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Cancer metastasis arises from magical pathological cell migration. An accurate and rapid evaluation of cell migration can reduce the number of animals used in experiments to develop a new drug to restrain the metastasis. In this work, high-strength double network hydrogels were synthesized to develop the three-dimensional (3D) scaffolds, which were applied to screen drug of anticancer in vitro. Compression strength was examined for Poly(2-acrylamido-2-methyl-1-propanesulfonic acid)/ Polyacrylamide (PAMPS/PAAm) double network hydrogel, Polyethylene glycol-Poly(2-acrylamido-2-methyl-1-propanesulfonic acid)/ Polyacrylamide (PEG-PAMPS/PAAm) double network hydrogel and Poly(vinyl alcohol)-Poly(2-acrylamido-2-methyl-1-propanesulfonic acid)/ Polyacrylamide (PVA-PAMPS/PAAm) double network hydrogel in more than 90% deformation rate. The results showed that PEG-PAMPS/PAAm hydrogel and PVA-PAMPS/PAAm hydrogel could provide high mechanical property to afford the scaffold durability and simulate cell metastasis under a simulated blood pressure. Used as the in vitro scaffold, the PEG-PAMPS/PAAm hydrogel and PVA-PAMPS/PAAm hydrogel were co-cultured with the two cancer cell, NB4 cell and A549 cell, which is the acute promyelocytic leukemia cell line and the human lung cancer cell, respectively. The results showed that the hydrogels had no intrinsic cytotoxicity to the two cells. Furthermore, under adriamycin treatment, the NB4 cell migration in two-dimensional (2D) polycarbonate membranes and PEG-PAMPS/PAAm hydrogel scaffold was investigated. The results indicated that the PEG-PAMPS/PAAm hydrogel had inter-connective porous structures and hole walls with deformability, could provide more practical channels to decrease the damage of the amoeboid migrated cells. This paper provides a 3D scaffold for studying cell migration and thus finds a rapid in vitro evaluation of a new drug to restrain the cancer cell metastasis.

This study aims to design and synthesize a series of N-Acyl-N-(m-fluoro- benzyl)-6- amino-coumarins through the principle of active substructure stitching, which are based on the core structure of N-(m-fluoro-benzyl)-6-amino-coumarin. The structures of target compounds e1–e25 have been characterized by 1H NMR, 13C NMR, ESI-MS and elemental analysis. Meanwhile, their agricultural activity have been evaluated in two weeds (Amaranth and Crabgrass) and four widespread noxious pathogens (V.mali, B.cinerea, F.axysporium and C.bacteria). The herbicidal activity results showed that almost all synthetic molecules have a greater impact on the stem system than on the root. Excellent inhibition rates were discovered from compounds e2–e5 and e20–e23 against Amaranth on stems, which were above 58%(20 mg/L), 68%(100 mg/L) respectively. Compounds e2 and e21 also exhibited striking inhibition on stems growth of both weeds. Anti-pathogenic activity showed that all the compounds exerted a better inhibitory activity on B.cinerea at 20 ppm compared to control carbendazim. All the heterocyclic substituted compounds (e17–e24, >57%) made a better influence than the control (54.1%) at the100 ppm. This research provides promising herbicidal and anti-pathogenic agents that have the better effects and can be potential for further development.

Thermal degradation of hydroxypropyl trimethyl ammonium chloride chitosan–Cd complexes (HTCC–Cd) was investigated by thermogravimetric analysis. The results indicate that the degradation of HTCC–Cd in nitrogen atmosphere was two-step reaction. For the first step of degradation, the initial temperature of mass loss (T 0), the final temperature of mass loss (T f), and the temperature of maximum mass loss (T p) increase linearly with the rising of heating rate (B). T o = 1.241B + 220.3, T p = 1.111B + 245.8, and T f = 1.335B + 358.2. Using different methods, the kinetic parameters of the two steps were investigated. The results show that the activation energies of the first step of degradation obtained using Friedman and Flynn–Wall–Ozawa methods are 1.684 × 105 and 1.646 × 105 J mol−1, and the corresponding activation energies for the second step are 1.165 × 105 J mol−1 and 1.373 × 105 kJ mol−1. The results obtained from Phadnis–Deshpande methods indicate that the two degradation processes are both nucleation and growth process, and follow A4 mechanism with intergral form g(X) = [−ln(1 − X)]4.

N-(2-hydroxyl) propyl-3-trimethyl ammonium chitosan chloride (HTCC) is hydro-soluble chitosan (CS) derivative, which can be obtained by the reaction between epoxypropyl trimethyl ammonium chloride (ETA) and CS. The preparation parameters for the synthesis of HTCC were optimized by orthogonal experimental design. ETA was successfully grafted into the free amino group of CS. Grafting of ETA with CS had great effect on the crystal structure of HTCC, which was confirmed by the XRD results. HTCC displayed higher capability to form nanoparticles by crosslinking with negatively charged sodium tripolyphosphate (TPP). Ribavrin- (RIV-) loaded HTCC nanoparticles were positively charged and were spherical in shape with average particle size of 200 nm. More efficient drug encapsulation efficiency and loading capacity were obtained for HTCC in comparison with CS, however, HTCC nanoparticles displayed faster release rate due to its hydro-soluble properties. The results suggest that HTCC is a promising CS derivative for the encapsulation of hydrophilic drugs in obtaining sustained release of drugs.

The present work reports the first solid phase synthesis of biologically interesting d - threo -1-phenyl-2-decanoylamino-3-morpholino-1-propanol ( d - threo -PDMP) derivatives. This synthetic strategy includes facile preparation of versatile azido intermediate ( 5 ) in a relatively short sequence and the subsequent derivatization of 5 , which led to a series of sulfonamide, urea and heterocycle substituted PDMP analogs ( 10 and 10 ′). With this method, a 5280-member compound library has been successfully built by IRORI Nanokan © system.

The thermal degradation of quaternized chitosan–cupric ion compounds in nitrogen was studied by thermogravimetry analysis (TG) and differential thermal analysis (DTA). The effect of cupric ions on the thermal degradation behaviors of quaternized chitosan was discussed. Fourier transform-infrared (FTIR) and X-ray diffraction (XRD) were utilized to determine the micro-structure of quaternized chitosan–cupric ion compounds. The results of FTIR and XRD show that there are coordinating bonds between quaternized chitosan and cupric ions. Thermal analysis indicates that the thermal degradation of quaternary chitosan–cupric ion compounds is a two-stage reaction, in which there are the decomposition of N–C2 bond on the side-chain of quaternized chitosan and the cleavage of glycosidic linkages of chitosan. The two reactions are exothermic. The impact of cupric ion on the thermal degradation of quaternized chitosan is significant, and the temperature and activation energy of the degradation are related to the weight fraction of the cupric ion.

In the title complex, [Zn(C8H9N2O4)2(H2O)2]·3.5H2O, the ZnII ion is coordinated by two N,O-bidentate H2pimda ligands (H3pimda = 2-propyl-1H-imidazole-4,5-dicarb­oxy­lic acid) and two water mol­ecules in a distorted octa­hedral environment. In the crystal structure, extensive inter­molecular O—H⋯O and N—H⋯O hydrogen bonds stabilize the three-dimensional supra­molecular network. Intra­molecular O—H⋯O hydrogen bonds between the carboxyl groups are also observed. The propyl groups of the two H2pimda ligands are disordered each over two sites, with occupancy factors of 0.752 (5):0.248 (5) and 0.519 (7):0.481 (7). One of the water mol­ecules is half-occupied.

In the title complex, [Cd(C(8)H(9)N(2)O(4))(2)(H(2)O)(2)]·3.5H(2)O, the Cd(II) is coordinated by two water mol-ecules and N,O-chelated by two 5-carb-oxy-2-propyl-1H-imidazole-4-carboxyl-ate anions in a distorted octa-hedral geometry. The two imidazole rings are oriented to each other with a dihedral angle of 75.1 (2)°. Strong O-H⋯O hydrogen bonds between protonated and deprotonated carboxyl-ate groups occur in the mol-ecular structure. In the crystal structure extensive O-H⋯O and N-H⋯O hydrogen bonds help to stabilize the three-dimensional supra-molecular framework. The propyl groups of anions are disordered over two sites with refined occupancies of 0.768 (6):0.232 (6) and 0.642 (8):0.358 (8).