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T7 RNA polymerase-induced bending of promoter DNA is coupled to DNA opening

Authors :
Guo-Qing Tang
Patel, Smita S.
Source :
Biochemistry. April 18, 2006, Vol. 45 Issue 15, p4936, 11 p.
Publication Year :
2006

Abstract

Fluorescence resonance energy transfer (FRET) is used to investigate the conformation of promoter DNA in the closed and open complexes of T7 RNA polymerase (T7 RNA P). It is proposed that promoter opening occurs spontaneously upon DNA bending and/or untwisting as free energy is gained through interactions of the melted promoter with the T7 RNAP active site.

Subjects

Subjects :
RNA polymerases -- Structure
RNA polymerases -- Chemical properties
Promoters (Genetics) -- Structure
Promoters (Genetics) -- Chemical properties
Energy transformation -- Analysis
Biological sciences
Chemistry

Details

Language :
English
ISSN :
00062960
Volume :
45
Issue :
15
Database :
Gale General OneFile
Journal :
Biochemistry
Publication Type :
Academic Journal
Accession number :
edsgcl.146731821

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