24 results on '"Beck Z"'
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2. Impact of the expression system on the immune responses to self-assembling protein nanoparticles (SAPNs) displaying HIV-1 V1V2 loop.
- Author
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Karch CP, Paquin-Proulx D, Eller MA, Matyas GR, Burkhard P, and Beck Z
- Subjects
- Animals, Antibodies, Monoclonal genetics, Antibodies, Monoclonal immunology, Antibodies, Neutralizing drug effects, Antibodies, Neutralizing immunology, Epitopes drug effects, Epitopes immunology, Escherichia coli genetics, Gene Products, env genetics, Gene Products, env immunology, HIV Infections immunology, HIV Infections virology, HIV-1 immunology, HIV-1 pathogenicity, Humans, Immunity immunology, Immunization, HIV Infections genetics, HIV-1 genetics, Immunity genetics, Nanoparticles chemistry
- Abstract
The V1V2 loop of the Env protein is a major target for HIV-1 vaccine development because in multiple studies antibodies to this region correlated with protection. Although SAPNs expressed in E. coli elicited anti-V1V2 antibodies, the Env protein is heavily glycosylated. In this study the technology has been adapted for expression in mammalian cells. SAPNs containing a V1V2 loop from a B-subtype transmitter/founder virus were expressed in E. coli, ExpiCHO, and Expi293 cells. Independent of the expression host, particles were well-formed. All SAPNs raised high titers of V1V2-specific antibodies, however, SAPN
E.coli induced a mainly anti-V1 response, while SAPNExpiCHO and SAPNExpi293 induced a predominantly anti-V2 response. In an ADCP assay, sera from animals immunized with the SAPNExpiCHO or SAPNExpi293 induced a significant increase in phagocytic activity. This novel way of producing SAPNs displaying glycosylated epitopes could increase the antibody titer, functional activity, and shift the immune response towards the desired pathway., (Copyright © 2020 The Authors. Published by Elsevier Inc. All rights reserved.)- Published
- 2020
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3. Impact of T h 1 CD4 Follicular Helper T Cell Skewing on Antibody Responses to an HIV-1 Vaccine in Rhesus Macaques.
- Author
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Verma A, Schmidt BA, Elizaldi SR, Nguyen NK, Walter KA, Beck Z, Trinh HV, Dinasarapu AR, Lakshmanappa YS, Rane NN, Matyas GR, Rao M, Shen X, Tomaras GD, LaBranche CC, Reimann KA, Foehl DH, Gach JS, Forthal DN, Kozlowski PA, Amara RR, and Iyer SS
- Subjects
- AIDS Vaccines immunology, Adjuvants, Immunologic pharmacology, Animals, B-Lymphocytes immunology, B-Lymphocytes pathology, Female, Germinal Center immunology, Germinal Center pathology, Humans, Lipid A analogs & derivatives, Lipid A pharmacology, Macaca mulatta, Saponins pharmacology, Th1 Cells pathology, AIDS Vaccines pharmacology, HIV Antibodies immunology, HIV-1 immunology, Immunization, Secondary, Immunoglobulin G immunology, Th1 Cells immunology
- Abstract
Generating durable humoral immunity through vaccination depends upon effective interactions of follicular helper T (T
fh ) cells with germinal center (GC) B cells. Th 1 polarization of Tfh cells is an important process shaping the success of Tfh -GC B cell interactions by influencing costimulatory and cytokine-dependent Tfh help to B cells. However, the question remains as to whether adjuvant-dependent modulation of Tfh cells enhances HIV-1 vaccine-induced antienvelope (anti-Env) antibody responses. We investigated whether an HIV-1 vaccine platform designed to increase the number of Th 1-polarized Tfh cells enhances the magnitude and quality of anti-Env antibodies. Utilizing a novel interferon-induced protein 10 (IP-10)-adjuvanted HIV-1 DNA prime followed by a monophosphoryl lipid A and QS-21 (MPLA+QS-21)-adjuvanted Env protein boost (DIP-10 PALFQ ) in macaques, we observed higher anti-Env serum IgG titers with greater cross-clade reactivity, specificity for V1V2, and effector functions than in macaques primed with DNA lacking IP-10 and boosted with MPLA-plus-alum-adjuvanted Env protein (DPALFA ) The DIP-10 PALFQ vaccine regimen elicited higher anti-Env IgG1 and lower IgG4 antibody levels in serum, showing for the first time that adjuvants can dramatically impact the IgG subclass profile in macaques. The DIP-10 PALFQ regimen also increased vaginal and rectal IgA antibodies to a greater extent. Within lymph nodes, we observed augmented GC B cell responses and the promotion of Th 1 gene expression profiles in GC Tfh cells. The frequency of GC Tfh cells correlated with both the magnitude and avidity of anti-Env serum IgG. Together, these data suggest that adjuvant-induced stimulation of Th 1-Tfh cells is an effective strategy for enhancing the magnitude and quality of anti-Env antibody responses. IMPORTANCE The results of the RV144 trial demonstrated that vaccination could prevent HIV transmission in humans and that longevity of anti-Env antibodies may be key to this protection. Efforts to improve upon the prime-boost vaccine regimen used in RV144 have indicated that booster immunizations can increase serum anti-Env antibody titers but only transiently. Poor antibody durability hampers efforts to develop an effective HIV-1 vaccine. This study was designed to identify the specific elements involved in the immunological mechanism necessary to produce robust HIV-1-specific antibodies in rhesus macaques. By clearly defining immune-mediated pathways that improve the magnitude and functionality of the anti-HIV-1 antibody response, we will have the foundation necessary for the rational development of an HIV-1 vaccine., (Copyright © 2020 Verma et al.)- Published
- 2020
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4. Self-Assembling Protein Nanoparticles: implications for HIV-1 vaccine development.
- Author
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Karch CP, Matyas GR, Burkhard P, and Beck Z
- Subjects
- AIDS Vaccines chemistry, Animals, Computer Simulation, Epitopes, HIV Antigens immunology, Humans, Protein Binding, Protein Conformation, Protein Multimerization, AIDS Vaccines immunology, HIV-1 immunology, Nanoparticles chemistry, Proteins chemistry
- Published
- 2018
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5. Platelets and erythrocyte-bound platelets bind infectious HIV-1 in plasma of chronically infected patients.
- Author
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Beck Z, Jagodzinski LL, Eller MA, Thelian D, Matyas GR, Kunz AN, and Alving CR
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- HIV Infections virology, HIV-1 pathogenicity, Host-Pathogen Interactions, Humans, Blood Platelets virology, Erythrocytes virology, HIV Infections blood, HIV-1 physiology, Membrane Fusion
- Abstract
Chronic HIV-1 infection is associated with persistent viremia in most patients, but it remains unclear how free virus may survive the potential hostile effects of plasma. We investigated whether sites might exist on the surfaces of circulating blood cells for protection of infectious HIV-1 particles. Red blood cells (RBC) either from blood of uninfected normal individuals, or from blood obtained without EDTA from chronically infected HIV-1 patients, invariably contained a small number of RBC having attached platelets as determined by flow cytometry, light microscopy, and immunofluorescence microscopy. After mixing normal RBC with platelet-rich plasma, discrete populations of RBC, platelets, and complexes of platelets attached to RBC were purified by fluorescence-activated cell sorting. Upon incubation of purified cells or platelets with HIV-1 followed by washing and co-incubation with CD4-positive peripheral blood mononuclear cells (PBMC), platelets, and platelet-RBC complexes, but not platelet-free RBC, caused infection of PBMC. Infection was prevented by pre-treating the platelet-RBC complexes with EDTA. Plasma and RBC (comprising a RBC/platelet-RBC mixture) from chronically infected patients with low viral loads were also co-incubated with PBMC ex vivo to determine the presence of infectious HIV-1. All freshly isolated plasmas from the HIV-1-infected donors, obtained in the absence of anticoagulant, were noninfectious. Interestingly, the RBC from most of the patients caused cell-cell infection of PBMC that was prevented by stripping the RBC with EDTA. A monoclonal antibody to DC-SIGN partially inhibited cell-cell HIV-1 infection of PBMC by normal RBC pre-incubated with platelets and HIV-1. We conclude: (a) platelet-free EDTA-free plasma from chronically infected HIV-1 patients, although containing viral RNA, is an environment that lacks detectable infectious HIV-1; (b) platelets and platelet-RBC complexes, but not purified RBC, bind infectious HIV-1; (c) DC-SIGN, and possibly other C-type lectins, may represent binding sites for infectious HIV-1 on platelets and platelet-RBC complexes.
- Published
- 2013
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6. Infection of human peripheral blood mononuclear cells by erythrocyte-bound HIV-1: effects of antibodies and complement.
- Author
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Beck Z, Brown BK, Matyas GR, Polonis VR, Rao M, and Alving CR
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- Antibodies, Neutralizing immunology, Antibody-Dependent Enhancement, HIV Infections immunology, HIV-1 immunology, Humans, Neutralization Tests, Virus Attachment, Complement System Proteins immunology, Erythrocytes virology, HIV Antibodies immunology, HIV Infections virology, HIV-1 pathogenicity, Leukocytes, Mononuclear virology
- Abstract
Two human monoclonal antibodies, 4E10 and b12, were examined for antibody-dependent neutralization, or antibody-dependent complement (C)-mediated neutralization, of infection of peripheral blood mononuclear cells (PBMC) by either free HIV-1 or trans infection by HIV bound to erythrocytes. Neutralization of free HIV-1 by b12 was stronger than by 4E10, but b12 neutralized erythrocyte-bound HIV-1 less efficiently than cell-free virus. 4E10 did not neutralize erythrocyte-bound HIV-1 and at a low concentration it caused enhancement of infection. Antibody (4E10)-dependent C activation inhibited trans infection by erythrocyte-bound HIV-1, but caused enhanced infection with cell-free HIV-1 in the presence of erythrocytes. No effects of C were observed with b12. C-dependent enhancement in the presence of erythrocytes is proposed as due to binding of C3b-4E10-cell-free-HIV or C3d-4E10-cell-free-HIV to C receptor type 1 (CR1) on erythrocytes, or C receptor type 2 (CR2) on B cells in the PBMC, followed by trans infection of susceptible cells., (Published by Elsevier Inc.)
- Published
- 2011
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7. New cholesterol-specific antibodies remodel HIV-1 target cells' surface and inhibit their in vitro virus production.
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Beck Z, Balogh A, Kis A, Izsépi E, Cervenak L, László G, Bíró A, Liliom K, Mocsár G, Vámosi G, Füst G, and Matko J
- Subjects
- Animals, Antibodies, Monoclonal immunology, CD4 Antigens metabolism, Cell Line, Humans, Immunoglobulin G immunology, Immunoglobulin G pharmacology, Macrophages immunology, Macrophages metabolism, Membrane Microdomains immunology, Membrane Microdomains metabolism, Mice, Movement, Receptors, CXCR4 metabolism, Surface Plasmon Resonance, T-Lymphocytes immunology, T-Lymphocytes metabolism, Virus Attachment, Virus Internalization, Antibodies, Monoclonal pharmacology, Antibody Specificity immunology, Cholesterol immunology, Cholesterol metabolism, HIV Infections virology, HIV-1 drug effects, HIV-1 physiology, Virus Replication drug effects
- Abstract
The importance of membrane rafts in HIV-1 infection is still in the focus of interest. Here, we report that new monoclonal anticholesterol IgG antibodies (ACHAs), recognizing clustered membrane cholesterol (e.g., in lipid rafts), rearrange the lateral molecular organization of HIV-1 receptors and coreceptors in the plasma membrane of HIV-1 permissive human T-cells and macrophages. This remodeling is accompanied with a substantial inhibition of their infection and HIV-1 production in vitro. ACHAs promote the association of CXCR4 with both CD4 and lipid rafts, consistent with the decreased lateral mobility of CXCR4, while Fab fragments of ACHAs do not show these effects. ACHAs do not directly mask the extracellular domains of either CD4 or CXCR4 nor do they affect CXCR4 internalization. No significant inhibition of HIV production is seen when the virus is preincubated with the antibodies prior to infection. Thus, we propose that the observed inhibition is mainly due to the membrane remodeling induced by cholesterol-specific antibodies on the target cells. This, in turn, may prevent the proper spatio-temporal juxtaposition of HIV-1 glycoproteins with CD4 and chemokine receptors, thus negatively interfering with virus attachment/entry.
- Published
- 2010
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8. Human erythrocytes selectively bind and enrich infectious HIV-1 virions.
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Beck Z, Brown BK, Wieczorek L, Peachman KK, Matyas GR, Polonis VR, Rao M, and Alving CR
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- Adsorption, CD4-Positive T-Lymphocytes metabolism, CD4-Positive T-Lymphocytes virology, Erythrocyte Membrane metabolism, Erythrocyte Membrane virology, HIV-1 isolation & purification, HIV-1 pathogenicity, Humans, Leukapheresis, Leukocytes metabolism, Leukocytes virology, Virus Internalization, Erythrocytes metabolism, Erythrocytes virology, HIV Infections virology, HIV-1 metabolism, Virion metabolism, Virus Attachment
- Abstract
Although CD4(+) cells represent the major target for HIV infection in blood, claims of complement-independent binding of HIV-1 to erythrocytes and the possible role of Duffy blood group antigen, have generated controversy. To examine the question of binding to erythrocytes, HIV-1 was incubated in vitro with erythrocytes from 30 healthy leukapheresis donors, and binding was determined by p24 analysis and adsorption of HIV-1 with reduction of infectivity for CD4(+) target cells. All of the cells, regardless of blood group type, bound HIV-1 p24. A typical preparation of erythrocytes bound <2.4% of the added p24, but erythrocytes selectively removed essentially all of the viral infectivity as determined by decreased infection of CD4(+) target cells; however, cell-associated HIV-1 was approximately 100-fold more efficient, via trans infection, than unadsorbed virus for infection of CD4(+) cells. All of the bound HIV-1 p24 was released by treatment of the cells with EDTA, and binding was optimized by adding Ca(2+) and Mg(2+) during the washing of erythrocytes containing bound HIV-1. Although the small number of contaminating leukocytes in the erythrocyte preparation also bound HIV-1 p24, there was no significant binding to CD4, and it thus appears that the binding occurred on leukocytes at non-CD4 sites. Furthermore, binding occurred to erythrocyte ghosts from which contaminating leukocytes had been previously removed. The results demonstrate that erythrocytes incubated in vitro with HIV-1 differentially adsorb all of the infectious HIV-1 virions (as opposed to non-infectious or degraded virions) in the absence of complement and independent of blood group, and binding is dependent on divalent cations. By analogy with HIV-1 bound to DC-SIGN on dendritic cells, erythrocyte-bound HIV-1 might comprise an important surface reservoir for trans infection of permissive cells.
- Published
- 2009
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9. Neutralizing antibodies induced by liposomal HIV-1 glycoprotein 41 peptide simultaneously bind to both the 2F5 or 4E10 epitope and lipid epitopes.
- Author
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Matyas GR, Wieczorek L, Beck Z, Ochsenbauer-Jambor C, Kappes JC, Michael NL, Polonis VR, and Alving CR
- Subjects
- AIDS Vaccines economics, Amino Acid Sequence, Animals, Enzyme-Linked Immunosorbent Assay, Female, Liposomes immunology, Mice, Phosphatidylinositol Phosphates immunology, AIDS Vaccines immunology, Antibodies, Monoclonal immunology, Antibodies, Neutralizing immunology, Epitopes immunology, HIV Envelope Protein gp41 immunology, HIV-1 immunology
- Abstract
Objectives: There is a need to develop HIV-1 vaccine formulations that incorporate inexpensive antigens and clinically acceptable potent adjuvants for inducing neutralizing antibodies. The purpose of this initial vaccine study was to produce peptide- and lipid-induced murine mAbs that replicate the characteristics of the 2F5 and/or 4E10 human antibodies in binding both to the membrane proximal external region (MPER) of glycoprotein 41 and the adjacent lipid bilayer for neutralizing HIV-1 infection of CD4 lymphocytes., Research Designs and Methods: Liposomes containing a synthetic MPER peptide as a peptide antigen, phosphatidylinositol-4-phosphate (PIP) as a lipid antigen, and monophosphoryl lipid A as a potent adjuvant were used as a formulation to immunize mice. mAbs were then produced and tested for binding to MPER, glycoprotein 41, and PIP and for the ability to neutralize HIV-1 infection of CD4 cells in a human peripheral blood mononuclear cell assay., Results: Polyclonal antisera contained antibodies that bound both to MPER and PIP. Immunoglobulin M mAbs were produced that bound both to the core MPER site of 2F5, or that overlapped with the 4E10 site, and that simultaneously bound PIP. High concentrations of these mAbs neutralized infection of peripheral blood lymphocytes by a primary infectious molecular clone of HIV-1., Conclusion: Liposomes containing MPER peptide as an antigen, PIP as a lipid antigen, and lipid A as an adjuvant induce anti-MPER-specific multispecific antibodies that simultaneously bind glycoprotein 41 MPER and adjacent lipid and neutralize HIV-1 infection in a human peripheral blood mononuclear cell assay.
- Published
- 2009
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10. Lipid binding properties of 4E10, 2F5, and WR304 monoclonal antibodies that neutralize HIV-1.
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Matyas GR, Beck Z, Karasavvas N, and Alving CR
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- Antibodies, Monoclonal immunology, Cholesterol chemistry, Enzyme-Linked Immunosorbent Assay, Humans, Lipid A chemistry, Phospholipids chemistry, Squalene chemistry, Antibodies, Monoclonal chemistry, HIV-1 immunology, Lipids chemistry
- Abstract
Two human mAbs (2F5 and 4E10), originally derived from HIV-1-infected patients, are important, but rare, mAbs that exhibit broad cross-clade neutralizing activities against HIV-1. In addition to peptide sequences on the gp41 envelope protein, both antibodies reportedly also bound specifically to several phospholipid antigens. However, the phospholipid binding property of 2F5 has been disputed and, because of uncertainly regarding phospholipid binding, the modeling of neutralizing mechanisms has been difficult. To explore this issue, we examined the binding of 4E10 and 2F5 to a broad range of lipid antigens by ELISA. 4E10 and 2F5 both bound to a variety of purified phospholipids, and 4E10 bound, but 2F5 did not bind, to cardiolipin. Both mAbs also bound to a sulfated glycolipid, sulfogalactosyl ceramide (sulfatide), and to two neutral glycolipids, galactosyl ceramide and glucosyl ceramide, but not to other galactosyl glycolipids. 4E10, but not 2F5, also bound to cholesterol, although both mAbs bound to squalene. Interestingly, 4E10, but not 2F5, exhibited striking binding to lipid A, the lipid moiety of Gram-negative bacterial lipopolysaccharide. The binding properties of 4E10 to phospholipids, sulfatide, cholesterol, squalene, and lipid A were similar to those of a neutralizing murine mAb (WR304) induced by liposomes containing phosphatidylinositol phosphate and lipid A, although WR304 did not bind to neutral glycolipids. The discovery of a binding specificity of 4E10 for lipid A, a widely used vaccine adjuvant, suggests that innate immunity stimulated by lipid A could have played a role for induction of multispecific antibodies that simultaneously recognize both HIV-1 protein and lipid antigens.
- Published
- 2009
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11. 4-Thio-uridylate (UD29) interferes with the function of protein -SH and inhibits HIV replication in vitro.
- Author
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Beck Z, Kis A, Berényi E, Kovács P, Fésüs L, and Aradi J
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- Animals, Humans, Mice, Mice, Inbred BALB C, Uridine Monophosphate pharmacology, Anti-HIV Agents pharmacology, Enzyme Inhibitors pharmacology, Glyceraldehyde-3-Phosphate Dehydrogenases antagonists & inhibitors, HIV-1 drug effects, Sulfhydryl Compounds metabolism, Thionucleotides pharmacology, Uridine Monophosphate analogs & derivatives, Virus Replication drug effects
- Abstract
In this short communication, it is shown that 4-thio-uridylate (s(4)UMP, designated as UD29) inhibits glyceraldehyde 3-phosphate dehydrogenase (GAPDH), suggesting that the enol-form of the thiolated nucleotide may interfere with the function of the essential -SH group in the active center of the enzyme. Since HIV entry requires thiol/disulfide exchange processes, this activity prompted us to study the anti-HIV activity of the nucleotide. Indeed, UD29 inhibited the replication of HIV-1(IIIB) in the MT-4 cell line and HIV-1(Ada-M) in peripheral blood mononuclear cells (PBMC). Furthermore, UD29 was not toxic in PBMCs in vitro or in mice when the compound was administered intravenously.
- Published
- 2009
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12. Membrane-specific antibodies induced by liposomes can simultaneously bind to HIV-1 protein, peptide, and membrane lipid epitopes.
- Author
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Beck Z, Karasavvas N, Matyas GR, and Alving CR
- Subjects
- Amino Acid Sequence, Animals, Antibodies, Monoclonal immunology, Cross Reactions, Enzyme-Linked Immunosorbent Assay, Female, HIV Envelope Protein gp41 chemistry, Mice, Mice, Inbred BALB C, Molecular Sequence Data, Peptides chemistry, Antibodies, Monoclonal biosynthesis, Antibody Formation, Binding Sites, Antibody, HIV Envelope Protein gp41 metabolism, HIV-1 metabolism, Liposomes, Membrane Lipids metabolism, Membrane Proteins biosynthesis, Peptides metabolism
- Abstract
Liposomes containing lipid A as an adjuvant and also containing either both (a) cholesterol and gp140 HIV envelope protein or (b) galactosylceramide and a 48 amino acid peptide from the membrane proximal external region of gp41 from HIV, as liposomal antigens were used for immunizing mice. Monoclonal antibodies from each type of immunization were obtained, which recognized either the lipid antigen or the protein (or peptide) antigen separately, or that simultaneously bound to both the lipid and protein (or peptide) antigens, by ELISA. After immunizing with liposomes containing both phosphatidylinositol 4-phosphate (PIP) and peptide antigen, a unique monoclonal antibody was also obtained, which did not bind separately either to the lipid or peptide antigen, or to the liposomes alone, but did bind to the original liposomal antigen containing the peptide but lacking PIP. Our data suggest that immunization with liposomes containing lipid A and also containing both a lipid and protein (or peptide) antigen induces antibodies that recognize broad topographical antigenic liposomal membrane surface patterns. These membrane-specific antibodies have unique binding characteristics.
- Published
- 2008
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13. Monoclonal antibodies to phosphatidylinositol phosphate neutralize human immunodeficiency virus type 1: role of phosphate-binding subsites.
- Author
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Brown BK, Karasavvas N, Beck Z, Matyas GR, Birx DL, Polonis VR, and Alving CR
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- Animals, Antibodies, Monoclonal metabolism, Antibody Specificity, Cells, Cultured, Cross Reactions, Humans, Inositol metabolism, Leukocytes, Mononuclear, Lipid Metabolism, Lipids chemistry, Mice, Neutralization Tests, Phosphates metabolism, Antibodies, Monoclonal immunology, HIV-1 immunology, Phosphatidylinositol Phosphates immunology
- Abstract
Both a murine monoclonal antibody to phosphatidylinositol phosphate (PIP) and a human monoclonal antibody (4E10) that is known to have broadly neutralizing capabilities against primary isolates of human immunodeficiency virus type 1 (HIV-1) bound to PIP, as determined by enzyme-linked immunosorbent assay. Each of the antibodies had antigen subsite binding specificities in aqueous medium for small phosphate-containing molecules and for inositol. The anti-PIP monoclonal antibody inhibited infection by two HIV-1 primary isolates in neutralization assays employing primary human peripheral blood mononuclear cells. The data suggest that PIP or related lipids having free phosphates could serve as targets for the neutralization of HIV-1.
- Published
- 2007
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14. HIV-1, lipid rafts, and antibodies to liposomes: implications for anti-viral-neutralizing antibodies.
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Alving CR, Beck Z, Karasavva N, Matyas GR, and Rao M
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- Amino Acid Sequence, Antibodies, Viral blood, Antibodies, Viral therapeutic use, Antigens, Viral immunology, Cholesterol immunology, HIV Envelope Protein gp41 genetics, HIV Infections immunology, HIV Infections prevention & control, HIV-1 genetics, HIV-1 metabolism, Humans, Lipid Bilayers immunology, Liposomes metabolism, Models, Biological, Molecular Sequence Data, Phospholipids immunology, Antibodies, Viral immunology, HIV-1 immunology, Liposomes immunology, Membrane Microdomains immunology
- Abstract
The human immunodeficiency virus type 1 (HIV-1) is an enveloped virus with a lipid bilayer that contains several glycoproteins that are anchored in, or closely associated with, the membrane surface. The envelope proteins have complex interactions with the lipids both on the host cells and on the target cells. The processes of budding from host cells and entry into target cells occur at sites on the plasma membrane, known as lipid rafts, that represent specialized regions that are rich in cholesterol and sphingolipids. Although the envelope glycoproteins are antigenic molecules that potentially might be used for development of broadly neutralizing antibodies in a vaccine to HIV-1, the development of such antibodies that have broad specificities against primary field isolates of virus has been largely thwarted to date by the ability of the envelope proteins to evade the immune system through various mechanisms. In this review, the interactions of HIV-1 with membrane lipids are summarized. Liposomes are commonly used as models for understanding interactions of proteins with membrane lipids; and liposomes have also been used both as carriers for vaccines, and as antigens for induction of antibodies to liposomal lipids. The possibility is proposed that liposomal lipids, or liposome-protein combinations, could be useful as antigens for inducing broadly neutralizing antibodies to HIV-1.
- Published
- 2006
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15. Human herpesvirus 6A decreases the susceptibility of macrophages to R5 variants of human immunodeficiency virus 1: possible role of RANTES and IL-8.
- Author
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Csoma E, Deli T, Kónya J, Csernoch L, Beck Z, and Gergely L
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- Cells, Cultured, Chemokine CCL5 metabolism, Humans, Interleukin-8 metabolism, Leukocytes, Mononuclear, Macrophages metabolism, Macrophages virology, Receptors, CCR5 metabolism, Virus Replication, Chemokine CCL5 physiology, HIV Infections virology, HIV-1 physiology, Herpesvirus 6, Human physiology, Interleukin-8 physiology
- Abstract
Human herpesvirus 6 (HHV-6) frequently reactivates in human immunodeficiency virus 1 (HIV-1) infected patients, and is thought to be a cofactor in AIDS progression. Macrophages are targets and reservoirs of HIV-1 and HHV-6; hence, they have an important role in dissemination and pathogenesis of these viruses. The present study examined the effects of HHV-6 A variant on replication of R5 variants of HIV-1 in macrophages. For this purpose, HIV-1 replication was investigated in macrophages infected with HIV-1 alone or along with HHV-6A. Our results demonstrated that HHV-6A significantly suppressed HIV-1 replication in coinfected cultures. HHV-6A infection resulted in increased secretion of RANTES and IL-8. Experiments with exogenous RANTES and IL-8 revealed that these chemokines also significantly suppressed HIV-1 replication in infected macrophages. RANTES is able to induce desensitization and internalization of CCR5, the chemokine coreceptor of R5 variants. In addition, IL-8 receptor activation results in cross-desensitization and cross-internalization of CCR5. We found that CCR5 sensitivity and expression level is diminished in HHV-6A-infected macrophage cultures compared with uninfected cells. Taken together, our results indicate that HHV-6A infection decreases the susceptibility of macrophages to R5 variants of HIV-1 in which the HHV-6A induced RANTES and IL-8 may have importance.
- Published
- 2006
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16. Significant decrease of the enhancement/neutralization index in HIV patients during highly active antiretroviral therapy (HAART).
- Author
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Bánhegyi D, Bácsi A, Tóth FD, Prohászka Z, Horváth A, Beck Z, Kónya J, and Füst G
- Subjects
- Antibodies, Blocking blood, Biomarkers blood, CD4 Lymphocyte Count, CD8-Positive T-Lymphocytes immunology, Complement System Proteins immunology, Drug Therapy, Combination, Follow-Up Studies, HIV Antibodies blood, HIV Infections immunology, HIV Infections virology, Humans, Lymphocyte Count, Male, Middle Aged, Neutralization Tests, Antibodies, Blocking immunology, Antiretroviral Therapy, Highly Active, HIV Antibodies immunology, HIV Infections drug therapy, HIV-1 growth & development, HIV-1 immunology
- Abstract
Authors studied the effect of highly active antiretroviral therapy (HAART) on balance of the antibodies that enhance or neutralize growth of HIV-1(IIIB) strain in MT-4 cells in the presence or absence of human complement. Sequential serum samples were collected from 28 patients in advanced stage of HIV disease before and during HAART. The balance of the enhancing and neutralizing antibodies was expressed by an index value (E/N I). Samples with an E/N I of <0.5 (twofold decrease in virus production) were considered as neutralizing, whereas samples with an E/N I>2.0 (twofold increase in virus production) were considered as enhancing. At the beginning of HAART serum samples from eight patients enhanced, and samples from only two patients neutralized the virus in the presence of complement, median (25th-75th percentile) value of E/N I was 1.32 (0.79-2.29). E/N I significantly (P<0.0001) dropped to 0.37 (0.19-0.57) during the follow-up period of 18.5 (10.5-23.5) months under HAART. Similar changes were detected when serum samples were tested with no complement added. The E/N I values were also markedly decreased when cultures inoculated with mixtures of HIV and purified IgG prepared from serum pools taken before and during HAART, respectively, were compared. In the last samples of 20/28 patients, neutralization was measured even in the presence of complement while enhancement was found with none of these samples. These findings suggest that HAART results in disappearance of enhancing antibodies and switches the E/N I toward neutralization.
- Published
- 2003
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17. Structural basis for distinctions between substrate and inhibitor specificities for feline immunodeficiency virus and human immunodeficiency virus proteases.
- Author
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Lin YC, Beck Z, Morris GM, Olson AJ, and Elder JH
- Subjects
- Amino Acid Sequence, Amino Acid Substitution, Animals, Aspartic Acid Endopeptidases genetics, Binding Sites, Cats, HIV Protease genetics, HIV Protease Inhibitors pharmacology, HIV-1 genetics, Humans, Immunodeficiency Virus, Feline genetics, Molecular Sequence Data, Structure-Activity Relationship, Substrate Specificity, Aspartic Acid Endopeptidases chemistry, Aspartic Acid Endopeptidases metabolism, HIV Protease chemistry, HIV Protease metabolism, HIV-1 enzymology, Immunodeficiency Virus, Feline enzymology
- Abstract
We used feline immunodeficiency virus (FIV) protease (PR) as a mutational framework to define determinants for the observed substrate and inhibitor specificity distinctions between FIV and human immunodeficiency virus (HIV) PRs. Multiple-substitution mutants were constructed by replacing the residues in and around the active site of FIV PR with the structurally equivalent residues of HIV-1 PR. Mutants included combinations of three critical regions (FIV numbering, with equivalent HIV numbering in superscript): I37(32)V in the active core region; N55(46)M, M56(47)I, and V59(50)I in the flap region; and L97(80)T, I98(81)P, Q99(82)V, P100(83)N, and L101(84)I in the 90s loop region. Significant alterations in specificity were observed, consistent with the involvement of these residues in determining the substrate-inhibitor specificity distinctions between FIV and HIV PRs. Two previously identified residues, I35 and I57 of FIV PR, were intolerant to substitution and yielded inactive PRs. Therefore, we attempted to recover the activity by introducing secondary mutations. The addition of G62(53)F and K63(54)I, located at the top of the flap and outside the active site, compensated for the activity lost in the I57(48)G substitution mutants. An additional two substitutions, D105(88)N and N88(74)T, facilitated recovery of activity in mutants that included the I35(30)D substitution. Determination of K(i) values of potent HIV-1 PR inhibitors against these mutants showed that inhibitor specificity paralleled that of HIV-1 PR. The findings indicate that maintenance of both substrate and inhibitor specificity is a function of interactions between residues both inside and outside the active site. Thus, mutations apparently peripheral to the active site can have a dramatic influence on inhibitor efficacy.
- Published
- 2003
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18. Human herpesvirus 6 variant a infects human term syncytiotrophoblasts in vitro and induces replication of human immunodeficiency virus type 1 in dually infected cells.
- Author
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Csoma E, Bácsi A, Liu X, Szabó J, Ebbesen P, Beck Z, Kónya J, Andirkó I, Nagy E, and Tóth FD
- Subjects
- Cells, Cultured, Female, Genetic Variation, HIV-1 genetics, Herpesvirus 6, Human isolation & purification, Humans, Immunophenotyping, Placenta cytology, Pregnancy, Receptors, CCR3, Receptors, CXCR4 metabolism, Receptors, Chemokine metabolism, Transfection, Trophoblasts cytology, Trophoblasts virology, HIV-1 physiology, Herpesvirus 6, Human physiology, Placenta virology, Virus Replication
- Abstract
Human herpesvirus 6 (HHV-6) and human immunodeficiency virus type 1 (HIV-1) may interact during transplacental transmission of HIV-1. The placental syncytiotrophoblast layer serves as the first line of defense of the fetus against viruses. Patterns of replication of HHV-6 variant A (HHV-6A) and HIV-1 were analyzed in singly and dually infected human term syncytiotrophoblast cells cultured in vitro. For this purpose, the GS strain of HHV-6A and the Ba-L and IIIB strains of HIV-1 were used. HHV-6A replication was restricted at the level of early gene products in singly infected syncytiotrophoblasts, whereas no viral protein expression was found in cells infected with HIV-1 alone. Coinfection of syncytiotrophoblast cells with HHV-6A and HIV-1 resulted in production of infectious HIV-1. In contrast, no enhancement of HHV-6A expression was observed in cell cultures infected with both viruses. Uninfected syncytiotrophoblast cells were found to express CXCR4 and CCR3 but not CD4 or CCR5 receptors. Infection of syncytiotrophoblasts with HHV-6A did not induce CD4 expression and had no influence on chemokine receptor expression. Activation of HIV-1 from latency in coinfected cells was mediated by the immediate-early (IE)-A and IE-B gene products of HHV-6A. Open reading frames U86 and U89 of the IE-A region were able to activate HIV-1 replication in a synergistic manner. The data suggest that in vivo double infection of syncytiotrophoblast cells with HHV-6A and HIV-1 could contribute to the transplacental transmission of HIV-1 but not HHV-6A., (Copyright 2002 Wiley-Liss, Inc.)
- Published
- 2002
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19. Induction of HIV-1 replication in latently infected syncytiotrophoblast cells by contact with placental macrophages: role of interleukin-6 and tumor necrosis factor-alpha.
- Author
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Bácsi A, Csoma E, Beck Z, Andirkó I, Kónya J, Gergely L, and Tóth FD
- Subjects
- Antibodies pharmacology, Cells, Cultured, Coculture Techniques, Gene Products, tat metabolism, HIV Core Protein p24 metabolism, HIV-1 metabolism, Humans, Interleukin-6 antagonists & inhibitors, Interleukin-6 immunology, Interleukin-6 physiology, Kinetics, Microscopy, Fluorescence, Tumor Necrosis Factor-alpha antagonists & inhibitors, Tumor Necrosis Factor-alpha immunology, Tumor Necrosis Factor-alpha physiology, Virus Replication, tat Gene Products, Human Immunodeficiency Virus, Cytokines physiology, HIV-1 growth & development, Macrophages immunology, Placenta immunology, Trophoblasts virology
- Abstract
The syncytiotrophoblast (ST) layer of the human placenta has an important role in limiting transplacental viral spread from mother to fetus. Although certain strains of human immunodeficiency virus type 1 (HIV-1) may enter ST cells, the trophoblast does not exhibit permissiveness for HIV-1. The present study tested the possibility that placental macrophages might induce replication of HIV-1 carried in ST cells and, further, that infected ST cells would be capable of transmitting virus into neighboring macrophages. For this purpose, we investigated HIV-1 replication in ST cells grown alone or cocultured with uninfected placental macrophages. The macrophage-tropic Ba-L strain of HIV-1, capable of entering ST cells, was used throughout our studies. We demonstrated that interactions between ST cells and macrophages activated HIV-1 from latency and induced its replication in ST cells. After having become permissive for viral replication, ST cells delivered HIV-1 to the cocultured macrophages, as evidenced by detection of virus-specific antigens in these cells. The stimulatory effect of coculture on HIV-1 gene expression in ST cells was mediated by marked tumor necrosis factor-alpha (TNF-alpha) and interleukin-6 (IL-6) release from macrophages, an effect caused by contact between the different placental cells. Results of this study suggest an interactive role for the ST layer and placental macrophages in the dissemination of HIV-1 among placental tissue. Data reported here may also explain why macrophage-tropic HIV-1 strains are transmitted preferentially during pregnancy.
- Published
- 2001
- Full Text
- View/download PDF
20. Molecular basis for the relative substrate specificity of human immunodeficiency virus type 1 and feline immunodeficiency virus proteases.
- Author
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Beck ZQ, Lin YC, and Elder JH
- Subjects
- Amino Acid Substitution, Animals, Cats, Endopeptidases genetics, HIV-1 genetics, Humans, Immunodeficiency Virus, Feline genetics, Protein Conformation, Substrate Specificity, Viral Proteins genetics, Viral Proteins metabolism, Endopeptidases metabolism, HIV-1 enzymology, Immunodeficiency Virus, Feline enzymology
- Abstract
We have used a random hexamer phage library to delineate similarities and differences between the substrate specificities of human immunodeficiency virus type 1 (HIV-1) and feline immunodeficiency virus (FIV) proteases (PRs). Peptide sequences were identified that were specifically cleaved by each protease, as well as sequences cleaved equally well by both enzymes. Based on amino acid distinctions within the P3-P3' region of substrates that appeared to correlate with these cleavage specificities, we prepared a series of synthetic peptides within the framework of a peptide sequence cleaved with essentially the same efficiency by both HIV-1 and FIV PRs, Ac-KSGVF/VVNGLVK-NH(2) (arrow denotes cleavage site). We used the resultant peptide set to assess the influence of specific amino acid substitutions on the cleavage characteristics of the two proteases. The findings show that when Asn is substituted for Val at the P2 position, HIV-1 PR cleaves the substrate at a much greater rate than does FIV PR. Likewise, Glu or Gln substituted for Val at the P2' position also yields peptides specifically susceptible to HIV-1 PR. In contrast, when Ser is substituted for Val at P1', FIV PR cleaves the substrate at a much higher rate than does HIV-1 PR. In addition, Asn or Gln at the P1 position, in combination with an appropriate P3 amino acid, Arg, also strongly favors cleavage by FIV PR over HIV PR. Structural analysis identified several protease residues likely to dictate the observed specificity differences. Interestingly, HIV PR Asp30 (Ile-35 in FIV PR), which influences specificity at the S2 and S2' subsites, and HIV-1 PR Pro-81 and Val-82 (Ile-98 and Gln-99 in FIV PR), which influence specificity at the S1 and S1' subsites, are residues which are often involved in development of drug resistance in HIV-1 protease. The peptide substrate KSGVF/VVNGK, cleaved by both PRs, was used as a template for the design of a reduced amide inhibitor, Ac-GSGVF Psi(CH(2)NH)VVNGL-NH(2.) This compound inhibited both FIV and HIV-1 PRs with approximately equal efficiency. These findings establish a molecular basis for distinctions in substrate specificity between human and feline lentivirus PRs and offer a framework for development of efficient broad-based inhibitors.
- Published
- 2001
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21. Pseudotypes of vesicular stomatitis virus-bearing envelope antigens of certain HIV-1 strains permissively infect human syncytiotrophoblasts cultured in vitro: implications for in vivo infection of syncytiotrophoblasts by cell-free HIV-1.
- Author
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Bácsi A, Ebbesen P, Szabó J, Beck Z, Andirkó I, Csoma E, and Tóth FD
- Subjects
- Antibodies, Monoclonal pharmacology, CD4 Antigens immunology, Cell Line, Cytopathogenic Effect, Viral, HIV Antigens immunology, HIV Infections transmission, HIV-1 genetics, Humans, Immune Sera pharmacology, Infectious Disease Transmission, Vertical, Macrophages virology, Receptors, CCR3, Receptors, CCR5 immunology, Receptors, CXCR4 immunology, Receptors, Chemokine immunology, T-Lymphocytes virology, Time Factors, Trophoblasts drug effects, U937 Cells, Vesicular stomatitis Indiana virus genetics, Vesicular stomatitis Indiana virus immunology, Virus Replication, HIV-1 immunology, Trophoblasts virology, Vesicular stomatitis Indiana virus physiology
- Abstract
Intrauterine infection of the fetus is clearly an important mode of vertical transmission of human immunodeficiency virus type 1 (HIV-1). The syncytiotrophoblast layer of the human placenta must be traversed by HIV-1 in order to reach underlying cells and fetal capillaries. Although HIV-1 has been detected in the syncytiotrophoblast layer in situ, there is conflicting evidence regarding infection of syncytiotrophoblast cells with cell-free virus. The phenotypic mixing between HIV-1 and vesicular stomatitis virus (VSV) has been exploited to assay the susceptibility of human term syncytiotrophoblast cells to penetration by various strains of HIV-1. VSV(HIV-1(IIIB)) and VSV(HIV-1(Ba-L)) pseudotypes were found to enter syncytiotrophoblast cells. In contrast, VSV pseudotyped with envelope glycoproteins of RF, MN, or Ada-M strains of HIV-1 did not infect syncytiotrophoblasts. Plating efficiency of VSV(HIV-1(IIIB)) and VSV(HIV-1(Ba-L)) was 10-fold lower on syncytiotrophoblasts than on T-cells and macrophages, respectively. Incubation of VSV(HIV-1(IIIB)) and VSV(HIV-1(Ba-L)) viruses with appropriate HIV-1 neutralizing sera before infection strongly inhibited entry of pseudotyped VSV into syncytiotrophoblast cells. These findings demonstrated that infection of syncytiotrophoblasts with VSV(HIV-1) pseudotypes was mediated by Env from IIIB and Ba-L strains of HIV-1. Monoclonal antibodies (MAb) to CD4, CXCR4, CCR5, and CCR3 were tested for their ability to block VSV(HIV-1) infection of syncytiotrophoblast cells. Neither the anti-CD4 nor the anti-CXCR4, anti-CCR5, and anti-CCR3 MAb had any inhibitory effect on infection of syncytiotrophoblast cells with VSV(HIV-1) pseudotypes. Results from this study suggest that cell-free HIV-1 can enter syncytiotrophoblasts and the susceptibility of these cells to penetration by the virus is strain dependent. Pseudotype infection merely demonstrates that the first steps in HIV-1 replication are possible in syncytiotrophoblast cells., (Copyright 2001 Wiley-Liss, Inc.)
- Published
- 2001
- Full Text
- View/download PDF
22. Soluble gC1q-R/p33, a cell protein that binds to the globular "heads" of C1q, effectively inhibits the growth of HIV-1 strains in cell cultures.
- Author
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Szabó J, Cervenák L, Tóth FD, Prohászka Z, Horváth L, Kerekes K, Beck Z, Bácsi A, Erdei A, Peerschke EI, Füst G, and Ghebrehiwet B
- Subjects
- Anti-HIV Agents chemistry, Anti-HIV Agents pharmacology, Binding Sites, CD4 Antigens metabolism, Carrier Proteins, Cell Line, Complement C1q chemistry, HIV Envelope Protein gp120 metabolism, HIV Infections drug therapy, HIV Infections immunology, HIV Infections virology, HIV-1 pathogenicity, Humans, Macrophages drug effects, Macrophages immunology, Macrophages virology, Mitochondrial Proteins, Recombinant Proteins metabolism, Recombinant Proteins pharmacology, Solubility, T-Lymphocytes drug effects, T-Lymphocytes immunology, T-Lymphocytes virology, Virus Replication drug effects, Complement C1q metabolism, HIV-1 drug effects, HIV-1 physiology, Hyaluronan Receptors, Membrane Glycoproteins, Receptors, Complement administration & dosage, Receptors, Complement metabolism
- Abstract
C1q and the outer envelope protein of HIV, gp120, have several structural and functional similarities. Therefore, it is plausible to assume that proteins that are able to interact with C1q may also interact with isolated gp120 as well as the whole HIV-1 virus. Based on this hypothesis, we studied the potential ability of the recombinant form of the 33-kDa protein, which binds to the globular "heads" of C1q (gC1q-R/p33), to inhibit the growth of different HIV-1 strains in cell cultures. gC1q-R/p33 was found to effectively and dose-dependently inhibit the production of one T-lymphotropic (X4) and one macrophage-tropic (R5) strain in human T cell lines (MT-4 and H9) and human monocyte-derived macrophage cultures, respectively. At a concentration range of 5-25 microg/ml, gC1q-R caused a marked and prolonged suppression of virus production. The extent of inhibition was enhanced when gC1q-R was first incubated with and then removed from the target cell cultures before virus infection, compared to that when the cells were infected with gC1q-R-HIV mixtures. The extent of inhibition was comparable to that of the Leu3a anti-CD4 antibody. Addition of gC1q-R to the cell cultures on day 1 or 2 after infection induced markedly less inhibition of HIV-1 growth than pretreatment of the cells just before or together with the infective HIV strains. In ELISA experiments, gC1q-R did not bind to a solid-phase recombinant gp120 while strong and dose-dependent binding of gC1q-R to solid-phase CD4 was observed. Our present findings indicate that gC1q-R is an effective inhibitor of HIV-1 infection, which prevents viral entry by blocking the interaction between CD4 and gp120. Since gC1q-R is a human protein, it is most probably not antigenic in humans. It would seem logical, therefore, to consider gC1q-R or its fragments involved in the CD4 binding as potential therapeutic agents., (Copyright 2001 Academic Press.)
- Published
- 2001
- Full Text
- View/download PDF
23. Identification of efficiently cleaved substrates for HIV-1 protease using a phage display library and use in inhibitor development.
- Author
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Beck ZQ, Hervio L, Dawson PE, Elder JH, and Madison EL
- Subjects
- Amides chemistry, Amides metabolism, Amides pharmacology, Amino Acid Sequence, Drug Resistance, Microbial, HIV Integrase chemistry, HIV Integrase metabolism, HIV Protease Inhibitors chemistry, HIV Protease Inhibitors pharmacology, HIV Reverse Transcriptase chemistry, HIV Reverse Transcriptase metabolism, Hydrogen-Ion Concentration, Kinetics, Peptide Fragments genetics, Protein Binding, Reducing Agents metabolism, Substrate Specificity, Thermodynamics, HIV Protease metabolism, HIV Protease Inhibitors metabolism, HIV-1 enzymology, Peptide Fragments chemistry, Peptide Fragments metabolism, Peptide Library
- Abstract
The recognition sequences for substrate cleavage by aspartic protease of HIV-1 are diverse and cleavage specificities are controlled by complex interactions between at least six amino acids around the cleavage site. We have identified 45 efficiently cleaved peptide substrates of HIV-1 protease (PR) using substrate phage display, an approach that can elucidate both context-dependent and context-independent preferences at individual subsites of a protease substrate. Many of the selected peptides were cleaved more efficiently and had lower K(m) values than physiologically relevant substrates of HIV-1 PR. Therefore, mutations occurring in the cleavage sites of the Gag and Gag-pol polyproteins of HIV-1 could significantly lower the K(m) values to better compete against drugs for protease binding while maintaining cleavage rates necessary for viral replication. The most efficiently cleaved peptide substrate derived from these phage, Ac-GSGIF*LETSL-NH(2), was cleaved 60 times more efficiently and had a K(m) approximately 260 times lower than a nine-amino-acid peptide based on the natural reverse transcriptase/integrase cleavage site when assayed at pH 5.6, 0.2 M NaCl. The peptide substrates selected served as frameworks for synthesis of tight binding reduced amide inhibitors of HIV-1 PR. The results show that the most efficiently cleaved substrates serve as the best templates for synthesis of the tightest binding inhibitors. Thus, defining changes in substrate preferences for drug-resistant proteases may aid in the development of more efficacious inhibitors., (Copyright 2000 Academic Press.)
- Published
- 2000
- Full Text
- View/download PDF
24. Differential patterns of interaction between HIV type 1 and HTLV type I in monocyte-derived macrophages cultured in vitro: implications for in vivo coinfection with HIV type 1 and HTLV type I.
- Author
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Szabó J, Beck Z, Csomán E, Liu X, Andrikó I, Kiss J, Bácsi A, Ebbesen P, and Tóth FD
- Subjects
- Cell Nucleus metabolism, Cells, Cultured, DNA, Viral metabolism, Gene Products, tat physiology, Gene Products, tax physiology, Genes, pX, Genes, tat, HIV Infections complications, HIV-1 genetics, HTLV-I Infections complications, Human T-lymphotropic virus 1 genetics, Humans, Polymerase Chain Reaction, Proviruses genetics, Transfection, Tumor Cells, Cultured, Virus Replication, tat Gene Products, Human Immunodeficiency Virus, HIV-1 physiology, Human T-lymphotropic virus 1 physiology, Macrophages virology, Viral Interference
- Abstract
The interaction between human immunodeficiency virus type 1 (HIV-1) and human T cell leukemia-lymphoma virus type I (HTLV-I) has generated substantial interest. However, there is disagreement on the in vivo consequences of the double infection. We investigated the interactions between HIV-1 and HTLV-I in monocyte-derived macrophages cultured in vitro. For study, the T cell-tropic strain IIIB and the macrophagetropic strain Ada-M of HIV-1 were used. The HTLV-I was prepared from the supernatants of the virus-producing MT-2 cell line. We found that coinfection of macrophages with T cell-tropic HIV-1 and HTLV-I significantly enhanced HIV-1 replication, whereas double infection of the cells with macrophage-tropic HIV-1 and HTLV-I resulted in marked upregulation of HTLV-I production. Stimulatory interactions between HIV-1 and HTLV-I were mediated by their trans-acting proteins. Results of study on nuclear translocation of proviral DNA showed that the tax gene product of HTLV-I was able to facilitate the nuclear import of the reverse-transcribed HIV-1(IIIB) DNA. In contrast, the HIV-1 Tat protein did not increase the intranuclear trafficking of HTLV-I DNA, which suggests another mechanism for HTLV-I enhancement by the tat gene product. In conclusion, this study provides possible mechanisms whereby coinfection of an individual with HIV-1 and HTLV-I may influence the clinical outcome of double infection.
- Published
- 1999
- Full Text
- View/download PDF
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