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Effects of recombinant murine granulocyte colony-stimulating factor on granulocyte-macrophage and blast colony formation.
- Source :
-
Experimental hematology [Exp Hematol] 1987 Oct; Vol. 15 (9), pp. 958-65. - Publication Year :
- 1987
-
Abstract
- We performed the present study to define the in vitro hemopoietic activity of murine recombinant (r) granulocyte colony-stimulating factor (G-CSF) using murine hemopoietic culture systems of normal bone marrow cells, fetal liver cells, and spleen cells of 5-fluorouracil (FU)-treated mice. Recombinant G-CSF supported only neutrophil and/or macrophage colony formation by normal bone marrow cells. It did not enhance the formation of erythroid bursts in the fetal liver cell assay, but interleukin-3 (IL-3) did. Paradoxically, rG-CSF could support the colony formation of multilineage colonies as well as blast colonies from the spleen cells of 5-FU-treated mice, while r-granulocyte-macrophage colony-stimulating factor (GM-CSF) and r-erythropoietin (Ep) did not. When blast colonies, formed in the presence of G-CSF, were replated to dishes containing IL-3, they were able to differentiate along multilineage pathways. However, when they were replated to dishes containing rG-CSF, they could differentiate only into neutrophils and macrophages. Single cells transferred from blast colonies formed only neutrophil-macrophage colonies. These data indicate that rG-CSF had a direct effect on the growth and development of GM progenitors at a late stage and a significant effect on multipotential hemopoietic precursors. Although it remains to be clarified how G-CSF acts on multipotential stem cells, this unique effect is important in the understanding of its pluripotent hemopoietic activity in vivo.
- Subjects :
- Animals
Cell Differentiation drug effects
Colony-Forming Units Assay
Erythropoietin pharmacology
Female
Granulocytes
Hematopoietic Stem Cells cytology
Interleukin-3 pharmacology
Macrophages
Mice
Mice, Inbred ICR
Pregnancy
Recombinant Fusion Proteins pharmacology
Colony-Stimulating Factors pharmacology
Hematopoietic Stem Cells drug effects
Subjects
Details
- Language :
- English
- ISSN :
- 0301-472X
- Volume :
- 15
- Issue :
- 9
- Database :
- MEDLINE
- Journal :
- Experimental hematology
- Publication Type :
- Academic Journal
- Accession number :
- 3498640