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Abstract: A full factorial design was conducted to investigate the combined effects of temperatures and initial pH on fermentative hydrogen production by mixed cultures in batch tests. The experimental results showed that the modified Logistic model can be used to describe the progress of cumulative hydrogen production in the batch tests of this study. The modified Ratkowsky model can be used to describe the combined effects of the temperatures and initial pH on the substrate degradation efficiency, hydrogen yield and average hydrogen production rate. The temperatures and initial pH had interactive impact on fermentative hydrogen production. The maximum substrate degradation efficiency, the maximum hydrogen yield and the maximum average hydrogen production rate was predicted at the temperature of 37.8°C and the initial pH of 7.1, 37.4°C and 6.9, and 38.2°C and 7.2, respectively. In general, the optimal temperature for the fermentative hydrogen production was around 37.8°C and the optimal initial pH for the fermentative hydrogen production was around 7.1. [Copyright &y& Elsevier]

Abstract: The kinetic models were developed and applied for fermentative hydrogen production. They were used to describe the progress of a batch fermentative hydrogen production process, to investigate the effects of substrate concentration, inhibitor concentration, temperatures, pH, and dilution rates on the process of fermentative hydrogen production, and to establish the relationship among the substrate degradation rate, the hydrogen-producing bacteria growth rate and the product formation rate. This review showed that the modified Gompertz model was widely used to describe the progress of a batch fermentative hydrogen production process, while the Monod model was widely used to describe the effects of substrate concentration on the rates of substrate degradation, hydrogen-producing bacteria growth and hydrogen production. Arrhenius model was used a lot to describe the effects of temperature on fermentative hydrogen production, while modified Han–Levenspiel model was used to describe the effects of inhibitor concentration on fermentative hydrogen production. The Andrew model was used to describe the effects of H+ concentration on the specific hydrogen production rate, while the Luedeking–Piret model and its modified form were widely used to describe the relationship between the hydrogen-producing bacteria growth rate and the product formation rate. Finally, some suggestions for future work with these kinetic models were proposed. [Copyright &y& Elsevier]

Abstract: A fractional factorial design was carried out to investigate the effects of temperature, initial pH and glucose concentration on fermentative hydrogen production by mixed cultures in batch tests and then the experimental data of substrate degradation efficiency, hydrogen yield and average hydrogen production rate were described by a neural network, based on which the simultaneous optimization of the three responses was performed by the method of desirability function. The analysis showed that the neural network could successfully describe the effects of temperature, initial pH and glucose concentration on the substrate degradation efficiency, hydrogen yield and average hydrogen production rate of this study. The maximum substrate degradation efficiency of 95.3%, hydrogen yield of 305.3mL/g glucose and average hydrogen production rate of 23.9mL/h were all obtained at the optimal temperature of 39.0°C, initial pH of 7.0 and glucose concentration of 24.6g/L identified by the method of desirability function based on a neural network. In sum, the method of desirability function based on a neural network was a useful tool to optimize several responses for fermentative hydrogen production processes simultaneously. [Copyright &y& Elsevier]

Abstract: This review summarized several main factors influencing fermentative hydrogen production. The reviewed factors included inoculum, substrate, reactor type, nitrogen, phosphate, metal ion, temperature and pH. In this review, the effect of each factor on fermentative hydrogen production and the advance in the research of the effect were briefly introduced and discussed, followed by some suggestions for the future work of fermentative hydrogen production. This review showed that there usually existed some disagreements on the optimal condition of a given factor for fermentative hydrogen production, thus more researches in this respect are recommended. Furthermore, most of the studies on fermentative hydrogen production were conducted in batch mode using glucose and sucrose as substrate, thus more studies on fermentative hydrogen production in continuous mode using organic wastes as substrate are recommended. [Copyright &y& Elsevier]

Abstract: This review summarized the experimental design methods used to investigate the effects of various factors on fermentative hydrogen production processes, including one-factor-at-a-time design, full factorial design, Taguchi design, Plackett–Burman design, central composite design and Box–Behnken design. Each design method was briefly introduced, followed by the introduction of its analysis. In addition, the advantages and disadvantages of each design method were briefly discussed. Moreover, the application of each design method to the study of fermentative hydrogen production was analyzed and discussed. Based on the discussion in this review, an experimental design strategy for optimizing fermentative hydrogen production processes was proposed. In the end, the software packages that can carry out the above mentioned factorial design and analysis were briefly introduced. [Copyright &y& Elsevier]

Abstract: The inhibitory effect of added ethanol, acetic acid, propionic acid and butyric acid on fermentative hydrogen production by mixed cultures was investigated in batch tests using glucose as substrate. The experimental results showed that, at 35°C and initial pH 7.0, during the fermentative hydrogen production, the substrate degradation efficiency, hydrogen production potential, hydrogen yield and hydrogen production rate all trended to decrease with increasing added ethanol, acetic acid, propionic acid and butyric acid concentration from 0 to 300mmol/L. The inhibitory effect of added ethanol on fermentative hydrogen production was smaller than those of added acetic acid, propionic acid and butyric acid. The modified Han–Levenspiel model could describe the inhibitory effects of added ethanol, acetic acid, propionic acid and butyric acid on fermentative hydrogen production rate in this study successfully. The modified Logistic model could describe the progress of cumulative hydrogen production. [Copyright &y& Elsevier]

Abstract: The effect of temperature, initial pH and glucose concentration on fermentative hydrogen production by mixed cultures was investigated in batch tests, and the optimization of fermentative hydrogen production process was conducted by response surface methodology with a central composite design. Experimental results showed that temperatures, initial pH and glucose concentrations had impact on fermentative hydrogen production individually and interactively. The maximum hydrogen yield of 289.8mL/g glucose was estimated at the temperature of 38.6°C, the initial pH of 7.2 and the glucose concentration of 23.9g/L. The maximum hydrogen production rate of 28.2mL/h was estimated at the temperature of 37.8°C, the initial pH of 7.2 and the glucose concentration of 27.6g/L. The maximum substrate degradation efficiency of 96.9% was estimated at the temperature of 39.3°C, the initial pH of 7.0 and the glucose concentration of 26.8g/L. Response surface methodology was a better method to optimize the fermentative hydrogen production process. Modified logistic model could describe the progress of cumulative hydrogen production in the batch tests of this study successfully. [Copyright &y& Elsevier]

The effect of ammonia concentration ranging from 0 to 10g N/L on fermentative hydrogen production by mixed cultures was investigated in batch tests using glucose as substrate at 35°C and initial pH 7.0. The experimental results showed that during the fermentative hydrogen production, the substrate degradation efficiency increased with increasing ammonia concentration from 0 to 0.01g N/L. The hydrogen production potential, hydrogen yield and average hydrogen production rate increased with increasing ammonia concentration from 0 to 0.1g N/L. The maximum hydrogen production potential of 291.4mL, maximum hydrogen yield of 298.8mL/g glucose and maximum average hydrogen production rate of 8.5mL/h were all obtained at the ammonia concentration of 0.1g N/L. [Copyright &y& Elsevier]

Abstract: The effects of medium composition and fermentation parameters on simultaneous production of mycelia and intracellular polysaccharide by medicinal mushroom, Cordyceps jiangxiensis were investigated in shake-flask cultures using desirability functions. The optimal conditions were obtained via the associated desirability as follows: 40g/l of molasses glycerol, 5g/l of peanut steep meal, 6g/l of yeast extract, 1g/l of KH2PO4, 0.5g/l of ZnCl2, 0.5g/l of MgSO4, initial pH at 6, cultivation temperature at 28°C, 200ml of medium working volume in a 500ml flask and 5% (v/v) of inoculum size. Under these conditions, the production titer of mycelia and intracellular polysaccharide reached 24.5 and 8.9g/l, respectively, after a 9-day submerged cultivation. The use of desirability functions was proved effective in optimizing multi-response fermentation processes and it is readily applicable to other fermentation systems. [Copyright &y& Elsevier]