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1. Hyaluronic Acid/Ellagic Acid as Materials for Potential Medical Application.

Author

Kaczmarek-Szczepańska B, Kleszczyński K, Zasada L, Chmielniak D, Hollerung MB, Dembińska K, Pałubicka K, Steinbrink K, Swiontek Brzezinska M, and Grabska-Zielińska S

Subjects
Humans, Pseudomonas aeruginosa drug effects, Anti-Bacterial Agents pharmacology, Anti-Bacterial Agents chemistry, Antioxidants pharmacology, Antioxidants chemistry, Fibroblasts drug effects, Keratinocytes drug effects, Microbial Sensitivity Tests, Cell Line, Tumor, Surface Properties, Hyaluronic Acid chemistry, Hyaluronic Acid pharmacology, Staphylococcus aureus drug effects, Biocompatible Materials chemistry, Biocompatible Materials pharmacology, Ellagic Acid pharmacology, Ellagic Acid chemistry
Abstract

The aim of this work was to develop and characterize a thin films composed of hyaluronic acid/ellagic acid for potential medical application. Its principal novelty, distinct from the prior literature in terms of hyaluronic acid films supplemented with phenolic acids, resides in the predominant incorporation of ellagic acid-a distinguished compound-as the primary constituent of the films. Herein, ellagic acid was dissolved in two different solvents, i.e., acetic acid (AcOH) or sodium hydroxide (NaOH), and the surface properties of the resultant films were assessed using atomic force microscopy and contact angle measurements. Additionally, various physicochemical parameters were evaluated including moisture content, antioxidant activity, and release of ellagic acid in phosphate buffered saline. Furthermore, the evaluation of films' biocompatibility was conducted using human epidermal keratinocytes, dermal fibroblasts, and human amelanotic melanoma cells (A375 and G361), and the antimicrobial activity was elucidated accordingly against Staphylococcus aureus ATCC 6538 and Pseudomonas aeruginosa ATCC 15442. Our results showed that the films exhibited prominent antibacterial properties particularly against Staphylococcus aureus , with the 80HA/20EA/AcOH film indicating the strong biocidal activity against this strain leading to a significant reduction in viable cells. Comparatively, the 50HA/50EA/AcOH film also displayed biocidal activity against Staphylococcus aureus . This experimental approach could be a promising technique for future applications in regenerative dermatology or novel strategies in terms of bioengineering.

Published
2024
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2. Comparison of the antiinflammatory effects ofDrosera rotundifolia andDrosera madagascariensis in the HET-CAM assay

Author

Liselotte Krenn, Michaela Kremser, Elisabeth Karall, and D. H. Paper

Subjects
Drosera madagascariensis, Drosera, Flavonoid, Chick Embryo, Pharmacognosy, law.invention, chemistry.chemical_compound, Allantois, law, Botany, Animals, Humans, Inflammation, Pharmacology, chemistry.chemical_classification, Dose-Response Relationship, Drug, biology, Traditional medicine, Plant Extracts, Anti-Inflammatory Agents, Non-Steroidal, Plant Components, Aerial, biology.organism_classification, Drosera rotundifolia, chemistry, Polyphenol, Biological Assay, Phytotherapy, Ellagic acid
Abstract

The antiinflammatory effects of ethanol and aqueous extracts from Drosera rotundifolia and from Drosera madagascariensis were compared in vivo in the HET-CAM assay. Both extracts from D. rotundifolia and the ethanol extract from D. madagascariensis showed remarkable efficacy at doses of 500 microg/pellet. The inhibition of the inflammation by the extracts was stronger than that by 50 microg hydrocortisone/pellet. In contrast, there was only a very weak effect observed at a dose of 500 microg/pellet of the water extract from D. madagascariensis. The chemical analyses of the extracts showed that the effect cannot be attributed to naphthoquinones, but might be due to flavonoids. Ellagic acid obviously plays an important role in the antiangiogenic effect of the Drosera extracts.

Published
2005