1. Moving forward one step back at a time: reversibility during homologous recombination
- Author
-
Piazza, Aurèle and Heyer, Wolf-Dietrich
- Subjects
Biochemistry and Cell Biology ,Biological Sciences ,Genetics ,Underpinning research ,1.1 Normal biological development and functioning ,Generic health relevance ,DNA Breaks ,Double-Stranded ,DNA Helicases ,DNA-Binding Proteins ,DNA-Directed DNA Polymerase ,Endodeoxyribonucleases ,Recombinational DNA Repair ,Saccharomyces cerevisiae ,D-loop ,Homologous recombination ,Helicase ,Homology search ,Genomic stability ,Crossover ,Microbiology - Abstract
DNA double-strand breaks are genotoxic lesions whose repair can be templated off an intact DNA duplex through the conserved homologous recombination (HR) pathway. Because it mainly consists of a succession of non-covalent associations of molecules, HR is intrinsically reversible. Reversibility serves as an integral property of HR, exploited and tuned at various stages throughout the pathway with anti- and pro-recombinogenic consequences. Here, we focus on the reversibility of displacement loops (D-loops), a central DNA joint molecule intermediate whose dynamics and regulation have recently been physically probed in somatic S. cerevisiae cells. From homology search to repair completion, we discuss putative roles of D-loop reversibility in repair fidelity and outcome.
- Published
- 2019