Search

Copyright of Food & Machinery is the property of Food & Machinery Editorial Office and its content may not be copied or emailed to multiple sites or posted to a listserv without the copyright holder's express written permission. However, users may print, download, or email articles for individual use. This abstract may be abridged. No warranty is given about the accuracy of the copy. Users should refer to the original published version of the material for the full abstract. (Copyright applies to all Abstracts.)

Flavonoid-C-glycosides, as one kind of the effective components in Premna fulva, have the effect of antigen-induced arthritis. In order to optimize the enrichment and purification process of P. fulva stems, through static adsorption and desorption experiments on 13 kinds of macroporous resins with five flavonoid-C-glycosides in P. fulva stems as the index, a suitable macroporous resin was selected, and the results were tested by the HPLC, and then the orthogonal process was used to optimize the enrichment and purification conditions. The results were as follows:(1)XAD-16N macroporous resin has good adsorption and desorption effects on the five flavonoid -C-glycosides in P. fulva stems. Through single factor screening and orthogonal process optimization, the best enrichment and purification process was finally determined as that the loading concentration was two times the original extract diluted, the loading flow rate was 2 BV·h-1, and the eluent concentration was 60% ethanol.(2)The contents of five flavonoid-C-glycosides increased from 1.73%(in the original plant)to 14.50%, an increase of 7.38 times. All the results indicate that the selected extraction process is stable and feasible, and the selected macroporous resin has good separation and purification effects, which provides a reference for the drug development of P. fulva stems. [ABSTRACT FROM AUTHOR]