1. Characterization of the proteome of cytoplasmic lipid droplets in mouse enterocytes after a dietary fat challenge.
- Author
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D'Aquila T, Sirohi D, Grabowski JM, Hedrick VE, Paul LN, Greenberg AS, Kuhn RJ, and Buhman KK
- Subjects
- Animals, Apolipoproteins A metabolism, Carrier Proteins metabolism, Coenzyme A Ligases metabolism, Enterocytes ultrastructure, Lipid Droplets ultrastructure, Lipid Metabolism, Male, Metabolic Networks and Pathways, Mice, Mice, Inbred C57BL, Microscopy, Electron, Transmission, Models, Biological, Perilipin-3, Triglycerides metabolism, Dietary Fats administration & dosage, Enterocytes metabolism, Lipid Droplets metabolism, Proteome metabolism
- Abstract
Dietary fat absorption by the small intestine is a multistep process that regulates the uptake and delivery of essential nutrients and energy. One step of this process is the temporary storage of dietary fat in cytoplasmic lipid droplets (CLDs). The storage and mobilization of dietary fat is thought to be regulated by proteins that associate with the CLD; however, mechanistic details of this process are currently unknown. In this study we analyzed the proteome of CLDs isolated from enterocytes harvested from the small intestine of mice following a dietary fat challenge. In this analysis we identified 181 proteins associated with the CLD fraction, of which 37 are associated with known lipid related metabolic pathways. We confirmed the localization of several of these proteins on or around the CLD through confocal and electron microscopy, including perilipin 3, apolipoprotein A-IV, and acyl-CoA synthetase long-chain family member 5. The identification of the enterocyte CLD proteome provides new insight into potential regulators of CLD metabolism and the process of dietary fat absorption.
- Published
- 2015
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