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Differential and sequential delivery of fluorescent lysosomal probes into phagosomes in mouse peritoneal macrophages
- Source :
- The Journal of Cell Biology
- Publication Year :
- 1987
- Publisher :
- The Rockefeller University Press, 1987.
-
Abstract
- It has previously been inferred that the fusion of a macrophage secondary lysosome with a phagosome delivers the entire lysosomal contents uniformly to the phagosome. We found, however, that different fluorescent lysosomal probes can enter phagosomes at remarkably different rates, even though they are initially sequestered together in the same organelles. Thus, sulforhodamine is almost exclusively delivered to yeast-containing phagosomes within 2 h of phagocytosis. But fluoresceinated, high molecular weight dextran accumulates in the same phagosomes only over a period of approximately 24 h. We postulate that the delivery of lysosomal contents may involve an intermittent and incremental process in which individual components can be selectively and sequentially transferred.
- Subjects :
- Phagocytosis
Biology
chemistry.chemical_compound
Peritoneal cavity
Mice
Lysosome
Phagosomes
Organelle
medicine
Macrophage
Animals
Fluorescein
Peritoneal Cavity
Phagosome
Rhodamines
Macrophages
Dextrans
Cell Biology
Articles
Fluoresceins
Cell biology
Dextran
medicine.anatomical_structure
chemistry
Biochemistry
Microscopy, Fluorescence
Xanthenes
Lysosomes
Subjects
Details
- Language :
- English
- ISSN :
- 15408140 and 00219525
- Volume :
- 104
- Issue :
- 6
- Database :
- OpenAIRE
- Journal :
- The Journal of Cell Biology
- Accession number :
- edsair.doi.dedup.....483086cc159e14cbe43630675f0bf87c