1. Effect of red seaweed sulfated galactans on initial steps of complement activation in vitro.
- Author
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Sokolova EV, Kravchenko AO, Sergeeva NV, Kalinovsky AI, Glazunov VP, Bogdanovich LN, and Yermak IM
- Subjects
- Antibodies blood, Carbohydrate Conformation, Carbohydrate Sequence, Carrageenan chemistry, Carrageenan pharmacology, Complement Pathway, Alternative drug effects, Complement Pathway, Classical drug effects, Galactans blood, Humans, In Vitro Techniques, Molecular Structure, Nuclear Magnetic Resonance, Biomolecular, Polysaccharides chemistry, Polysaccharides pharmacology, Rhodophyta chemistry, Spectroscopy, Fourier Transform Infrared, Sulfates chemistry, Complement Activation drug effects, Galactans chemistry, Galactans pharmacology, Seaweed chemistry
- Abstract
The research described here presents data on the effect of galactans of red algae, carrageenans (λ/μ/ν-, κ-, κ/β-, and ι/κ-types), and agar on complement system activation in normal human serum. The experiments were based on well surfaces coated with triggering agents for binding initiating complement components -C3 and C4. The sulfated galactans inhibited C3 binding to lipopolysaccharide with direct dependence on the sulfation degree of polysaccharides. Sulfation degree was also important in carrageenans' capacity to reduce C4 binding to mannan. However, C4 binding to antibodies was considerably activated by carrageenans, especially with 3,6-anhydrogalactose. The gelling carrageenans were able to block antigen binding centers of total serum IgM and with more intensity than non-gelling. No structural characteristics mattered in ameliorating C5 cleavage by plasmin in extrinsic protease complement activation, but λ/μ/ν- and κ/β-carrageenans almost completely inhibited C5 cleavage. Thus, galactans participated in cell surface biology by imitating surface glycans in inhibition of C3 binding and mannose binding lectin, but as to the tthe heclassical pathway these substances stimulated complement, probably due to their structure based on carrabiose., (Copyright © 2020 Elsevier Ltd. All rights reserved.)
- Published
- 2021
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