1. Suberoylanilide hydroxamic acid enhances odontoblast differentiation.
- Author
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Kwon A, Park HJ, Baek K, Lee HL, Park JC, Woo KM, Ryoo HM, and Baek JH
- Subjects
- Animals, Cell Line, Extracellular Matrix Proteins genetics, Gene Knockdown Techniques, Histone Deacetylases metabolism, Mice, NFI Transcription Factors genetics, NFI Transcription Factors metabolism, Phosphoproteins genetics, Promoter Regions, Genetic, RNA, Small Interfering physiology, Sialoglycoproteins genetics, Up-Regulation, Vorinostat, Cell Differentiation drug effects, Extracellular Matrix Proteins biosynthesis, Histone Deacetylase Inhibitors pharmacology, Hydroxamic Acids pharmacology, Odontoblasts drug effects, Phosphoproteins biosynthesis, Sialoglycoproteins biosynthesis
- Abstract
Previous studies have shown that histone deacetylase (HDAC) inhibitors stimulate osteoblast differentiation in vitro and bone formation in vivo. However, the effects of HDAC inhibitors on odontoblasts have not been elucidated. Therefore, in this study, we examined the effect of suberoylanilide hydroxamic acid (SAHA), an HDAC inhibitor, on odontoblast differentiation using an MDPC23 odontoblast-like cell line. SAHA significantly enhanced matrix mineralization and the expression levels of odontoblast marker genes. SAHA increased the expression levels of nuclear factor I/C (Nfic) and dentin sialophosphoprotein (Dspp). Nfic bound directly to the Dspp promoter and stimulated Dspp transcription. SAHA increased both basal and Nfic-induced Dspp promoter activity. SAHA-induced Dspp promoter activity disappeared when mutations were introduced within the Nfic binding element of the Dspp promoter. Nfic knockdown by siRNA blocked SAHA stimulation of Dspp expression. These results indicate that SAHA enhances odontoblast differentiation and that SAHA increases Dspp expression, at least in part, by increasing the expression level of Nfic.
- Published
- 2012
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