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Your search keyword '"Shunxing Su"' showing total 3 results
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3 results on '"Shunxing Su"'

1. Enzymatic manipulations of DNA oligonucleotides on microgel: towards development of DNA-microgel bioassays

Author

Yingfu Li, M. Monsur Ali, Robert Pelton, Carlos D. M. Filipe, and Shunxing Su

Subjects
DNA Ligases, DNA polymerase, Molecular Sequence Data, Biosensing Techniques, DNA-Directed DNA Polymerase, Polymerase cycling assembly, Catalysis, chemistry.chemical_compound, Materials Chemistry, chemistry.chemical_classification, DNA ligase, biology, Base Sequence, Oligonucleotide, Hybridization probe, fungi, Metals and Alloys, food and beverages, General Chemistry, DNA, Surfaces, Coatings and Films, Electronic, Optical and Magnetic Materials, Real-time polymerase chain reaction, chemistry, Biochemistry, Rolling circle replication, Ceramics and Composites, biology.protein, Biological Assay, DNA Probes, Gels
Abstract

We demonstrate that DNA oligonucleotides covalently coupled to colloidal microgel can be manipulated by T4 DNA ligase for DNA ligation and by Phi29 DNA polymerase for rolling circle amplification (RCA). We also show that the long single-stranded RCA product can generate intensive fluorescence upon hybridization with complementary fluorescent DNA probe. We believe DNA-microgel conjugates can be explored for the development of DNA based bioassays and biosensors.

Published
2007

2. Microgel-Based Inks for Paper-Supported Biosensing Applications.

Author

Shunxing Su, Md. Monsur Ali, Carlos D. M. Filipe, Yingfu Li, and Robert Pelton

Subjects
*DNA, *NUCLEIC acids, *GENES, *BIOSENSORS
Abstract

As a first step for the development of biosensing inks for inexpensive paper-based biodetection, we prepared paper strips printed with carboxylic poly( N-isopropylacrylamide) microgels that were modified either with an antibody or with a DNA aptamer. We found that the antibody and the DNA aptamer retained their recognition capabilities when coupled to microgel. The printed microgel remains stationary during chromatographic elution while the microgel-supported molecular recognition elements are accessible to their intended targets present in the elution solution. Our work indicates that microgels, large enough to isolate the biosensors from the paper surface, are sufficiently hydrophilic to be wetted during chromatographic elution, exposing the gel-supported affinity probes to their targets. [ABSTRACT FROM AUTHOR]

Published
2008
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3. Enzymatic manipulations of DNA oligonucleotides on microgel: towards development of DNA–microgel bioassays.

Author

Md Monsur Ali, Shunxing Su, Carlos D. M. Filipe, Robert Pelton, and Yingfu Li

Subjects
*DEOXYRIBOSE, *DNA, *GENES, *NUCLEIC acids
Abstract

We demonstrate that DNA oligonucleotides covalently coupled to colloidal microgel can be manipulated by T4 DNA ligase for DNA ligation and by Phi29 DNA polymerase for rolling circle amplification (RCA). We also show that the long single-stranded RCA product can generate intensive fluorescence upon hybridization with complementary fluorescent DNA probe. We believe DNA–microgel conjugates can be explored for the development of DNA based bioassays and biosensors. [ABSTRACT FROM AUTHOR]

Published
2007
Full Text
View/download PDF

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