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1. Waterborne fluorescent dual anti-counterfeiting ink based on Yb/Er-carbon quantum dots grafted with dialdehyde nano-fibrillated cellulose.

Author

Li P, Zeng J, Wang B, Cheng Z, Xu J, Gao W, and Chen K

Subjects
Cellulose chemistry, Erbium chemistry, Water, Ytterbium chemistry, Carbon chemistry, Cellulose analogs & derivatives, Counterfeit Drugs chemistry, Fluorescence, Fraud prevention & control, Ink, Quantum Dots
Abstract

Nanofibrillated cellulose (NFC) is becoming popular in the field of anti-counterfeiting material due to its favorable biocompatibility, renewability, and easy modification properties, which give it great potentials as carrier of carbon quantum dots (CQDs). Herein, we report an effective method to fabricate Yb and Er doped CQDs grafted onto dialdehyde NFC (DANFC). Owning to special rheological properties of NFC, a waterborne fluorescent dual anti-counterfeiting ink was rationally designed and successfully prepared by adding NFC to waterborne ink to form a stable network structure and increase the thixotropy and yield stress. The resulting CQDs exhibited both photoluminescence (PL) and up-conversion luminescence (UCPL), emitting blue and green fluorescence at excitation wavelengths of 370 and 980 nm, respectively. The study provides a novel method to prepare the waterborne fluorescent dual anti-counterfeiting ink based on Yb and Er doped CQDs/DANFC composites, which provides a reference for its application in printing and packaging industry., (Copyright © 2020 Elsevier Ltd. All rights reserved.)

Published
2020
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2. Fluorescent strip sensor for rapid determination of toxinsElectronic supplementary information (ESI) available: Detailed information of the whole experimental procedure, the characterization of QD–aptamer conjugation and factors. See DOI: 10.1039/c0cc04032k

Author

WangThese authors contributed equally to this paper., Libing, Chen, Wei, Ma, Wenwei, Liu, Liqinag, Ma, Wei, Zhao, Yuan, Zhu, Yingyue, Xu, Liguang, Kuang, Hua, and Xu, Chuanlai

Subjects
*OCHRATOXINS, *FLUORESCENCE, *EXPERIMENTS, *QUANTUM dots, *NANOPARTICLES, *COLLOIDAL gold, *SOLUTION (Chemistry)
Abstract

Here, we report a simple fluorescent strip sensor based on aptamer–quantum dots technology that can meet toxin monitoring demands using ochratoxin A (OTA) as a model toxin. The limit of the detection (LOD) for the fluorescent strip was 1.9 ng mL−1, while the time needed for the detection is only 10 min; this conforms to the standards of World Health Organization (WHO) or better. Overall functional parameters are also better than the analogous characteristics of gold nanoparticle strips.High selectivity was maintained as well, making them suitable for the samples with complex solution composition. [ABSTRACT FROM AUTHOR]

Published
2011

3. In Vitro and Ex Vivo Evaluation of Smart Infra-Red Fluorescent Caspase-3 Probes for Molecular Imaging of Cardiovascular Apoptosis

Author

Bruno Delest, Vincent Richard, Marc Massonneau, Manuelle Debunne, Jean-Paul Henry, Christian Thuillez, Anthony Romieu, Christophe Portal, Françoise Lallemand, Heidi Ligeret, Pierre-Yves Renard, Ebba Brakenhielm, Pauline Noack, Pharmacologie des Dysfonctionnements Endotheliaux et Myocardiques, Institut National de la Santé et de la Recherche Médicale (INSERM)-Université de Rouen Normandie (UNIROUEN), Normandie Université (NU)-Normandie Université (NU), QUantitative Imaging in Drug Development [Saint-Etienne du Rouvray] (QUIDD / Technopôle du Madrillet), Technopôle du Madrillet, Chimie Organique et Bioorganique : Réactivité et Analyse (COBRA), Institut Normand de Chimie Moléculaire Médicinale et Macromoléculaire (INC3M), Institut de Chimie du CNRS (INC)-École Nationale Supérieure d'Ingénieurs de Caen (ENSICAEN), Normandie Université (NU)-Normandie Université (NU)-Institut national des sciences appliquées Rouen Normandie (INSA Rouen Normandie), Institut National des Sciences Appliquées (INSA)-Normandie Université (NU)-Institut National des Sciences Appliquées (INSA)-Université Le Havre Normandie (ULH), Normandie Université (NU)-Université de Rouen Normandie (UNIROUEN), Normandie Université (NU)-Centre National de la Recherche Scientifique (CNRS)-Université de Caen Normandie (UNICAEN), Normandie Université (NU)-Institut de Chimie du CNRS (INC)-École Nationale Supérieure d'Ingénieurs de Caen (ENSICAEN), Normandie Université (NU)-Centre National de la Recherche Scientifique (CNRS)-Institut de Chimie Organique Fine (IRCOF), Université de Rouen Normandie (UNIROUEN), Institut National des Sciences Appliquées (INSA)-Normandie Université (NU)-Institut National des Sciences Appliquées (INSA)-Centre National de la Recherche Scientifique (CNRS)-Centre National de la Recherche Scientifique (CNRS), This paper was supported by La Région Haute Normandie via the CRUNCh program (CPER 2007–2013) and IUF (Institut Universitaire de France)., Brakenhielm, Ebba, Normandie Université (NU)-Normandie Université (NU)-Institut National de la Santé et de la Recherche Médicale (INSERM), Institut de Chimie Organique Fine (IRCOF), Institut National des Sciences Appliquées (INSA)-Normandie Université (NU)-Institut National des Sciences Appliquées (INSA)-Centre National de la Recherche Scientifique (CNRS)-Université de Rouen Normandie (UNIROUEN), Institut National des Sciences Appliquées (INSA)-Normandie Université (NU)-Institut National des Sciences Appliquées (INSA)-Centre National de la Recherche Scientifique (CNRS)-Institut Normand de Chimie Moléculaire Médicinale et Macromoléculaire (INC3M), Université de Caen Normandie (UNICAEN), Normandie Université (NU)-Normandie Université (NU)-École Nationale Supérieure d'Ingénieurs de Caen (ENSICAEN), Normandie Université (NU)-Université Le Havre Normandie (ULH), Normandie Université (NU)-Institut national des sciences appliquées Rouen Normandie (INSA Rouen Normandie), Institut National des Sciences Appliquées (INSA)-Normandie Université (NU)-Institut National des Sciences Appliquées (INSA)-Institut de Chimie du CNRS (INC)-Centre National de la Recherche Scientifique (CNRS)-Université de Caen Normandie (UNICAEN), Normandie Université (NU)-École Nationale Supérieure d'Ingénieurs de Caen (ENSICAEN), and Normandie Université (NU)-Institut de Chimie du CNRS (INC)-Centre National de la Recherche Scientifique (CNRS)-Centre National de la Recherche Scientifique (CNRS)

Subjects
Pathology, medicine.medical_specialty, [SDV.BIO]Life Sciences [q-bio]/Biotechnology, [SDV.IMM] Life Sciences [q-bio]/Immunology, Article Subject, [SDV.MHEP.PHY] Life Sciences [q-bio]/Human health and pathology/Tissues and Organs [q-bio.TO], Caspase 3, Flow cytometry, 03 medical and health sciences, 0302 clinical medicine, [SDV.MHEP.CSC]Life Sciences [q-bio]/Human health and pathology/Cardiology and cardiovascular system, Annexin, medicine, [SDV.MHEP.PHY]Life Sciences [q-bio]/Human health and pathology/Tissues and Organs [q-bio.TO], Radiology, Nuclear Medicine and imaging, 030304 developmental biology, 0303 health sciences, medicine.diagnostic_test, Chemistry, [SDV.SP]Life Sciences [q-bio]/Pharmaceutical sciences, Fluorescence, In vitro, 3. Good health, Cell biology, [SDV.MHEP.CSC] Life Sciences [q-bio]/Human health and pathology/Cardiology and cardiovascular system, [SDV.BIO] Life Sciences [q-bio]/Biotechnology, [SDV.SP] Life Sciences [q-bio]/Pharmaceutical sciences, Apoptosis, 030220 oncology & carcinogenesis, [SDV.IMM]Life Sciences [q-bio]/Immunology, Molecular imaging, Ex vivo, Research Article
Abstract

Purpose. The aim of this paper is to develop new optical bioprobes for the imaging of apoptosis.Procedure. We developed quenched near-infrared probes which become fluorescent upon cleavage by caspase-3, the key regulatory enzyme of apoptosis.Results. Probes were shown to be selectively cleaved by recombinant caspase-3. Apoptosis of cultured endothelial cells was associated with an increased fluorescent signal for the cleaved probes, which colocalized with caspase-3 and was reduced by the addition of a caspase-3 inhibitor. Flow cytometry demonstrated a similar profile between the cleaved probes and annexin V.Ex vivoexperiments showed that sections of hearts obtained from mice treated with the proapoptotic drug doxorubicin displayed an increase in the fluorescent signal for the cleaved probes, which was reduced by a caspase-3 inhibitor.Conclusion. We demonstrated the capacity of these novel probes to detect apoptosis by optical imagingin vitroandex vivo.

Published
2011