14 results on '"Mubashar Rehman"'
Search Results
2. Facile and Novel Synthesis of Spiky Gold Nanoparticles as an Efficient Antimicrobial Agent against Pseudomonas Aeruginosa
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Farooq Aziz, Mubashar Rehman, Muhammad Rafique, Muhammad Rashid, and Muhammad Imran
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biology ,Pseudomonas aeruginosa ,Chemistry ,Organic Chemistry ,Metal Nanoparticles ,Nanoparticle ,Microbial Sensitivity Tests ,General Medicine ,Antimicrobial ,biology.organism_classification ,medicine.disease_cause ,Combinatorial chemistry ,Anti-Bacterial Agents ,Computer Science Applications ,Anti-Infective Agents ,Colloidal gold ,Drug Discovery ,medicine ,Gold ,Surface plasmon resonance ,Antibacterial activity ,Bacteria ,Antibacterial agent - Abstract
Aims: The study aims to develop advanced antibacterial agents as nanoparticles instead of antibiotics due to the emergence of antimicrobial resistance. Background: Pseudomonas aeruginosa is capable of causing many diseases, including severe bacterial pneumonia. There is a need for an efficient antibacterial agent to kill these pathogens. Objective: The objective of the study is to synthesize advanced antibacterial agents as nanoparticles for biomedical applications that can play a vital role in killing Gram-negative bacteria (Pseudomonas aeruginosa). Method: A novel fabricated growth of hydrophilic spiky gold nanoparticles (SGNPs) via reduction method is reported. Results: The surface plasmon resonance peak of the synthesized SGNPs was tuned under the near-infrared range. The SGNPs have anisotropic and spiky morphology with 68 nm size and -58 mV surface charge. They are pure, possessing adsorption similar to the organic material. Pseudomonas aeruginosa treated with synthesized SGNPs showed 60% bacterial death at the concentration of 100 μM. Conclusion: This work consists of the novel synthesis of SGNPs via a safe and simple reduction method. The synthesized SGNPs exhibit strong antibacterial activity against the Gram-negative bacteria Pseudomonas aeruginosa measured using a microplate assay test. The result showed that these SGNPs are ideal for biomedical applications.
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- 2022
3. Investigation of Eutectic Mixtures of Fatty Acids as a Novel Construct for Temperature-Responsive Drug Delivery
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Farzana Parveen, Asadullah Madni, Vladimir P Torchilin, Mubashar Rehman, Talha Jamshaid, Nina Filipczak, Nadia Rai, Muhammad Muzamil Khan, and Muhammad Imran Khan
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Fatty Acids ,Organic Chemistry ,Temperature ,Biophysics ,Pharmaceutical Science ,Bioengineering ,General Medicine ,Biomaterials ,Drug Liberation ,Microscopy, Electron, Transmission ,Doxorubicin ,International Journal of Nanomedicine ,Neoplasms ,Drug Discovery ,Humans - Abstract
Farzana Parveen,1â 3 Asadullah Madni,2 Vladimir P Torchilin,1 Mubashar Rehman,4 Talha Jamshaid,2 Nina Filipczak,1 Nadia Rai,2,5 Muhammad Muzamil Khan,2,3 Muhammad Imran Khan6 1Center for Pharmaceutical Biotechnology and Nanomedicine, Northeastern University, Boston, MA, 02115, USA; 2Department of Pharmaceutics, Faculty of Pharmacy, The Islamia University of Bahawalpur, Bahawalpur, Punjab, 63100, Pakistan; 3Primary and Secondary Healthcare Department, Government of Punjab, Lahore, 54000, Pakistan; 4Department of Pharmacy, Quaid-i-Azam University, Islamabad, 45320, Pakistan; 5Department of Biomedical and Pharmaceutical Sciences, Chapman University School of Pharmacy, Irvine, CA, 92618, USA; 6Riphah Institute of Pharmaceutical Sciences, Riphah International University Lahore Campus, Lahore, 54000, PakistanCorrespondence: Asadullah Madni, Department of Pharmaceutics, Faculty of Pharmacy, The Islamia University of Bahawalpur, Bahawalpur, Punjab, 63100, Pakistan, Tel +92 62 925 5243, Fax +92 62 925 5565, Email asadpharmacist@hotmail.comBackground: Most of the traditional nanocarriers of cancer therapeutic moieties present dose-related toxicities due to the uptake of chemotherapeutic agents in normal body cells. The severe life-threatening effects of systemic chemotherapy are well documented. Doxorubicin, DOX is the most effective antineoplastic agent but with the least specific action that is responsible for severe cardiotoxicity and myelosuppression that necessitates careful monitoring while administering. Stimuli-sensitive/intelligent drug delivery systems, specifically those utilizing temperature as an external stimulus to activate the release of encapsulated drugs, have become a subject of recent research. Thus, it would be ideal to have a nanocarrier comprising safe excipients and controllable drug release capacity to deliver the drug at a particular site to minimize unwanted and toxic effects of chemotherapeutics. We have developed a simple temperature-responsive nanocarrier based on eutectic mixture of fatty acids. This study aimed to develop, physicochemically characterize and investigate the biological safety of eutectic mixture of fatty acids as a novel construct for temperature-responsive drug release potential.Methods: We have developed phase change material, PCM, based on a series of eutectic mixtures of fatty acids due to their unique and attractive physicochemical characteristics such as safety, stability, cost-effectiveness, and ease of availability. The reversible solid-liquid phase transition of PCM is responsible to hold firm or actively release the encapsulated drug. The eutectic mixtures of fatty acids (stearic acid and myristic acid) along with liquid lipid (oleic acid) were prepared to exhibit a tunable thermoresponsive platform. Doxorubicin-loaded lipid nanocarriers were successfully developed with combined hot melt encapsulation (HME) and sonication method and characterized to achieve enhanced permeability and retention (EPR) effect-based solid tumor targeting in response to exogenous temperature stimulus. The cytotoxicity against melanoma cell lines and in vivo safety studies in albino rats was also carried out.Results: Doxorubicin-loaded lipid nanocarriers have a narrow size distribution (94.59â 219.3 nm), and a PDI (0.160â 0.479) as demonstrated by photon correlation microscopy and excellent colloidal stability (Z.P value: â 22.7 to â 32.0) was developed. Transmission electron microscopy revealed their spherical morphology and characteristics of a monodispersed system. A biphasic drug release pattern with a triggered drug release at 41°C and 43°C and a sustained drug release was observed at 37°C. The thermoresponsive cytotoxic potential was demonstrated in B16F10 cancer cell lines. Hemolysis assay and acute toxicity studies with drug-free and doxorubicin lipid nanocarrier formulations provided evidence for their non-toxic nature.Conclusion: We have successfully developed a temperature-responsive tunable platform with excellent biocompatibility and intelligent drug release potential. The formulation components being from natural sources present superior characteristics in terms of cost, compatibility with normal body cells, and adaptability to preparation methods. The reported preparation method is adapted to avoid complex chemical processes and the use of organic solvents. The lipid nanocarriers with tunable thermoresponsive characteristics are promising biocompatible drug delivery systems for improved localized delivery of chemotherapeutic agents.Graphical Abstract: Keywords: nanostructured lipid carriers, lipid nanocarriers, thermoresponsive, eutectic mixtures, fatty acids, phase change materials, doxorubicin, acute toxicity studies, hemolysis assay
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- 2022
4. Ionically Cross-Linked Chitosan Nanoparticles for Sustained Delivery of Docetaxel: Fabrication, Post-Formulation and Acute Oral Toxicity Evaluation
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Mubashar Rehman, Asadullah Madni, Muhammad Abdur Rahim, Muhammad Ahmad Mahmood, and Abdul Jabar
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Male ,biocompatible ,Biophysics ,Administration, Oral ,Pharmaceutical Science ,Nanoparticle ,Antineoplastic Agents ,Bioengineering ,Docetaxel ,02 engineering and technology ,010402 general chemistry ,tripolyphosphate ,01 natural sciences ,Biomaterials ,Chitosan ,chemistry.chemical_compound ,Drug Delivery Systems ,Polyphosphates ,International Journal of Nanomedicine ,Drug Discovery ,Toxicity Tests, Acute ,medicine ,Zeta potential ,Animals ,ionic gelation ,Rats, Wistar ,sustained release ,targeting ,Original Research ,Taxane ,Chemistry ,Organic Chemistry ,General Medicine ,021001 nanoscience & nanotechnology ,0104 chemical sciences ,Drug Liberation ,Cross-Linking Reagents ,Solubility ,Paclitaxel ,Toxicity ,Drug delivery ,Nanoparticles ,biodegradable ,Rabbits ,0210 nano-technology ,medicine.drug ,Nuclear chemistry - Abstract
Muhammad Ahmad Mahmood,1,2 Asadullah Madni,1 Mubashar Rehman,3 Muhammad Abdur Rahim,1 Abdul Jabar1 1Department of Pharmacy, The Islamia University of Bahawalpur, Bahawalpur 63100, Pakistan; 2Drug Testing Laboratory, Bahawalpur 63100, Pakistan; 3Faculty of Pharmacy, University of Central Punjab, Lahore 54000, PakistanCorrespondence: Asadullah MadniDepartment of Pharmacy, The Islamia University of Bahawalpur, Khawaja Fareed Campus, Railway Road, Bahawalpur, Punjab, PakistanTel +92 62 925 5243Fax +92 62 925 5565Email asadpharmacist@hotmail.comIntroduction: Polymeric nanoparticles are potential carriers for the efficient delivery of hydrophilic and hydrophobic drugs due to their multifaceted applications. Docetaxel is relatively less hydrophobic and twice as potent as paclitaxel. Like other taxane chemotherapeutic agents, docetaxel is not well tolerated and shows toxicity in the patients. Nanoencapsulation of potent chemotherapeutic agents has been shown to improve tolerability and therapeutic outcome. Therefore, the present study was designed to fabricate chitosan and sodium tripolyphosphate (STPP) based on ionically cross-linked nanoparticles for sustained release of docetaxel.Methods: Nanoparticles were prepared by the ionic-gelation method by dropwise addition of the STPP solution into the chitosan solution in different ratios. CNPs were characterized for post-formulation parameters like size, zeta potential, scanning electron microscope (SEM), FTIR, DSC/TGA, pXRD, and in-vitro drug release, as well as for acute oral toxicity studies in Wistar rats.Results and discussion: The optimized docetaxel loaded polymeric nanoparticles were in the size range (172.6nm–479.65 nm), and zeta potential (30.45–35.95 mV) required to achieve enhanced permeation and retention effect. In addition, scanning electron microscopy revealed rough and porous surface, whereas, FTIR revealed the compatible polymeric nanoparticles. Likewise, the thermal stability was ensured through DSC and TG analysis, and powder X-ray diffraction analysis exhibited solid-state stability of the docetaxel loaded nanoparticles. The in-vitro drug release evaluation in phosphate buffer saline (pH 7.4) showed sustained release pattern, i.e. 51.57–69.93% within 24 hrs. The data were fitted to different release kinetic models which showed Fickian diffusion as a predominant release mechanism (R2= 0.9734–0.9786, n= 0.264–0.340). Acceptable tolerability was exhibited by acute oral toxicity in rabbits and no abnormality was noted in growth, behavior, blood biochemistry or histology and function of vital organs.Conclusion: Ionically cross-linked chitosan nanoparticles are non-toxic and biocompatible drug delivery systems for sustained release of chemotherapeutic agents, such as docetaxel.Keywords: tripolyphosphate, ionic gelation, sustained release, targeting, biocompatible, biodegradable
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- 2019
5. Lipid-polymer hybrid nanoparticles for controlled delivery of hydrophilic and lipophilic doxorubicin for breast cancer therapy
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Nayab Tahir, Mubashar Rehman, Hélder A. Santos, Muhammad Muzamil Khan, Vimalkumar Balasubramanian, Asadullah Madni, and Alexandra Maria Rebelo Correia
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food.ingredient ,Biocompatibility ,Biophysics ,Pharmaceutical Science ,Bioengineering ,macromolecular substances ,02 engineering and technology ,Polyethylene glycol ,010402 general chemistry ,01 natural sciences ,Lecithin ,Biomaterials ,chemistry.chemical_compound ,food ,Drug Discovery ,polycyclic compounds ,medicine ,Doxorubicin ,Chemistry ,Organic Chemistry ,technology, industry, and agriculture ,General Medicine ,021001 nanoscience & nanotechnology ,Controlled release ,3. Good health ,0104 chemical sciences ,carbohydrates (lipids) ,PLGA ,Drug delivery ,Doxorubicin Hydrochloride ,0210 nano-technology ,Nuclear chemistry ,medicine.drug - Abstract
Background: Lipid polymer hybrid nanoparticles (LPHNPs) for the controlled delivery of hydrophilic doxorubicin hydrochloride (DOX.HCl) and lipophilic DOX base have been fabricated by the single step modified nanoprecipitation method. Materials and methods: Poly (D, L-lactide-co-glicolide) (PLGA), lecithin, and 1,2-distearoyl-Sn-glycero-3-phosphoethanolamine-N-[methoxy (polyethylene glycol)-2000 (DSPE-PEG 2000) were selected as structural components. Results: The mean particle size was 173-208 nm, with an encapsulation efficiency of 17.8±1.9 to 43.8±4.4% and 40.3±0.6 to 59. 8±1.4% for DOX.HCl and DOX base, respectively. The drug release profile was in the range 33-57% in 24 hours and followed the Higuchi model (R2=0.9867-0.9450) and Fickian diffusion (n
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- 2019
6. Advancements in the oral delivery of Docetaxel: challenges, current state-of-the-art and future trends
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Sara Naveed, Mubashar Rehman, Thomas J. Webster, Nadeem Irfan Bukhari, Muhammad Farhan Sohail, Gul Shahnaz, Irshad Hussain, Omer Salman, and Hafiz Shoaib Sarwar
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Dendrimers ,medicine.medical_specialty ,Polymers ,Biophysics ,Administration, Oral ,Biological Availability ,Pharmaceutical Science ,Antineoplastic Agents ,Bioengineering ,Docetaxel ,02 engineering and technology ,Biomaterials ,03 medical and health sciences ,Drug Delivery Systems ,0302 clinical medicine ,Drug Discovery ,medicine ,Animals ,Humans ,Pharmaceutical sciences ,Intensive care medicine ,Drug Carriers ,business.industry ,Organic Chemistry ,General Medicine ,021001 nanoscience & nanotechnology ,Review article ,Bioavailability ,Clinical trial ,Nanomedicine ,030220 oncology & carcinogenesis ,Liposomes ,Drug delivery ,Nanoparticles ,Taxoids ,Nanocarriers ,0210 nano-technology ,business ,medicine.drug - Abstract
The oral delivery of cancer chemotherapeutic drugs is challenging due to low bioavailability, gastrointestinal side effects, first-pass metabolism and P-glycoprotein efflux pumps. Thus, chemotherapeutic drugs, including Docetaxel, are administered via an intravenous route, which poses many disadvantages of its own. Recent advances in pharmaceutical research have focused on designing new and efficient drug delivery systems for site-specific targeting, thus leading to improved bioavailability and pharmacokinetics. A decent number of studies have been reported for the safe and effective oral delivery of Docetaxel. These nanocarriers, including liposomes, polymeric nanoparticles, metallic nanoparticles, hybrid nanoparticles, dendrimers and so on, have shown promising results in research papers and clinical trials. The present article comprehensively reviews the research efforts made so far in designing various advancements in the oral delivery of Docetaxel. Different strategies to improve oral bioavailability, prevent first-pass metabolism and inhibition of efflux pumping leading to improved pharmacokinetics and anticancer activity are discussed. The final portion of this review article presents key issues such as safety of nanomaterials, regulatory approval and future trends in nanomedicine research.
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- 2018
7. Enhancement of Dissolution and Skin Permeability of Pentazocine by Proniosomes and Niosomal Gel
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Asadullah Madni, Aamna Shah, Abdul Jabar, Muhammad Ahmad Mahmood, Muhammad Abdur Rahim, Mubashar Rehman, Arshad Khan, Nayab Tahir, and Hassan Shah
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Pentazocine ,Skin Absorption ,Biological Availability ,Pharmaceutical Science ,02 engineering and technology ,Aquatic Science ,Administration, Cutaneous ,030226 pharmacology & pharmacy ,Permeability ,03 medical and health sciences ,First pass effect ,0302 clinical medicine ,X-Ray Diffraction ,Drug Discovery ,medicine ,Zeta potential ,Animals ,Prodrugs ,Niosome ,Solubility ,Dissolution ,Ecology, Evolution, Behavior and Systematics ,Skin ,Chromatography ,Ecology ,Chemistry ,General Medicine ,Permeation ,021001 nanoscience & nanotechnology ,Bioavailability ,Analgesics, Opioid ,Drug Liberation ,Liposomes ,Rabbits ,0210 nano-technology ,Gels ,Agronomy and Crop Science ,medicine.drug - Abstract
Proniosomes (PN) are the dry water-soluble carrier systems that may enhance the oral bioavailability, stability, and topical permeability of therapeutic agents. The low solubility and low oral bioavailability due to extensive first pass metabolism make Pentazocine as an ideal candidate for oral and topical sustained release delivery. The present study was aimed to formulate the PNs by quick slurry method that are converted to niosomes (liquid dispersion) by hydration, and subsequently formulated to semisolid niosomal gel. The PNs were found in spherical shape in the SEM and stable in the physicochemical and thermal analysis (FTIR, TGA, and XRD). The quick slurry method produced high recovery (> 80% yield) and better flow properties (θ = 28.1–37.4°). After hydration, the niosomes exhibited desirable entrapment efficiency (44.45–76.23%), size (4.98–21.3 μm), and zeta potential (− 9.81 to − 21.53 mV). The in vitro drug release (T100%) was extended to more than three half-lives (2–4 h) and showed good fit to Fickian diffusion indicated by Korsmeyer-Peppas model (n = 0.136–0.365 and R2 = 0.9747–0.9954). The permeation of niosomal gel was significantly enhanced across rabbit skin compared to the pure drug-derived gel. Therefore, the PNs are found promising candidates for oral as dissolution enhancement and sustained release for oral and topical delivery of pentazocine for the management of cancer pain.
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- 2018
8. Role of Computer Aided Drug Design in Modern Drug Discovery and Pharmacokinetic Prediction
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Arif Paiman, Ahmad Mohammad, and Mubashar Rehman
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Drug ,ComputingMethodologies_PATTERNRECOGNITION ,Pharmacokinetics ,Computer science ,Drug discovery ,media_common.quotation_subject ,Computer-aided ,Computational biology ,media_common - Abstract
In modern day, Data on different diseases and drug substances with their properties like modification, side effects, and dose requires documentation data and building library exploring, such library with vast information in every aspect needs computational methods used in CADD. Recognition of specific targets for the drug tested and defining pharmacological activity of a drug candidate based on the structure of both drug and its target, finding outside effects of drugs at the molecular level and calculation of toxicity caused by metabolism of drug applications of Computer aided drug design in the drug discovery process. We can get additional tools and websites which serve As a tool for the source of data and computational drug design are available on the web interface and being used extensively by researchers and scientists to save time and budget for speeding up the process of experiments for Novel Drug compound.
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- 2017
9. Strategies and Techniques of Drug Discovery from Natural Products
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Mubashar Rehman, Parniya Akbar Ali, Hosna Nettour, and Farah Hanif
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Drug discovery ,Computer science ,Computational biology ,Natural (archaeology) - Abstract
New drugs are mostly obtained from Natural sources. The traditional and ethic medicines have provided evidence on the therapeutic properties and resulted in some distinguished drug discovery of natural products. The microorganisms and the endogenous active materials from human or animal have also become a significant approach to the discovery of a drug. Bioinformatics and artificial intelligence have facilitated the study and development of products. For discovery of natural products different software have been used. Different computational software needed in the future for the predicting features in new drug development, for instance pharmacokinetic and pharmacodynamics, in drug development lead positive impact. This review focus on natural product drug discovery and uses innovative strategies and techniques as a part of discovery of drugs from natural products.
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- 2017
10. Solid Lipid Nanoparticles for Thermoresponsive Targeting: Evidence from Spectrophotometry, Electrochemical, and Cytotoxicity Studies [Corrigendum]
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Mubashar Rehman, Ayesha Ihsan, Asadullah Madni, Sadia Zafar Bajwa, Di Shi, Thomas J Webster, and Waheed Khan
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Biomaterials ,International Journal of Nanomedicine ,Organic Chemistry ,Drug Discovery ,Biophysics ,Pharmaceutical Science ,Bioengineering ,General Medicine ,Corrigendum - Abstract
Rehman M, Ihsan A, Madni A, et al. Int J Nanomedicine. 2017;12:8325–8336. The authors have advised Figure 3 on page 8332 is incorrect. An error was made in the labelling of the images with some FLOM samples incorrectly labelled as FLOD samples. The authors inadvertently selected a mislabelled FLOM sample to represent the FLOD sample without realising both the FLOM and FLOD images were the same. The correct Figure 3 is shown below. Figure 3 TEM images of (A) FLOM, (B) FLOD, (C) FLLM and (D) FLLD. Abbreviations: TEM, transmission electron microscopy; FLOM, 5-FU-loaded lauric acid and oleic acid nanoparticles prepared with mono-surfactant system; FLOD, 5-FU-loaded lauric acid and oleic acid nanoparticles prepared with double-surfactant system; FLLM, 5-FU-loaded lauric acid and linoleic acid nanoparticles prepared with mono-surfactant system; FLLD, 5-FU-loaded lauric acid and linoleic acid nanoparticles prepared with double-surfactant system; 5-FU, 5-fluorouracil. The authors apologize for this error and advise that this does not affect the results of the paper. Read the original article
- Published
- 2021
11. Lipid-Polymer Hybrid Nanoparticles for Controlled Delivery of Hydrophilic and Lipophilic Doxorubicin for Breast Cancer Therapy [Erratum]
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Nayab Tahir, Asadullah Madni, Alexandra Correia, Mubashar Rehman, Vimalkumar Balasubramanian, Muhammad Muzamil Khan, and Hélder A Santos
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Biomaterials ,Organic Chemistry ,Drug Discovery ,Biophysics ,Pharmaceutical Science ,Bioengineering ,General Medicine - Published
- 2020
12. Solid lipid nanoparticles for thermoresponsive targeting: evidence from spectrophotometry, electrochemical, and cytotoxicity studies
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Sadia Zafar Bajwa, Mubashar Rehman, Waheed S. Khan, Asadullah Madni, Ayesha Ihsan, Di Shi, and Thomas J. Webster
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Drug ,Materials science ,media_common.quotation_subject ,nanostructured lipid carriers ,Biophysics ,Pharmaceutical Science ,Bioengineering ,02 engineering and technology ,emulsions ,fatty acids ,Biomaterials ,03 medical and health sciences ,chemistry.chemical_compound ,0302 clinical medicine ,Differential scanning calorimetry ,breast cancer ,International Journal of Nanomedicine ,Drug Discovery ,Solid lipid nanoparticle ,Organic chemistry ,5-fluorouracil ,Viability assay ,Cytotoxicity ,media_common ,Original Research ,Chromatography ,Organic Chemistry ,General Medicine ,021001 nanoscience & nanotechnology ,temperature sensitive ,body regions ,Oleic acid ,chemistry ,030220 oncology & carcinogenesis ,Drug delivery ,Differential pulse voltammetry ,0210 nano-technology - Abstract
Mubashar Rehman,1–3 Ayesha Ihsan,2 Asadullah Madni,1 Sadia Zafar Bajwa,2 Di Shi,3 Thomas J Webster,3,4 Waheed S Khan2 1Department of Pharmacy, The Islamia University of Bahawalpur, Bahawalpur, Punjab, Pakistan; 2Nanobiotech Group, National Institute of Biotechnology and Genetic Engineering, Faisalabad, Punjab, Pakistan; 3Department of Chemical Engineering, Northeastern University, Boston, MA, USA; 4Center of Excellence for Advanced Materials Research, King Abdulaziz University, Jeddah, Saudi Arabia Abstract: Thermoresponsive drug delivery systems are designed for the controlled and targeted release of therapeutic payload. These systems exploit hyperthermic temperatures (>39°C), which may be applied by some external means or due to an encountered symptom in inflammatory diseases such as cancer and arthritis. The objective of this paper was to provide some solid evidence in support of the hypothesis that solid lipid nanoparticles (SLNs) can be used for thermoresponsive targeting by undergoing solid–liquid phase transition at their melting point (MP). Thermoresponsive lipid mixtures were prepared by mixing solid and liquid natural fatty acids, and their MP was measured by differential scanning calorimetry (DSC). SLNs (MP 39°C) containing 5-fluorouracil (5-FU) were synthesized by hot melt encapsulation method, and were found to have spherical shape (transmission electron microscopy studies), desirable size (90% drug was released at 39°C after 5hours, suggesting that the SLNs show thermoresponsive drug release, thus confirming our hypothesis. Drug release from SLNs at 39°C was similar to oleic acid and linoleic acid nanoemulsions used in this study, which further confirmed that thermoresponsive drug release is due to solid–liquid phase transition. Next, a differential pulse voltammetry-based electrochemical chemical detection method was developed for quick and real-time analysis of 5-FU release, which also confirmed thermoresponsive drug release behavior of SLNs. Blank SLNs were found to be biocompatible with human gingival fibroblast cells, although 5-FU-loaded SLNs showed some cytotoxicity after 24hours. 5-FU-loaded SLNs showed thermoresponsive cytotoxicity to breast cancer cells (MDA-MB-231) as cytotoxicity was higher at 39°C (cell viability 72%–78%) compared to 37°C (cell viability >90%) within 1hour. In conclusion, this study presents SLNs as a safe, simple, and effective platform for thermoresponsive targeting. Keywords: temperature sensitive, breast cancer, 5-fluorouracil, nanostructured lipid carriers, emulsions, fatty acids
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- 2017
13. Development of Eudragit RS 100 Microparticles Loaded with Ropinirole: Optimization and In Vitro Evaluation Studies
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Muhammad Ahmad Mahmood, Prince Muhammad Kashif, Asadullah Madni, Muhammad Ashfaq, Nayab Tahir, Mubashar Rehman, and Muhammad Imran Khan
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Materials science ,Indoles ,Kinetics ,Acrylic Resins ,Drug Evaluation, Preclinical ,Pharmaceutical Science ,02 engineering and technology ,Aquatic Science ,Pharmacology ,030226 pharmacology & pharmacy ,Eudragit RS ,03 medical and health sciences ,0302 clinical medicine ,X-Ray Diffraction ,Drug Discovery ,medicine ,Particle Size ,Ecology, Evolution, Behavior and Systematics ,chemistry.chemical_classification ,Ecology ,General Medicine ,Polymer ,021001 nanoscience & nanotechnology ,Box–Behnken design ,In vitro ,Microspheres ,Ropinirole ,Chemical engineering ,chemistry ,Delayed-Action Preparations ,Particle size ,0210 nano-technology ,Agronomy and Crop Science ,medicine.drug ,Fire retardant - Abstract
The current study aimed to develop novel pH independent microparticles loaded with ropinirole (ROP) for sustained drug release. Eudragit RS 100 was used as release retardant and microparticles were fabricated by oil-in-oil emulsion solvent evaporation method. A three-factor three-level Box-Behnken design using Design-Expert software was employed to optimize formulation variables. Ropinirole loaded microparticles were evaluated with respect to morphology, particle size, encapsulation efficiency, and in vitro release profile. Optical microscopy and SEM micrographs indicated spherical shape with smooth surface and well-defined boundary. The particle size was in the range of 98.86 to 236.29 μm, being significantly increased with increasing polymer concentration. Higher polymer load also increased the thickness of internal polymer network, which led to reduced drug loss and higher entrapment efficiency (89%). The cumulative in vitro release was found to be in the range of 54.96 to 99.36% during the release studies (12 h) following zero order release kinetics and non-Fickian diffusion pattern. The developed microparticles have the potential to sustain the release of ropinirole, which may lead to a reduction in its adverse effects and improved management of Parkinson’s disease.
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- 2016
14. Solid and liquid lipid-based binary solid lipid nanoparticles of diacerein: in vitro evaluation of sustained release, simultaneous loading of gold nanoparticles, and potential thermoresponsive behavior
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Muhammad Ashfaq, Mubashar Rehman, Ayesha Ihsan, Waheed S. Khan, Sadia Zafar Bajwa, Muhammad Imran Khan, Muhammad Ahmad Mahmood, Asadullah Madni, and Imran Shakir
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Materials science ,binary ,Anti-Inflammatory Agents ,Biophysics ,Metal Nanoparticles ,Pharmaceutical Science ,Nanoparticle ,Anthraquinones ,Bioengineering ,Biosensing Techniques ,In Vitro Techniques ,Biomaterials ,chemistry.chemical_compound ,Drug Delivery Systems ,X-Ray Diffraction ,International Journal of Nanomedicine ,Drug Discovery ,Solid lipid nanoparticle ,medicine ,Diacerein ,Original Research ,Chromatography ,Fatty Acids ,Organic Chemistry ,Temperature ,Electrochemical Techniques ,General Medicine ,Lipids ,Lauric acid ,body regions ,Kinetics ,Oleic acid ,chemistry ,Colloidal gold ,gold nanoparticles ,Delayed-Action Preparations ,diacerein ,Drug delivery ,nanoparticles ,Gold ,Stearic acid ,thermoresponsive ,Stearic Acids ,medicine.drug - Abstract
Mubashar Rehman,1 Asadullah Madni,1 Ayesha Ihsan,2 Waheed Samraiz Khan,2 Muhammad Imran Khan,1 Muhammad Ahmad Mahmood,1 Muhammad Ashfaq,1 Sadia Zafar Bajwa,2 Imran Shakir31Department of Pharmacy, Faculty of Pharmacy and Alternative Medicine, The Islamia University of Bahawalpur, Pakistan; 2Nanobiotechnology Group, Industrial Biotechnology Division, National Institute of Biotechnology and Genetic Engineering, Faisalabad, Pakistan; 3Sustainable Energy Technologies (SET) centre, College of Engineering, King Saud University, Riyadh, Saudi ArabiaAbstract: Binary fatty acid mixture-based solid lipid nanoparticles (SLNs) were prepared for delivery of diacerein, a novel disease-modifying osteoarthritis drug, with and without simultaneously loaded gold nanoparticles (GNPs). In order to optimize SLNs for temperature-responsive release, lipid mixtures were prepared using different ratios of solid (stearic acid or lauric acid) and liquid (oleic acid) fatty acids. SLNs were prepared by microemulsification (53 nm), hot melt encapsulation (10.4 nm), and a solvent emulsification-evaporation technique (7.8 nm). The physicochemical characteristics of SLNs were studied by Zetasizer, Fourier transform infrared, and X-ray diffraction analysis. High encapsulation of diacerein was achieved with diacerein-loaded and simultaneously GNP-diacerein-loaded SLNs. In vitro dissolution studies revealed a sustained release pattern for diacerein over 72 hours for diacerein-loaded SLNs and 12 hours for GNP-diacerein-loaded SLNs. An increase in diacerein payload increased the release time of diacerein while GNPs decreased it. In addition, rapid release of diacerein over 4 hours was observed at 40°C (melting point of optimized fatty acid mixture), demonstrating that these binary SLNs could be used for thermoresponsive drug delivery. Kinetic modeling indicated that drug release followed zero order and Higuchi diffusion models (R2>0.9), while the Korsmeyer-Peppas model predicted a diffusion release mechanism (n
- Published
- 2015
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