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The CD8+ T cell population elicited by recombinant adenovirus displays a novel partially exhausted phenotype associated with prolonged antigen presentation that nonetheless provides long-term immunity.
- Source :
-
Journal of immunology (Baltimore, Md. : 1950) [J Immunol] 2006 Jan 01; Vol. 176 (1), pp. 200-10. - Publication Year :
- 2006
-
Abstract
- We have previously reported that the CD8+ T cell response elicited by recombinant adenovirus vaccination displayed a delayed contraction in the spleen. In our current study, we demonstrate that this unusual kinetic is a general phenomenon observed in multiple tissues. Phenotypic analysis of transgene-specific CD8+ T cells present 30 days postimmunization with recombinant adenovirus revealed a population with evidence of partial exhaustion, suggesting that the cells had been chronically exposed to Ag. Although Ag expression could no longer be detected 3 wk after immunization, examination of Ag presentation within the draining lymph nodes demonstrated that APCs were loaded with Ag peptide for at least 40 days postimmunization, suggesting that Ag remains available to the system for a prolonged period, although the exact source of this Ag remains to be determined. At 60 days postimmunization, the CD8+ T cell population continued to exhibit a phenotype consistent with partially exhausted effector memory cells. Nonetheless, these CD8+ T cells conferred sterilizing immunity against virus challenge 7-12 wk postimmunization, suggesting that robust protective immunity can be provided by CD8+ T cells with an exhausted phenotype. These data demonstrate that prolonged exposure to Ag may not necessarily impair protective immunity and prompt a re-evaluation of the impact of persistent exposure to Ag on T cell function.
- Subjects :
- Adenoviridae genetics
Adoptive Transfer
Animals
Antigens, Viral immunology
Egg Proteins immunology
Female
Flow Cytometry
Genetic Vectors administration & dosage
Immunophenotyping
Lymph Nodes immunology
Mice
Ovalbumin immunology
Peptide Fragments
Time Factors
Transgenes
Adenoviridae immunology
Antigen Presentation immunology
CD8-Positive T-Lymphocytes immunology
Genetic Vectors immunology
Subjects
Details
- Language :
- English
- ISSN :
- 0022-1767
- Volume :
- 176
- Issue :
- 1
- Database :
- MEDLINE
- Journal :
- Journal of immunology (Baltimore, Md. : 1950)
- Publication Type :
- Academic Journal
- Accession number :
- 16365411
- Full Text :
- https://doi.org/10.4049/jimmunol.176.1.200