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Characterization of the proteome of cytoplasmic lipid droplets in mouse enterocytes after a dietary fat challenge.
- Source :
-
PloS one [PLoS One] 2015 May 18; Vol. 10 (5), pp. e0126823. Date of Electronic Publication: 2015 May 18 (Print Publication: 2015). - Publication Year :
- 2015
-
Abstract
- Dietary fat absorption by the small intestine is a multistep process that regulates the uptake and delivery of essential nutrients and energy. One step of this process is the temporary storage of dietary fat in cytoplasmic lipid droplets (CLDs). The storage and mobilization of dietary fat is thought to be regulated by proteins that associate with the CLD; however, mechanistic details of this process are currently unknown. In this study we analyzed the proteome of CLDs isolated from enterocytes harvested from the small intestine of mice following a dietary fat challenge. In this analysis we identified 181 proteins associated with the CLD fraction, of which 37 are associated with known lipid related metabolic pathways. We confirmed the localization of several of these proteins on or around the CLD through confocal and electron microscopy, including perilipin 3, apolipoprotein A-IV, and acyl-CoA synthetase long-chain family member 5. The identification of the enterocyte CLD proteome provides new insight into potential regulators of CLD metabolism and the process of dietary fat absorption.
- Subjects :
- Animals
Apolipoproteins A metabolism
Carrier Proteins metabolism
Coenzyme A Ligases metabolism
Enterocytes ultrastructure
Lipid Droplets ultrastructure
Lipid Metabolism
Male
Metabolic Networks and Pathways
Mice
Mice, Inbred C57BL
Microscopy, Electron, Transmission
Models, Biological
Perilipin-3
Triglycerides metabolism
Dietary Fats administration & dosage
Enterocytes metabolism
Lipid Droplets metabolism
Proteome metabolism
Subjects
Details
- Language :
- English
- ISSN :
- 1932-6203
- Volume :
- 10
- Issue :
- 5
- Database :
- MEDLINE
- Journal :
- PloS one
- Publication Type :
- Academic Journal
- Accession number :
- 25992653
- Full Text :
- https://doi.org/10.1371/journal.pone.0126823