24 results on '"Liang, Rui"'
Search Results
2. Size-dependent enhancement of gene expression by Plasmodium 5'UTR introns.
- Author
-
Lin, Lirong, Liu, Yanjing, Liang, Rui, Guo, Yue, Xu, Ruixue, Fan, Ruoxi, Jiao, Zhiwei, Zhao, Wenting, Yue, Lixia, Lu, Mingke, Liu, Shengfa, Su, Xin-zhuan, and Li, Jian
- Subjects
GENE expression ,PLASMODIUM ,INTRONS ,PLASMODIUM yoelii ,GENETIC regulation ,NUCLEOTIDE sequence - Abstract
Background: Eukaryotic genes contain introns that are removed by the spliceosomal machinery during mRNA maturation. Introns impose a huge energetic burden on a cell; therefore, they must play an essential role in maintaining genome stability and/or regulating gene expression. Many genes (> 50%) in Plasmodium parasites contain predicted introns, including introns in 5′ and 3′ untranslated regions (UTR). However, the roles of UTR introns in the gene expression of malaria parasites remain unknown. Methods: In this study, an episomal dual-luciferase assay was developed to evaluate gene expression driven by promoters with or without a 5′UTR intron from four Plasmodium yoelii genes. To investigate the effect of the 5′UTR intron on endogenous gene expression, the pytctp gene was tagged with 3xHA at the N-terminal of the coding region, and parasites with or without the 5′UTR intron were generated using the CRISPR/Cas9 system. Results: We showed that promoters with 5′UTR introns had higher activities in driving gene expression than those without 5′UTR introns. The results were confirmed in recombinant parasites expressing an HA-tagged gene (pytctp) driven by promoter with or without 5′UTR intron. The enhancement of gene expression was intron size dependent, but not the DNA sequence, e.g. the longer the intron, the higher levels of expression. Similar results were observed when a promoter from one strain of P. yoelii was introduced into different parasite strains. Finally, the 5′UTR introns were alternatively spliced in different parasite development stages, suggesting an active mechanism employed by the parasites to regulate gene expression in various developmental stages. Conclusions: Plasmodium 5′UTR introns enhance gene expression in a size-dependent manner; the presence of alternatively spliced mRNAs in different parasite developmental stages suggests that alternative slicing of 5′UTR introns is one of the key mechanisms in regulating parasite gene expression and differentiation. [ABSTRACT FROM AUTHOR]
- Published
- 2024
- Full Text
- View/download PDF
3. Growth Axis Somatostatin , Growth Hormone Receptor , and Insulin-like Growth Factor-1 Genes Express and Are Affected by the Injection of Exogenous Growth Hormone in Chinemys reevesii.
- Author
-
Xie, Rui-Lin, Liang, Rui, Luo, Yuan-Yuan, Ruan, Zhuo-Hao, Li, Yi-Fu, and Liu, Wen-Sheng
- Subjects
- *
SOMATOTROPIN , *HUMAN growth hormone , *PITUITARY dwarfism , *GENE expression , *SOMATOSTATIN , *SOMATOTROPIN receptors , *REGULATOR genes - Abstract
In this study, to explore the effect of growth hormone changes on the related genes and regulatory roles of the turtle, PCR amplification, real-time fluorescence quantitative analysis, and enzyme cutting technology were used to clone and sequence the somatostatin (SS) gene, growth hormone receptor (GHR), and insulin-like growth factor-1 (IGF-I) sequence of Chinemys reevesii. The effects of human growth hormone on the mRNA expression of growth-axis-related genes SS, GHR, and IGF-1 in different sexes were observed. The study of the SS gene in turtles using real-time fluorescence quantitative PCR showed that the SS gene was mainly expressed in the nervous system and the digestive system, with the highest expression found in the brain, while the GHR gene and the IGF-I gene were expressed in all tissues of Chinemys reevesii. The SS gene was expressed in the brain, pituitary, liver, stomach, and intestine, with the highest expression in the brain and the lowest expression in the liver. Within 4 weeks of the injection of exogenous growth hormone, the expression level of the SS gene in the brain of both sexes first increased and then decreased, showing a parabolic trend, and the expression level of the experimental group was lower than that of the control group. After the injection of growth hormone (GH), the expression of the GHR gene in the liver of both sexes showed a significant increase in the first week, decreasing to the control group level in the second week, and then gradually increasing. Finally, a significant level of difference in the expression of the GHR gene was reached at 3 and 4 weeks. In terms of the IGF-I gene, the changing trend of the expression level in the liver was the same as that of the GHR gene. After the injection of exogenous growth hormone, although the expression of the SS gene increased the inhibition of the secretion of the GHR gene by the Reeves' turtle, exogenous growth hormone could replace the synthesis of GH and GHR, accelerating the growth of the turtle. The experiments showed that the injection of recombinant human growth hormone affects the expression of SS, GHR, and IGF-1 genes, and promotes the growth of the Reeves' turtle. [ABSTRACT FROM AUTHOR]
- Published
- 2023
- Full Text
- View/download PDF
4. Establishment and verification of prognostic model and ceRNA network analysis for colorectal cancer liver metastasis.
- Author
-
Zhang, Xuan, Wu, Tao, Zhou, Jinmei, Chen, Xiaoqiong, Dong, Chao, Guo, Zhangyou, Yang, Renfang, Liang, Rui, Feng, Qing, Hu, Ruixi, Li, Yunfeng, and Ding, Rong
- Subjects
COLORECTAL liver metastasis ,PROGNOSTIC models ,DISEASE risk factors ,LINCRNA ,GENE expression ,ENDOMETRIUM ,GENE regulatory networks ,IMMUNOCOMPUTERS - Abstract
Objects: Colorectal cancer (CRC) is one of the most common cancers in the world. Approximately two-thirds of patients with CRC will develop colorectal cancer liver metastases (CRLM) at some point in time. In this study, we aimed to construct a prognostic model of CRLM and its competing endogenous RNA (ceRNA) network. Methods: RNA-seq of CRC, CRLM and normal samples were obtained from The Cancer Genome Atlas (TCGA) and Gene Expression Omnibus database. Limma was used to obtain differential expression genes (DEGs) between CRLM and CRC from sequencing data and GSE22834, and Gene Ontology and Kyoto Encyclopedia of Genes and Genomes functional enrichment analyses were performed, respectively. Univariate Cox regression analysis and lasso Cox regression models were performed to screen prognostic gene features and construct prognostic models. Functional enrichment, estimation of stromal and immune cells in malignant tumor tissues using expression data (ESTIMATE) algorithm, single-sample gene set enrichment analysis, and ceRNA network construction were applied to explore potential mechanisms. Results: An 8-gene prognostic model was constructed by screening 112 DEGs from TCGA and GSE22834. CRC patients in the TCGA and GSE29621 cohorts were stratified into either a high-risk group or a low-risk group. Patients with CRC in the high-risk group had a significantly poorer prognosis compared to in the low-risk group. The risk score was identified as an independent predictor of prognosis. Functional analysis revealed that the risk score was closly correlated with various immune cells and immune-related signaling pathways. And a prognostic gene-associated ceRNA network was constructed that obtained 3 prognosis gene, 14 microRNAs (miRNAs) and 7 long noncoding RNAs (lncRNAs). Conclusions: In conclusion, a prognostic model for CRLM identification was proposed, which could independently identify high-risk patients with low survival, suggesting a relationship between local immune status and prognosis of CRLM. Moreover, the key prognostic genes-related ceRNA network were established for the CRC investigation. Based on the differentially expressed genes between CRLM and CRC, the prognosis model of CRC patients was constructed. [ABSTRACT FROM AUTHOR]
- Published
- 2023
- Full Text
- View/download PDF
5. Targeting FTO suppresses hepatocellular carcinoma by inhibiting ERBB3 and TUBB4A expression.
- Author
-
Jiang, Lingli, Liang, Rui, Luo, Qing, Chen, Zhe, and Song, Guanbin
- Subjects
- *
GENE expression , *HEPATOCELLULAR carcinoma , *CELL migration , *PROTEIN-tyrosine kinases , *ADIPOSE tissues , *FAT - Abstract
[Display omitted] Fat mass and obesity-associated protein (FTO) is an N6-methyladenosine (m6A) demethylase and plays critical oncogenic roles in multiple cancers. Here we show that FTO is an effective target in hepatocellular carcinoma (HCC). FTO is highly expressed in patients with HCC. Genetic depletion of Fto dramatically attenuated HCC progression in mice. Pharmacological inhibition of FTO by FB23/FB23-2 markedly suppressed the proliferation and migration of HCC cell lines in vitro and inhibited HCC tumorigenicity in xeno-transplanted mice. Mechanistically, FB23-2 suppressed the expression of Erb-b2 receptor tyrosine kinase 3 (ERBB3) and human tubulin beta class Iva (TUBB4A) by increasing the m6A level in these mRNA transcripts. The decrease in ERBB3 expression resulted in the inhibition of Akt-mTOR signaling, which subsequently impaired the proliferation and survival of HCC cells. Moreover, FB23-2 disturbed the stability of the tubulin cytoskeleton, whereas overexpression of TUBB4A rescued the migration of HCC cells. Collectively, our study demonstrates that FTO plays a critical role in HCC by maintaining the proliferation and migration of cells and highlights the potential of FTO inhibitors for targeting HCC. [ABSTRACT FROM AUTHOR]
- Published
- 2024
- Full Text
- View/download PDF
6. Tetramethylpyrazine Inhibits the Proliferation and Invasion of Glioma Cells by Regulating the UBL7-AS1/miR-144-3p Pathway.
- Author
-
Liang, Rui, Zhang, Guofeng, Xu, Wenhua, Liu, Weibing, and Tang, Youjia
- Subjects
- *
RNA physiology , *HETEROCYCLIC compounds , *CANCER invasiveness , *GLIOMAS , *ANTINEOPLASTIC agents , *RNA , *CELLULAR signal transduction , *GENE expression , *BIOINFORMATICS , *CELL proliferation , *CELL migration inhibition , *CELL lines , *NEUROGLIA , *POLYMERASE chain reaction , *BIOLOGICAL assay , *PHARMACODYNAMICS - Abstract
This work aims to investigate the effects of tetramethylpyrazine (TMP) on the proliferation, migration, and invasion of glioma cells and to analyze the regulation mechanism of TMP on the long noncoding RNA UBL7-AS1/miR-144-3p pathway. Glioma cell line and normal astrocytes were collected. The expression of UBL7-AS1 was detected by real-time PCR. The glioma cells were overexpressed with UBL7-AS1. CCK-8 and Transwell assays were used to detect cell proliferation and cell invasion ability, respectively. Bioinformatics was adopted to predict the possible regulatory mechanisms of UBL7-AS1. The dual luciferase reporter gene was applied to verify the regulatory effect of RNA UBL7-AS1 with miR-144-3p. TMP inhibited the proliferation and invasion of glioma cells. UBL7-AS1 was highly expressed in glioma tissues and cells. The overexpression of UBL7-AS1 promotes the cell proliferation and invasion of glioma. UBL7-AS1 can act as a sponge for miR-144-3p in glioma cells. The overexpression of UBL7-AS1 can reverse the inhibition of TMP on proliferation, migration, and invasion of glioma cells. TMP inhibits the proliferation, migration, and invasion of glioma cells by regulating the UBL7-AS1/miR-144-3p pathway. [ABSTRACT FROM AUTHOR]
- Published
- 2022
- Full Text
- View/download PDF
7. Genome-wide association study on Northern Chinese identifies KLF2, DOT1L and STAB2 associated with systemic lupus erythematosus.
- Author
-
Song, Qin, Lei, Yao, Shao, Li, Li, Weiyang, Kong, Qingsheng, Lin, Zhiming, Qin, Xiao, Wei, Wei, Hou, Fei, Li, Jian, Guo, Xianghua, Mao, Yujing, Cao, Yujie, Liu, Zhongyi, Zheng, Lichuan, Liang, Rui, Jiang, Yuping, Liu, Yan, Zhang, Lili, and Yang, Jing
- Subjects
TELOMERES ,GENETICS ,CONFIDENCE intervals ,META-analysis ,SINGLE nucleotide polymorphisms ,RACE ,MACROPHAGES ,GENE expression ,GENOMES ,GENOTYPES ,DESCRIPTIVE statistics ,SYSTEMIC lupus erythematosus ,ODDS ratio ,DISEASE risk factors - Abstract
Objectives To identify novel genetic loci associated with systemic lupus erythematosus (SLE) and to evaluate potential genetic differences between ethnic Chinese and European populations in SLE susceptibility. Methods A new genome-wide association study (GWAS) was conducted from Jining, North China, on 1506 individuals (512 SLE cases and 994 matched healthy controls). The association results were meta-analysed with existing data on Chinese populations from Hong Kong, Guangzhou and Central China, as well as GWAS results from four cohorts of European ancestry. A total of 26 774 individuals (9310 SLE cases and 17 464 controls) were included in this study. Results Meta-analysis on four Chinese cohorts identifies KLF2 as a novel locus associated with SLE [rs2362475; odds ratio (OR) = 0.85, P =2.00E-09]. KLF2 is likely an Asian-specific locus as no evidence of association was detected in the four European cohorts (OR = 0.98, P =0.58), with evidence of heterogeneity (P =0.0019) between the two ancestral groups. Meta-analyses of results from both Chinese and Europeans identify STAB2 (rs10082873; OR= 0.89, P =4.08E-08) and DOT1L (rs4807205; OR= 1.12, P =8.17E-09) as trans-ancestral association loci, surpassing the genome-wide significance. Conclusions We identified three loci associated with SLE, with KLF2 a likely Chinese-specific locus, highlighting the importance of studying diverse populations in SLE genetics. We hypothesize that DOT1L and KLF2 are plausible SLE treatment targets, with inhibitors of DOT1L and inducers of KLF2 already available clinically. [ABSTRACT FROM AUTHOR]
- Published
- 2021
- Full Text
- View/download PDF
8. Increased frequency of β cells with abnormal NKX6.1 expression in type 2 diabetes but not in subjects with higher risk for type 2 diabetes.
- Author
-
Liu, Tengli, Sun, Peng, Zou, Jiaqi, Wang, Le, Wang, Guanqiao, Liu, Na, Liu, Yaojuan, Ding, Xuejie, Zhang, Boya, Liang, Rui, Wang, Shusen, and Shen, Zhongyang
- Subjects
GLYCOSYLATED hemoglobin ,PANCREAS ,HYPERGLYCEMIA ,AGE distribution ,CELL physiology ,ISLANDS of Langerhans ,GENE expression ,TYPE 2 diabetes ,FLUORESCENT antibody technique ,TRANSCRIPTION factors - Abstract
Background: NKX6.1 is a transcription factor for insulin, as well as a marker for β cell maturity. Abnormal NKX6.1 expression in β cells, such as translocation from the nucleus to cytoplasm or lost expression, has been shown as a marker for β cell dedifferentiation. Methods: We obtained pancreatic sections from organ donors and immunofluorescence staining with NKX6.1 and insulin was performed to characterize NKX6.1 expression in subjects with or without type 2 diabetes mellitus (T2DM). Results: Our results showed that cells with insulin expression but no nucleic NKX6.1 expression (NKX6.1
Nuc- Ins+ ), and cells with cytoplasmic NKX6.1 expression but no insulin expression (NKX6.1cyt Ins− ) were significantly increased in T2DM subjects and positively correlated with glycated hemoglobin (HbA1c), indicating the elevated β cell dedifferentiation with NKX6.1 inactivation in T2DM. To investigate whether β cell dedifferentiation has initiated in subjects with higher risks for T2DM, we next analyzed the association between β-cell dedifferentiation level in ND subjects with different ages, body mass index, and HbA1c. The results showed the absolute number and percentage of dedifferentiated β cells with NKX6.1 inactivation did not significantly change in subjects with advanced aging, obesity, or modest hyperglycemia, indicating that the β cell dedifferentiation might mainly occur after T2DM was diagnosed. Conclusion: Our results suggested that NKX6.1 expression in β cells was changed in type 2 diabetic subjects, evidenced by significantly increased NKX6.1Nuc- Ins+ and NKX6.1cyt Ins− cells. This abnormality did not occur more frequently in subjects with a higher risk for T2DM, suggesting that β cell dedifferentiation might be secondary to the pathological changes in T2DM. [ABSTRACT FROM AUTHOR]- Published
- 2021
- Full Text
- View/download PDF
9. Identification of the cDNA Encoding the Growth Hormone Receptor (GHR) and the Regulation of GHR and IGF-I Gene Expression by Nutritional Status in Reeves' Turtle (Chinemys reevesii).
- Author
-
Zhu, Wenlu, He, Yuhui, Ruan, Zhuohao, Zhang, Xiquan, Liao, Liangyuan, Gao, Yicong, Lin, Nani, Chen, Xiancan, Liang, Rui, and Liu, Wen-sheng
- Subjects
SOMATOTROPIN receptors ,GONADS ,SOMATOMEDIN C ,ANTISENSE DNA ,GENE expression ,NUTRITIONAL status ,NUTRITIONAL genomics - Abstract
Chinemys reevesii (Reeves' turtle) is a slow-growing reptile that is distributed widely across China. Prior to this study, the cDNA sequence of the growth hormone receptor (GHR) in the Reeve's turtle, or how periods of starvation might influence the gene expression of GHR and insulin-like growth factor I (IGF-I) in this species, were unknown. Here, we identified the full-length sequence of the cDNA encoding GHR in Reeves' turtle by using RT-PCR and RACE. The full-length GHR cDNA was identified to be 3936 base-pairs in length, with a 1848 base-pair open reading frame (ORF) that encodes a 615 amino acid protein. Analysis showed that GHR mRNA was detectable in a wide range of tissues; the highest and lowest levels of expression were detected in the liver and the gonad, respectively. IGF-I was also expressed in a range of tissues, but not in the gonad; the highest levels of IGF-I expression were detected in the liver. After 4 weeks of fasting, the expression levels of GHR and IGF-I in the liver had decreased significantly; however, these gradually returned to normal after refeeding. We report the first cloned cDNA sequence for the GHR gene in the Reeve's turtle. Our findings provide a foundation from which to investigate the specific function of the GHR in Reeve's turtle, and serve as a reference for studying the effects of different nutrient levels on GHR expression in this species. [ABSTRACT FROM AUTHOR]
- Published
- 2020
- Full Text
- View/download PDF
10. Hirsutella sinensis Attenuates Aristolochic Acid-Induced Renal Tubular Epithelial-Mesenchymal Transition by Inhibiting TGF-β1 and Snail Expression
- Author
-
Xiao-Yi Xu, Yi-pu Chen, Yan-yan Wang, Yu-lin Man, Jing-jing Chai, Hong-liang Rui, Hong-rui Dong, and Hong Cheng
- Subjects
Male ,0301 basic medicine ,Physiology ,Protein Expression ,Hirsutella ,lcsh:Medicine ,Artificial Gene Amplification and Extension ,Snail ,Biochemistry ,Polymerase Chain Reaction ,Epithelium ,Keratin 18 ,chemistry.chemical_compound ,Animal Cells ,Medicine and Health Sciences ,Small interfering RNAs ,RNA, Small Interfering ,lcsh:Science ,Cells, Cultured ,Multidisciplinary ,biology ,Transfection ,Nucleic acids ,Kidney Tubules ,Aristolochic Acids ,Kidney Diseases ,Cellular Types ,Anatomy ,Research Article ,Epithelial-Mesenchymal Transition ,Kidney Cortex ,Excretion ,Aristolochic acid ,Research and Analysis Methods ,Cell Line ,Transforming Growth Factor beta1 ,03 medical and health sciences ,Ascomycota ,biology.animal ,Botany ,Gene Expression and Vector Techniques ,Genetics ,Animals ,Humans ,RNA, Messenger ,Epithelial–mesenchymal transition ,Molecular Biology Techniques ,Non-coding RNA ,Molecular Biology ,Molecular Biology Assays and Analysis Techniques ,Keratin-18 ,lcsh:R ,Biology and Life Sciences ,Epithelial Cells ,Kidneys ,Cell Biology ,Renal System ,biology.organism_classification ,Fibrosis ,Molecular biology ,Actins ,Gene regulation ,Rats ,Disease Models, Animal ,Biological Tissue ,030104 developmental biology ,Gene Expression Regulation ,chemistry ,Cell culture ,RNA ,lcsh:Q ,Gene expression ,Snail Family Transcription Factors ,Physiological Processes ,Developmental Biology ,Transcription Factors ,Transforming growth factor - Abstract
Objective To investigate the inhibitory effect of Hirsutella sinensis (HS) on epithelial-mesenchymal transition (EMT) of renal tubular epithelial cells induced by aristolochic acid (AA) and its possible mechanism. Methods 18 male Sprague-Dawley rats were randomly and equally divided into the following 3 groups: AA group, AA+HS group and control group. Urinary protein excretion and creatinine clearance (CCr) were measured. All rats were sacrificed at the end of 12th week. The pathological examination of renal tissue was performed and the mRNA and protein expression of transforming growth factor-β1 (TGF-β1), α-smooth muscle actin (α-SMA), cytokeratin-18 and Snail in renal cortex were determined by real time quantitative PCR and immunohistochemical staining respectively. In addition, human renal proximal tubule epithelial cells line (HKC) was divided into the following 4 groups: AA group, AA+HS group, HS control group and control group. The above mRNA and protein expression in HKC was determined by real time quantitative PCR and Western blot respectively. Results (1) CCr was significantly decreased, and the urinary protein excretion and relative area of renal interstitial fibrosis were significantly increased in the rats of AA and AA+HS group compared to those in control group (P
- Published
- 2016
11. H19X-encoded miR-424(322)/-503 cluster: emerging roles in cell differentiation, proliferation, plasticity and metabolism.
- Author
-
Wang, Fan, Liang, Rui, Tandon, Neha, Matthews, Elizabeth R., Shrestha, Shreesti, Yang, Jiao, Soibam, Benjamin, Yang, Jin, and Liu, Yu
- Subjects
- *
MICRORNA , *CELL differentiation , *CELL proliferation , *CELL metabolism , *GENE expression - Abstract
miR-424(322)/-503 are mammal-specific members of the extended miR-15/107 microRNA family. They form a co-expression network with the imprinted lncRNA H19 in tetrapods. miR-424(322)/-503 regulate fundamental cellular processes including cell cycle, epithelial-to-mesenchymal transition, hypoxia and other stress response. They control tissue differentiation (cardiomyocyte, skeletal muscle, monocyte) and remodeling (mammary gland involution), and paradoxically participate in tumor initiation and progression. Expression of miR-424(322)/-503 is governed by unique mechanisms involving sex hormones. Here, we summarize current literature and provide a primer for future endeavors. [ABSTRACT FROM AUTHOR]
- Published
- 2019
- Full Text
- View/download PDF
12. 5-aza-2'-deoxycytidine leads to reduced embryo implantation and reduced expression of DNA methyltransferases and essential endometrial genes
- Author
-
Junlin He, Yinyin Xia, Xuemei Chen, Xueqing Liu, Yubin Ding, Yingxiong Wang, Liang-Rui Guo, and Chunlan Long
- Subjects
Male ,Methyltransferase ,Anatomy and Physiology ,Mouse ,Estrogen receptor ,lcsh:Medicine ,Biochemistry ,DNA Methyltransferase 3A ,chemistry.chemical_compound ,Endometrium ,Mice ,Endocrinology ,Pregnancy ,Reproductive Physiology ,Nucleic Acids ,DNA (Cytosine-5-)-Methyltransferases ,lcsh:Science ,Multidisciplinary ,Animal Models ,Vitamins ,Immunohistochemistry ,DNA methylation ,embryonic structures ,Azacitidine ,Medicine ,Female ,Epigenetics ,Receptors, Progesterone ,DNA modification ,Research Article ,DNA (Cytosine-5-)-Methyltransferase 1 ,Blotting, Western ,chemical and pharmacologic phenomena ,Biology ,Decitabine ,Model Organisms ,Progesterone receptor ,mental disorders ,Genetics ,Animals ,Embryo Implantation ,Gene ,Nutrition ,Homeodomain Proteins ,lcsh:R ,Estrogen Receptor alpha ,Reproductive System ,DNA ,DNA Methylation ,Molecular biology ,Demethylating agent ,Homeobox A10 Proteins ,chemistry ,lcsh:Q ,Gene expression ,Estrogen receptor alpha - Abstract
BACKGROUND: The DNA demethylating agent 5-aza-2'-deoxycytidine (5-aza-CdR) incorporates into DNA and decreases DNA methylation, sparking interest in its use as a potential therapeutic agent. We aimed to determine the effects of maternal 5-aza-CdR treatment on embryo implantation in the mouse and to evaluate whether these effects are associated with decreased levels of DNA methyltransferases (Dnmts) and three genes (estrogen receptor α [Esr1], progesterone receptor [Pgr], and homeobox A10 [Hoxa10]) that are vital for control of endometrial changes during implantation. METHODS AND PRINCIPAL FINDINGS: Mice treated with 5-aza-CdR had a dose-dependent decrease in number of implantation sites, with defected endometrial decidualization and stromal cell proliferation. Western blot analysis on pseudo-pregnant day 3 (PD3) showed that 0.1 mg/kg 5-aza-CdR significantly repressed Dnmt3a protein level, and 0.5 mg/kg 5-aza-CdR significantly repressed Dnmt1, Dnmt3a, and Dnmt3b protein levels in the endometrium. On PD5, mice showed significantly decreased Dnmt3a protein level with 0.1 mg/kg 5-aza-CdR, and significantly decreased Dnmt1 and Dnmt3a with 0.5 mg/kg 5-aza-CdR. Immunohistochemical staining showed that 5-aza-CdR repressed DNMT expression in a cell type-specific fashion within the uterus, including decreased expression of Dnmt1 in luminal and/or glandular epithelium and of Dnmt3a and Dnmt3b in stroma. Furthermore, the 5' flanking regions of the Esr1, Pgr, and Hoxa10 were hypomethylated on PD5. Interestingly, the higher (0.5 mg/kg) dose of 5-aza-CdR decreased protein expression of Esr1, Pgr, and Hoxa10 in the endometrium on PD5 in both methylation-dependent and methylation-independent manners. CONCLUSIONS: The effects of 5-aza-CdR on embryo implantation in mice were associated with altered expression of endometrial Dnmts and genes controlling endometrial changes, suggesting that altered gene methylation, and not cytotoxicity alone, contributes to implantation defects induced by 5-aza-CdR.
- Published
- 2011
13. [Protective effects of Hirsutella sinensis on renal interstitial fibrosis: experiment with rat model of chronic aristolochic acid nephropathy]
- Author
-
Yun-feng, Zhu, Yi-Pu, Chen, Hong-liang, Rui, Hong-rui, Dong, and Zhao, Hu
- Subjects
Male ,Biological Products ,Tissue Inhibitor of Metalloproteinase-1 ,Reverse Transcriptase Polymerase Chain Reaction ,Connective Tissue Growth Factor ,Gene Expression ,Kidney ,Fibrosis ,Immunohistochemistry ,Collagen Type I ,Immediate-Early Proteins ,Rats ,Rats, Sprague-Dawley ,Disease Models, Animal ,Random Allocation ,Transforming Growth Factor beta ,Chronic Disease ,Hypocreales ,Plasminogen Activator Inhibitor 1 ,Animals ,Aristolochic Acids ,Intercellular Signaling Peptides and Proteins ,Nephritis, Interstitial ,RNA, Messenger - Abstract
To study the protective effects of Hirsutella sinensis on renal interstitial fibrosis in chronic aristolochic acid nephropathy (CAAN).Eighteen male SD rats were divided into 3 equal groups: model group, given the extract of Aristolochia manshuriensis Kom (AmK) by gavage in the morning everyday for 12 weeks, intervention group, given the extract of Amk in the morning and suspension of Hirsutella sinensis in the afternoon by gavage once a day for 12 weeks, and control group, receiving tap water only by gavage. Bodyweight, urinary glucose, 24 h urinary protein excretion, and serum creatinine (SCr) were measured at the ends of the 1st, 4th, 8th, and 12th weeks respectively. At the end of the 12th week, all the rats were sacrificed with their kidneys taken out to undergo pathological examination. RT-PCR and immunohistochemistry were used to detect the mRNA and protein expression of transforming growth factor-beta1 (TGF-beta1), connective tissue growth factor (CTGF), plasminogen activator inhibitor-1 (PAI-1), tissue inhibitor of metalloproteinase-1 (TIMP-1), and type I collagen (ColI) in the kidney tissues.Since the 1st week, the urinary protein excretion and SCr levels in the model group were significantly higher than those in the control group (P0.01 or 0.05). At the end of the 12th week, the relative area of interstitial fibrosis of the model group was significantly enlarged (P0.01). The mRNA expression levels of TGF-beta1, CTGF, PAI-1, TIMP-1, and ColI in the model group were up-regulated by 4.19, 2.66, 6.12, 3.09, and 7.03 times respectively, and their protein expression levels were up-regulated by 2.31, 3.53, 3.17, 3.18, and 6.87 times respectively (all P0.01). By the end of the 12th week, the urinary protein excretion, SCr level and the relative area of interstitial fibrosis in the intervention group were all significantly lower than those in the model group (all P0.05). The mRNA expression levels of TGF-beta, CTGF, PAI-1, TIMP-1, and ColIof the intervention group were all significantly lower than those of the model group (all P0.05) with the inhibition rates of 45%, 41%, 47%, 48%, and the protein expression levels of TGF-beta, CTGF, PAI-1, TIMP-1, and ColI of the intervention group were all significantly lower than those of the model group (all P0.05) with the inhibition rates of 38%, 39%, 49%, 46%, and 61% respectively.Hirsutella sinensis can inhibit the production of TGF-beta1 and CTGF, factors that promote the extracellular matrix (ECM) synthesis and TIMP-1 and PAI-1, factors that antagonize ECM degradation in kidney tissues, thus alleviating renal interstitial fibrosis and improving renal function in CAAN.
- Published
- 2008
14. DNMT1 cooperates with MBD4 to inhibit the expression of Glucocorticoid-induced TNFR-related protein in human T cells.
- Author
-
Wang, Shuaiwei, Li, Yangyang, Zhu, Fangming, Lin, Fang, Luo, Xuerui, Zhao, Binbin, Zhang, Peng, Li, Dan, Gao, Yayi, Liang, Rui, Liu, Luyan, Tsun, Andy, Yuan, Xiaojun, Wu, Kejin, and Li, Bin
- Subjects
GLUCOCORTICOIDS ,TUMOR necrosis factor receptors ,GENE expression ,PROMOTERS ,CD25 antigen - Abstract
Glucocorticoid-induced TNFR-related protein ( GITR) is constitutively expressed in T regulatory (Treg) cells and regulates their suppressive function. We identified two methylated CpG islands in the Gitr locus. Using a Ch IP assay, we demonstrate that both DNMT1 and methyl-CpG-binding domain Protein 4 ( MBD4) bind to the Gitr promoter. Moreover, knockdown of DNMT1 decreases the binding activity of MBD4. We observed much higher levels of both DNMT1 and MBD4 in human CD4
+ CD25− conventional T (Tconv) cells. Moreover, co-overexpression of DNMT1 and MBD4 in Treg cells significantly inhibits GITR expression and impairs their suppressive activity. Our results reveal a novel molecular mechanism by which MBD4 inhibits GITR expression in a DNMT1-dependent manner. [ABSTRACT FROM AUTHOR]- Published
- 2017
- Full Text
- View/download PDF
15. An improved method with high sensitivity and low background in detecting low β-galactosidase expression in mouse embryos.
- Author
-
Shen, Xiaopeng, Bao, Wenjing, Yu, Wei, Liang, Rui, Nguyen, Bao, and Liu, Yu
- Subjects
GALACTOSIDASES ,PROTEIN expression ,REPORTER genes ,LABORATORY mice ,DEVELOPMENTAL biology - Abstract
LacZ is widely used as a reporter in studies of gene expression patterns. β-galactosidase, the product of LacZ gene, is usually detected by X-gal/FeCN staining. In X-gal/FeCN staining, β-galactosidase catalyzes X-gal to produce blue precipitates, which indicate the expression patterns of the gene of interest. A newer LacZ detection method using S-gal/TNBT is more sensitive but plagued by high background. Here, we describe an improved procedure that combines advantageous steps from the two methods. By comparing with X-gal/FeCN and S-gal/TNBT methods in detecting the expression patterns of miR-322/503 and miR-451 at a series of developmental stages, the improved method showed higher sensitivity and lower background. Thus, the improved method could be an alternative way of β-galactosidase staining in low gene expression situations. [ABSTRACT FROM AUTHOR]
- Published
- 2017
- Full Text
- View/download PDF
16. Recombinant Nogo-66 via soluble expression with SUMO fusion in Escherichia coli inhibits neurite outgrowth in vitro.
- Author
-
Dai, Xiaoyong, Sun, Zhongqing, Liang, Rui, Li, Yu, Luo, Huanmin, Huang, Yadong, Chen, Meiwan, Su, Zhijian, and Xiao, Fei
- Subjects
GENE expression ,RECOMBINANT proteins ,SMALL ubiquitin-related modifier proteins ,NOGO protein ,HYDROPHOBIC surfaces ,AFFINITY chromatography ,ELECTROSPRAY ionization mass spectrometry ,ESCHERICHIA coli - Abstract
Nogo-66, a hydrophilic loop of 66 amino acids flank two hydrophobic domains of the Nogo-A C terminus, interacts with the Nogo-66 receptor (NgR) to exert numerous functions in the central nervous system (CNS). Nogo-66 has important roles in aspects of neuronal development, including cell migration, axon guidance, fasciculation, and dendritic branching, and in aspects of CNS plasticity, including oligodendrocyte differentiation and myelination. Here, the small ubiquitin-related modifier (SUMO) was fused to the target gene, Nogo-66, and the construct was expressed in Escherichia coli ( E. coli). Under the optimal fermentation conditions, the soluble expression level of the fusion protein was 33 % of the total supernatant protein. After cleaving the fusion proteins with SUMO protease and purifying them by Ni-NTA affinity chromatography, the yield and purity of recombinant Nogo-66 obtained by 10-L scale fermentation were 23 ± 1.5 mg/L and greater than 93 %, respectively. The authenticity of the recombinant Nogo-66 was confirmed by an electrospray ionization-mass spectrometry analysis. The functional analyses indicated that the recombinant Nogo-66 was capable of binding the NgR specifically. The immunofluorescence results showed that the recombinant Nogo-66 could significantly inhibit neurite outgrowth of rat pheochromocytoma (PC12) cells stimulated by nerve growth factor and cerebellar granule cells (CGCs). Furthermore, Nogo-66 inhibited neurite outgrowth by increasing the level of phosphorylated Rho-associated coiled-coil-containing protein kinase 2 (ROCK2), collapsin response mediator protein 2 (CRMP2), and myosin light chain (MLC). This study provided a feasible and convenient production method for generating sufficient recombinant Nogo-66 for experimental and clinical applications. [ABSTRACT FROM AUTHOR]
- Published
- 2015
- Full Text
- View/download PDF
17. Suppression of Graft Regeneration, Not Ischemia/Reperfusion Injury, Is the Primary Cause of Small-for-Size Syndrome after Partial Liver Transplantation in Mice.
- Author
-
Pan, Ning, Lv, Xiangwei, Liang, Rui, Wang, Liming, and Liu, Qinlong
- Subjects
IMMUNOSUPPRESSION ,GRAFT rejection ,ISCHEMIA ,REPERFUSION injury ,LIVER transplantation ,LABORATORY mice ,GENE expression - Abstract
Background: Ischemia/reperfusion injury (IRI) is commonly considered to play a crucial role in the pathogenesis of small-for-size syndrome (SFSS) after liver transplantation. Rapid regeneration is also considered essential for the survival of SFS grafts. Methods: Mouse models of full-size orthotopic liver transplantation, 50% partial liver transplantation and 30% partial liver transplantation were established. Survival rate and serum alanine aminotransferase were observed. IRI was assessed by hepatic pathologic alterations, apoptosis and necrosis. Regeneration response was detected by mitotic index, BrdU incorporation and PCNA, Cyclin D1 and Cyclin E expression. The expression of mTOR, AKT, ERK, JNK2 and p70S6K, also involved in regeneration signaling pathways, were analyzed as well. Results: 30% partial liver graft resulted in a significantly low 7-day survival rate (P = 0.002) with no marked difference in tissue injury compared with the 50% partial graft group. Serum alanine aminotransferase levels were not significantly different between partial transplantation and full-size transplantation. Western blot analysis of caspase-3 and TUNEL staining also indicated no significant difference in apoptosis response between 30% partial transplantation and half-size or full-size transplantation (P = 0.436, P = 0.113, respectively). However, liver regeneration response indicators, mitotic index (P<0.0001) and BrdU (P = 0.0022), were markedly lower in 30% LTx compared with 50% LTx. Suppressed expression of PCNA, cyclin D1, cyclin E, mTOR, JNK2, AKT, ERK and p70S6K was also detected by western blot. Conclusions: Liver regeneration is markedly suppressed in SFSS, and is more likely the primary cause of SFSS, rather than ischemia/reperfusion injury. Therapy for recovering graft regeneration could be a potentially important strategy to reduce the incidence of SFSS. [ABSTRACT FROM AUTHOR]
- Published
- 2014
- Full Text
- View/download PDF
18. Effect of three dental alloys on cytotoxicity and apoptosis related gene expression in L929 cells.
- Author
-
MENG He, LI Xiu-mei, XU Yan-li, LIANG Rui-ying, and WU Wen-hui
- Subjects
DENTAL metallurgy ,CELL-mediated cytotoxicity ,APOPTOSIS ,GENE expression ,LEACHING ,IN vitro studies ,MITOCHONDRIAL physiology - Abstract
PURPOSE: To investigate the effect of the liching liquids of 3 different kinds of dental alloys on L929 cells both at cell level and molecular level. METHODS: The fibroblast L929 cells of mouse were treated in vitro with leaching liquids of 3 different kinds of dental alloys which were Au alloy, Ti and Ni-Cr alloy, respectively. The RPMI 1640 cell medium containing 10% fetal calf serum was served as a negative control and the Cu alloy was served as a positive control. The cytotoxicities of 3 dental alloys were evaluated by means of MTT and the effects of these alloys on the expression of caspase-3, caspase-8 and caspase-9 of L929 cells were examined by RT-PCR method and immunohistochemistry. The data was analyzed with SPSS 13.0 software package. RESULTS: The cytotoxicity of three groups were all in grade 0 after 48 hours of cultures. Caspase-8 had no change in all groups at mRNA level. The expression of caspase-3 and caspase-9 in Ni-Gr alloy group was significantly different from other groups. CONCLUSIONS: It is suggested from the results that the leaching liquids of Ni-Cr alloy may induce cell apoptosis through mitochondrion pathway. [ABSTRACT FROM AUTHOR]
- Published
- 2013
19. Hedgehog signaling displays a biphasic expression pattern during intestinal injury and repair.
- Author
-
Liang, Rui, Morris, Peter, Cho, Steven S.C., Abud, Helen E., Jin, Xianqing, and Cheng, Wei
- Subjects
HEDGEHOG signaling proteins ,CELLULAR signal transduction ,GENE expression ,INTESTINAL injuries ,KNOCKOUT mice ,MITOSIS ,IMMUNOHISTOCHEMISTRY ,THERAPEUTICS - Abstract
Abstract: Background/Purpose: Gastrointestinal injury is common clinically. The exact mechanism by which gastrointestinal repair occurs has yet to be well defined. Hedgehog (Hh) signaling is known to be involved in gastrointestinal development and repair of tissues such as skin and heart. The present study aimed to investigate the role of Hh in the repair of the small intestine. Methods: i) To study acute intestinal injury, we optimized a mouse model of 5-flurouracil (5-FU) induced injury of the small intestine. Ileal tissues were evaluated for injury and repair markers at day 0, 2, 5, and 9. ii) Immunohistochemistry (Sonic hedgehog, Shh), in situ hybridization (Shh), and Ptch/LacZ transgenic mice were carried out to localize hedgehog expression. A33CrPr × ShhTg knock-in mice were bred to study the effect of Shh over-expression. qPCR of Shh, Ihh, Ptch, Bmp4 was carried out to quantify hedgehog signaling. iii) 5FU treated mice were then treated with a hedgehog inhibitor or saline (control) and the effects of Shh inhibition including apoptosis, proliferation, and mitosis were then compared. Results: i) Immunohistochemistry and in situ hybridization of Shh, qPCR of hedgehog signaling pathway genes, and Ptch/LacZ staining results consistently showed down-regulation during the injury phase (P<0.05) followed by up-regulation during the repair phase (P<0.005). ii) Hh signaling inhibition following 5-FU induced injury augmented apoptotic activity (P<0.05), suppressed mitotic activity (P<0.005) in intestinal crypts, and reduced Paneth cell hyperplasia (P<0.005). iii) Shh over-expression in conditionally knock-mice led to increased mitotic, Paneth, and goblet cells. Conclusion: Hedgehog signaling pathway displays a biphasic expression pattern during the injury/repair of small intestine. It may play an important regulatory role in intestinal repair. [Copyright &y& Elsevier]
- Published
- 2012
- Full Text
- View/download PDF
20. L2,1-Extreme Learning Machine: An Efficient Robust Classifier for Tumor Classification.
- Author
-
Ren, Liang-Rui, Gao, Ying-Lian, Liu, Jin-Xing, Zhu, Rong, and Kong, Xiang-Zhen
- Subjects
- *
MACHINE learning , *TUMOR classification , *RNA sequencing , *GENE expression , *IMAGE databases - Abstract
• Based on L 21 -norm, a robust Extreme Learning Machine method called L 21 -ELM is proposed. • Various benchmark datasets downloaded from the UCI database and some image datasets are used to train and test the model. • The proposed L 21 -ELM is applied to the classification of cancer samples and single-cell data. • The proposed method not only inherits the advantages of original ELM, such as easy implementation and fast speed, but also shows better generalization performance. With the development of cancer research, various gene expression datasets containing cancer information show an explosive growth trend. In addition, due to the continuous maturity of single-cell RNA sequencing (scRNA-seq) technology, the protein information and pedigree information of a single cell are also continuously mined. It is a technical problem of how to classify these high-dimensional data correctly. In recent years, Extreme Learning Machine (ELM) has been widely used in the field of supervised learning and unsupervised learning. However, the traditional ELM does not consider the robustness of the method. To improve the robustness of ELM, in this paper, a novel ELM method based on L 2 , 1 -norm named L 2 , 1 -Extreme Learning Machine (L 2 , 1 -ELM) has been proposed. The method introduces L 2 , 1 -norm on loss function to improve the robustness, and minimizes the influence of noise and outliers. Firstly, we evaluate the new method on five UCI datasets. The experiment results prove that our method can achieve competitive results. Next, the novel method is applied to the problem of classification of cancer samples and single-cell RNA sequencing datasets. The experimental results on The Cancer Genome Atlas (TCGA) datasets and scRNA-seq datasets prove that ELM and its variants has great potential in the classification of cancer samples. [ABSTRACT FROM AUTHOR]
- Published
- 2020
- Full Text
- View/download PDF
21. Visfatin promotes the malignancy of human acute myeloid leukemia cells via regulation of IL-17.
- Author
-
Hui, Zengqian, Liu, Zhao, He, Aili, Chen, Yinxia, Zhang, Pengyu, Lei, Bo, Yao, Huan, Yu, Yong, Liang, Rui, Li, Zhanning, and Zhang, Wanggang
- Subjects
- *
ACUTE myeloid leukemia treatment , *CANCER cells , *INTERLEUKIN-17 , *DOXORUBICIN , *GENE expression , *CISPLATIN - Abstract
Understanding the mechanisms of malignancy in acute myeloid leukemia (AML) cell is important for the targeted treatment and drug development. We found that visfatin, a 52-kDa adipokine, can positively regulate the proliferation of AML cells. Targeted inhibition of visfatin via its specific siRNAs or inhibitor can suppress the proliferation of AML cells. Further, knockdown of visfatin can increase the doxorubicin (Dox) and cisplatin (CDDP) sensitivity of AML cells. Among the tested six cytokines, si-visfatin can decrease the expression of interleukin-17 (IL-17). Over expression of IL-17 can reverse si-visfatin suppressed cell proliferation and increased Dox sensitivity. The upregulation of IL-17 was also involved in visfatin induced activation of PI3K/Akt signals in AML cells. The inhibitor of PI3K/Akt can synergistically suppress the proliferation of HL60 cells which were transfected with si-visfatin. Knockdown of visfatin can increase the expression of miR-135a, which can bind to the 3′UTR of IL-17 and decrease its expression. The inhibitor of miR-135a can attenuate si-visfatin suppressed expression of IL-17 and proliferation of AML cells. Collectively, our data suggested that visfatin can increase the malignancy of AML cells via regulation of miR-135a/IL-17/PI3K/Akt signals. [ABSTRACT FROM AUTHOR]
- Published
- 2019
- Full Text
- View/download PDF
22. Basic fibroblast growth factor promotes stem Leydig cell development and inhibits LH-stimulated androgen production by regulating microRNA expression.
- Author
-
Liu, Hui, Yang, Yan, Zhang, Lei, Liang, Rui, Ge, Ren-shan, Zhang, Yufei, Zhang, Qihao, Xiang, Qi, Huang, Yadong, and Su, Zhijian
- Subjects
- *
FIBROBLAST growth factors , *LEYDIG cells , *LUTEINIZING hormone , *ANDROGENS , *MICRORNA , *GENE expression - Abstract
Leydig cells are the primary source of testosterone in the testes, and their steroidogenic function is strictly controlled by the hypothalamus–pituitary–gonad axis. Emerging evidence has indicated that fibroblast growth factors play a role in regulating stem Leydig cell development and steroidogenesis, but little is known about the regulatory mechanism. Using a seminiferous tubule culture system, we demonstrated that basic fibroblast growth factor (bFGF) can promote stem Leydig cell proliferation and commitment toward differentiation in testosterone-producing Leydig cells. However, these promoting effects decreased with an increase in the bFGF dose. Previous studies have reported that bFGF inhibits luteinizing hormone (LH)-stimulated androgen production by downregulating the mRNA expression of steroidogenic genes in immature Leydig cells. However, the expression levels of 677 microRNAs did not change significantly during the LH-mediated process of testosterone synthesis. Five microRNAs (miR-29a, -29c, -142-3p, -451 and -335) were identified, and their expression in immature Leydig cells was regulated simultaneously by bFGF and LH. These results suggested that the inhibition of LH-stimulated androgen production may be modulated by a change in bFGF-mediated microRNA expression, which further impacts the signaling pathway of testosterone biosynthesis and steroidogenic gene expression. [ABSTRACT FROM AUTHOR]
- Published
- 2014
- Full Text
- View/download PDF
23. Annexin A11 in disease.
- Author
-
Wang, Jiasheng, Guo, Chunmei, Liu, Shuqing, Qi, Houbao, Yin, Yuling, Liang, Rui, Sun, Ming-Zhong, and Greenaway, Frederick T.
- Subjects
- *
ANNEXINS , *CALCIUM channels , *GENE expression , *PHOSPHOLIPIDS , *CARRIER proteins , *CELL division - Abstract
Abstract: Ubiquitously expressed in many cell types, annexin A11 (Anxa11) is a member of the multigene family of Ca2+-regulated phospholipid-dependent and membrane-binding annexin proteins. Studies have shown that Anxa11 plays an important role in cell division, Ca2+ signaling, vesicle trafficking and apoptosis. The deregulation and mutation of Anxa11 are involved in systemic autoimmune diseases, sarcoidosis and the development, chemoresistance and recurrence of cancers. Malfunction of Anxa11 may lead to or enhance the metastasis, invasion and drug resistance of cancers through the platelet-derived growth factor receptor (PDGFR) pathway and/or the mitogen-activated protein kinase (MAPK)/p53 pathway. In a variety of diseases, Anxa11 is most commonly reported to function through interactions with apoptosis-linked gene-2 protein (ALG-2) and/or calcyclin (S100A6). Although it has been little studied, Anxa11 is a promising biomarker for the diagnosis, treatment and prognosis of certain diseases. In this review, the associations of Anxa11 with Ca2+-regulated exocytosis, cytokinesis, sex differentiation, autoimmune diseases, thrombolysis and cancers are summarized and interpreted. [Copyright &y& Elsevier]
- Published
- 2014
- Full Text
- View/download PDF
24. An iTRAQ-based mitoproteomics approach for profiling the nephrotoxicity mechanisms of ochratoxin A in HEK 293 cells
- Author
-
Shen, Xiao Li, Zhang, Yu, Xu, Wentao, Liang, Rui, Zheng, Juanjuan, Luo, YunBo, Wang, Yan, and Huang, Kunlun
- Subjects
- *
MITOCHONDRIAL proteins , *PROTEOMICS , *NEPHROTOXICOLOGY , *OCHRATOXINS , *TOXICITY testing , *REACTIVE oxygen species , *ETIOLOGY of kidney diseases , *GENE expression - Abstract
Abstract: Nephrotoxicity is the most prominent of ochratoxin A (OTA) among the diverse range of toxicological effects. Previous work indicated that reactive oxygen species (ROS) play an important role in the pathogenesis of a variety of renal diseases, and its major endogenous source is mitochondria. No research has used global protein expression profiling to investigate potential toxicity mechanisms of OTA at the mitochondria level. An iTRAQ-based mitoproteomics approach was used to explore possible toxicity mechanisms of OTA and potential protective mechanisms of N-acetyl-L-cysteine (NAC) using the mitochondria of Human Embryonic Kidney 293 (HEK 293) cells. Our results showed that OTA induced a decrease in ΔΨm, and an increase in ROS and cell death. We identified a total of 1973 nonredundant proteins, among which 1398 proteins (70.86%) were overlapped. There were 66 significantly different proteins expressed in response to OTA, which were mainly involved in the perturbation of the mitochondrial electron transport chain (mETC), inhibition of protein synthesis, and induction of stress response and cell death. In addition, NAC could almost completely reverse the adverse effects of OTA at the protein level. Finally, a hypothetical model of OTA-induced mitochondria damage is proposed to provide a framework for the toxicity mechanism of OTA. [Copyright &y& Elsevier]
- Published
- 2013
- Full Text
- View/download PDF
Catalog
Discovery Service for Jio Institute Digital Library
For full access to our library's resources, please sign in.