Back to Search
Start Over
Effect of red seaweed sulfated galactans on initial steps of complement activation in vitro.
- Source :
-
Carbohydrate polymers [Carbohydr Polym] 2021 Feb 15; Vol. 254, pp. 117251. Date of Electronic Publication: 2020 Oct 21. - Publication Year :
- 2021
-
Abstract
- The research described here presents data on the effect of galactans of red algae, carrageenans (λ/μ/ν-, κ-, κ/β-, and ι/κ-types), and agar on complement system activation in normal human serum. The experiments were based on well surfaces coated with triggering agents for binding initiating complement components -C3 and C4. The sulfated galactans inhibited C3 binding to lipopolysaccharide with direct dependence on the sulfation degree of polysaccharides. Sulfation degree was also important in carrageenans' capacity to reduce C4 binding to mannan. However, C4 binding to antibodies was considerably activated by carrageenans, especially with 3,6-anhydrogalactose. The gelling carrageenans were able to block antigen binding centers of total serum IgM and with more intensity than non-gelling. No structural characteristics mattered in ameliorating C5 cleavage by plasmin in extrinsic protease complement activation, but λ/μ/ν- and κ/β-carrageenans almost completely inhibited C5 cleavage. Thus, galactans participated in cell surface biology by imitating surface glycans in inhibition of C3 binding and mannose binding lectin, but as to the tthe heclassical pathway these substances stimulated complement, probably due to their structure based on carrabiose.<br /> (Copyright © 2020 Elsevier Ltd. All rights reserved.)
- Subjects :
- Antibodies blood
Carbohydrate Conformation
Carbohydrate Sequence
Carrageenan chemistry
Carrageenan pharmacology
Complement Pathway, Alternative drug effects
Complement Pathway, Classical drug effects
Galactans blood
Humans
In Vitro Techniques
Molecular Structure
Nuclear Magnetic Resonance, Biomolecular
Polysaccharides chemistry
Polysaccharides pharmacology
Rhodophyta chemistry
Spectroscopy, Fourier Transform Infrared
Sulfates chemistry
Complement Activation drug effects
Galactans chemistry
Galactans pharmacology
Seaweed chemistry
Subjects
Details
- Language :
- English
- ISSN :
- 1879-1344
- Volume :
- 254
- Database :
- MEDLINE
- Journal :
- Carbohydrate polymers
- Publication Type :
- Academic Journal
- Accession number :
- 33357847
- Full Text :
- https://doi.org/10.1016/j.carbpol.2020.117251