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Your search keyword '"Promoters (Genetics) -- Structure"' showing total 2 results
Start Over Descriptor "Promoters (Genetics) -- Structure" Topic energy transformation -- analysis
2 results on '"Promoters (Genetics) -- Structure"'

1. T7 RNA polymerase-induced bending of promoter DNA is coupled to DNA opening

Author

Guo-Qing Tang and Patel, Smita S.

Subjects
RNA polymerases -- Structure, RNA polymerases -- Chemical properties, Promoters (Genetics) -- Structure, Promoters (Genetics) -- Chemical properties, Energy transformation -- Analysis, Biological sciences, Chemistry
Abstract

Fluorescence resonance energy transfer (FRET) is used to investigate the conformation of promoter DNA in the closed and open complexes of T7 RNA polymerase (T7 RNA P). It is proposed that promoter opening occurs spontaneously upon DNA bending and/or untwisting as free energy is gained through interactions of the melted promoter with the T7 RNAP active site.

Published
2006

2. Rapid binding of T7 RNA polymerase is followed by simultaneous bending and opening of the promoter DNA

Author

Guo-Qing Tang and Patel, Smita S.

Subjects
RNA polymerases -- Chemical properties, RNA polymerases -- Structure, DNA-ligand interactions -- Analysis, Promoters (Genetics) -- Structure, Promoters (Genetics) -- Chemical properties, Energy transformation -- Analysis, Biological sciences, Chemistry
Abstract

Elucidation of the temporal coupling in DNA binding, bending, and opening processes that occur during initiation is presented. The results provided are consistent with the mechanism in which the initial binding of T7 RNAP to the promoter results in a closed complex, which is then converted into an open complex in which promoter is both sharply bent and melted.

Published
2006

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