1. Viral Engineering of Chimeric Antigen Receptor Expression on Murine and Human T Lymphocytes.
- Author
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Hammill JA, Afsahi A, Bramson JL, and Helsen CW
- Subjects
- Animals, Cell Culture Techniques, Gene Transfer Techniques, Humans, Immunotherapy, Adoptive, Lentivirus genetics, Mice, Neoplasms immunology, Neoplasms therapy, Receptors, Antigen, T-Cell metabolism, Recombinant Fusion Proteins metabolism, Retroviridae genetics, Transduction, Genetic, Genetic Engineering, Genetic Vectors genetics, Receptors, Antigen, T-Cell genetics, Recombinant Fusion Proteins genetics, T-Lymphocytes immunology, T-Lymphocytes metabolism
- Abstract
The adoptive transfer of a bolus of tumor-specific T lymphocytes into cancer patients is a promising therapeutic strategy. In one approach, tumor specificity is conferred upon T cells via engineering expression of exogenous receptors, such as chimeric antigen receptors (CARs). Here, we describe the generation and production of both murine and human CAR-engineered T lymphocytes using retroviruses.
- Published
- 2016
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