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In Vitro and Ex Vivo Evaluation of Smart Infra-Red Fluorescent Caspase-3 Probes for Molecular Imaging of Cardiovascular Apoptosis
- Source :
- International Journal of Molecular Imaging, International Journal of Molecular Imaging, Hindawi Publishing Corporation, 2011, 2011, pp.413290. ⟨10.1155/2011/413290⟩, International Journal of Molecular Imaging, 2011, 2011, pp.413290. ⟨10.1155/2011/413290⟩
- Publication Year :
- 2011
- Publisher :
- Hindawi Publishing Corporation, 2011.
-
Abstract
- Purpose. The aim of this paper is to develop new optical bioprobes for the imaging of apoptosis.Procedure. We developed quenched near-infrared probes which become fluorescent upon cleavage by caspase-3, the key regulatory enzyme of apoptosis.Results. Probes were shown to be selectively cleaved by recombinant caspase-3. Apoptosis of cultured endothelial cells was associated with an increased fluorescent signal for the cleaved probes, which colocalized with caspase-3 and was reduced by the addition of a caspase-3 inhibitor. Flow cytometry demonstrated a similar profile between the cleaved probes and annexin V.Ex vivoexperiments showed that sections of hearts obtained from mice treated with the proapoptotic drug doxorubicin displayed an increase in the fluorescent signal for the cleaved probes, which was reduced by a caspase-3 inhibitor.Conclusion. We demonstrated the capacity of these novel probes to detect apoptosis by optical imagingin vitroandex vivo.
- Subjects :
- Pathology
medicine.medical_specialty
[SDV.BIO]Life Sciences [q-bio]/Biotechnology
[SDV.IMM] Life Sciences [q-bio]/Immunology
Article Subject
[SDV.MHEP.PHY] Life Sciences [q-bio]/Human health and pathology/Tissues and Organs [q-bio.TO]
Caspase 3
Flow cytometry
03 medical and health sciences
0302 clinical medicine
[SDV.MHEP.CSC]Life Sciences [q-bio]/Human health and pathology/Cardiology and cardiovascular system
Annexin
medicine
[SDV.MHEP.PHY]Life Sciences [q-bio]/Human health and pathology/Tissues and Organs [q-bio.TO]
Radiology, Nuclear Medicine and imaging
030304 developmental biology
0303 health sciences
medicine.diagnostic_test
Chemistry
[SDV.SP]Life Sciences [q-bio]/Pharmaceutical sciences
Fluorescence
In vitro
3. Good health
Cell biology
[SDV.MHEP.CSC] Life Sciences [q-bio]/Human health and pathology/Cardiology and cardiovascular system
[SDV.BIO] Life Sciences [q-bio]/Biotechnology
[SDV.SP] Life Sciences [q-bio]/Pharmaceutical sciences
Apoptosis
030220 oncology & carcinogenesis
[SDV.IMM]Life Sciences [q-bio]/Immunology
Molecular imaging
Ex vivo
Research Article
Subjects
Details
- Language :
- English
- ISSN :
- 20901720 and 20901712
- Volume :
- 2011
- Database :
- OpenAIRE
- Journal :
- International Journal of Molecular Imaging
- Accession number :
- edsair.doi.dedup.....733d54a377e2ec39a54f390b7a9afa28