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Infection of human peripheral blood mononuclear cells by erythrocyte-bound HIV-1: effects of antibodies and complement.
- Source :
-
Virology [Virology] 2011 Apr 10; Vol. 412 (2), pp. 441-7. Date of Electronic Publication: 2011 Feb 21. - Publication Year :
- 2011
-
Abstract
- Two human monoclonal antibodies, 4E10 and b12, were examined for antibody-dependent neutralization, or antibody-dependent complement (C)-mediated neutralization, of infection of peripheral blood mononuclear cells (PBMC) by either free HIV-1 or trans infection by HIV bound to erythrocytes. Neutralization of free HIV-1 by b12 was stronger than by 4E10, but b12 neutralized erythrocyte-bound HIV-1 less efficiently than cell-free virus. 4E10 did not neutralize erythrocyte-bound HIV-1 and at a low concentration it caused enhancement of infection. Antibody (4E10)-dependent C activation inhibited trans infection by erythrocyte-bound HIV-1, but caused enhanced infection with cell-free HIV-1 in the presence of erythrocytes. No effects of C were observed with b12. C-dependent enhancement in the presence of erythrocytes is proposed as due to binding of C3b-4E10-cell-free-HIV or C3d-4E10-cell-free-HIV to C receptor type 1 (CR1) on erythrocytes, or C receptor type 2 (CR2) on B cells in the PBMC, followed by trans infection of susceptible cells.<br /> (Published by Elsevier Inc.)
- Subjects :
- Antibodies, Neutralizing immunology
Antibody-Dependent Enhancement
HIV Infections immunology
HIV-1 immunology
Humans
Neutralization Tests
Virus Attachment
Complement System Proteins immunology
Erythrocytes virology
HIV Antibodies immunology
HIV Infections virology
HIV-1 pathogenicity
Leukocytes, Mononuclear virology
Subjects
Details
- Language :
- English
- ISSN :
- 1096-0341
- Volume :
- 412
- Issue :
- 2
- Database :
- MEDLINE
- Journal :
- Virology
- Publication Type :
- Academic Journal
- Accession number :
- 21334707
- Full Text :
- https://doi.org/10.1016/j.virol.2011.01.037