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Glycemic status depicts a state of individual blood glucose level. Biochemical parameters to identify glycemic status associated with diabetes mellitus include fasting or non-fasting blood glucose, HbA1c, and oral glucose tolerance. Our present work conducted a systematic review aiming to systematically summarize the in vivo experimental results of nutmeg treatments towards the glycemic status of rats and mice. The systematic review followed PRISMA guidelines. PubMed, ProQuest, Wiley Online Library, and Google Scholar were used as databases for searching articles. Twenty-one articles that met the criteria were eventually used in this systematic review. The results of the in vivo studies revealed that the intervention of nutmeg samples in rats and mice had a positive effect. This finding was present in all reviewed articles. Overall, treatment by nutmeg samples was able to attenuate fasting blood glucose, non-fasting blood glucose, and HbA1c in rats and mice, reaching up to 94.52, 60.76, and 48.86%. We also found that nutmeg samples prepared from mace and methanol had the highest effect in reducing blood glucose. Based on this systematic review, it can be concluded that nutmeg has a positive effect on improving glycemic status and reducing the risk of DM in rats and mice.

The application of plant extracts (PEs) could be a promising option to satisfy consumers' demand for natural additives to inhibit growth of variable pathogenic bacteria. Thus, the aim of this study was to develop a standardized microdilution method to examine the antimicrobial effects of 10 hydrophilic PEs against two strains of Clostridium perfringens facing various food-relevant influencing factors. Because of the high opacity of PEs, resazurin was used as an indicator for bacterial growth instead of pellet formation. The highest value of the MIC of the replications of each PE was defined as effective plant extract concentration (EPC), whereas the next concentration beneath the lowest MIC was defined as the ineffective plant extract concentration (IEPC). The EPCs of seven PEs, allspice, cardamom, cinnamon, clove, coriander, ginger, and mace, were between 0.625 and 10 g/kg, whereas extracts of caraway, nutmeg, and thyme showed no antimicrobial activity up to the maximum concentration tested (10 g/kg) against C. perfringens in vitro. Two intrinsic factors, sodium chloride (NaCl) and sodium nitrite (NaNO2), displayed either synergistic or additive effects or no interaction with most PEs. By combination with PEs at their IEPC (0.08 to 1.25 g/kg), MIC of NaCl and NaNO2 decreased from between 25 and 50 g/kg to between 6 and 25 g/kg and from more than 200 mg/kg to between 0.2 and 100 mg/kg, respectively. In contrast, lipid (sunflower oil) at a low concentration inhibited the antimicrobial effects of all tested PEs. For extrinsic factors, only allspice, ginger, and coriander could maintain their antimicrobial effects after being heated to 78°C for 30 min. The synergistic effect between PEs and pH values (5.0 and 5.5) was also found for all PEs. The established screening method with resazurin and defining EPC and IEPC values allows the verification of antimicrobial effects of PEs under various food-relevant influencing factors in a fast and reproducible way. HIGHLIGHTS

The aim of the study was to develop and compare the predictive models of lipid oxidation in minced raw pork meat enriched with selected plant extracts (allspice, basil, bay leaf, black seed, cardamom, caraway, cloves, garlic, nutmeg, onion, oregano, rosemary and thyme) by investigation TBARS values changes during storage at different temperatures. Meat samples with extract addition were stored under various temperatures (4, 8, 12, 16, and 20°C). TBARS values changes in samples stored at 12°C were used as external validation dataset. Lipid oxidation was evaluated by the TBARS content. Lipid oxidation increased with storage time and temperature. The dependence of lipid oxidation on temperature was adequately modelled by the Arrhenius and log-logistic equation with high R2 coefficients (0.98–0.99). Kinetic models and artificial neural networks (ANNs) were used to build the predictive models. The obtained result demonstrates that both kinetic Arrhenius (R2 = 0.83) and log-logistic (R2 = 0.84) models as well as ANN (R2 = 0.99) model can predict TBARS changes in raw ground pork meat during storage.

This study aimed to obtain yeast and bacteria from Myristica fragrans Houtt., which have the potential to produce chitinase enzymes with antagonistic ability against Rigidoporus microporous. Both microorganisms were extracted from the leaves and fruit of nutmeg. A total of 35 yeast and 29 bacterial isolates were obtained, with different morphological characters. The chitinolytic test was carried out qualitatively, and the parameters observed include the clear zones around the colony. A total of 4 bacterial isolates produced chitinase enzymes (BP 1.2.1, BP 2.1.1, EPBj II.K1, and EPBj II. K2) with a chitinolytic index of 3.92, 5.38, 2.00, and 1.66, respectively. Yeast isolates were negative for chitinase enzymes. The difference in index value indicated a variation in enzyme activity. The antagonist test was carried out using a dual culture method. A total of 1 yeast and 14 bacterial isolates inhibited the growth of R. microporous, and each has a different inhibitory zone. Based on the percentage of inhibition value, the highest percentage occurred in P.K1(41.1%), P. K2 (50%), dan EPBj II. K6 (42.2%). The antagonist test indicator includes the formation of inhibitory zones on the medium. Hemolysis test showed that yeast and bacteria are not able to break down blood cells in the medium. The molecular identification showed that P. K1 and P. K2 isolates were classified as Bacillus subtillis and EPBj II. K6 were identified as Bacillus aerius with 100% sequence homology and 99% bootstrap value respectively. These findings provided information about potential microbes that control white root fungus.

Flour and protein concentrate of African nutmeg (Pycnanthus angolensis) were subjected to standard analytical techniques to evaluate nutrient composition. Protein content was 27.40% and 60.72% for ...

Nutmeg pulp is one part of the nutmeg plant that contain antioxidant to prevent free radicals in the body. Nutmeg pulp contains secondary metabolites of the flavonoid class which have function as antioxidant. This study was conducted to determine the effect of drying the nutmeg pulp on the level of antioxidant activity using the DPPH method. After measuring the antioxidant activity of wind-dried and oven–dried nutmeg using the DPPH (1,1-diphenyl-2-picrylhydrazyl) method, the results showed that the antioxidant activity (IC50) of wind dry is 116,952 µg/mL and oven dry is 219,2 µg/mL. The result of one-way Anova data analysis obtain p-value (p)

Daun pala (Myristica fragrans Houtt.) memiliki senyawa metabolit sekunder seperti flavonoid, saponin dan tanin. Oleh karena itu, daun pala dikembangkan ke dalam bentuk sediaan gel hand sanitizer yang berpotensi sebagai antibakteri. Dalam formulasi gel, penggunaan gelling agent dan humektan merupakan faktor penting yang dapat menghasilkan sediaan gel yang stabil. Tujuan dari penelitian ini yaitu untuk mengetahui stabilitas sediaan gel hand sanitizer ekstrak etanol daun pala dengan variasi konsentrasi HPMC dan gliserin. Gel hand sanitizer ini dibuat 3 formulasi dengan variasi HPMC dan gliserin, yaitu formula 1 (HPMC 1% : gliserin 5%), formula 2 (HPMC 1.5% : gliserin 7.5%), formula 3 (HPMC 2%, gliserin 10%). Uji stabilitas yang dilakukan terhadap sediaan menggunakan metode cycling test sebanyak 6 siklus. Berdasarkan hasil analisis cycling test 6 siklus dihasilkan bahwa formula yang memiliki stabilitas paling baik adalah formula 1 dengan variasi konsentrasi HPMC 1% dan gliserin 5%.

Myristicin, a potential toxicant, is a major ester present in the pericarp of nutmeg (Myristica fragrans) and alcohol acyltransferase (AAT) is responsible for its generation. The objective of the study was firstly to estimate the effect of blanching and pickling process on the inactivation of AAT and production of myristicin in nutmeg pericarp. Secondly, the effect of pickling process on the physical, microbial, antioxidant and sensory properties of nutmeg was evaluated. The nutmeg fruit pericarp was water-blanched for 0, 3, 5, 7 and 9 min. The increased blanching time significantly (p

Nutmeg oleoresin is one of the nutmeg derivative products with high values produced from the extraction process. The conventional extraction process using maceration takes a long time so that it is less suitable for industries needs. This study aims to examine the UAE direct sonication method for increasing yield and quality of nutmeg eleoresin. Nutmeg with optimal maturity level were harvested, dried and milled into 60 mesh particle size. Nutmeg powder of 200 g was placed in beaker glass filled with etanol 800 ml (material and solvent ratio 1:4) and UAE was carried out using a sonicator with a frequency of 20 kHz and power of 700 W. Four ultrasound amplitude levels (45, 60, 75, 90%) and four levels of extraction time (30, 45, 60, 75 minutes) were taken as UAE treatments. Extraction using maceration at room temperature for 7 hours was done as control. The results showed that the greater the amplitude of the ultrasound, the higher the yield of nutmeg oleoresin. The longer the extraction time, the higher the yield of nutmeg oleoresin. Ultrasonic assisted extraction can increase nutmeg oleoresin yield by 11 to 52% and faster time (

A study was conducted to evaluate the effects of three different spices of Monodora myristica (African Nutmeg), Zingiber officinale (Ginger), and Piper guineense (African Black Pepper) on nutrient composition and organoleptic characteristics of dried rabbit meats. The three spices collected as seeds were processed into meals and applied on the fresh meat cut (loin) obtained from 10 rabbit bucks. The treatments were arranged as: T (200g meat without 1 spice) which served as control, T (200g meat +5% Nutmeg) T (200g meat + 10% Nutmeg) 2 3 ,T (200g meat + 5% African Black Pepper) T (200g meat +10%) T (200g meat +5% 4 5 6 Ginger) T (200g meat +10% Ginger). Each treatment was oven-dried before presented to 7 taste panel that adjudged the test for colour, flavour, tenderness, juiciness and overall acceptability. Samples of each spice and the processed meat were analyzed for nutrient composition. Result obtained showed that the meat is made up of protein, 50.52%, fat 6.45%, ash 5.05%, fibre 3.23%. Proximate composition of Ginger showed that it was significantly (P0.05) from the control while the least score was recorded in Piper guineense. Similarly, nutritional composition of meats improved in terms of protein, ash and fibre significantly (P

Dodol is a semi-wet food that has a plastic texture. The typical organoleptic properties of dodol are brown and have a sweet, savory and legit taste with a plastic texture. Nutmeg meat has the potential to be processed into various food products such as candied nutmeg, nutmeg syrup, jam, nutmeg juice, instant nutmeg drink, nutmeg jelly, nutmeg wine, vinegar acid, gelatin hard candy and dodol. Processing nutmeg meat into nutmeg dodol can add value to nutmeg products and increase farmers' income. The purpose of this study was to produce nutmeg dodol with the best organoleptic properties. Dodol from nutmeg meat and banana flour mulu bebe produces a colour value of 3.64-3.70 (dislike), flavour 3.25-3.59 (rather dislike), and aroma of 3.75-4.15 (dislike). The ratio of the addition of nutmeg meat 65% and banana flour mulu bebe 35% meets the quality of SNI dodol, the ratio of addition of nutmeg meat 45% and banana flour mulu bebe 55% produces nutmeg dodol favoured by consumers.

The article describes the varieties of elderberry, and the process of black elderberry fruits ripening. External characteristics of flowers, followed by ripening of fruits, have been described. The main components useful for the human body have been identified, which are contained in the fruits of the black elderberry. The effect of elderberry fruits on the human body when used for medicinal purposes has been. Elderberry fruits have a coloring substance that must be added to confectionery creams, sweets, marshmallows, as well as to liqueurs and wine products to give the products color and nutmeg taste. Elderberry fruits can be eaten fresh, ripe, but the most delicious and more useful fruits are provided in processed form, especially in juice and dried form. The useful properties of elderberry have been described, as well as the dangerous properties that appear in case of eating unripe elderberry fruits growing in the Republic of Belarus. The detailed composition of the components of elderberry fruit has been considered, all its useful properties noted. Particular attention is paid to the chemical composition of elderberry fruit, detailed amino acid composition of elderberry fruit has been given, and the composition of anthocyanin content in juice of direct extraction from elderberry fruit has been shown. The use of elderberry pomace in the food industry for the preparation of jam, jams, confiture, preserves and other products, as well as adding to wine, alcoholic beverages, to impart nutmeg taste, in the confectionery industry to add to dough and giving the product an almond smell has been described. It is especially noted that the pomace from elderberries contains biological active substances, bioflavonoids, anthocyanins, which confirms their immediate importance and the need to use for the creation of many products for preventive and functional purposes in the food industry.

Beef rendang is a popular traditional food from West Sumatra which is commonly cooked by using fresh seasoning such as herbs and spices yet not all types of those ingredients are effortlessly available to be found. This issue hinders the local community to cook beef rendang with the original taste of West Sumatra. As a matter of fact, producing dried seasoning to cook rendang could be a possible solution to solve this issue. This study aimed to compare the sensory analysis of beef rendang by using fresh and dried seasoning. This sort of research was a pure experiment with a completely randomized design and 3 repetitions. The ingredients being used are beef, coconut milk, chilies, ginger, galingale, lemongrass, shallots, garlic, lime leaves, bay leaves, turmeric leaves and salt, cooking oil, nutmeg and coriander purchased at traditional markets. The seasoning was sautéed and the coconut milk was poured into the pan, then the beef was added onto the coconut milk with the seasoning until it is half cooked in which the name is Kalio (Like curry, but not become rendang yet), then it was heated up and dried up for hours until the coconut milk dried and turned black which is called rendang. In addition, the data collection technique was carried out by giving a questionnaire to the panelists. The data analysis used was the t-test independent sample t-test using SPSS 15.0 software. The results obtained from the research showed that the average values of the sensory evaluation of the two rendang were almost similar. The average values of beef rendang sensory evaluation using fresh and dried seasoning, respectively, were colour 3.26 and 3.53; aroma 4.0 and 4.13; the texture has the same value, namely 3,4; taste namely 4,6 and 4,46 and the hedonic test 4,33 and 4,20. These results were likely identical as what the statistical test attained (t-test) that there are no significant differences between beef rendang using dried seasoning and fresh seasoning for each sensory testing.

Eight plant essential oils (cardamom, cinnamon, clove, eucalyptus, lemongrass, lime, nutmeg and rosemary) were evaluated for their antimicrobial activity against three Pseudomonas species associate...

Pickled nutmeg which is made from the osmotic dehydration process is one of the well-known and a trademark delicacy in Penang, Malaysia. Despite of the high demand from the consumers, the toxicant volatile compound of myristicin that is produced along the making process has become the limitation of the pickled nutmeg consumption. Hence, pre-treatments of different blanching period prior to osmotic dehydration process were suggested to mitigate the issue. Analysis of the texture, myristicin content, and alcohol acyltransferase (AAT) activity were undergone to the fresh (control), pretreated nutmeg pericarp, and post-pretreated pickled nutmeg. The nutmeg samples were prepared and subjected to various blanching period (0, 3, 5, 7, and 9 min) at 100 ℃ in distilled water, followed by immersing the samples into sodium metabisulfite/citric acid (SMB/CA) solution for 30 min. Then, the pretreated nutmeg pericarp underwent the osmotic dehydration process by using food grade sodium chloride. The increase in blanching period resulted in the reduction of texture hardness, myristicin content, and AAT activity of the pretreated nutmeg pericarp compared to the control. In overall, 7 min blanching period was preferable, as the myristicin level in nutmeg sample could not be detected and the AAT activity was at its lowest level.

Clean label products with natural antioxidants have always attracted great attention due to healthy benefits. In this study, the stability of palm olein oil with various natural antioxidants (cedarwood, citronella, clove, nutmeg and rosemary oil) during the heat treatment was investigated; the acidic value, conjugated diene and triene content, peroxide value, thiobarbituric acid reactive substance value and instrumental color of the palm olein oil were analyzed. Sample with nutmeg oil exhibited the highest oxidation stability. In addition, the higher the nutmeg oil concentration, the better the prevention of oil oxidation during the heat treatment. During the repeated frying with corn extrudate, nutmeg oil concentration of 4 g/kg palm olein oil was able to prevent lipid degradation. The acidic and peroxide value of the palm olein oil in the fryer was statistically similar to that of the extracted oil from the fried extrudate. Nutmeg oil can be considered as a potential natural antioxidant for industrial frying of snack food products.

Microencapsulation is employed to protect bioactive ingredients in foods and is also used for their controlled release at targeted sites. The objectives of this study were to examine the bio active properties and stability of microcapsules containing nutmeg oleoresin encapsulated with different ratios (2:1, 1:1 and 1:2) of gum arabic and sorghum starch (native and OSA modified) prepared by freeze-drying. The properties of nutmeg microcapsules, including oxidation stability, antioxidant activity, total phenolic content and total flavonoid content were evaluated after sixty days storage trial. The finding revealed that freeze drying method had a spectacular effect on the retention of bioactive compounds during storage that consequently improved the storage stability of the all nutmeg encapsulated powders. The sample composed of 1:2 ratio of gum arabic with OSA starch resulted to have lowest peroxide value after 60 days of storage. Furthermore, the microcapsules were also observed through Fourier Transform Infrared spectroscopy and Scanning electron microscopy for advance analysis.

A number of phytochemicals or secondary metabolites were produced by herbs and spices. These were used as food preservatives and also as traditional medicines from long back. These were having various pharmacological benefits which included antioxidant, analgesic, anticancer, anti-inflammatory and anti-pyretic properties. In the present study two different plant species (Zingiber officinale, Myristica fragrans) were used for studying their antimicrobial and antibiofilm activity. The phytochemical analysis of the plant extracts was done by using standard methodology. FTIR analysis of the ginger and nutmeg extracts were carried out to study the functional groups present in the respective plant extracts. The extracts were also subjected to NMR spectroscopy. Two different bacteria were used for the studies which are good biofilm producers, Pseudomonas and E.coli. The antimicrobial activity of the methanolic and choloroform extracts of ginger and nutmeg were studied using air liquid interphase coverslip assay and biofilm assay. The results suggest that the ginger and nutmeg extracts have biofilm inhibition activity. The reduction in biofilm was observed in all the 3 concentrations of the nutmeg chloroform extract with maximum biofilm reduction in 100% and was compared with the control. Both Pseudomonas aeruginosa and Escherichia coli, biofilm inhibition was observed in all 3 concentrations of ginger and nutmeg.

The deep‐frying process for plenty of fried products using vegetable oils needs safe and effective antioxidants. In the present exploration, the nutmeg essential oil (NEO) was employed as a potential antioxidant for sunflower oil during the deep‐frying of Chinese Maye at 180°C for 30 hr. In the comparative study, the additions for NEO at 0.12 g/kg, TBHQ at 0.12 g/kg, BHA at 0.08 g/kg, and BHT at 0.08 g/kg to sunflower oil were able to obviously improve its oxidative stability during the deep‐frying process, and their antioxidant effects were in the relative order: TBHQ at 0.12 g/kg > NEO at 0.12 g/kg > BHA at 0.08 g/kg > BHT at 0.08 g/kg (p, The deep‐frying process for plenty of fried products using vegetable oils needs safe antioxidants. In the present study, the nutmeg essential oil (NEO) and limonene were demonstrated to be effective in inhibiting the oxidation of sunflower oil during the deep‐frying of Chinese Maye at 180°C for 30 hr and improving the sensory properties of the fried products, Chinese Maye. Therefore, both of them could be used as antioxidants in the deep‐frying process of sunflower oil.

Nutmeg (Myristica fragrans Houtt) is a medicinal plant that produces essential oils and is often used as a flavoring of food and medicine. The process of extracting essential oils from the nutmeg fruit pulp using the steam-water distillation method. The yield of essential oil obtained at 0.07765%, with a pale yellow color, the distinctive smell of nutmeg oil with a refractive index of 1.485, and a specific gravity of 0.909 g / mL and soluble in ethanol. The results of GC-MS identification showed that the essential oil of nutmeg fruit flesh contains five components, the main compound is myristicin (18.44%), α-pinene (14.2%), limonene (9.37%), γ-terpinene (7.98%) and terpinolene (7.66%). Based on the test results of anti-inflammatory activity, the value of the percentage of red blood cell stability optimum mounting to 80.21% at a concentration of 10 ug / mL, where the standard aspirin has an activity of 72.26%. These results indicate that the meat nutmeg essential oil is potential as anti-inflammatory agents.

Historically, the analysis of citrinin has mainly been performed on cereals such as red yeast rice, however, in recent years, more complex and abnormal commodities such as spices and infant foods are becoming more widely assessed. The aim of this study was to develop and validate clean-up methods for spices and cereal-based infant foods using a citrinin immunoaffinity column before HPLC analysis with fluorescence detection. Each method developed was validated with a representative matrix, spiked at various citrinin concentrations, based around European Union (EU) regulations set for ochratoxin A (OTA), with recoveries &gt, 80% and % RSD &lt, 9% in all cases. The limit of detection (LOD) and the limit of quantification (LOQ) were established at 1 and 3 µg/kg for spices and 0.1 and 0.25 µg/kg for infant cereals, respectively. These methods were then tested across a variety of spices and infant food products to establish efficacy with high recoveries &gt, 75% and % RSD &lt, 5% across all matrices assessed. Therefore, these methods proved suitable for providing effective clean-up of spices and infant cereals, enabling reliable quantification of citrinin detected. Samples such as nutmeg and infant multigrain porridge had higher levels of citrinin contamination than anticipated, indicating that citrinin could be a concern for public health. This highlighted the need for close monitoring of citrinin contamination in these commodities, which may become regulated in the future.

During the shelf-life, meat undergoes a number of processes that negatively affect the quality of the product, including fatty acid composition. The application of various plant extracts in meat could affect the changes of fatty acids during storage. Thus, the aim of this study was to investigate the effect of various spice and herb extracts on fatty acid composition in raw pork, beef, and chicken meat when stored at 4 °C for 13 days. Based on multivariate statistical analysis, two datasets were extracted from each type of meat. One dataset included samples with allspice, bay leaf, black seed, cardamom, caraway, clove, and nutmeg with the high share of total MUFA (monounsaturated fatty acids) in chicken and pork meat and high MUFA and PUFA (polyunsaturated fatty acids) contribution in beef meat after storage. The second dataset included basil, garlic, onion, oregano, rosemary, and thyme with high PUFA share in chicken and pork meat and high SFA (saturated fatty acids) contribution in beef meat. From the regression analysis, a significant effect of time on fatty acid composition in meat was reported. Generally, the rates of fatty acid changes were dependent on the plant extract incorporated into the meat. The most visible effect of plant extracts was obtained in chicken meat. In chicken meat with plant extracts, the rates of SFA and PUFA changes with time were slower compared to the control sample. In summary, the fatty acid composition of intramuscular fat varied during storage, and the addition of plant extracts significantly affected the rate of these changes, which was dependent on the meat matrix.

In this study, predictive models of protein oxidation, expressed as the content of thiol groups (SH), in raw ground pork were established and their accuracy was compared. The SH changes were monitored during, maximum, 11 days of storage at five temperature levels: 4, 8, 12, 16, and 20 °C. The effect of 13 plant extracts, including spices such as allspice, black seed, cardamom, caraway, cloves, garlic, nutmeg, and onion, and herbs such as basil, bay leaf, oregano, rosemary, and thyme, on protein oxidation in pork was studied. The zero-order function was used to described SH changes with time. The effect of temperature was assessed by using Arrhenius and log–logistic equations. Artificial neural network (ANN) models were also developed. The results obtained showed very good acceptability of the models for the monitoring and prediction of protein oxidation in raw pork samples. High average R2 coefficients equal to 0.948, 0.957, and 0.944 were obtained for Arhhenius, log-logistic and ANN models, respectively. Multiple linear regression (MLR) was used to assess the influence of plant extracts on protein oxidation and showed oregano as the most potent antioxidant among the tested ones in raw ground pork.

We investigated the larvicidal potential of 10 plant essential oils (EOs) against the Asian tiger mosquito Aedes albopictus. Among the EOs, larvicidal activity against Ae. albopictus was strongest in those derived from massoia (Massoia aromatica) and nutmeg (Myristica fragrans). Larvicidal activities of massoia and nutmeg EOs against Ae. albopictus were 95.0% and 85.0% at 50 μg/mL, respectively. A total of 4 and 14 compounds were identified from massoia and nutmeg, respectively, and two massoia lactones, C10 and C12, were isolated from massoia EO. Among the identified compounds, benzyl salicylate, terpinolene, C12 massoia lactone, sabinene, benzyl benzoate, methyl eugenol, and C10 massoia lactone exhibited the strong larvicidal activity. Cellulose nanocrystal (CNC)-stabilized Pickering emulsions of massoia and nutmeg EOs were developed to overcome the insolubility of EOs in water. CNC/massoia and CNC/nutmeg emulsions were stable for at least 10 days, and larvicidal activities of CNC/massoia PE and CNC/nutmeg were higher than those of crude massoia and nutmeg EOs. This study presents a CNC-stabilized PE, a suitable formulation for EOs, as a potential larvicide against Ae. albopictus.

Simple Summary Demand for poultry meat (chickens and turkeys) is constantly increasing. The upward trend in the production and consumption of poultry meat has two reasons. The first is the financial aspect because chicken meat is relatively cheap. The second reason is the nutritional and health aspect. Although the meat has high nutritional, dietary, culinary, technological, and sensory values, it is very susceptible to undesirable changes during storage, mainly due to the growth of microflora but also due to lipid and protein oxidation. The use of plant extracts in food technology is multifunctional, as they exhibit antioxidant and antibacterial effects and have a beneficial effect on the texture of meat and meat products. Moreover, the antioxidant effect of compounds isolated from plants may influence consumer health. Antioxidants of plant origin can be used as an additive to animal feed, as well as a component of stuffing or marinating mixes for meat. In addition, they are used in the coating of raw materials or in active packaging for food products. So far, many studies have shown the positive effect of plant and plant extract addition to meat on the oxidative status of its protein. However, the predictive approach to protein oxidation in raw meat is still little described. This study has demonstrated the potential usefulness of the kinetic model as well as models based on artificial neural networks (ANNs) to the realistic prediction of protein oxidation expressed as thiol group (SH) changes in raw and cooked chicken meat during storage. Such predictive models allow us to predict oxidative changes in minced meat under different time and temperature conditions as minced meat is particularly susceptible to oxidation through exposure to oxygen during the mincing process itself and through the increased contact surface with oxygen. This knowledge is very useful in designing food products and predicting their shelf-life. Additionally, the effectiveness of various spices in the raw and cooked meat system were compared. Meat is a very complex system and, according to the research, there is no direct correlation between the anti-oxidant activity of the spice itself and its antioxidant effectiveness in the product. Abstract The aim of the study was to develop predictive models of thiol group (SH) level changes in minced raw and heat-treated chicken meat enriched with selected plant extracts (allspice, basil, bay leaf, black seed, cardamom, caraway, cloves, garlic, nutmeg, onion, oregano, rosemary, and thyme) during storage at different temperatures. Meat samples with extract addition were stored under various temperatures (4, 8, 12, 16, and 20 °C). SH changes were measured spectrophotometrically using Ellman’s reagent. Samples stored at 12 °C were used as the external validation dataset. SH content decreased with storage time and temperature. The dependence of SH changes on temperature was adequately modeled by the Arrhenius equation with average high R2 coefficients for raw meat (R2 = 0.951) and heat-treated meat (R2 = 0.968). Kinetic models and artificial neural networks (ANNs) were used to build the predictive models of thiol group decay during meat storage. The obtained results demonstrate that both kinetic Arrhenius (R2 = 0.853 and 0.872 for raw and cooked meat, respectively) and ANN (R2 = 0.803) models can predict thiol group changes in raw and cooked ground chicken meat during storage.

The aim of the study was to develop and compare the predictive models of lipid oxidation in minced raw beef meat enriched with selected plant extracts (allspice, basil, bay leaf, black seed, cardamom, caraway, cloves, garlic, nutmeg, onion, oregano, rosemary and thyme) expressed as value changes of TBARS (thiobarbituric acid reactive substances) in various time/temperature conditions. Meat samples were stored at the temperatures of 4, 8, 12, 16 and 20 °C. The value changes of TBARS in samples stored at 12 °C were used as the external validation dataset. Lipid oxidation increased significantly with storage time and temperature. The rate of this increase varied depending on the addition of the plant extract and was the most pronounced in the control sample. The dependence of lipid oxidation on temperature was adequately modeled by the Arrhenius and log-logistic equation with high average R2 coefficients (≥0.98) calculated for all extracts. Kinetic models and artificial neural networks (ANNs) were used to build the predictive models. The obtained result demonstrates that both kinetic Arrhenius (R2 = 0.972) and log-logistic (R2 = 0.938) models as well as ANN (R2 = 0.935) models can predict changes in TBARS in raw ground beef meat during storage.

African nutmeg, a possible local substitute for a commercial food flavourant, remains largely underutilized in Nigeria. Its application potential in cookie production was investigated in this study. African nutmeg ( Monodora myristica ) seed flour (ANM) was produced using standard method. The flour was substituted for vanilla flavour (VFL) in ratio of 0, 1, 2, 3, and 3.5 g and functional properties of the flour blends (water absorption capacity (WAC), oil absorption capacity (OAC), and bulk density) were determined, using standard methods. Cookies were developed and characterized chemically, physically (colour) and organoleptically using the AO A C method, a colourimeter and sen s o r y panellists r e spect i v e l y . Data were analysed using ANOVA at p< 0.05 . Replacement of vanilla with African nutmeg had no significant effect on bulk density (0.62g/cm 3 – 0.68 g/cm 3 ), but significantly affected WAC (133-142%) and OAC (147-156%) of flour blends. Crude protein (9.44-15.49%), crude fat (3.17-6.50%), total ash (2-2.73%) and crude fibre (0.12-0.23%) contents of the cookie increased, whilst metabolizable energy (385.33-367kcal) decreased. There were reductions in pH (6.83-6.53) and TSS (5.80-1.20). Brown index of the snack increased with addition of nutmeg. Antioxidant indicators (flavonoids, DPPH and phenol) varied among cookie samples. Antinutrients, saponin, tannin and oxalate, were within tolerable limits. All cookie samples were judged a cc ep tab l e b y the p a n ellists , with SLZ being the most acceptable. An acceptable and nutritious snack was produced at 100% replacement. M. myristica seed could serve as substitute for vanilla in the production of cookies and other related snacks.

In recent years, demands for minimal processing and free-synthetic preservatives are increasing because of growing concern among consumers regarding the safety issues of additives. Nutmeg (Myristica fragrans Houtt.) has been used as a spice and traditional medicine in Asian countries. This study aimed to determine the antibacterial activity of nutmeg extract against foodborne pathogens on raw beef during storage. Nutmeg seeds were extracted using a maceration method with methanol as a solvent. The extract was assessed for antibacterial activity against a range of microorganisms using the disc diffusion assay, minimum inhibitory concentration and minimum bactericidal concentration. The effect of heat and different pH of the extract on its antibacterial activity was also conducted to evaluate the stability of the extract. The effects of the extract at different concentrations on the microbial population of the raw beef during chilled (4.0±0.2oC) and frozen (-18.0±0.2oC) storage for 21 days were then evaluated. The nutmeg extract exhibited antimicrobial activity against the range of microorganisms tested, which was stable at high temperature (80.0±2.0oC) and in the pH range tested (3.0, 6.8, and 11). Furthermore, the extract significantly inhibited microbial growth on raw beef stored for 21 days in chilled or frozen conditions, indicating that the nutmeg extract has the potential to be developed as a natural antimicrobial preservative in beef.

This study was carried out to evaluate the physicochemical characteristics of Ginger (Zingiber officinale), African nutmeg (Monodora myristica) and Ashanti Black pepper (Piper guineense) essential oils. Dried plant materials were obtained from the Oba market in Edo State, Nigeria. All samples were ground into a fine powder and stored in an airtight plastic container at room temperature (28±2°C) until when used. The essential oils were extracted using steam distillation and their physicochemical properties analyzed using standard procedures. The results showed that color varied from yellow for Z. officinale to colorless for M. myristica and P. guineense. The highest yield (3.55%), acid value (6.73 mg KOH/g), saponification value (185.13 mg KOH/g), free fatty acid value (3.37%) and iodine value (69.49 gI2/100 g) were recorded for M. myristica, the highest specific gravity value (0.88) was for P. guineense and M. myristica, the highest peroxide value (2.67) was for P. guineense while the highest refractive index value (1.49) was for both P. guineense and Z. officinale. There were significant differences (p

This paper proposes the use of random forest for adulteration detection purposes, combining the random forest algorithm with the artificial generation of outliers from the authentic samples. This proposal was applied in two food adulteration studies: evening primrose oils using ATR-FTIR spectroscopy and ground nutmeg using NIR diffuse reflectance spectroscopy. The primrose oil was adulterated with soybean, corn and sunflower oils, and the model was validated using these adulterated oils and other different oils, such as rosehip and andiroba, in pure and adulterated forms. The ground nutmeg was adulterated with cumin, commercial monosodium glutamate, soil, roasted coffee husks and wood sawdust. For the primrose oil, the proposed method presented superior performance than PLS-DA and similar performance to SIMCA and for the ground nutmeg, the random forest was superior to PLS-DA and SIMCA. Also, in both applications using the random forest, no sample was excluded from the external validation set.

This study examined the effect of incorporation of spray dried nutmeg oleoresin on physical and functional properties of deep fried donuts. Donuts were made by sponge and dough method with the addition of 1% of microcapsules composed of two different wall material ratios (1:1 and 1:3) of gum arabic and sorghum starch (native and octenyl succinic anhydride). The results showed that addition of microcapsules did not significantly reduce the oil uptake in donuts. All the incorporated samples possessed better retention of bioactive compounds and having better antioxidant properties with high phenolic content. Nutmeg microcapsules composed of gum arabic and native starch in a proportion of 1:1 observed to have excellent textural, sensorial and physical properties as compared to control donut sample. The supplementation of GA:NAT (1:1) also showed high antioxidant value with higher total phenolic and flavonoid content. The study revealed that only 1% addition of nutmeg microcapsules in dough formulation can be improved the physical, textural and functional attributes of deep fried donuts.

The objectives of this research were to characterize nanoemulsion from chitosan/nutmeg seed oil and to evaluate its coating on fresh strawberry which stored at 10oC for 5 days. The ultraturrax and high-pressure homogenizer were used to prepare 1.34±0.25 - 5.79±1.61 nm of nanoemulsion size which confirmed by particle size analyzer. The morphology observed by SEM that exhibited the oil globules were covered by chitosan. They were aggregated and rough droplets. Interactions among the materials were observed using FTIR which led to the presence of a new peak at 1736 cm-1 . The coated strawberry by high-pressure homogenizer-emulsion showed the best result suppressing microbial (2.41±0.01) and mould-yeast (2.78±0.10) growth at the end of storage compared to control which were 3.37±0.02 and 3.69±0.14 for microbial and mould-yeast count respectively.

Identification of Rhodamin B and methanyl yellow on fruit candies circulating in the city of Banda Aceh in a qualitative manner using the spot test method has been conducted which is chemical analysis using reagent kits. The principle of this test is by reacting a substance or sample with reagent (reagent kit) to determine the content of synthetic dyes from the substance or the sample is characterized by a distinctive color change. Samples of candied fruit obtained from traders who sell on Jl. Diponegoro, Jl. Teuku Umar, and Jl. Tgk Hasan Saleh. Based on the results of test, it can be concluded that all samples of candied fruit are yellow (liko kuning I, liko kuning II and mango) originating from Jl. Diponegoro, Jl. Teuku Umar, Jl. Tgk Hasan Saleh was not detected to contain methanyl yellow synthetic coloring, while for candied fruits that are red (liko merah I, liko merah II, nutmeg, sugar palm fruit and sago eyelets) there is one sample which contains rhodamine B, which is a sago eyelets.

Sweetmeat is the processing foods preserved with high sugar content. In the manufacture of sweetmeat not only sugar but also added lime, salt, and sulfur-containing compounds. There are two forms of processed products of sweetmeat, namely wet sweetmeat and dry sweeteat. The basic thing that differentiate both is how to make it, lasting power, and design products. This study aims to determine the level of calcium in the sample of fresh guava, ginger, and nutmeg, and to investigate the mineral calcium levels in samples of fresh and processed products as wet sweetmeat and dry sweetmeat. Each sample must first be destructed by dry destruction, then the mineral assay performed with quantitative assay atomic absorption spectrophotometry (AAS) using a gas burner nitrous oxide-acetylene at a wavelength of 422.7 nm. The advantage of this method is to have a high sensitivity (limit of detection of less than 1 ppm), and the implementation is relatively simple. Calcium levels in samples of fresh guava, ginger and nutmeg in a row each each sample as follows (7.7105 ± 0.1224) mg / 100 g, (14.5232 ± 0.117) mg / 100 g and (38, 8834 ± 0.6996) mg / 100 g. In the wet sweetmeat guava, ginger and nutmeg for (13.7787 ± 0.4123) mg / 100 g, (22.8345 ± 0.8247) mg / 100 g and (0.2284 ± 0.3759) mg / 100 g. While on dry sweetmeat guava, ginger and nutmeg for (160.9401 ± 0.4223) mg / 100 g, (176.0465 ± 1.0458) mg / 100 g and (120.4426 ± 0.7932) mg / 100 g. Levels of calcium contained in a sample of fresh guava, ginger and nutmeg and sweetmeat processed as wet and dry sweetmeat has different levels

Heterocyclic aromatic amines (HCAs) and polycyclic aromatic hydrocarbons (PAHs) as carcinogenic chemical compounds can be formed during high-temperature heating of beef patties. The current study was aimed to evaluate the effects of nutmeg and ginger essential oils incorporated in two concentrations (0.02 and 0.04%) and their nanoemulsions on the HCAs and PAHs formation in grilled beef patties during production time, 45- and 90-days storage in a freezing condition at − 18 °C. According to results, the HCAs and PAHs levels in essential oil and nanoemulsion added grilled beef patties significantly decreased while compared to the control. The addition of 0.04% nutmeg oil nanoemulsion in grilled beef patties led to the highest reduction in 2-amino-3,4-dimethyl-imidazo[4,5-f] quinoline (100% reduction) and benzo [b] fluorantene (46% reduction). The storage time of beef patties significantly affected the concentration of HCAs and PAHs. In this context, with increase in storage time, the levels of HCAs and PAHs were decreased and increased, respectively. The formation of 2-amino-3,4-dimethyl-imidazo[4,5-f] quinoline in all grilled beef patties containing nutmeg oil and its nanoemulsion was reduced by 100% after 45 and 90 days storage. However, the lowest increase in the formation of PAHs was related to dibenzo [a,h] anthracene by 25% in grilled beef patties containing 0.04% nutmeg oil nanoemulsion at the end of storage time. Generally speaking, nutmeg oil and especially prepared nanoemulsion have a great impact in preventing of the carcinogens compound formation in grilled patties.

We investigated the population growth of the khapra beetle, Trogoderma granarium Everts (Coleoptera: Dermestidae) on seven groups of commercially available non-grain commodities. Six powdered spices were used in the first group of experiments: black pepper, clove, nutmeg, allspice, cinnamon and turmeric. The second group of the tested commodities included seven animal products: powdered cow milk, powdered pork zelatin, sheeps’ wool, goat’s skin, ducks’ feathers, dry dog food and dry cat food. The third group of commodities was consisted of six herbs: oregano, spearmint, basil, coriander, laurel and marjoram. The fourth group of commodities contained ten pulses: chickpeas, lentil, split peas, black-eyed peas, beans, soybean flour and whole kernels, lima beans, mung bean, and broad beans. The fifth group comprised six dried fruits: Corinthian currants, sultanas, banana chips, melons, apricots and figs. The sixth group was consisted of five non-grain commodities: cottonseed cake, tobacco, black tea, Turk kahvesi and potato flour. The seventh group included seven nuts: sunflower seeds, pumpkin seeds, pistachios, roasted chickpeas (yellow), almonds, hazelnuts and walnuts. Finally, six cracked containment categories (0% cracked kernels, 5% cracked kernels and 95% intact kernels, 10% cracked kernels and 90% intact kernels, 25% cracked kernels and 75% intact kernels, 50% cracked kernels and 50% intact kernels and 100% cracked kernels) from five pulses were tested: chickpeas, black-eyed peas, mung bean, soybean and split peas. The highest progeny production (3.01 individuals per vial) was recorded on powdered cow milk. On pistachios, split peas, sunflower seeds, soybean flour, pumpkin seeds, walnuts, almonds and coriander, T. granarium built high population densities rapidly, while on roasted chickpeas, cottonseed cake, hazelnuts, chickpeas, dog food and lentils, its population growth was much less. Broad beans, melons, figs, lima beans, beans, Corinthian currants, pork zelatin and potato flour were less suitable diets for the development of this species. On black pepper, clove, nutmeg, allspice, cinnamon, turmeric, sheeps’ wool, goat’s skin, ducks’ feathers, cat food, sultanas, banana chips, apricots, tobacco, black tea, Turk kahvesi, oregano, spearmint, basil, laurel and marjoram, no progeny production was recorded. The proportion of 100% cracked black-eyed peas or mung beans was more suitable for the population growth of T. granarium. Also, the percentages of 50% cracked chickpeas or 10% cracked soybeans enhanced the development of the species, in comparison with the whole kernels of each pulse. Our study indicated that several non-grain commodities are beneficial for the population growth of T. granarium, a fact that should be seriously taken into account in international trade, as this pest may utilize them as “vehicles” of expansion.

Pala merupakan tanaman rempah asli Indonesia yang selama ini hanya digunakan untuk keperluan pangan seperti bumbu dan rempah, serta industri makanan. Miristisin adalah senyawa khas penentu kualitas minyak pala dan memiliki beberapa gugus fungsional, yaitu alil, fenil, dan eter. Senyawa turunan kalkon merupakan metabolit sekunder golongan flavonoid yang dikenal mempunyai aktivitas biologi seperti antikanker, sitotoksik, antimikroba dan antimalaria. Sifat antibakteri berkaitan erat dengan struktur keton α, β tak jenuh substituen yang terikat pada kedua cincin aromatiknya, seperti gugus metilendioksi, metoksi, Cl, Br, OH dan lain sebagainya. Penelitian ini bertujuan untuk mengetahui aktivitas antibakteri senyawa turunan kalkon hasil sintesis dari miristisin buah pala.Uji aktivitas antibakteri dilakukan terhadap bakteri Escheresia Coli (E. Coli) dan Staphylococcus Aureus (S. Aureus). Pengujian aktivitas antibakteri dilakukan dengan metode difusi agar dengan menggunakan cakram/disc dari larutan senyawa turunan kalkon dalam pelarut aseton dengan konsentrasi 1,25%; 2,5%, 5% dan 10% dan menggunakan kontrol positif Ciprofloxacin 5μg dan control negative pelarut aseton. Hasil pengujian kemudian dihitung zona hambat dari masing-masing konsentrasi untuk menentukan aktivitas antibakteri.Hasil uji antibakteri menunjukkan rata-rata diameter zona hambat larutan sampel pada konsentrasi 1,25%; 2,5%, 5% dan 10% pada bakteri E. Coli berturut turut 13mm, 19mm, 22 mm dan 26 mm, sedangkan pada bakteri S. Aureus berturut-turut 7 mm, 7 mm, 9 mm dan 19 mm. Hasil uji tersebut menunjukkan serbuk turunan kalkon hasil sintesis dari miristisin buah pala menunjukkan semakin besar konsentrasi sampel, semakin besar aktivitas antibakterinya. Aktivitas antibaketri kuat ditunjukkan pada bakteri E.Coli dengan konsentrasi 10%, sedangkan pada konsentrasi yang sama menunjukkan aktivitas sedang pada bakteri S.Aureus.

Pala merupakan tanaman yang digunakan dalam industri makanan, obat-obatan, parfumdan kosmetik. Minyak atsiri biji pala diketahui memiliki kandungan senyawa 1,4-terpineol dansafrol yang diduga memiliki aktivitas sebagai antibakteri.Pemisahan senyawa 1,4-terpineol dan safrol dari minyak atsiri biji pala dilakukan denganmetode distilasi fraksinasi pengurangan tekanan dengan tekanan 95 mmHg pada suhu 200-225C֯. Analisis kandungan senyawa pada minyak atsiri biji pala dan hasil pemisahan dilakukandengan GC-MS dan uji aktivitas antibakteri dengan metode difusi dan dilusi.Hasil analisis menunjukkan bahwa minyak atsiri biji pala mengandung 5 senyawadominan yaitu α-pinene, sabinene, 2-β-pinen, terpineol dan miristisin, hasil pemisahanmengandung 49,05% 1-4 terpineol dan 20,34% safrol. Hasil uji difusi menunjukkan bahwa minyakatsiri biji pala dengan konsentrasi 1,25% memiliki zona hambat sebesar 12,11 mm yang tergolongkuat, sedangkan hasil pemisahan dengan konsentrasi sama memiliki zona hambat 9,66 mm yangtergolong sedang. Hasil dilusi minyak atsiri pala dan hasil pemisahan memiliki zona bunuh yangsama pada konsentrasi 1,25%

This study deals with the development of Nutmeg (Myristica fragrans Houtt.) authentication methodology using hyperspectral imaging. Fifteen authentic samples, seven adulterant materials (i.e. 1 pericarp, 1 shell, and 5 spent samples) and 31 retail samples were used for this purpose. Furthermore, another set of adulterated nutmeg samples were artificially prepared by mixing authentic material with spent powder (5–60%). A new handheld hyperspectral imaging system was applied to obtain hyperspectral information of nutmeg powder samples in the wavelength region of 400–1000 nm. Principal Component Analysis (PCA), Partial Least Squares-Discriminant Analysis (PLS-DA) and Artificial Neural Network (ANN) models were applied for exploring the data, constructing the models, and authenticating the retail samples. The PCA showed successful spatial separation of authentic samples from adulterant materials. An ANN model predicted and showed the ability to detect adulteration at levels as low as 5% of added product-own material which was more accurate than PLS-DA model. Microscopic analysis was applied for comparison with hyperspectral imaging and to verify possible sample modification. It was concluded that the method applied here has good potential for the development of a visual quality control procedure for nutmeg powder authentication.

Quality nutmeg is characterized by a delicate aroma. Next to quality nutmeg, other – lower - grades exist on the market, such as extracted material (spent) or ground shell or dried fruit pulp. Strong fluctuations in the price of nutmeg lead to rapid changes in market dynamics and marketing opportunities, and unfortunately results in illegal commingle of ground quality nutmeg with low-grade material. In this study, we examined fingerprints of volatile and non-volatile compounds of high quality and low-grade nutmeg material by Proton Transfer Reaction Mass Spectrometry and Flow Infusion ElectroSpray Ionization Mass Spectrometry, respectively. They were compared with data from classical measurements such as total ash, acid insoluble ash, moisture and volatile oil contents. Differences in composition were examined by univariate and multivariate statistical methods. Furthermore, one-class classification models for quality nutmeg were estimated using different algorithms and their performances were examined with quality nutmeg and low-grade material, as well as mixtures thereof. Distinct differences between quality nutmeg and low-grade nutmeg samples were observed for both their volatile and non-volatile fingerprints. Intensities of volatiles and non-volatiles are highly correlated, but this phenomenon diminishes gradually and even reverses with rising molecular mass of the non-volatiles. Results showed that both techniques allowed a nearly 100% correct prediction of quality nutmeg and low-grade nutmeg samples. Therefore, both approaches are promising and with further database extension, they may become a valuable addition to the analytical authentication toolbox in addition to the classical methods and help to detect future ‘nutmeggers’.

Levels of lead (Pb), cadmium (Cd), arsenic (As), and mercury (Hg) were evaluated in 359 samples of commonly consumed spices (cinnamon, parsley, basil, oregano, coriander seed, nutmeg, cumin, bay leaf, fenugreek, rosemary, thyme, fennel, sage, clove, marjoram, tarragon, caraway, dill seed, pepper, and turmeric) from the market in the Republic of Korea. The content of Pb, Cd, and As was assessed by acid wet digestion using a microwave oven and inductively coupled plasma mass spectrometry (ICP-MS). The content of Hg was analysed using a direct mercury analyzer (DMA). Pb, Cd, As, and Hg mean content in spices ranged from 0.039-0.972 mg kg-1, 0.013-0.315 mg kg-1, 0.121-0.861 mg kg-1, and 0.001-0.025 mg kg-1, respectively.

Nutmeg is a plant grown in tropical region for its seeds distinct flavor, nutritive value and health benefits. Despite extensive studies on nutmeg seed metabolites composition, much less is known regarding volatiles composition in the seed outgrowth mace and fruit and or roasting impact on its flavor. A volatile extraction method was applied for the first time to reveal for nutmeg distinct aroma using headspace solid-phase microextraction (SPME) coupled to mass spectrometry. A total of 53 volatiles were identified belonging to various classes viz., aromatic ethers, monoterpenes and sesquiterpenes. Compared to seeds and mace tissue, fruit was found more enriched in the key flavor “myristicin” 40%. In contrast, monoterpene hydrocarbons amounted as major volatile forms in seeds. Compared to nutmeg fruit, roasted seeds showed a distinct aroma composed of (E)-isoeugenol 16% and methoxyeugenol 11% not detected in fresh seed concurrent with lower anti-nutrient alkaloid levels. GC–MS was further utilized to localize primary metabolites (i.e., sugars and organic acids) and revealing for free sugars abundance in seeds at 47% versus enrichment of organic acids 58% viz. malic acid in fruit, and accounting for the later less palatable taste. This study provides the most comprehensive map for sensory metabolites distribution in nutmeg.

Spices and herbs are well-known for being rich in healthy bioactive metabolites. In recent years, interest in the fatty acid composition of different foods has greatly increased. Thus, the present study was designed to characterize the fatty acid composition of 34 widely used spices and herbs. Utilizing gas chromatography (GC) flame ionization detection (FID) and GC mass spectrometry (MS), we identified and quantified 18 fatty acids. This showed a significant variation among the studied spices and herbs. In general, oleic and linoleic acid dominate in seed spices, whereas palmitic, stearic, oleic, linoleic, and α-linolenic acids are the major constituents of herbs. Among the studied spices and herbs, the ratio of n−6/n−3 polyunsaturated fatty acids (PUFAs) was recorded to be in the range of 0.36 (oregano) to 85.99 (cumin), whereas the ratio of PUFAs/saturated fatty acids (SFAs) ranged from 0.17 (nutmeg) to 4.90 (cumin). Cumin, coriander, fennel, and dill seeds represent the healthiest fatty acid profile, based upon fat quality indices such as the ratio of hypocholesterolemic/hypercholesterolemic (h/H) fatty acids, the atherogenic index (AI), and the thrombogenic index (TI). All these seed spices belong to the Apiaceae family of plants, which are an exceptionally rich source of monounsaturated fatty acids (MUFAs) in the form of petroselinic acid (C18:1n12), with a very small amount of SFAs.

Aflatoxin contamination is one of the most important factors jeopardizing the quality of traditional Chinese health food (TCHF) during storage. Based on our previous work, we investigated the stability of chitosan (CH) films containing turmeric essential oil (TEO) and employed CH-TEO films as inner pouches, then stored them with inoculated Coix seed, nutmeg, and Ziziphi Spinosae Semen (ZSS). We found that the stability of CH-TEO was most affected by high temperature, and these pouches dramatically decreased aflatoxin accumulation and maintained levels of marker components of each TCHF. We found that glycerol tristearat in Coix seed and jujuboside A and spinosin in ZSS were negatively correlated with aflatoxin accumulation. After three months of storage with a CH-TEO pouch, we found little change in marker components contents, but observed that Coix seed had the relative lower sensory characteristics score. In addition, acute and 90-day subchronic toxicity test in Coix seed stored with the largest amount of TEO showed no significant signs of toxicity or treatment-related changes in animals. The present study is the first report on the study of a green, efficient, and low toxicity solution for aflatoxic contamination in TCHF, and provides strong support for its future use.

Nutmeg (Myristica fragrans Houtt) is a native plant of Banda Island known as the Spice Islands. Nutmeg fruit consists of the pericarp or rind, the seed kernel inside (nutmeg), and the nutmeg is a red lacy (aryl) covering the kernel (mace). Nutmeg pericarp contributing 80-85% of the total weight of the nutmeg fruit but its use is still not getting enough attention and a lot of it is wasted as agricultural waste which can pollute the environment. This is because the economic value is considered to be lower than the seeds and mace of nutmeg. This article aims to review the potential and oppurtunity benefits of nutmeg meat waste (pericarp) for human health and its application in functional foods. The method used in this paper is a literature review. The results show that, the pericarp has been reported to contain bioactive compounds similar to those of nutmeg and mace oil which have pharmacological values. Phytochemical compounds are beneficial to human health as anti-inflammatory, anti-diabetes, anti-microbial agents, antixidants, anti-depressants, anti-convulsants, and anti-cancer agents. Based on the composition, the pericarp is potentially used as a functional food to increase added value and reduce environmental pollution. In addition, the abundance of materials, relatively low prices, and the importance of healthy food for the health of the human provide opportunities for the development of functional foods based on bioactive compounds.

The effects of convection-oven precooking, frozen storage (&minus, 18 &deg, C/ two months) and four different reheating methods&mdash, namely, boiling, pan-roasting, convection oven and microwave oven on pH, color, texture, antioxidant activity and warmed-over flavor of beef meatballs were investigated. In this study, four kinds of beef meatballs were prepared: with added butylated hydroxyl toluene (0.02% BHT, M1), with nutmeg extract (0.02%, M2), with nutmeg powder (0.02%, M3) and control (no antioxidant). Addition of (0.02%) nutmeg extracts in beef meatballs M2 resulted in a significant (p &lt, 0.05) decrease in lipid and protein oxidation, hardness and gumminess values after convection oven precooking. Again, M2 reheated by microwave oven significantly (p &lt, 0.05) reduced cooking loss, gumminess, springiness, rancid flavor, saltiness and burnt taste and increased oxidative stability, redness and adhesiveness with the chewiness intensity and overall acceptability compared to control, M1 and M3. Conclusively, the addition of nutmeg extracts (0.02%) as a natural plant antioxidant to precooked beef meatballs can result in reduced lipid and protein oxidation levels, stabilized color and texture values and improved overall acceptance after reheated by microwave oven during two months of frozen storage.