1. Melatonin inhibits 17β-estradiol-induced migration, invasion and epithelial-mesenchymal transition in normal and endometriotic endometrial epithelial cells.
- Author
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Qi S, Yan L, Liu Z, Mu YL, Li M, Zhao X, Chen ZJ, and Zhang H
- Subjects
- Antioxidants pharmacology, Cell Movement drug effects, Cell Movement physiology, Cells, Cultured, Endometriosis pathology, Endometrium drug effects, Epithelial Cells drug effects, Epithelial-Mesenchymal Transition drug effects, Female, Humans, Receptor, Notch1 metabolism, Endometriosis metabolism, Endometrium metabolism, Epithelial Cells metabolism, Epithelial-Mesenchymal Transition physiology, Estradiol pharmacology, Melatonin pharmacology
- Abstract
Background: Melatonin is a potential therapeutic agent for endometriosis, but its molecular mechanism is unclear. Here, we investigated the effect of melatonin on the epithelial-mesenchymal transition (EMT) in endometriotic endometrial epithelial cells and explored the pathway that might be involved., Methods: This hospital-based study included 60 women of reproductive age using the endometrium for immunohistochemistry, 6 women of reproductive age undergoing bilateral tubal ligation and 6 patients with endometriosis for isolation of endometrial epithelial cells or subsequent analysis, respectively. We examined the expression of Notch1/Numb signaling and EMT markers by immunohistochemistry analysis and western blot analysis, the invasion and migration of endometrial epithelial cells by transwell assays, and the cell proliferation by CCK8 assays., Results: Compared with normal endometrium, the endometriotic eutopic endometrium showed increased expression of Notch1, Slug, Snail, and N-cadherin, and decreased expression of E-cadherin and Numb. Melatonin or Notch inhibition by specific inhibitor blocked 17β-estradiol-induced cell proliferation, invasion, migration and EMT-related markers in both normal and endometriotic epithelial cells., Conclusions: Our data suggest that aberrant expression of Notch1/Numb signaling and the EMT is present in endometriotic endometrium. Melatonin may block 17β-estradiol-induced migration, invasion and EMT in normal and endometriotic epithelial cells by upregulating Numb expression and decreasing the activity of the Notch signaling pathway.
- Published
- 2018
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