1. Phenotypic and molecular characterisation of Salmonella spp. isolates in healthy poultry.
- Author
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Lucca V, Borges KA, Furian TQ, Chitolina GZ, Streck AF, da Rocha DT, de Souza Moraes HL, and Nascimento VP
- Subjects
- Animals, Drug Resistance, Multiple, Bacterial, Biofilms, Phenotype, Virulence, Salmonella genetics, Salmonella physiology, Salmonella drug effects, Salmonella isolation & purification, Microbial Sensitivity Tests veterinary, Electrophoresis, Gel, Pulsed-Field veterinary, Serogroup, Salmonella Infections, Animal microbiology, Salmonella Infections, Animal epidemiology, Chickens microbiology, Poultry Diseases microbiology, Poultry Diseases epidemiology, Salmonella enterica genetics, Salmonella enterica physiology, Salmonella enterica drug effects, Salmonella enterica isolation & purification, Anti-Bacterial Agents pharmacology
- Abstract
1. Epidemiological surveillance of Salmonella spp. serves as a primary tool for maintaining the health of poultry flocks. Characterising circulating serotypes is crucial for implementing control and prevention measures. This study conducted phenotypic and molecular characterisation of S. enterica Pullorum, S. enterica Heidelberg, and S. enterica Corvalis isolated from broiler chickens during slaughtering.2. All strains were susceptible to gentamicin, neomycin and norfloxacin. However, resistance rates exceeded 50% for ciprofloxacin and tiamulin, irrespective of the serotype. Approximately 64% of strains were classified as multidrug-resistant, with S. enterica Heidelberg strains exhibiting significantly higher overall resistance. The isolates demonstrated the ability to adhere and produce biofilm at a minimum of three temperatures, with S. enterica Pullorum capable of biofilm production at all temperatures encountered during poultry rearing.3. Each strain possessed between two and seven different virulence-associated genes. Genetic similarity, as indicated by pulsed field gel electrophoresis, exceeded 90% for all three serotypes and strains were classified in the R5 ribotype by PCR, regardless of serotype. Sequencing revealed high similarity among all strains, with homology ranging from 99.61 to 100% and all were classified to a single cluster.4. The results suggested a clonal relationship among the strains, indicating the possible circulation of a unique clonal group of S. enterica Pullorum in the southern region of Brazil.
- Published
- 2024
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