1. S-540956, a CpG Oligonucleotide Annealed to a Complementary Strand With an Amphiphilic Chain Unit, Acts as a Potent Cancer Vaccine Adjuvant by Targeting Draining Lymph Nodes.
- Author
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Nakagawa T, Tanino T, Onishi M, Tofukuji S, Kanazawa T, Ishioka Y, Itoh T, Kugimiya A, Katayama K, Yamamoto T, Nagira M, and Ishii KJ
- Subjects
- Adjuvants, Vaccine chemistry, Animals, Cell Differentiation, DNA chemistry, Female, Humans, Immunization, Lymphocyte Activation, Mice, Mice, Inbred C57BL, Neoplasms, Experimental, Oligodeoxyribonucleotides chemistry, Surface-Active Agents chemistry, Toll-Like Receptor 9 agonists, Toll-Like Receptor 9 genetics, Vaccines, Subunit, Adjuvants, Vaccine therapeutic use, CD8-Positive T-Lymphocytes immunology, Cancer Vaccines immunology, Dendritic Cells immunology, Lung Neoplasms immunology, Oligodeoxyribonucleotides administration & dosage, Papillomavirus E7 Proteins immunology, Sentinel Lymph Node immunology, Toll-Like Receptor 9 metabolism
- Abstract
Robust induction of cancer-antigen-specific CD8
+ T cells is essential for the success of cancer peptide vaccines, which are composed of a peptide derived from a cancer-specific antigen and an immune-potentiating adjuvant, such as a Toll-like receptor (TLR) agonist. Efficient delivery of a vaccine antigen and an adjuvant to antigen-presenting cells in the draining lymph nodes (LNs) holds key to maximize vaccine efficacy. Here, we developed S-540956, a novel TLR9-agonistic adjuvant consisting of B-type CpG ODN2006 (also known as CpG7909), annealed to its complementary sequence oligodeoxynucleotide (ODN) conjugated to a lipid; it could target both a cancer peptide antigen and a CpG-adjuvant in the draining LNs. S-540956 accumulation in the draining LNs and activation of plasmacytoid dendritic cells (pDCs) were significantly higher than that of ODN2006. Mechanistic analysis revealed that S-540956 enhanced the induction of MHC class I peptide-specific CD8+ T cell responses via TLR9 in a CD4+ T cell-independent manner. In mice, the therapeutic effect of S-540956-adjuvanted with a human papillomavirus (HPV)-E7 peptide vaccine against HPV-E7-expressing TC-1 tumors was significantly better than that of an ODN2006-adjuvanted vaccine. Our findings demonstrate a novel adjuvant discovery with the complementary strand conjugated to a lipid, which enabled draining LN targeting and increased ODN2006 accumulation in draining LNs, thereby enhancing the adjuvant effect. Our findings imply that S-540956 is a promising adjuvant for cancer peptide vaccines and has a high potential for applications in various vaccines, including recombinant protein vaccines., Competing Interests: TN, TT, MO, ST, TK, YI, TI, AK, KK, and MN are employees of Shionogi & Co., Ltd. The remaining authors declare that the research was conducted in the absence of any commercial or financial relationships that could be construed as a potential conflict of interest. The authors declare that this study received funding from Shionogi & Co., Ltd. The funder had the following involvement in the study: study design, collection, analysis, interpretation of data, the writing of this article or the decision to submit it for publication., (Copyright © 2021 Nakagawa, Tanino, Onishi, Tofukuji, Kanazawa, Ishioka, Itoh, Kugimiya, Katayama, Yamamoto, Nagira and Ishii.)- Published
- 2021
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