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Enzymatic manipulations of DNA oligonucleotides on microgel: towards development of DNA–microgel bioassays.

Authors :
Md Monsur Ali
Shunxing Su
Carlos D. M. Filipe
Robert Pelton
Yingfu Li
Source :
Chemical Communications. Oct2007, Vol. 2007 Issue 43, p4459-4461. 3p.
Publication Year :
2007

Abstract

We demonstrate that DNA oligonucleotides covalently coupled to colloidal microgel can be manipulated by T4 DNA ligase for DNA ligation and by Phi29 DNA polymerase for rolling circle amplification (RCA). We also show that the long single-stranded RCA product can generate intensive fluorescence upon hybridization with complementary fluorescent DNA probe. We believe DNA–microgel conjugates can be explored for the development of DNA based bioassays and biosensors. [ABSTRACT FROM AUTHOR]

Details

Language :
English
ISSN :
13597345
Volume :
2007
Issue :
43
Database :
Academic Search Index
Journal :
Chemical Communications
Publication Type :
Academic Journal
Accession number :
27314714
Full Text :
https://doi.org/10.1039/b709817k