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El maíz es una de las principales fuentes en la nutrición humana, y por su aporte nutrimental y contenido de azúcares fermentables es utilizado en la preparación de bebidas artesanales o tradicionales que pueden ser alcohólicas o no alcohólicas. La disponibilidad del maíz está determinada por la región, variación genética y condición geográfica donde se cultiva. Algunos tipos de maíz disponibles en las diferentes regiones poseen características propias como pueden ser tamaño, color, forma del grano y contenido de compuestos antioxidantes. Debido que existe poca información sobre formulación de bebidas y su comportamiento frente a tratamientos de conservación tradicional o tecnologías alternativas, el objetivo de este trabajo fue evaluar el efecto de un tratamiento alternativo de conservación con termosonicación y un tratamiento tradicional térmico de pasteurización a 85 °C sobre color, calidad microbiológica, parámetros fisicoquímicos y propiedades nutracéuticas de bebidas de maíz blanco y morado. Los resultados en las bebidas mostraron ligeras modificaciones de color y reducción de la cantidad de microorganismos, y no se observaron diferencias en las parámetros fisicoquímicos y compuestos fenólicos. En la bebida de maíz blanco se encontró mayor contenido de ácido ferúlico, mientras que en la bebida de maíz morado mostró un mayor contenido de ácido clorogénico, cafeico y mayores niveles de capacidad antioxidante. En conclusión, el tratamiento térmico tradicional de pasteurización resultó más eficiente que la termosonicación para la reducción de cantidad de microorganismos y no afectó las propiedades fisicoquímicas. Por otro lado, la capacidad antioxidante fue mayor en la bebida de maíz morado y esta se incrementó después del tratamiento térmico.

Corn stover, a corn harvest by-product, can be used to add value to feeds for farmed fish through their extracts, which are rich in phenolic compounds (PCs) and could confer antioxidant properties and possible health benefits to fish. In this study, PCs from corn husk extracts (CHE) were identified by ultra performance liquid chromatography-electrospray ionization-quadrupole time of fly-mass spectroscopy (UPLCESI-Q-ToF-MS/MS), and used to provide antioxidant potential to diets for Nile tilapia fingerlings. Three experimental diets for tilapia were formulated with 0, 100 and 200 mg PCs from CHE/kg feed and their antioxidant capacity was determined by DPPH• and ORAC assays. A total of 180 Nile tilapia fingerlings (0.35 ± 0.06 g) were fed for 14 days to determine the effect of experimental diets on feed intake and growth performance. PCs from CHE such as ferulic, p-coumaric and chlorogenic acids were the most abundant. The inclusion of PCs into tilapia diets increases linearly (p < 0.05) their antioxidant capacity (DPPH and ORAC). Growth performance and feed intake increased linearly (p < 0.05) as the level of CHE inclusion increased. Results suggest that the CHE can provide antioxidant potential to tilapia diets, promote growth performance and feed intake without toxic effects.RESUMENLa mazorca de maíz, un subproducto de la cosecha de maíz, puede usarse para agregar valor a los alimentos para peces de cultivo a través de sus extractos, que son ricos en compuestos fenólicos (PCs) y los cuales podrían conferir propiedades antioxidantes y posibles beneficios a la salud de los peces. En este estudio, las PCs de los extractos de cáscara de maíz (CHE) se identificaron mediante cromatografía líquida de ultra resolución-tiempo de vuelo con cuadrupolo acoplado a espectrometría de masas con ionización por electrospray (UPLC-ESI-Q-ToF-MS/MS), y se utilizaron para proporcionar potencial antioxidante a las dietas para alevines de tilapia del Nilo. Se formularon tres dietas experimentales para tilapia con 0, 100 y 200 mg de PCs de CHE/kg de alimento y su capacidad antioxidante se determinó mediante ensayos DPPH• y ORAC. Se alimentaron un total de 180 alevines de tilapia del Nilo (0.35 ± 0.06 g) durante 14 días para determinar el efecto de las dietas experimentales, sobre el consumo de alimento y el crecimiento. Los PCs de CHE como los ácidos ferúlico, p-cumárico y clorogénico fueron las más abundantes. La inclusión de PCs en las dietas para tilapia, aumenta linealmente (p

The production of marine foods is on the rise, and shrimp is one of the most widely consumed. As a result, a considerable amount of shrimp waste is generated, becoming a hazardous problem. Shrimp waste is a rich source of added-value components such as proteins, lipids, chitin, minerals, and carotenoids; however, new bioprocesses are needed to obtain these components. This work aimed to characterize the chemical and nutraceutical constituents from the liquor of shrimp waste recovered during a lactic acid fermentation process using the novel substrate sources whey and molasses. Our results showed that the lyophilized liquor is a rich source of proteins (25.40 ± 0.67%), carbohydrates (38.92 ± 0.19%), minerals (calcium and potassium), saturated fatty acids (palmitic, stearic, myristic and lauric acids), unsaturated fatty acids (oleic acid, linoleic, and palmitoleic acids), and astaxanthin (0.50 ± 0.02 µg astaxanthin/g). Moreover, fermentation is a bioprocess that allowed us to obtain antioxidants such as carotenoids with an antioxidant capacity of 154.43 ± 4.73 µM Trolox equivalent/g evaluated by the ABTS method. Our study showed that liquor from shrimp waste fermentation could be a source of nutraceutical constituents with pharmaceutical applications. However, further studies are needed to separate these added-value components from the liquor matrix.

Blueberry is a fruit consumed fresh and as an ingredient in formulations such as jam, juice, and wine. Wine is a widely consumed beverage and produced from different berries and fruits. Blueberry wine contains bio-compounds that could have a beneficial effect on health. Therefore, this study aimed to optimize blueberry wine by two response variables (total soluble solids and fermentation time) to obtain the highest alcohol percentage, pH, and lightness. In order to optimize the fermentation process, a central composite design was used. The optimized blueberry wine was obtained at total soluble solids of 25°Brix and fermentation time of 16 d. The optimized blueberry characteristics estimated were: alcohol percentage of 11.91%, pH of 2.98, and lightness (L*) of 26.22, and the optimized blueberry characteristics experimental were: alcohol percentage of 11.93±0.02%, pH of 2.97±0.01, and L* of 25.42±1.80. The optimized blueberry wine had a total phenolic content of 360.27±18.09 mg of gallic acid equivalents L-1, total anthocyanin content of 46.27±3.66 mg cyanidin-3 glucoside L-1, antioxidant capacity by ABTS and DDPH assays of 1,539.8±92.18 and 1,688.07±57.57 mM Trolox equivalent L-1, respectively. The results suggest that optimized blueberry wine can be considered a drink with potential health applications. Blueberry is a fruit consumed fresh and as an ingredient in formulations such as jam, juice, and wine. Wine is a widely consumed beverage and produced from different berries and fruits. Blueberry wine contains bio-compounds that could have a beneficial effect on health. Therefore, this study aimed to optimize blueberry wine by two response variables (total soluble solids and fermentation time) to obtain the highest alcohol percentage, pH, and lightness. In order to optimize the fermentation process, a central composite design was used. The optimized blueberry wine was obtained at total soluble solids of 25°Brix and fermentation time of 16 d. The optimized blueberry characteristics estimated were: alcohol percentage of 11.91%, pH of 2.98, and lightness (L*) of 26.22, and the optimized blueberry characteristics experimental were: alcohol percentage of 11.93±0.02%, pH of 2.97±0.01, and L* of 25.42±1.80. The optimized blueberry wine had a total phenolic content of 360.27±18.09 mg of gallic acid equivalents L-1, total anthocyanin content of 46.27±3.66 mg cyanidin-3 glucoside L-1, antioxidant capacity by ABTS and DDPH assays of 1,539.8±92.18 and 1,688.07±57.57 mM Trolox equivalent L-1, respectively. The results suggest that optimized blueberry wine can be considered a drink with potential health applications.

Pumpkin pulp is the main waste generated by pumpkin seed growers. This agro-industrial waste is a valuable source of bioactive compounds, especially carotenoids (β-carotene, α-carotene, and lutein), which exhibit a broad spectrum of health-promoting effects. In this study, vegetable oils (canola, corn, and soybean oil) were used as green solvent alternatives to conventional organic solvents for carotenoid extraction from dried pumpkin pulp (DPP) waste. The highest carotenoid extraction yield (CEY) was obtained with canola oil, at a 1:10 DPP/oil ratio. Response surface methodology (RSM) was used to optimize the extraction process parameters (temperature, time, and stirring rate) through a Box-Behnken design (BBD) maximizing CEY in canola oil. The extraction temperature and stirring rate were found to have a significant linear and quadratic effect, respectively, on CEY. Optimum conditions were achieved at 21.8 min, 250 rpm, and 60°C. Under these optimized conditions, the estimated value for CEY was 378.1 µg β-carotene equivalents/g of DPP, corresponding to 61.6% of the total carotenoid content present in the DPP. In contrast, the observed experimental value was 373.2 µg β-carotene equivalents/g of DPP (61.2%). The experimental value was very close to the estimated value, which verifies the model's adequacy and fit. This study shows an alternative method to extract carotenoids from DPP with canola oil, obtaining an oil naturally enriched with carotenoids that could be used as a potential functional ingredient in the development of food, cosmetics, and medicinal products. PRACTICAL APPLICATION: Pumpkin by-products are a potential carotenoid source. Vegetable oil can be used as an alternative solvent for carotenoid extraction from pumpkin residues to obtain an enriched carotenoid oil that can be used to formulate food products.

Four experimental diets were formulated with 50, 100, 150 and 200 mg of phenolic compounds (PCs) kg-1 feed, derived from mango peel. The control diet did not contain PCs. A total of 120 male zebrafish (Danio rerio) (average weight: 166 mg) were fed for eight weeks to assess the ability of PCs to prevent lipid peroxidation and enhance antioxidant status. Growth performance was calculated at the end of the experimental trial. Lipid peroxidations in muscle and antioxidant enzyme activity in the liver were evaluated at the end of the experiment. There was no significant difference in growth performance among treatments. Malondialdehyde (MDA) levels in muscle were significantly lower in fish fed diets containing 50 and 100 mg of PCs per kg of feed. Incorporation of PCs into zebrafish diet did not have any significant effects on glutathione peroxidase and superoxide dismutase activity. However, catalase (CAT) activity increased significantly in fish with diets containing 100, 150 and 200 mg of PCs kg-1 feed. These results suggest a potential protective effect against in vivo lipid peroxidation and CAT-modulating effects.

Mexico is the world’s largest producer, exporter, and consumer of avocados. “Hass” avocado is the most commercialized cultivar, while the native Mexican avocado varieties have been displaced. Thus, studies regarding their chemical and nutraceutical characterization are scarce. We studied the total lipid content, fatty acid profile, carotenoid content, and free radical-scavenging activity of the fruit pulp from 12 accessions of the native Mexican avocado (Persea americana var. drymifolia). The results show that the chemical and nutraceutical characteristics depend on the accession type. The total lipid content ranged from 13.22 to 23.41%. The major saturated fatty acid in all the avocado accessions was palmitic acid (15.54–22.68%). Monounsaturated fatty acids, like oleic (56.58–74.19%), linoleic (5.62–16.85%) and linolenic (0.63–2.85%) acids were the most abundant unsaturated fatty acids in all the native Mexican avocado accessions. The total carotenoid content (1.57 to 7.83 mg eq. of β-carotene 100 g-1 fresh weight) and the free radical-scavenging activity (7.58–88.62 mMol trolox equivalent 100 g−1 fresh weight) also varied significantly (p

The aim of this study was the characterization of chromatic, nutritional and nutraceutical properties of fifteen native maize genotypes of the races: conico norteno, elotes conicos, raton, conico norteno × elotes conicos, raton × elotes conicos, Celaya × elotes conicos, elotes conicos × conico norteno from the northeast of Mexico. The results showed that the values of the color parameters L*, C* and h in genotypes range from 25.13 to 63.64, 2.41 to 33.58 and 14.36 to 359.19, respectively. The percentage of nutritional component varied from carbohydrates (71.30–74.88), protein (9.72–12.57) and moisture (7.98–9.67). The samples were rich in P, K, Mg, Zn and Fe. The total phenols content (free + bound) ranged from 349.39 to 485.71 mgGAE/100 g, showing bound fractions higher content than free fractions. The total flavonoids (free + bound) were from 24.00 to 105.75 mgCE/100 g, and for eight of the genotypes the bound fractions were higher than free fractions. Condensed tannins were only found in free fractions with values ranging from 33.70 to 158.55 mgCE/100 g. Total anthocyanins ranged from 1.38 to 74.52 mgC3GE/100 g. In HPLC analysis, ferulic acid (103.34–164.90 mg/100 g) was the main phenolic compound followed by coumaric acid (5.57–15.52 mg/100 g) and they were only identified in bound extracts. Finally, the antioxidant capacity (free + bound) expressed in µmolTE/100 g was from 1127.70 to 1875.70, 2826.90 to 4263.90, 730.94 to 1340.65 and 3484.80 to 5592.60 in DPPH, ABTS, FRAP and ORAC, respectively, and IC50 to DPPH and ABTS were expressed in mg/mL. In conclusion, significant correlation was found between chromatic versus antioxidant capacity, and anthocyanin content. In addition, higher phenolic content were found in bound extracts, and a genetic diversity was observed between maize genotypes.

Blue maize owes its coloration to a high anthocyanin content, and is therefore a promising source of unstable bioactive compounds. Spray-drying microencapsulation (SDM) can maintain the integrity of labile compounds during storage and digestion. We microencapsulated blue maize polyphenols by SDM in two matrices, maltodextrin (MD) and maltodextrin-pectin (MP) mixture. Morphology, degradation kinetics during storage and release were analyzed. Particle size ranged from 3 to 5 µm, with spherical morphology and surface depressions. The compounds and antioxidant activity had longer half-life in the MP matrix. Both matrices showed near complete release under simulated digestion (~ 95%), the majority of the compounds in the MP matrix were released during the intestinal phase, while those in the MD matrix were released during the gastric phase. These results suggest that microencapsulating blue maize polyphenols allows their incorporation into other products, and the addition of pectin improves the protective properties of MD, possibly due to its lower reactivity.

Feruloylated arabinoxylans were obtained from nixtamalized maize bran under alkaline conditions (0.5 N NaOH) at different times (2 h, 4 h and 6 h). They were analyzed for neutral sugars composition, dietary fiber and ferulic acids content, as well as the antioxidant capacity, to establish if they can be considered as soluble antioxidant dietary fiber. The yields of the arabinoxylans following alkaline extraction treatments were 4.89%, 8.23% and 7.17% for 2 h, 4 h and 6 h, respectively. The purity of arabinoxylans ranged from 55.58 to 61.16%, while arabinose to xylose (Ara/Xyl) ratio ranged from 0.82 to 0.87 which indicated that all arabinoxylans had a moderately branched structure. The soluble dietary fiber accounted for more than 85% of the chemical composition of feruloylated arabinoxylans. Monomeric and oligomeric forms of ferulic acid were influenced by the alkali extraction time. The monomeric form was the main phenolic acid in feruloylated arabinoxylans (77.05–86.97%), followed by dimers (11.57–14.20%), and trimer (0.93–9.36%). Total phenols ranged from 9.01 to 6.48 mg FAE/g, while antioxidant capacity ranged from 29.49 to 31.69 µmol TE/g, 16.60 to 21.27 µmol TE/g, 39.23 to 58.33 µmol TE/g and 17.03 to 60.65 µmol TE/g in DPPH, ABTS, FRAP and ORAC methods, respectively. The phenol content and antioxidant capacity were in the order: 2 h extract > 4 h extract > 6 h extract and in accordance to the ferulic acid content. The results indicated that alkali extracted feruloylated arabinoxylans obtained from nixtamalized maize bran byproduct are synonymous with soluble antioxidant dietary fiber.

Antioxidant capacities of hydrophilic (phenolic compounds; H-MPE) and lipophilic (carotenoids; L-MPE) antioxidants from mango peel by ABTS and DPPH assays were determined. The ability of H-MPE and L-MPE to inhibit fish oil peroxidation by the formation of malondialdehyde (MDA) was measured and compared against synthetic antioxidant butylated hydroxytoluene (BHT) to study their antioxidant potential in the stabilization of omega-3 polyunsaturated fatty acids contained in the fish oil. H-MPE and L-MPE showed significantly higher antioxidant capacity than BHT (P

The fruit of 18 Solanum lycopersicum var. cerasiforme accessions from Mexico were evaluated for total phenolics (TP) by the Folin-Ciocalteau assay, phenolic profiles by high performance liquid chromatography-diode array detection-mass spectrometry (HPLC-DAD-MS), antioxidant activity (AoxA) by 2,2´-azino-bis(3-ethylbenzothiazolin)-6-sulfonic acid (ABTS), 2,2-diphenil-1-pycrilhydrazyl (DPPH), and oxygen radical absorbance capacity (ORAC), and antimutagenic activity (AmuA) by the Ames assay. TP was measured as Gallic Acid Equivalents (GAE) and the AoxA as Trolox Equivalents (TE). TP varied from 37 to 86 mg GAE 100 g−1 fresh weight (fw). The AoxA by ABTS (568-1187 µmol TE 100 g−1 fw) and DPPH (157-350 µmol TE 100 g−1 fw) correlated with TP and the levels of caffeoylquinic acids and rutin. The AmuA did not correlate with the levels of phenolics. Some accessions had higher AoxA and AmuA than those reported for commercial cultivars and also showed high levels of caffeoylquinic acids and rutin; thus, their consumption could have good health promoting effects.

Lactic acid fermentation increases the bioactive properties of shrimp waste. Astaxanthin is the principal carotenoid present in shrimp waste, which can be found esterified in the liquid fraction (liquor) after its lactic acid fermentation. Supercritical CO2 technology has been proposed as a green alternative to obtain astaxanthin from fermented shrimp waste. This study aimed to optimize astaxanthin extraction by supercritical CO2 technology from fermented liquor of shrimp waste and study bioaccessibility using simulated gastrointestinal digestion (GD) of the optimized extract. A Box–Behnken design with three variables (pressure, temperature, and flow rate) was used to optimize the supercritical CO2 extraction. The optimized CO2 extract was obtained at 300 bar, 60 °C, and 6 mL/min, and the estimated characteristics showed a predictive extraction yield of 11.17%, antioxidant capacity of 1.965 mmol of Trolox equivalent (TE)/g, and astaxanthin concentration of 0.6353 µg/g. The experiment with optimal conditions performed to validate the predicted values showed an extraction yield of 12.62%, an antioxidant capacity of 1.784 mmol TE/g, and an astaxanthin concentration of 0.52 µg/g. The astaxanthin concentration decreased, and the antioxidant capacity of the optimized extract increased during gastrointestinal digestion. In conclusion, our optimized supercritical CO2 process is suitable for obtaining astaxanthin from shrimp by-products after lactic acid fermentation.

Low temperature (LT) and ultraviolet-B (UV-B) radiation are abiotic factors that cause plant stress and limit crop production because of their detrimental effects on photosynthetic components. There is evidence of chlorophyll and carotenoid degradation by LT and UV-B radiation, as well as the production of phenolic compounds as defense components against these stress factors; however, there is scarce information about the interactions between LT and UV-B radiation. Therefore, in this study, the contents of chlorophyll, carotenoids, and phenolic compounds were analyzed in response to LT, UV-B radiation and the combination of both (LT + UV-B) in leaves of bell pepper plants. The LT + UV-B condition produced the greater degradation of chlorophyll in the bell pepper leaves and the higher accumulation of carotenoids, chlorogenic acid and the flavonoids apigenin-7- O -glucoside (A-7-G) and luteolin-7- O -glucoside (L-7-G) compared to the LT and UV-B conditions applied separately. UV-B radiation induced a higher total flavonoid concentration than LT, but the highest flavonoid concentration was observed in the leaves exposed to LT + UV-B. The higher accumulation of chlorogenic acid and A-7-G biosynthesis in leaves exposed to LT than control, indicate a higher resistance of plants to UV-B radiation damage because chlorogenic acid and A-7-G both have high UV-B absorbance capacities. However, a higher concentration of L-7-G, respect to A-7-G, in leaves exposed to UV-B and LT + UV-B indicates a higher necessity to quench ROS, because L-7-G has a higher antioxidant capacity than A-7-G. The increment of carotenoid and L-7-G concentrations in bell pepper plants exposed to LT and UV-B radiation stress displayed a high correlation with the raise of total reducing capacity in their leaves.

Mango bark is an important agro-industrial residue from mango pruning. In traditional medicine, the aqueous extract from mango bark (MBE) has been used in ethnomedicine for the treatment of many diseases. However, there is scarce information using cellular models to evaluate the potential use of this plant material for human consumption. In this study, the phenolic content from the MBE from four varieties (Kent, Keitt, Ataulfo and Tommy Atkins) was analyzed by high-performance liquid chromatography coupled to photodiode array detector (HPLC-DAD) and liquid chromatography coupled with time-of-flight mass spectrometry (LC/MS-TOF). Additionally, the cellular antioxidant activity of the MBE from the four mango varieties were compared. Finally, the intestinal permeability of the main polyphenols found in the MBE (mangiferin and gallic acid) was evaluated. RESULTS Mangiferin and gallic acid were the main constituents in the MBE from the four mango varieties. Furthermore, the Ataulfo variety showed the highest cellular antioxidant activity (67%) at the concentration of 100 µg mL?1. The intestinal permeability of mangiferin present in the bark extracts was 3- to 4.8-fold higher than those of mangiferin as standard, whereas the intestinal permeability of gallic acid varied among the tested extracts. CONCLUSION MBE has the potential to exert antioxidant activity at the cellular level and can have an impact on human health. It may also be a good source for the extraction of polyphenols mainly mangiferin. ? 2019 Society of Chemical Industry

Prebiotics are nondigestible food components that have an impact on gut microbiota composition and activity, which in turn results in the improvement of health conditions. Nowadays, the production of prebiotics from agro-industrial by-products is under investigation. In this regard, polysaccharides are usually found in these sources and their potential use as prebiotics has been studied recently since these compounds act as substrates for the human gut microbiota, and they have the potential to modulate its composition through many mechanisms. Additionally, the use of agricultural by-products is advantageous because it is a cheap and abundantly available material. This review focuses on the recent scientific literature regarding the prebiotic properties of polysaccharides from agro-industrial by-products. PRACTICAL APPLICATIONS: Currently, the maintenance of gut homeostasis is a target for the improvement of human health. This review can broaden the perspective on the utilization of agro-industrial by-products that can compete in the market with the commercial ones or act as a source for new food ingredients.

espanolSe evaluo el contenido de compuestos fenolicos solubles de tipo flavonoide en terminos de flavonoides totales (AlCl3-NaNO2-NaOH), taninos condensados (vainillina-H2SO4) y antocianinas totales (absorbancia del extracto a 535 nm, pH=1). Ademas se evaluo tambien la capacidad antioxidante basada en la reduccion de la absorbancia de los radicales DPPH (2,2-difenil-1-picrilhidrazilo) y ABTS (acido-2,2-azinobis-( 3-etilbenzotiazolina)-6-sulfonico)) en cinco genotipos criollos pigmentados de maiz: Ejido La Soledad Arramberri (ELSA), Ejido Refugio Zaragoza (ERZ), La Siberia Zaragoza (LSZ), Morado Oaxaca (MO) y Tepozanes II (TZII). El contenido de flavonoides totales, taninos condensados y antocianinas totales fueron de 5709 a 7418 miligramos equivalentes catequina por kilogramo (mgECat/kg), de 798 a 1322 mgECat/ kg y de 338 a 513 miligramos equivalentes cianidina-3-glucosido por kilogramo (mgEC3G/kg), respectivamente. En cuanto a la capacidad antioxidante, en terminos de equivalentes Trolox los niveles fueron de 23,00 a 27,88 milimoles equivalentes Trolox por kilogramo (mmolET/kg) y de 14,97 a 18,12 mmolET/kg en DPPH y ABTS respectivamente, mientras que en terminos de porcentaje de inhibicion los niveles fueron de 17,44 a 21,31 por ciento de inhibicion (%Inh) y de 22,97 a 27,22 %Inh en los ensayos DPPH y ABTS respectivamente. El genotipo TZII presento los niveles mas altos en las evaluaciones de flavonoides totales y capacidad antioxidante DPPH y ABTS, mientras que los genotipos ELSA y MO presentaron el mayor contenido de taninos condensados y antocianinas totales, respectivamente EnglishThe content of flavonoid type soluble phenolic compounds in terms of total flavonoids (AlCl3-NaNO2- NaOH), condensed tannins (vanillin-H2SO4) and total anthocyanins (extract absorbance at 535 nm, pH=1) was evaluated. In addition the antioxidant capacity was also evaluated based on the absorbance reduction of the DPPH (2,2-diphenyl-1-picrylhydrazyl) and ABTS (2,2 -Azino-bis(3-ethylbenzothiazoline-6- sulfonic acid)) reagents in five creole pigmented maize genotypes: Ejido La Soledad Arramberri (ELSA), Ejido Refugio Zaragoza (ERZ), La Siberia Zaragoza (LSZ), Morado Oaxaca (MO) y Tepozanes II (TZII). The content of total flavonoids, condensed tannins and total anthocyanins ranged from 5709 to 7418 milligrams catechins equivalents per kilogram (mgCatE/kg), from 798 to 1322 mgCatE/kg and from 338 to 513 milligrams cyaniding-3-glucoside per kilogram (mgC3GE/kg), respectively. Regarding to the antioxidant capacity, in terms of Trolox equivalents levels were from 23.00 to 27.88 millimoles Trolox equivalents per kilogram (mmolTE/kg) and 14.97 to 18.12 mmolTE/kg in DPPH and ABTS respectively, while in terms of inhibition percentage levels were from 17.44 to 21.31 percentage inhibition (%Inh) and from 22.97 to 27.22 %Inh in DPPH y ABTS respectively. Genotype TZII showed the highest levels in the total flavonoids and DPPH and ABTS antioxidant capacity, while ELSA and MO genotypes showed the highest levels of condensed tannins and total anthocyanins, respectively

Blue maize is an excellent source of bioactive components such as phenolic acids and anthocyanins but when it is processed for human consumption, these compounds decrease considerably. Therefore, blue maize could be directed to produce nutraceutical extracts. The aim of this study was to evaluate the relation between anthocyanins composition of acidified and non-acidified extracts from native and hybrid blue maize genotypes and their antiproliferative effect in mammary (MCF7), liver (HepG2), colon (Caco2 and HT29) and prostate (PC3) cancer cells. The most abundant phenolic acid was ferulic acid. Nine anthocyanins were quantified in the extracts, being Cy3-Glu the most abundant. Acylated forms were also obtained in high abundance depending of the extraction method. An extract concentration range of 4.31 to 7.23 mg/mL inhibited by 50 % the growth of untransformed cells NIH3T3. Antiproliferative effect on PC3, Caco2, HepG2 and MCF7 cancer cells of acidified extracts from hybrid blue maize was larger than the observed using non-acidified extracts. Among the nine compounds that were quantified in the extracts tested, CyMalGlu I showed the strongest correlation with the reduction of cell viability in Caco2 (−0.876), HepG2 (−0.813), MCF7 (−0.765) and PC3 (−0.894). No significant correlation or differences in antiproliferative effect on HT29 was found among the extracts. The method of extraction of maize anthocyanins must be selected to obtain a high yield of CyMalGlu I more than only Cy3-Glu since acylation affects the inhibition of cancer cell growth.

Free and bound phenolics, antioxidant capacity and anthocyanins content of five elite blue maize hybrids and two native blue varieties grown in Mexico highlands were evaluated. Phenolic content of free and bound fractions was found from 1037 to 1488 and from 9064 to 11,987 mg gallic acid/kg, respectively. The free phenolic fraction of hybrid maize showed 3.7-fold higher hydrophilic antioxidant capacity than landrace counterparts. The highest anthocyanin content was found in hybrid 503 × 67 (1052 mg Cy3Glu/kg), which represented 73.1% of the free phenolics. Results indicated that elite blue maize hybrids could be an important source of antioxidant compounds with potential for either food or nutraceutical industries.

Phenolic compounds are ubiquitous in plant-based foods. High dietary intake of fruits, vegetables and cereals is related to a decreased rate in chronic diseases. Phenolic compounds are thought to be responsible, at least in part, for those health effects. Nonetheless, phenolic compounds bioaccessibility and biotransformation is often not considered in these studies; thus, a precise mechanism of action of phenolic compounds is not known. In this review we aim to present a comprehensive knowledge of the metabolic processes through which phenolic compounds go after intake. Los compuestos fenólicos son ubicuos en alimentos de origen vegetal. La alta ingesta de frutas, vegetales y cereales está relacionada con un bajo índice en padecimientos crónicos. Se cree que los compuestos fenólicos son, en parte, responsables de este efecto benéfico. Sin embargo, la bioaccesibilidad y biotransformación de los compuestos fenólicos generalmente no es considerada en este tipo de estudios. Por lo tanto, no se ha podido obtener un mecanismo de acción de los compuestos fenólicos. En este trabajo, presentamos una revisión de literatura de los procesos metabólicos a través de los cuales los compuestos fenólicos son sometidos después de ser ingeridos.

The aim of this study was to evaluate the effect of the variety and calcium salts on quality of fresh-cut pear tomatoes. The study was divided into two experiments: the first was conducted to determine which genotype was more feasible for making fresh-cut tomato products (Cuauhtemoc vs IntenseTM). For that purpose, firmness, color, pH, acidity and total soluble solids were evaluated. The second experiment was conducted to determine the effect of 3 % calcium chloride and 3 % calcium lactate on the selected fresh-cut tomato genotype, IntenseTM. The same variables of the previous experiment were evaluated. In experiment I, the genotype IntenseTM was 42 % firmer than the Cuauhtemoc genotype, and also it showed a brighter and more defined color. From experiment II, 3 % calcium chloride showed the best results in terms of firmness, with 50 and 100 % firmer fresh-cut tomatoes as compared to 3 % calcium lactate and control samples, respectively. No significant differences in color and chemical variables were observed. The IntenseTM tomato genotype provides better quality, and when combined with a calcium salt, it makes a better fresh-cut product with a longer shelf life. ADDITIONAL

El cultivo intensivo de peces es una estrategia económicamente importante para producir alimento. Sin embargo, las prácticas de cultivo intensivo generan estrés oxidativo e inmunosupresión, lo que ocasiona pérdidas de la calidad del espécimen y aumento en la mortalidad. Para contrarrestar estos efectos, se ha optado por la administración de vegetales como fuente de polifenoles con propiedades antioxidantes e inmunoestimulantes en peces carnívoros de cultivo. El objetivo de este trabajo fue describir los efectos de los polifenoles de origen vegetal como antioxidantes e inmunoestimulantes en peces carnívoros, y promover su uso como ingredientes funcionales en la acuicultura. Los vegetales como fuente de polifenoles tienen la capacidad de mejorar los sistemas de defensa inmune y antioxidante de las especies analiza- das, con un tejido de mejor calidad nutricional y un mayor contenido endógeno de antioxidantes. No obstante, las propiedades biológicas de los polifenoles dependen del tipo y concentración en el vegetal, de la dosis y el tiempo de administración, así como de la matriz alimentaria, la cual determina la bioaccesibilidad y biodisponibilidad de los polifenoles en el organismo. Es escasa la información generada sobre el efecto de los polifenoles en la calidad post mortem, por lo que se deben realizar más estudios.