Search

The Gram-strain-negative, rod-shaped, facultatively anaerobic, non-motile bacterial strain, designated S1-10 T , was isolated from marine sediment. Strain S1-10 T grew at 4-42 °C (optimally at 30-35 °C), at pH 7.0-10 (optimally at pH 9) and in the presence of 0.5-8 % (w/v) NaCl. Phylogenetic analysis based on 16S rRNA gene sequences showed that strain S1-10 T was related to the genus Aequorivita and had highest 16S rRNA gene sequence similarity to Aequorivita viscosa 8-1b T (97.7%). The predominant cellular fatty acids were iso-C 15 : 0 and anteiso-C 15 : 0 . The main respiratory quinone was menaquinone 6 (MK-6). The genomic DNA G+C content of strain S1-10 T was 34.6 mol%. The polar lipid profile of strain S1-10 T contained phosphatidylethanolamine, two aminolipids, two glycolipids, one phosphoglycolipid and three unidentified polar lipids. In addition, the maximum values of in silico DNA-DNA hybridization ( is DDH) and average nucleotide identity (ANI) between strain S1-10 T and A. viscosa CGMCC 1.11023 T were 15.4 and 75.7 %, respectively. Combined data from phenotypic, phylogenetic, is DDH and ANI analyses demonstrated that strain S1-10 T is the representative of a novel species of the genus Aequorivita , for which we propose the name Aequorivita sinensis sp. nov. (type strain S1-10 T =CGMCC 1.12579 T =JCM 19789 T ). We also propose that Vitellibacter todarodis and Vitellibacter aquimaris should be transferred into genus Aequorivita and be named Aequorivita todarodis comb. nov. and Aequorivita aquimaris comb. nov., respectively. The type strain of Aequorivita todarodis comb. nov. is MYP2-2 T (= KCTC 62141 T = NBRC 113025 T ) and the type strain of Aequorivita aquimaris comb. nov. is D-24 T (=KCTC 42708 T =DSM 101732 T ).

A Gram-stain negative, rod-shaped, facultative anaerobic, motile bacterial strain, designated TP187 T , was isolated from a seamount near the Yap Trench in the tropical western Pacific. Phylogenetic analysis based on the 16S rRNA gene sequence showed that strain TP187 T is related to members of the genus Vibrio and has high 16S rRNA gene sequence similarity with the type strains of Vibrio chagasii (97.3%) and Vibrio gallaecicus (97.1%). Sequence similarities to all other type strains of current species of the genus Vibrio were below 97%. The polar lipids profile was found to contain diphosphatidylglycerol, phosphatidylglycerol, an aminophospholipid, two aminolipids, four phospholipids and eleven unidentified polar lipids. Ubiquinone Q-8 was detected as the predominant quinone. The genomic DNA G + C content of strain TP187 T was determined to be 43.7 mol%. In addition, the maximum values of in silico DNA-DNA hybridization (isDDH) and average nucleotide identity (ANI) between strain TP187 T with V. chagasii LMG 21353 T were 22.40 and 77.50% respectively. Both values are below the proposed cutoff levels for species delineation, i.e. 70 and 95%, respectively. Combined data from phenotypic, phylogenetic, isDDH and ANI data demonstrated that the strain TP187 T is representative of a novel species of the genus Vibrio, for which we propose the name Vibrio profundi sp. nov. (type strain TP187 T  = KACC 18555 T  = CGMCC 1.15395 T ).

A Gram-stain negative, rod-shaped, strictly aerobic, motile bacterial strain, designated YC239 T , was isolated from a seamount near the Yap Trench in the tropical western Pacific. Phylogenetic analysis based on 16S rRNA gene sequence showed that strain YC239 T is related to the genus Ponticaulis and has high 16S rRNA gene sequence similarity with the type strain of Ponticaulis koreensis GSW-23 T (97.9%). The predominant cellular fatty acids were C 18:1 ω7c, C 16:0 , C 17:0 and C 17:1 ω6c. Strain YC239 T had Q-10 as the predominant ubiquinone. The polar lipid profile contained phosphatidylglycerol, two unidentified aminolipids and six unidentified polar lipids. The genomic DNA G+C content of strain YC239 T was 52.8 mol%. Strain YC239 T shared DNA relatedness of 38% with Ponticaulis koreensis KCTC 22146 T . On the basis of the evidence presented in this study, strain YC239 T represents a novel species of the genus Ponticaulis, for which we propose the name Ponticaulis profundi sp. nov. (type strain YC239 T  = KACC 19027 T  = CGMCC 1.15741 T ).

A Gram-stain-negative, rod-shaped, yellow-pigmented, gliding bacterial strain, designated S5-23-3T, was isolated from a sediment sample of the Yellow Sea in China. Phylogenetic analysis based on 16S rRNA gene sequences showed that strain S5-23-3T was related to the genus Winogradskyella and had highest 16S rRNA gene sequence similarities with Winogradskyella arenosi JCM 15527T (97.6 %), Winogradskyella rapida CECT 7392T (97.4 %) and Winogradskyella undariae KCTC 32261T (97.2 %). The predominant cellular fatty acids were iso-C15 : 0, iso-C15 : 1 G, iso-C15 : 0 3-OH and iso-C17 : 0 3-OH. Strain S5-23-3T contained MK-6 as the predominant menaquinone. The polar lipid profile contained phosphatidylethanolamine, two aminolipids, one aminoglycolipid, one aminophospholipid, one unidentified phospholipid and seven unidentified polar lipids. The genomic DNA G+C content of strain S5-23-3T was 36.1 mol%. Combined data from phenotypic, chemotaxonomic, phylogenetic and DNA-DNA relatedness studies demonstrated that strain S5-23-3T is a representative of a novel species of the genus Winogradskyella, for which the name Winogradskyellasediminis sp. nov. (type strain S5-23-3T=LMG 28075T=DSM 28134T) is proposed.

The Gram-stain-negative, yellow-pigmented, rod-shaped bacterial strain, designated S11-41(T), was isolated from a sediment sample of the Yellow Sea in China. The strain was able to grow well over a wide temperature range (1-35 °C). Phylogenetic analysis based on 16S rRNA gene sequences showed that strain S11-41(T) was related to the members of the genus Gelidibacter, sharing highest sequence similarities with the type strains of Geldibacter salicanalis (98.2%), Geldibacter algens (97.4%) and Geldibacter mesophilus (96.9%). The genomic DNA G+C content of strain S11-41(T) was 39.2 mol%. The predominant cellular fatty acids were iso-C15 : 0 (13.2%), iso-C17 : 0 3-OH (12.8%) and iso-C15 : 1 G (12.2%). Phosphatidylethanolamine was identified as the major polar lipid. Combined data from phenotypic, phylogenetic and DNA-DNA relatedness studies demonstrated that strain S11-41(T) is a representative of a novel species of the genus Gelidibacter, for which we propose the name Gelidibacter sediminis sp. nov. (type strain S11-41(T)= DSM 28135(T) = LMG 28076(T)).

A Gram-reaction-negative, rod-shaped, yellow-pigmented, motile by gliding bacterial strain, designated RU-4-M-4(T), was isolated from intertidal sediment of Sakhalin Island in Russia. Phylogenetic analysis based on 16S rRNA gene sequences showed that strain RU-4-M-4(T) was related to the genus Algibacter and had highest 16S rRNA gene sequence similarity with Algibacter pectinivorans KACC 14153(T) (97.2%). The major cellular fatty acids were iso-C15 : 0 3-OH, C15: 0 and iso-C15 : 1 G. The predominant menaquinone was MK-6. The polar lipid profile contained phosphatidylethanolamine, three unidentified aminolipids and two unidentified lipids. The genomic DNA G+C content of strain RU-4-M-4(T) was 36.4 mol%. Combined data from phenotypic, phylogenetic and DNA-DNA relatedness studies demonstrated that strain RU-4-M-4(T) is a representative of a novel species of the genus Algibacter , for which we propose the name Algibacter amylolyticus sp. nov. (type strain RU-4-M-4(T) =LMG 28383(T) =DSM 29199(T))., (© 2015 IUMS.)

A Gram-stain-negative, Na(+)-requiring bacterial strain, designated B20-1(T), was isolated from soil of the root system of mangrove forest. Cells were curved rods and motile by means of a polar flagellum. Phylogenetic analysis based on 16S rRNA gene sequences showed that strain B20-1(T) belonged to the genus Marinomonas , sharing highest sequence similarities with Marinomonas rhizomae IVIA-Po-145(T) (97.6%), Marinomonas dokdonensis DSW10-10(T) (97.0%) and Marinomonas foliarum IVIA-Po-155(T) (96.9%). The predominant cellular fatty acids of strain B20-1(T) were C10 : 0 3-OH, C18 : 1ω7c, summed feature 3 (C16 : 1ω7c and/or iso-C15 : 0 2-OH) and C16 : 0. Phosphatidylethanolamine and phosphatidylglycerol were identified as the predominant phospholipids. The predominant ubiquinone was Q-8. The genomic DNA G+C content of strain B20-1(T) was 46.6 mol%. On the basis of phenotypic characteristics, phylogenetic analysis and DNA-DNA relatedness, a novel species, Marinomonas mangrovi sp. nov., is proposed with B20-1(T) ( =DSM 28136(T) =LMG 28077(T)) as the type strain., (© 2015 IUMS.)

The Gram-stain-negative, rod-shaped bacterial strain designated RU-1-R-18(T) was isolated from intertidal sediment on Sakhalin Island in Russia. Phylogenetic analysis based on 16S rRNA gene sequences showed that strain RU-1-R-18(T) was related to the genus Puniceibacterium and shared highest sequence similarities with the type strain Puniceibacterium antarcticum KACC 16875(T) (97.9%). The predominant cellular fatty acid was C(18:1)ω7c. The polar lipid profile contained diphosphatidylglycerol, phosphatidylglycerol, phosphatidylethanolamine, phosphatidylcholine, one unidentified aminophospholipid and seven unidentified polar lipids. The genomic DNA G+C content of strain RU-1-R-18(T) was 59.1 mol%. Combined data from phenotypic, phylogenetic and DNA-DNA relatedness studies demonstrated that strain RU-1-R-18(T) represents a novel species of the genus Puniceibacterium , for which the name Puniceibacterium sediminis sp. nov. is proposed (type strain RU-1-R-18(T) =LMG 28384(T) =DSM 29052(T))., (© 2015 IUMS.)

Two Gram-stain-negative, non-motile, rod-shaped bacterial strains, designated BM5-7(T) and BM9-1(T) were isolated from soil of the root system of a mangrove forest. Phylogenetic analysis based on 16S rRNA gene sequences showed that the two isolates belong to the genus Martelella. The chemotaxonomic characteristics of these isolates included the presence of C19 : 0 cyclo ω8c and C18 : 1ω7c as the major cellular fatty acids and Q-10 as the dominant ubiquinone. The genomic DNA G+C contents of strains BM5-7(T) and BM9-1(T) were 61.0 and 59.7 mol% (HPLC method), respectively. The 16S rRNA gene sequence similarity between the two strains was 98.1 %, but DNA-DNA hybridization indicated 44 % relatedness. Strains BM5-7(T) and BM9-1(T) exhibited 16S rRNA gene sequence similarities of 98.0-99.2 % and 97.7-98.1 %, respectively, with type strains of Martelella endophytica and Martelella mediterranea. Combined data from phenotypic, phylogenetic and DNA-DNA relatedness studies demonstrated that strains BM5-7(T) and BM9-1(T) are representatives of two novel species of the genus Martelella, for which the names Martelella radicis sp. nov. (type strain BM5-7(T) = DSM 28101(T) = LMG 27958(T)) and Martelella mangrovi sp. nov. (type strain BM9-1(T) = DSM 28102(T) = LMG 27959(T)) are proposed., (© 2014 IUMS.)

A Gram-negative, psychrophilic, motile, rod-shaped bacterium, designated strain 20188(T), was isolated from marine samples collected from the Arctic (7 degrees 00' 24'' N 16 degrees 59' 37'' W), and was identified taxonomically by means of a polyphasic study. On the basis of 16S rRNA gene sequence similarity, strain 20188(T) was closely related to members of the genera Phaeobacter. 16S rRNA gene sequence similarities between strain 20188(T) and the type strains of Phaeobacter inhibens, Phaeobacter gallaeciensis and Phaeobacter daeponensis were 97.0, 96.8 and 96.2 %, respectively. The temperature range for growth was 0-25 degrees C, with optimum growth occurring at 19-20 degrees C and at approximately pH 6.0-9.0. Strain 20188(T) had ubiquinone-10 as the major respiratory quinone and C(18 : 1)omega7c and 11-methyl C(18 : 1)omega7c as major fatty acids. The genomic DNA G+C content was 59.6 mol%. On the basis of phenotypic characteristics, phylogenetic analysis and DNA-DNA relatedness data, strain 20188(T) is considered to represent a novel species of the genus Phaeobacter, for which the name Phaeobacter arcticus sp. nov. is proposed. The type strain is 20188(T) (=CGMCC 1.6500(T)=JCM 14644(T)).