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TANK-binding kinase 1-dependent or -independent signaling elicits the cell-type-specific innate immune responses induced by the adenovirus vector.
- Source :
-
International immunology [Int Immunol] 2016 Mar; Vol. 28 (3), pp. 105-15. Date of Electronic Publication: 2015 Oct 20. - Publication Year :
- 2016
-
Abstract
- Adenovirus vectors (Adv) elicit innate immune responses via several pattern-recognition receptors. Although it has been suggested that various Adv-induced mechanisms play important roles in the induction of innate immunity in vitro, the impacts of these mechanisms in vivo remain unclear. Viral nucleic acids elicit innate immune responses through the recognition of cytosolic nucleic acid sensors and transduce intracellular signals to TANK-binding kinase (TBK) 1. In this study, to determine the impacts of viral nucleic acids on innate immune responses in vivo, we administered transgene-expressing Adv to Tbk1-deficient mice. The systemic Adv administration failed to induce type I interferons (type I IFNs) in the spleen, but not the liver, of Tbk1-deficient mice, resulting in the increase of transgene-expressing cells in the spleen, but not the liver. Moreover, Adv failed to induce type I IFNs in the bone-marrow-derived dendritic cells, but not the mouse embryonic fibroblasts, from Tbk1-deficient mice in vitro. These results support the idea that Adv elicit innate immunity in immune cells and non-immune cells in a TBK1-dependent and TBK1-independent manner, respectively.<br /> (© The Japanese Society for Immunology. 2015. All rights reserved. For permissions, please e-mail: journals.permissions@oup.com.)
- Subjects :
- Animals
Cells, Cultured
DNA, Viral immunology
Dendritic Cells virology
Fibroblasts virology
Immunity, Innate
Interferon Type I metabolism
Liver immunology
Mice
Mice, Inbred C57BL
Mice, Knockout
Protein Serine-Threonine Kinases genetics
Signal Transduction genetics
Spleen immunology
Adenoviridae immunology
Dendritic Cells immunology
Fibroblasts immunology
Genetic Vectors immunology
Liver virology
Organ Specificity genetics
Protein Serine-Threonine Kinases metabolism
Spleen virology
Subjects
Details
- Language :
- English
- ISSN :
- 1460-2377
- Volume :
- 28
- Issue :
- 3
- Database :
- MEDLINE
- Journal :
- International immunology
- Publication Type :
- Academic Journal
- Accession number :
- 26489883
- Full Text :
- https://doi.org/10.1093/intimm/dxv058