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Enzymatic manipulations of DNA oligonucleotides on microgel: towards development of DNA–microgel bioassays.
- Source :
-
Chemical Communications . Oct2007, Vol. 2007 Issue 43, p4459-4461. 3p. - Publication Year :
- 2007
-
Abstract
- We demonstrate that DNA oligonucleotides covalently coupled to colloidal microgel can be manipulated by T4 DNA ligase for DNA ligation and by Phi29 DNA polymerase for rolling circle amplification (RCA). We also show that the long single-stranded RCA product can generate intensive fluorescence upon hybridization with complementary fluorescent DNA probe. We believe DNA–microgel conjugates can be explored for the development of DNA based bioassays and biosensors. [ABSTRACT FROM AUTHOR]
- Subjects :
- *DEOXYRIBOSE
*DNA
*GENES
*NUCLEIC acids
Subjects
Details
- Language :
- English
- ISSN :
- 13597345
- Volume :
- 2007
- Issue :
- 43
- Database :
- Academic Search Index
- Journal :
- Chemical Communications
- Publication Type :
- Academic Journal
- Accession number :
- 27314714
- Full Text :
- https://doi.org/10.1039/b709817k