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For simultaneous measurement of glucose, lactate, glutamine, and glutamate a biosensor array is implemented in a micro flow-system thus giving a microsystem. The microsystem consists of a glass chip with the integrated biosensor array and a bottom part, which comprises a gold counter electrode, a 300 microm thick seal, and electrical interconnection lines. The flow device has a total internal volume of 2.1 or 6 microl when integrated with a mixer on chip. The biosensors with no crosstalking and high long term stability were produced by modifying the electrochemical transducers and utilizing photopatternable enzyme membranes. The use of appropriate miniaturization technology leads to mass producable devices for in vivo and ex vivo applications in whole blood and fermentation broth. Due to a novel glutaminase with an activity optimum in the neutral pH range direct and simultaneous monitoring of glutamine together with glucose, lactate, and glutamate could be performed.

Bioprocess development and fermentation control would benefit from a timely knowledge of metabolic parameters, e.g., glucose, glutamine, and lactate concentration etc., enabling adequate intervention, maximizing product yield, and providing process analytical tools for reliable and traceable bioprocessing in the context of process analytical technology. A polymer laminate technology based microsytem comprising integrated pre-calibrated electrochemical biosensors for the simultaneous monitoring of lactate, glucose, glutamine, and glutamate in a 0.15-μL flow-through cell for process monitoring and control is presented. Flexible manufacturing technologies used for these devices allow a cost effective customization of them. [ABSTRACT FROM AUTHOR]

For simultaneous measurement of glucose, lactate, glutamine, and glutamate a biosensor array is implemented in a micro flow-system thus giving a microsystem. The microsystem consists of a glass chip with the integrated biosensor array and a bottom part, which comprises a gold counter electrode, a 300 μm thick seal, and electrical interconnection lines. The flow device has a total internal volume of 2.1 or 6 μl when integrated with a mixer on chip. The biosensors with no crosstalking and high long term stability were produced by modifying the electrochemical transducers and utilizing photopatternable enzyme membranes. The use of appropriate miniaturization technology leads to mass producable devices for in vivo and ex vivo applications in whole blood and fermentation broth. Due to a novel glutaminase with an activity optimum in the neutral pH range direct and simultaneous monitoring of glutamine together with glucose, lactate, and glutamate could be performed. [Copyright &y& Elsevier]