1. Expression and function of murine receptor tyrosine kinases, TIE and TEK, in hematopoietic stem cells.
- Author
-
Yano M, Iwama A, Nishio H, Suda J, Takada G, and Suda T
- Subjects
- Animals, Antibodies, Monoclonal immunology, Bone Marrow, COS Cells, Cell Differentiation, Cell Line, Colony-Forming Units Assay, Enzyme Induction, Mice, Mice, Inbred C57BL, Peptide Fragments genetics, Peptide Fragments immunology, Phosphorylation, Protein Processing, Post-Translational, Rats, Rats, Wistar, Receptor Protein-Tyrosine Kinases genetics, Receptor Protein-Tyrosine Kinases immunology, Receptor, TIE-2, Receptors, TIE, Recombinant Fusion Proteins immunology, Signal Transduction, Hematopoietic Stem Cells enzymology, Receptor Protein-Tyrosine Kinases physiology
- Abstract
Two highly related receptor tyrosine kinases, TIE and TEK, comprise a family of endothelial cell-specific kinase. We established monoclonal antibodies against them and performed detailed analyses on their expression and function in murine hematopoietic stem cells (HSCs). TIE and TEK were expressed on 23.7% and 33.3% of lineage marker-negative, c-Kit+ and Sca-1+ (Lin- c-Kit+ Sca-1+) HSCs that contain the majority of day-12 colony-forming units-spleen (CFU-S) and long-term reconstituting cells, but not committed progenitor cells. Lin- c-Kit+ Sca-1+ cells were further divided by the expression of TIE and TEK. TIE+ and TEK+ HSCs as well as each negative counterpart contained high proliferative potential colony-forming cells and differentiated into lymphoid and myeloid progenies both in vitro and in vivo. However, day-12 CFU-S were enriched in TIE+ and TEK+ HSCs. Our findings define TIE and TEK as novel stem cell marker antigens that segregate day-12 CFU-S, and provide evidence of novel signaling pathways that are involved in the functional regulation of HSCs at a specific stage of differentiation, particularly of day-12 CFU-S.
- Published
- 1997