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Melatonin inhibits 17β-estradiol-induced migration, invasion and epithelial-mesenchymal transition in normal and endometriotic endometrial epithelial cells.
- Source :
-
Reproductive biology and endocrinology : RB&E [Reprod Biol Endocrinol] 2018 Jun 23; Vol. 16 (1), pp. 62. Date of Electronic Publication: 2018 Jun 23. - Publication Year :
- 2018
-
Abstract
- Background: Melatonin is a potential therapeutic agent for endometriosis, but its molecular mechanism is unclear. Here, we investigated the effect of melatonin on the epithelial-mesenchymal transition (EMT) in endometriotic endometrial epithelial cells and explored the pathway that might be involved.<br />Methods: This hospital-based study included 60 women of reproductive age using the endometrium for immunohistochemistry, 6 women of reproductive age undergoing bilateral tubal ligation and 6 patients with endometriosis for isolation of endometrial epithelial cells or subsequent analysis, respectively. We examined the expression of Notch1/Numb signaling and EMT markers by immunohistochemistry analysis and western blot analysis, the invasion and migration of endometrial epithelial cells by transwell assays, and the cell proliferation by CCK8 assays.<br />Results: Compared with normal endometrium, the endometriotic eutopic endometrium showed increased expression of Notch1, Slug, Snail, and N-cadherin, and decreased expression of E-cadherin and Numb. Melatonin or Notch inhibition by specific inhibitor blocked 17β-estradiol-induced cell proliferation, invasion, migration and EMT-related markers in both normal and endometriotic epithelial cells.<br />Conclusions: Our data suggest that aberrant expression of Notch1/Numb signaling and the EMT is present in endometriotic endometrium. Melatonin may block 17β-estradiol-induced migration, invasion and EMT in normal and endometriotic epithelial cells by upregulating Numb expression and decreasing the activity of the Notch signaling pathway.
- Subjects :
- Antioxidants pharmacology
Cell Movement drug effects
Cell Movement physiology
Cells, Cultured
Endometriosis pathology
Endometrium drug effects
Epithelial Cells drug effects
Epithelial-Mesenchymal Transition drug effects
Female
Humans
Receptor, Notch1 metabolism
Endometriosis metabolism
Endometrium metabolism
Epithelial Cells metabolism
Epithelial-Mesenchymal Transition physiology
Estradiol pharmacology
Melatonin pharmacology
Subjects
Details
- Language :
- English
- ISSN :
- 1477-7827
- Volume :
- 16
- Issue :
- 1
- Database :
- MEDLINE
- Journal :
- Reproductive biology and endocrinology : RB&E
- Publication Type :
- Academic Journal
- Accession number :
- 29935526
- Full Text :
- https://doi.org/10.1186/s12958-018-0375-5