for help in setting up and steering the Afribiota project. We also thank J.-M.C., Pierre-Alain Rubbo, Dieu-Merci Welekoi-Yapondo, L.A., Laurence Arowas, and Marie-Noelle Ungeheuer for managing the biobank, the members of the animal facility at the Institut Pasteur for taking care of the mice, the Centre d’Immunolgoie Humaine of the Institut Pasteur, especially Milena Hasan, Tarshana Stephen, and Esma Karkeni, for help with setting up the LUMINEX assays at their platform, Asmaa Tazi for identification of the bacteria by MALDI-TOF spectroscopy, Estelle Martineau at the Platform Spectrometries Capacités at the University of Nantes for quantification of the fermentation products, Kelsey Huus for critical reading of the manuscript, and Munir Winkel for streamlining of the R code., and Liste of Afribiota Inverstigators : Laurence Barbot-Trystram, Hôpital Pitié-Salpêtrière, Paris, France Robert Barouki, Hôpital Necker- Enfants maladies, Paris, France Alexandra Bastaraud, Institut Pasteur de Madagascar, Antananarivo, Madagascar Jean-Marc Collard, Institut Pasteur de Madagascar, Antananarivo, Madagascar Maria Doria, Institut Pasteur, Paris, France Darragh Duffy, Institut Pasteur, Paris, France B. Brett Finlay, University of British Columbia, Vancouver, Canada Serge Ghislain Djorie, Institut Pasteur de Bangui, Bangui, Central African Republic Tamara Giles-Vernick, Institut Pasteur, Paris, France Bolmbaye Privat Gondje, Complexe Pédiatrique de Bangui, Bangui, Central African Republic Jean-Chrysostome Gody, Complexe Pédiatrique de Bangui, Bangui, Central African Republic Milena Hasan, Institut Pasteur, France Francis Allen Hunald, Service de Chirurgie pédiatrique, Centre Hospitalier Universitaire Joseph Ravoahangy Andrianavalona (CHU-JRA), Antananarivo, Madagascar Nathalie Kapel, Hôpital Pitié-Salpêtrière, Paris, France Jean-Pierre Lombart, Institut Pasteur de Bangui, Bangui, Central African Republic Alexandre Manirakiza, Institut Pasteur de Bangui, Bangui, Central African Republic Synthia Nazita Nigatoloum, Complexe Pédiatrique de Bangui, Bangui, Central African Republic Laura Wegener Parfrey, University of British Columbia, Vancouver, Canada Lisette Raharimalala, Centre de Santé Materno-Infantile, Tsaralalana, Antananarivo, Madagascar Maheninasy Rakotondrainipiana, Institut Pasteur de Madagascar, Antananarivo, Madagascar Rindra Vatosoa Randremanana, Institut Pasteur de Madagascar, Antananarivo, Madagascar Harifetra Mamy Richard Randriamizao, Centre Hospitalier Universitaire Joseph Ravoahangy Andrianavalona (CHU-JRA), Antananarivo, Madagascar Frédérique Randrianirina, Institut Pasteur de Madagascar, Antananarivo, Madagascar Annick Robinson, Centre Hospitalier Universitaire Mère Enfant de Tsaralalana, Antananarivo, Madagascar Pierre-Alain Rubbo, Institut Pasteur de Bangui, Bangui, République Centrafricaine Philippe Sansonetti, Institut Pasteur, Paris, France Laura Schaeffer, Institut Pasteur, Paris, France Ionela Gouandjika-Vassilache, Institut Pasteur de Bangui, Bangui, République Centrafricaine Pascale Vonaesch, Institut Pasteur, Paris, France Sonia Sandrine Vondo, Complexe Pédiatrique de Bangui, Bangui, Central African Republic Inès Vigan-Womas, Institut Pasteur de Madagascar, Antananarivo, Madagasca
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Mouth ,Multidisciplinary ,Bacteria ,lipid malabsorption ,[SDV]Life Sciences [q-bio] ,[SDV.MHEP.HEG]Life Sciences [q-bio]/Human health and pathology/Hépatology and Gastroenterology ,Models, Theoretical ,Lipid Metabolism ,Lipids ,Gastrointestinal Microbiome ,Mice ,Cross-Sectional Studies ,Malabsorption Syndromes ,stunted child growth ,Child, Preschool ,Intestine, Small ,environmental enteric dysfunction ,low-grade inflammation ,Animals ,Humans ,Leukocyte L1 Antigen Complex ,small intestine ,Growth Disorders - Abstract
International audience; Environmental enteric dysfunction (EED) is an inflammatory syndrome postulated to contribute to stunted child growth and to be associated with intestinal dysbiosis and nutrient malabsorption. However, the small intestinal contributions to EED remain poorly understood. This study aimed to assess changes in the proximal and distal intestinal microbiota in the context of stunting and EED and to test for a causal role of these bacterial isolates in the underlying pathophysiology. We performed a cross-sectional study in two African countries recruiting roughly 1,000 children aged 2 to 5 years and assessed the microbiota in the stomach, duodenum, and feces. Upper gastrointestinal samples were obtained from stunted children and stratified according to stunting severity. Fecal samples were collected. We then investigated the role of clinical isolates in EED pathophysiology using tissue culture and animal models. We find that small intestinal bacterial overgrowth (SIBO) is extremely common (>80%) in stunted children. SIBO is frequently characterized by an overgrowth of oral bacteria, leading to increased permeability and inflammation and to replacement of classical small intestinal strains. These duodenal bacterial isolates decrease lipid absorption in both cultured enterocytes and mice, providing a mechanism by which they may exacerbate EED and stunting. Further, we find a specific fecal signature associated with the EED markers fecal calprotectin and alpha-antitrypsin. Our study shows a causal implication of ectopic colonization of oral bacterial isolated from the small intestine in nutrient malabsorption and gut leakiness in vitro. These findings have important therapeutic implications for modulating the microbiota through microbiota-targeted interventions.
- Published
- 2022
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