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Biosensor arrays for simultaneous measurement of glucose, lactate, glutamate, and glutamine.
- Source :
-
Biosensors & bioelectronics [Biosens Bioelectron] 2002 Apr; Vol. 17 (4), pp. 297-302. - Publication Year :
- 2002
-
Abstract
- For simultaneous measurement of glucose, lactate, glutamine, and glutamate a biosensor array is implemented in a micro flow-system thus giving a microsystem. The microsystem consists of a glass chip with the integrated biosensor array and a bottom part, which comprises a gold counter electrode, a 300 microm thick seal, and electrical interconnection lines. The flow device has a total internal volume of 2.1 or 6 microl when integrated with a mixer on chip. The biosensors with no crosstalking and high long term stability were produced by modifying the electrochemical transducers and utilizing photopatternable enzyme membranes. The use of appropriate miniaturization technology leads to mass producable devices for in vivo and ex vivo applications in whole blood and fermentation broth. Due to a novel glutaminase with an activity optimum in the neutral pH range direct and simultaneous monitoring of glutamine together with glucose, lactate, and glutamate could be performed.
- Subjects :
- Biosensing Techniques methods
Enzymes, Immobilized metabolism
Equipment Design
Glucose metabolism
Glutamic Acid metabolism
Glutamine metabolism
Lactic Acid metabolism
Membranes, Artificial
Microelectrodes
Polyhydroxyethyl Methacrylate chemistry
Biosensing Techniques instrumentation
Glucose analysis
Glutamic Acid analysis
Glutamine analysis
Lactic Acid analysis
Subjects
Details
- Language :
- English
- ISSN :
- 0956-5663
- Volume :
- 17
- Issue :
- 4
- Database :
- MEDLINE
- Journal :
- Biosensors & bioelectronics
- Publication Type :
- Academic Journal
- Accession number :
- 11849926
- Full Text :
- https://doi.org/10.1016/s0956-5663(01)00298-6