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Your search keyword '"Daddiego, Loretta"' showing total 5 results
Start Over Author "Daddiego, Loretta" Topic genetics
5 results on '"Daddiego, Loretta"'

1. The transcriptional response to the olive fruit fly (Bactrocera oleae) reveals extended differences between tolerant and susceptible olive (Olea europaea L.) varieties.

Author

Grasso, Filomena, Coppola, Mariangela, Carbone, Fabrizio, Baldoni, Luciana, Alagna, Fiammetta, Perrotta, Gaetano, Pérez-Pulido, Antonio J., Garonna, Antonio, Facella, Paolo, Daddiego, Loretta, Lopez, Loredana, Vitiello, Alessia, Rao, Rosa, and Corrado, Giandomenico

Subjects
OLIVE fly, OLIVE diseases & pests, OLIVE varieties, GENE expression in plants, PYROSEQUENCING
Abstract

The olive fruit fly Bactrocera oleae (Diptera: Tephritidae) is the most devastating pest of cultivated olive (Olea europaea L.). Intraspecific variation in plant resistance to B. oleae has been described only at phenotypic level. In this work, we used a transcriptomic approach to study the molecular response to the olive fruit fly in two olive cultivars with contrasting level of susceptibility. Using next-generation pyrosequencing, we first generated a catalogue of more than 80,000 sequences expressed in drupes from approximately 700k reads. The assembled sequences were used to develop a microarray layout with over 60,000 olive-specific probes. The differential gene expression analysis between infested (i.e. with II or III instar larvae) and control drupes indicated a significant intraspecific variation between the more tolerant and susceptible cultivar. Around 2500 genes were differentially regulated in infested drupes of the tolerant variety. The GO annotation of the differentially expressed genes implies that the inducible resistance to the olive fruit fly involves a number of biological functions, cellular processes and metabolic pathways, including those with a known role in defence, oxidative stress responses, cellular structure, hormone signalling, and primary and secondary metabolism. The difference in the induced transcriptional changes between the cultivars suggests a strong genetic role in the olive inducible defence, which can ultimately lead to the discovery of factors associated with a higher level of tolerance to B. oleae. [ABSTRACT FROM AUTHOR]

Published
2017
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2. Pyrosequencing Unveils Cystic Fibrosis Lung Microbiome Differences Associated with a Severe Lung Function Decline.

Author

Bacci, Giovanni, Paganin, Patrizia, Lopez, Loredana, Vanni, Chiara, Dalmastri, Claudia, Cantale, Cristina, Daddiego, Loretta, Perrotta, Gaetano, Dolce, Daniela, Morelli, Patrizia, Tuccio, Vanessa, De Alessandri, Alessandra, Fiscarelli, Ersilia Vita, Taccetti, Giovanni, Lucidi, Vincenzina, Bevivino, Annamaria, and Mengoni, Alessio

Subjects
CYSTIC fibrosis, LUNG microbiology, PYROSEQUENCING, PSEUDOMONAS, BACTERIAL communities, STATISTICAL correlation
Abstract

Chronic airway infection is a hallmark feature of cystic fibrosis (CF) disease. In the present study, sputum samples from CF patients were collected and characterized by 16S rRNA gene-targeted approach, to assess how lung microbiota composition changes following a severe decline in lung function. In particular, we compared the airway microbiota of two groups of patients with CF, i.e. patients with a substantial decline in their lung function (SD) and patients with a stable lung function (S). The two groups showed a different bacterial composition, with SD patients reporting a more heterogeneous community than the S ones. Pseudomonas was the dominant genus in both S and SD patients followed by Staphylococcus and Prevotella. Other than the classical CF pathogens and the most commonly identified non-classical genera in CF, we found the presence of the unusual anaerobic genus Sneathia. Moreover, the oligotyping analysis revealed the presence of other minor genera described in CF, highlighting the polymicrobial nature of CF infection. Finally, the analysis of correlation and anti-correlation networks showed the presence of antagonism and ecological independence between members of Pseudomonas genus and the rest of CF airways microbiota, with S patients showing a more interconnected community in S patients than in SD ones. This population structure suggests a higher resilience of S microbiota with respect to SD, which in turn may hinder the potential adverse impact of aggressive pathogens (e.g. Pseudomonas). In conclusion, our findings shed a new light on CF airway microbiota ecology, improving current knowledge about its composition and polymicrobial interactions in patients with CF. [ABSTRACT FROM AUTHOR]

Published
2016
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3. Transcript Analysis and Regulative Events during Flower Development in Olive (Olea europaea L.).

Author

Alagna, Fiammetta, Cirilli, Marco, Galla, Giulio, Carbone, Fabrizio, Daddiego, Loretta, Facella, Paolo, Lopez, Loredana, Colao, Chiara, Mariotti, Roberto, Cultrera, Nicolò, Rossi, Martina, Barcaccia, Gianni, Baldoni, Luciana, Muleo, Rosario, and Perrotta, Gaetano

Subjects
OLIVE, FLOWER development, PLANT reproduction, PLANT metabolism, PHENOTYPES
Abstract

The identification and characterization of transcripts involved in flower organ development, plant reproduction and metabolism represent key steps in plant phenotypic and physiological pathways, and may generate high-quality transcript variants useful for the development of functional markers. This study was aimed at obtaining an extensive characterization of the olive flower transcripts, by providing sound information on the candidate MADS-box genes related to the ABC model of flower development and on the putative genetic and molecular determinants of ovary abortion and pollen-pistil interaction. The overall sequence data, obtained by pyrosequencing of four cDNA libraries from flowers at different developmental stages of three olive varieties with distinct reproductive features (Leccino, Frantoio and Dolce Agogia), included approximately 465,000 ESTs, which gave rise to more than 14,600 contigs and approximately 92,000 singletons. As many as 56,700 unigenes were successfully annotated and provided gene ontology insights into the structural organization and putative molecular function of sequenced transcripts and deduced proteins in the context of their corresponding biological processes. Differentially expressed genes with potential regulatory roles in biosynthetic pathways and metabolic networks during flower development were identified. The gene expression studies allowed us to select the candidate genes that play well-known molecular functions in a number of biosynthetic pathways and specific biological processes that affect olive reproduction. A sound understanding of gene functions and regulatory networks that characterize the olive flower is provided. [ABSTRACT FROM AUTHOR]

Published
2016
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4. The coffee genome provides insight into the convergent evolution of caffeine biosynthesis.

Author

Denoeud, France, Carretero-Paulet, Lorenzo, Dereeper, Alexis, Droc, Gaëtan, Guyot, Romain, Pietrella, Marco, Zheng, Chunfang, Alberti, Adriana, Anthony, François, Aprea, Giuseppe, Aury, Jean-Marc, Bento, Pascal, Bernard, Maria, Bocs, Stéphanie, Campa, Claudine, Cenci, Alberto, Combes, Marie-Christine, Crouzillat, Dominique, Da Silva, Corinne, and Daddiego, Loretta

Subjects
*PLANT genomes, *COFFEE, *METHYLTRANSFERASE genetics, *CAFFEINE, *COMPARATIVE genomics, *BIOLOGICAL evolution, *GENETICS
Abstract

The article discusses the authors' generation of a high-quality draft genome of a coffee species that displays a conserved chromosomal gene order among asterid angiosperms, called Coffea canephora. The authors identified several species-specific gene family expansions, such as N-methyltransferases (NMTs), which is involved in caffeine production, and performed a comparative analysis of caffeine NMTs that suggests the genes arose independently of genes from cacao and tea.

Published
2014
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5. The coffee genome provides insight into the convergent evolution of caffeine biosynthesis

Author

Giorgio Graziosi, Maria Bernard, Michel Rigoreau, Adriana Muñoz, Olivier Garsmeur, Alan Carvalho Andrade, Qiong Zhang, Priyono, Xavier Argout, Lorenzo Carretero-Paulet, Thomas Gayraud, Giovanni Giuliano, Alexandre de Kochko, Steve Rounsley, Alberto Pallavicini, Benoît Bertrand, David Sankoff, Claudine Campa, Dominique Crouzillat, Patrick Wincker, Loretta Daddiego, François Anthony, Marie Christine Combes, Romain Guyot, Valentin Guignon, Katharina Jahn, Loredana Lopez, Pascal Bento, Stéphane Dussert, Christine Tranchant-Dubreuil, Maud Lepelley, Tianying Lan, Ray Ming, Thierry Joët, Gaëtan Droc, G. Aprea, Corinne Da Silva, Julio Rozas, David Pot, Véronique Jamilloux, Thierry Leroy, Benjamin Noel, Valérie Poncet, Pablo Librado, Adriana Alberti, Victor A. Albert, Jean-Marc Aury, Gaetano Perrotta, Philippe Lashermes, Robert VanBuren, Chunfang Zheng, Robert J Henry, Leiting Li, Julie Leclercq, Chifumi Nagai, Marco Pietrella, Karine Labadie, Jayarama, Stéphanie Bocs, Alberto Cenci, Fabien De Bellis, Mathieu Rouard, Alexis Dereeper, Génomique métabolique (UMR 8030), Genoscope - Centre national de séquençage [Evry] (GENOSCOPE), Université Paris-Saclay-Direction de Recherche Fondamentale (CEA) (DRF (CEA)), Commissariat à l'énergie atomique et aux énergies alternatives (CEA)-Commissariat à l'énergie atomique et aux énergies alternatives (CEA)-Université Paris-Saclay-Direction de Recherche Fondamentale (CEA) (DRF (CEA)), Commissariat à l'énergie atomique et aux énergies alternatives (CEA)-Commissariat à l'énergie atomique et aux énergies alternatives (CEA)-Université d'Évry-Val-d'Essonne (UEVE)-Centre National de la Recherche Scientifique (CNRS), Université d'Évry-Val-d'Essonne (UEVE), Institut de Génomique d'Evry (IG), Université Paris-Saclay-Institut de Biologie François JACOB (JACOB), Direction de Recherche Fondamentale (CEA) (DRF (CEA)), Commissariat à l'énergie atomique et aux énergies alternatives (CEA)-Commissariat à l'énergie atomique et aux énergies alternatives (CEA)-Direction de Recherche Fondamentale (CEA) (DRF (CEA)), Commissariat à l'énergie atomique et aux énergies alternatives (CEA)-Commissariat à l'énergie atomique et aux énergies alternatives (CEA), University at Buffalo [SUNY] (SUNY Buffalo), State University of New York (SUNY), Institut de Recherche pour le Développement (IRD), Résistance des plantes aux bio-agresseurs (UMR RPB), Centre de Coopération Internationale en Recherche Agronomique pour le Développement (Cirad)-Université Montpellier 2 - Sciences et Techniques (UM2), Amélioration génétique et adaptation des plantes méditerranéennes et tropicales (UMR AGAP), Centre de Coopération Internationale en Recherche Agronomique pour le Développement (Cirad)-Institut National de la Recherche Agronomique (INRA)-Centre international d'études supérieures en sciences agronomiques (Montpellier SupAgro)-Institut national d’études supérieures agronomiques de Montpellier (Montpellier SupAgro), Diversité, adaptation, développement des plantes (UMR DIADE), Centre de Coopération Internationale en Recherche Agronomique pour le Développement (Cirad)-Université de Montpellier (UM)-Centre National de la Recherche Scientifique (CNRS)-Institut de Recherche pour le Développement (IRD [France-Sud]), Agenzia Nazionale per le nuove Tecnologie, l’energia e lo sviluppo economico sostenibile = Italian National Agency for New Technologies, Energy and Sustainable Economic Development (ENEA), Department of Mathematics and Statistics [Ottawa], University of Ottawa [Ottawa], Commissariat à l'énergie atomique et aux énergies alternatives (CEA), Bioversity, Parc Scientifique Agropolis II, Nestlé France, Center for Biotechnology (CeBiTec), Universität Bielefeld = Bielefeld University, Unité de Recherche Génomique Info (URGI), Institut National de la Recherche Agronomique (INRA), Chongqing University of Science & Technology, University of Illinois at Urbana-Champaign [Urbana], University of Illinois System, Institut de Recerca de la Biodiversitat - Biodiversity Research Institute [Barcelona, Spain] (IRBio UB), Universitat de Barcelona (UB), Department of Mathematics [College Park], University of Maryland [College Park], University of Maryland System-University of Maryland System, Università degli studi di Trieste = University of Trieste, Indonesian Coffee and Cocoa Research Institute, Partenaires INRAE, Embrapa Recursos Genéticos e Biotecnologia [Brasília], Queensland Alliance for Agriculture and Food Innovation (QAAFI), University of Queensland [Brisbane], Hawaii Agriculture Research Center, University of Arizona, Australian Research Council, Natural Sciences and Engineering Research Council of Canada, CNR-ENEA Agrifood Project A2 C44 L191, FINEP-Qualicafe, NSF 0922742 0922545, College of Arts and Sciences, University at Buffalo, SouthGreen Bioinformatics platform (UMR AGAP), CIRAD, INCT-CAFE, ANR-08-GENM-022-001, ANR-09-GENM-014-002, ANR-08-GENM-0022,GénomeCafé,Analyses de séquences d'extrémités de clones BAC comme étape préliminaire au séquençage du génome des caféiers(2008), ANR-09-GENM-0014,COFFEASEQ,Séquençage du génome du caféier (Coffea canephora)(2009), Institut de Biologie François JACOB (JACOB), Commissariat à l'énergie atomique et aux énergies alternatives (CEA)-Commissariat à l'énergie atomique et aux énergies alternatives (CEA)-Université Paris-Saclay, Université Montpellier 2 - Sciences et Techniques (UM2)-Centre de Coopération Internationale en Recherche Agronomique pour le Développement (Cirad), Institut national d'enseignement supérieur pour l'agriculture, l'alimentation et l'environnement (Institut Agro)-Institut national d'enseignement supérieur pour l'agriculture, l'alimentation et l'environnement (Institut Agro), Institut de Recherche pour le Développement (IRD [France-Sud])-Centre National de la Recherche Scientifique (CNRS)-Université de Montpellier (UM)-Centre de Coopération Internationale en Recherche Agronomique pour le Développement (Cirad), Italian National Agency for New Technologies, Energy and Sustainable Economic Development (ENEA), Department of Mathematics, University of Maryland, University of Trieste, Giuliano, G., Perrotta, G., Lopez, Daddiego, L., Aprea, G., Pietrella, M., Denoeud, France, Carretero Paulet, Lorenzo, Dereeper, Alexi, Droc, Gaëtan, Guyot, Romain, Pietrella, Marco, Zheng, Chunfang, Alberti, Adriana, Anthony, Françoi, Aprea, Giuseppe, Aury, Jean Marc, Bento, Pascal, Bernard, Maria, Bocs, Stéphanie, Campa, Claudine, Cenci, Alberto, Combes, Marie Christine, Crouzillat, Dominique, Da Silva, Corinne, Daddiego, Loretta, De Bellis, Fabien, Dussert, Stéphane, Garsmeur, Olivier, Gayraud, Thoma, Guignon, Valentin, Jahn, Katharina, Jamilloux, Véronique, Joët, Thierry, Labadie, Karine, Lan, Tianying, Leclercq, Julie, Lepelley, Maud, Leroy, Thierry, Li, Lei Ting, Librado, Pablo, Lopez, Loredana, Muñoz, Adriana, Noel, Benjamin, Pallavicini, Alberto, Perrotta, Gaetano, Poncet, Valérie, Pot, David, Priyono, P., Rigoreau, Michel, Rouard, Mathieu, Rozas, Julio, Tranchant Dubreuil, Christine, Vanburen, Robert, Zhang, Qiong, Andrade, Alan C., Argout, Xavier, Bertrand, Benoît, De Kochko, Alexandre, Graziosi, Giorgio, Henry, Robert J., Jayarama, J., Ming, Ray, Nagai, Chifumi, Rounsley, Steve, Sankoff, David, Giuliano, Giovanni, Albert, Victor A., Wincker, Patrick, and Lashermes, Philippe

Subjects
Phylogénie, [SDV]Life Sciences [q-bio], Coffea, Évolution, biosynthèse, Genome, Camellia sinensis, F30 - Génétique et amélioration des plantes, chemistry.chemical_compound, Solanum lycopersicum, genetics, Methyltransferase, Phylogeny, Plant Proteins, caffeine, 2. Zero hunger, Genetics, Multidisciplinary, Medicine (all), Plant Protein, food and beverages, F70 - Taxonomie végétale et phytogéographie, Caféine, Caffeine, Genome, Plant, coffea, Canephora, F60 - Physiologie et biochimie végétale, Biology, Transférase, Coffea canephora, Evolution, Molecular, Phylogenetics, evolution, Methyltransferases, Gene family, [SDV.BV]Life Sciences [q-bio]/Vegetal Biology, Theobroma cacao, Gene, genome, Génome, Molecular, Plant, biology.organism_classification, chemistry, Angiosperme
Abstract

Coffee, tea, and chocolate converge Caffeine has evolved multiple times among plant species, but no one knows whether these events involved similar genes. Denoeud et al. sequenced the Coffea canephora (coffee) genome and identified a conserved gene order (see the Perspective by Zamir). Although this species underwent fewer genome duplications than related species, the relevant caffeine genes experienced tandem duplications that expanded their numbers within this species. Scientists have seen similar but independent expansions in distantly related species of tea and cacao, suggesting that caffeine might have played an adaptive role in coffee evolution. Science , this issue p. 1181 ; see also p. 1124

Published
2014
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