1. Innovative Preparation of Biopharmaceuticals Using Transglycosylation Activity of Microbial Endoglycosidases
- Author
-
Kenji Yamamoto and Toshihiko Katoh
- Subjects
chemistry.chemical_classification ,Glycan ,biology ,Chemistry ,endo-β-N-acetylglucosaminidase ,glycoengineering ,carbohydrates (lipids) ,immunoglobulin G ,Biopharmaceutical ,Biochemistry ,Homogeneous ,Regular Paper ,biology.protein ,Functional studies ,Glycoprotein ,transglycosylation ,remodeling - Abstract
Most functional biopharmaceuticals such as antibodies are glycoproteins carrying N-linked oligosaccharides (N-glycans). In animal cells, these glycans are generally expressed as heterogeneous glycoforms that are difficult to separate into a pure form. The structure of these glycans directly affects several biological aspects of the glycoproteins, especially binding affinity. Therefore, the preparation of glycoproteins with well-defined and homogeneous glycoforms is necessary for functional studies and improved efficacy, particularly for biopharmaceuticals. This review describes the recent remarkable progress in the development and production of biopharmaceutical glycan-modified antibodies, through the use of glycan remodeling using microbial endoglycosidases and sophisticated glycoengineering techniques utilizing microbial enzymatic reaction mechanisms.
- Published
- 2021