7 results on '"Chushu Zhang"'
Search Results
2. Pseudomonas laoshanensis sp. nov., isolated from peanut field soil
- Author
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Song Yu, Lina Yu, Chushu Zhang, Xiao-Yuan Chi, Yang Weiqiang, Wang Mingqing, Zhang Jiancheng, Jiao Kun, Gong Kuijie, Qingli Yang, Jie Sun, Jie Bi, and Gui-Wen Yang
- Subjects
0303 health sciences ,biology ,Phylogenetic tree ,030306 microbiology ,Pseudomonas ,General Medicine ,biology.organism_classification ,16S ribosomal RNA ,rpoB ,Biochemistry ,Microbiology ,Pseudomonas pertucinogena ,03 medical and health sciences ,genomic DNA ,Genetics ,Molecular Biology ,Gene ,Bacteria ,030304 developmental biology - Abstract
A novel Gram-stain-negative, aerobic strain, designated Y22T, was isolated from peanut field soil in Laoshan Mountain in China. Cells of strain Y22T were rod-shaped and motile by a single flagellum. The strain was found to be oxidase- and catalase-positive. 16S rRNA gene sequence based on phylogenetic analysis indicated that strain Y22T belonged to the genus Pseudomonas, and showed the highest 16S rRNA gene sequence similarity of 99.0% to Pseudomonas pelagia JCM 15562T, followed by Pseudomonas salina JCM 19469T (98.4%), Pseudomonas sabulinigri JCM 14963T (97.9%), Pseudomonas bauzanensis CGMCC 1.9095T (97.6%) and Pseudomonas litoralis KCTC23093T (97.5%). The phylogenetic analysis based on multilocus sequence analyses with concatenated 16S rRNA, gyrB, rpoD and rpoB genes indicated that strain Y22T belonged to Pseudomonas pertucinogena lineage. The average nucleotide identity scores between strain Y22T and closely related species were 74.6–82.8%, and the Genome-to-Genome Distance Calculator scores were 16.4–44.9%. The predominant cellular fatty acids of strain Y22T were C18:1ω7c (29.6%), C17:0 cyclo (17.5%) and summed feature 3 (C16:1ω7c and/or C16:1ω6c) (17.4%). The genomic DNA G+C content was 57.9 mol%. On the basis of phenotypic characteristics, phylogenetic analyses and in silico DNA–DNA relatedness, a novel species, Pseudomonas laoshanensis sp. nov. is proposed. The type strain is Y22T (= JCM 32580T = KCTC 62385T = CGMCC 1.16552T).
- Published
- 2020
3. Pseudomonas qingdaonensis sp. nov., an aflatoxin-degrading bacterium, isolated from peanut rhizospheric soil
- Author
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Zhen Wang, Jie Sun, Lina Yu, Chushu Zhang, Wang Mingqing, and Jie Bi
- Subjects
DNA, Bacterial ,Aflatoxin ,China ,Aflatoxin B1 ,Arachis ,Sequence analysis ,Biochemistry ,Microbiology ,03 medical and health sciences ,Soil ,Pseudomonas ,RNA, Ribosomal, 16S ,Genetics ,Molecular Biology ,Phospholipids ,Phylogeny ,Soil Microbiology ,030304 developmental biology ,0303 health sciences ,Base Composition ,Phylogenetic tree ,biology ,Strain (chemistry) ,030306 microbiology ,Fatty Acids ,Nucleic Acid Hybridization ,General Medicine ,Sequence Analysis, DNA ,biology.organism_classification ,16S ribosomal RNA ,Catalase ,Pseudomonas putida ,Bacterial Typing Techniques ,Genes, Bacterial ,Oxidoreductases ,Bacteria ,Multilocus Sequence Typing - Abstract
A Gram-stain-negative, aerobic, mobile, and rod-shaped bacterium, designated JJ3T, was isolated from peanut rhizospheric soil in Qingdao, Shandong Province, China, and was characterized using a polyphasic approach. Strain JJ3T grew at 4–40 °C, at pH 5.0–9.0 and 0–4% NaCl. The strain was positive for both catalase and oxidase tests, and was able to degrade aflatoxin B1. According to the 16S rRNA gene sequence comparisons, the strain JJ3T was identified as a member of the genus Pseudomonas and was most closely related to Pseudomonas japonica JCM 21532T and Pseudomonas alkylphenolica JCM 16553T with sequence similarity of 99.0% and 98.9%, respectively. A multilocus sequence analysis (MLSA) of concatenating 16S rRNA, gyrB and rpoD gene sequences showed that strain JJ3T belonged to the Pseudomonas putida subcluster. Genomic comparison of strain JJ3T with its closest phylogenetic type strain using average nucleotide index (ANI) and digital DNA–DNA relatedness revealed 76.7–82.9% and 20.2–37.1%, respectively. All values were distinctly lower than the thresholds established for species differentiation. The predominant cellular fatty acids of strain JJ3T were C17:0 cyclo (24.0%), C16:0 (21.4%), summed features 3 (C16:1ω7c and/or C16:1ω6c) (11.5%) and summed features 8 (C18:1ω7c and/or C18:1ω6c) (10.5%). The major polar lipids of strain JJ3T were phosphatidylethanolamine, phosphatidylglycerol and diphosphatidylglycerol. The physiological, biochemical, and genetic characteristics support the assignment of JJ3T to the genus Pseudomonas, but are different to those of phylogenetically neighboring species to represent a novel species. The name Pseudomonas qingdaonensis sp. nov. is proposed, with JJ3T (= JCM 32579T = KCTC 62384T = CGMCC 1.16493T) as the type strain.
- Published
- 2018
4. Nutritional composition and protein quality of the edible beetle Holotrichia parallela
- Author
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Qingli Yang, Lina Yu, Jie Sun, Yang Zhen, Jie Bi, Shaofang Liu, and Chushu Zhang
- Subjects
protein quality ,China ,nutritional composition ,Linoleic acid ,Chitin ,Scarabaeoidea ,Biology ,Holotrichia parallela ,chemistry.chemical_compound ,Botany ,Animals ,Food science ,Threonine ,Amino Acids ,amino acid composition ,chemistry.chemical_classification ,Research ,beetle ,Fatty Acids ,General Medicine ,biology.organism_classification ,Amino acid ,Trace Elements ,Coleoptera ,Oleic acid ,chemistry ,Insect Science ,Insect Proteins ,Dietary Proteins ,Protein quality ,Nutritive Value - Abstract
The adult edible beetle Holotrichia parallela Motschulsky (Coleoptera: Scarabaeoidea) represents a traditional food source in China. Based on nutritional analyses, adult H. parallela is high in protein (70%) and minerals and low in fat. H. parallela contained approximately 10% chitin; the corrected protein content was 66%. Oleic acid and linoleic acid were the most abundant fatty acids. Of the total amino acids in H. parallela , 47.4% were essential amino acids. The amino acid scores were 87 and 100, based on the corrected crude and net protein contents, respectively; threonine was the limiting amino acid. In vitro protein digestibility was 78%, and the protein digestibility-corrected amino acid score was 89 based on the net protein content. Adult H. parallela may be a potential source of proteins and minerals for humans and animals.
- Published
- 2014
5. Preparation, characterisation and physicochemical properties of the phosphate modified peanut protein obtained from Arachin Conarachin L
- Author
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Chushu Zhang, Jie Bi, Yang Weiqiang, Jie Sun, Lina Yu, and Qingli Yang
- Subjects
Chromatography ,Arachis ,Chemical Phenomena ,Sodium ,Microwave power ,Protein isolate ,Temperature ,chemistry.chemical_element ,Substrate (chemistry) ,General Medicine ,Phosphate ,Analytical Chemistry ,chemistry.chemical_compound ,chemistry ,Response surface methodology ,Fourier transform infrared spectroscopy ,Mass fraction ,Food Science ,Plant Proteins - Abstract
The objective of this work is to obtain a new phosphate modified peanut protein (PMPP) with good physicochemical properties by using a simple and reliable method. For this purpose, response surface methodology (RSM), based on a Box-Behnken design, was used to optimise conditions for the microwave-assisted formation of phosphate modified peanut protein isolate (PPI) with sodium tripolyphosphate (STP) as the substrate. The results indicate that emulsification activity index (EAI) was optimised at 65.04 m(2)/g under the following conditions: substrate mass fraction of 10%, initial pH of 9.90, STP mass fraction of 7.70%, microwave power of 800 W, reaction temperature of 43.0 °C, and incubation time of 3.15 min. The emulsification activity of PPI improved after phosphate modification. Phosphatisation of both the Ser and Thr hydroxyl groups and the Lys amino groups was confirmed for the reaction between STP and PPI according to analysis of FTIR, XRD, TG/DTA, NMR-(31)P and SEM of the product. Physicochemical properties of PMPP were better than those of PPI.
- Published
- 2014
6. Ultrasonic-assisted enzymolysis to improve the antioxidant activities of peanut (Arachin conarachin L.) antioxidant hydrolysate
- Author
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Shaofang Liu, Lina Yu, Qingli Yang, Jie Bi, Jie Sun, and Chushu Zhang
- Subjects
Antioxidant ,Hot Temperature ,Arachis ,DPPH ,medicine.medical_treatment ,response-surface optimization ,Catalysis ,Hydrolysate ,Antioxidants ,Article ,Inorganic Chemistry ,chemistry.chemical_compound ,Hydrolysis ,antioxidant activities ,Ultrasonic assisted ,medicine ,Food science ,Response surface methodology ,Subtilisins ,Physical and Theoretical Chemistry ,Molecular Biology ,Spectroscopy ,Free Radical Scavenging Activity ,Protease ,peanut antioxidant hydrolysate ,ultrasonic-assisted enzymolysis ,Chemistry ,Organic Chemistry ,food and beverages ,General Medicine ,Hydrogen-Ion Concentration ,Computer Science Applications ,Sound ,Biochemistry - Abstract
The objective of this work is to provide a theoretical basis for preparing peanut antioxidant hydrolysate in order to improve its antioxidant activities. Therefore, response surface methodology (RSM) based on the Box-Behnken design was used to optimize ultrasonic-assisted enzymolysis for the purpose of preparing peanut antioxidant hydrolysate. Results indicated that the DPPH free radical scavenging activity of peanut hydrolysate could reach 90.06% under the following optimum conditions: ultrasonic power of 150.0 w, reaction temperature of 62.0 °C, incubation time of 25.0 min, and initial pH value of 8.5. The DPPH free radical scavenging rate of peanut hydrolysate from ultrasonic-assisted enzymolysis improved comparing with that of peanut hydrolysate from protease hydrolysis alone. The peanut antioxidant hydrolysate was found to display eight improved kinds of antioxidant activities. In conclusion, the optimal ultrasonic-assisted enzymolysis technology conditions described in this paper, appear to be beneficial for preparing peanut antioxidant hydrolysate.
- Published
- 2012
7. Antioxidant activity and phenolic compounds of Holotrichia parallela Motschulsky extracts
- Author
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Jie Bi, Lina Yu, Feng Zhu, Chushu Zhang, Jie Sun, Qingli Yang, Mingjing Qu, and Shaofang Liu
- Subjects
China ,Antioxidant ,Traditional medicine ,Chemistry ,Crop pest ,DPPH ,medicine.medical_treatment ,In vitro toxicology ,Catechin ,General Medicine ,Holotrichia parallela ,Antioxidants ,Analytical Chemistry ,Lipid peroxidation ,Coleoptera ,chemistry.chemical_compound ,Nutraceutical ,Biochemistry ,Phenols ,medicine ,Animals ,Medicine, Chinese Traditional ,Food Science - Abstract
Insects have been relatively unexplored as potential sources of natural antioxidants. We report the antioxidant activity of extracts of the adult large black chafer beetle Holotrichia parallela Motschulsky, a common crop pest in China. The antioxidant activity of the ethanolic extract (EE) and the water extract (WE) of adult H. parallela were evaluated by four different in vitro assays. EE showed potent metal-chelating activity and inhibition of lipid peroxidation. WE proved to be an excellent antioxidant in the scavenging of 2,2-diphenyl-1-picrylhydrazyl (DPPH) radicals and metal-chelating activity. Catechin was identified in the ethanolic extract and proteins were the main components in the water extracts. Both compounds could contribute to the antioxidant activity of the species. These results suggest that adult H. parallela might be used as a nutraceutical to alleviate oxidate-induced diseases and as a natural antioxidant additive in the food industry.
- Published
- 2011
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