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Chemo-enzymatic synthesis of a glycosylated peptide containing a complex N-glycan based on unprotected oligosaccharides by using DMT-MM and Endo-M
- Source :
- Glycoconjugate journal. 34(4)
- Publication Year :
- 2017
-
Abstract
- For chemo-enzymatic synthesis of a glycosylated peptide, 4-(4,6-dimethoxy-1,3,5-triazin-2-yl)-4-methylmorpholinium chloride (DMT-MM) was used for the synthesis of a N-acetylglucosaminyl peptide and a pseudoglycopeptide by solid-phase peptide synthesis without the requirement of protecting groups on the carbohydrate. We also performed transglycosylation of an N-glycan to the N-acetylglucosaminyl peptide using endo-β-N-acetylglucosaminidase from Mucor hiemalis (Endo-M) to synthesize a glycopeptide containing a complex N-glycan.
- Subjects :
- 0301 basic medicine
Glycan
Glycosylation
Time Factors
Stereochemistry
Morpholines
Oligosaccharides
Peptide
Chemo enzymatic
01 natural sciences
Biochemistry
Chloride
03 medical and health sciences
chemistry.chemical_compound
Acetylglucosaminidase
medicine
Peptide synthesis
Molecular Biology
chemistry.chemical_classification
biology
010405 organic chemistry
Chemistry
Cell Biology
Carbohydrate
biology.organism_classification
Glycopeptide
0104 chemical sciences
030104 developmental biology
Mucor hiemalis
Mucor
biology.protein
Biocatalysis
Peptides
medicine.drug
Subjects
Details
- ISSN :
- 15734986
- Volume :
- 34
- Issue :
- 4
- Database :
- OpenAIRE
- Journal :
- Glycoconjugate journal
- Accession number :
- edsair.doi.dedup.....17812020ab13710574a7a167b52fc495