1. A novel preparative method for nanoparticle albumin-bound paclitaxel with high drug loading and its evaluation both in vitro and in vivo
- Author
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Jingxue Nai, Zhiping Li, Meiyan Yang, Siyu Ma, Xingguo Mei, Zhenbo Yang, Jinbang Zhang, Yang Yang, Jiaxin Li, Yumei Zhao, Yue Gao, Chunsheng Gao, Wei Gong, Hui Li, Fanglin Yu, and Yu Li Wang
- Subjects
Male ,Cancer Treatment ,Biochemistry ,Polyethylene Glycols ,Freeze-drying ,chemistry.chemical_compound ,Mice ,Neoplasms ,Medicine and Health Sciences ,Nanotechnology ,Tissue Distribution ,Drug Carriers ,Multidisciplinary ,Chemistry ,Organic Compounds ,Pharmaceutics ,Human serum albumin ,Paclitaxel ,Oncology ,Physical Sciences ,Medicine ,Engineering and Technology ,medicine.drug ,Research Article ,Half-Life ,Drug Administration ,Science ,Materials Science ,Material Properties ,Transplantation, Heterologous ,Mice, Nude ,Antineoplastic Agents ,Polyethylene glycol ,Pharmacokinetics ,Drug Therapy ,In vivo ,Albumins ,Cell Line, Tumor ,PEG ratio ,medicine ,Animals ,Humans ,Particle Size ,Rats, Wistar ,Pharmacology ,Chromatography ,Ethanol ,Organic Chemistry ,Albumin ,Chemical Compounds ,Cancers and Neoplasms ,Biology and Life Sciences ,Proteins ,Rats ,Drug Liberation ,Solubility ,Alcohols ,Nanoparticles ,Albumin-Bound Paclitaxel - Abstract
We developed a novel preparative method for nanoparticle albumin-bound (nab) paclitaxel with high drug loading, which was based on improved paclitaxel solubility in polyethylene glycol (PEG) and self-assembly of paclitaxel in PEG with albumin powders into nanoparticles. That is, paclitaxel and PEG were firstly dissolved in ethanol, which was subsequently evaporated under vacuum. The obtained liquid was then mixed with human serum albumin powders. Thereafter, the mixtures were added into phosphate-buffered saline and nab paclitaxel suspensions emerged after ultrasound. Nab paclitaxel was finally acquired after dialysis and freeze drying. The drug loading of about 15% (W/V) were realized in self-made nab paclitaxel, which was increased by approximately 50% compared to 10% (W/V) in Abraxane. Now this new preparative method has been authorized to obtain patent from China and Japan. The similar characteristics of self-made nab paclitaxel compared to Abraxane were observed in morphology, encapsulation efficiency, in vitro release, X-ray diffraction analysis, differential scanning calorimetry analysis, and circular dichroism spectra analysis. Consistent concentration-time curves in rats, biodistributions in mice, anti-tumor activities in mice, and histological transmutation in mice were also found between Abraxane and self-made nanoparticles. In a word, our novel preparative method for nab paclitaxel can significantly improve drug loading, obviously decrease product cost, and is considered to have potent practical value.
- Published
- 2021