Search

Your search keyword '"Longting, Si"' showing total 3 results
Start Over Author "Longting, Si" Topic chemistry
3 results on '"Longting, Si"'

1. A putative positive feedback regulation mechanism in CsACS2 expression suggests a modified model for sex determination in cucumber (Cucumis sativus L.)

Author

Shu Wang, Longting Si, Qianyi Tao, Run Cai, Zhen-Hui Gong, Junsong Pan, and Zheng Li

Subjects
one-hormone hypothesis, Ethylene, DNA, Plant, Physiology, Transgene, Molecular Sequence Data, sex determination, Cucumis sativus L, Gene Expression, Flowers, Plant Science, Models, Biological, ethylene induction, chemistry.chemical_compound, positive feedback regulation, Organophosphorus Compounds, Plant Growth Regulators, Gene Expression Regulation, Plant, Transcription (biology), Tobacco, Gene expression, Botany, Promoter Regions, Genetic, Gene, Plant Proteins, Gametogenesis, Plant, Regulation of gene expression, Base Sequence, biology, CsACS2, Sequence Analysis, DNA, Ethylenes, Plants, Genetically Modified, biology.organism_classification, Cell biology, Plant Leaves, Phenotype, chemistry, Seedlings, Silver Nitrate, Plant hormone, Cucumis sativus, Cucumis, Research Paper
Abstract

It is well established that the plant hormone ethylene plays a key role in cucumber sex determination. Since the unisexual control gene M was cloned and shown to encode an ethylene synthase, instead of an ethylene receptor, the 'one-hormone hypothesis', which was used to explain the cucumber sex phenotype, has been challenged. Here, the physiological function of CsACS2 (the gene encoded by the M locus) was studied using the transgenic tobacco system. The results indicated that overexpression of CsACS2 increased ethylene production in the tobacco plant, and the native cucumber promoter had no activity in transgenic tobacco (PM). However, when PM plants were treated with exogenous ethylene, CsACS2 expression could be detected. In cucumber, ethylene treatment could also induce transcription of CsACS2, while inhibition of ethylene action reduced the expression level. These findings suggest a positive feedback regulation mechanism for CsACS2, and a modified 'one-hormone hypothesis' for sex determination in cucumber is proposed.

Published
2012

2. Molecular isolation of the M gene suggests that a conserved-residue conversion induces the formation of bisexual flowers in cucumber plants

Author

Shiqiang Liu, Huiming Chen, Zhonghua Zhang, Qianyi Tao, Weiwei Zhang, Qiuxiang Shi, Lihuang Zhu, Run Cai, Junsong Pan, Longting Si, Zhiqi Jia, Sanwen Huang, and Zheng Li

Subjects
Molecular Sequence Data, Flowers, medicine.disease_cause, Genes, Plant, Conserved sequence, Notes, Genetics, medicine, Allele, Cloning, Molecular, 1-aminocyclopropane-1-carboxylate synthase, Gene, Alleles, Conserved Sequence, Plant Proteins, Cloning, chemistry.chemical_classification, Mutation, ATP synthase, biology, Base Sequence, Enzyme, chemistry, biology.protein, Cucumis sativus
Abstract

Sex determination in plants involves a variety of mechanisms. Here, we report the map-based cloning and characterization of the unisexual-flower-controlling gene M. M was identified as a previously characterized putative 1-aminocyclopropane-1-carboxylic acid synthase gene, while the m allele that mutated at a conserved site (Gly33Cys) lost activity in the original enzymatically active allele.

Published
2009

3. Development and fine mapping of three co-dominant SCAR markers linked to the M/m gene in the cucumber plant (Cucumis sativus L.)

Author

Run Cai, Longting Si, Huanle He, Zheng Li, Qianyi Tao, Yuan Guan, and Junsong Pan

Subjects
Genetic Markers, Chromosomes, Artificial, Bacterial, DNA, Plant, Population, Locus (genetics), Genes, Plant, chemistry.chemical_compound, Chromosome Walking, Gene mapping, Molecular marker, Genetics, Primer walking, education, education.field_of_study, Bacterial artificial chromosome, Polymorphism, Genetic, biology, Bulked segregant analysis, General Medicine, Sequence Analysis, DNA, biology.organism_classification, chemistry, Cucumis sativus, Agronomy and Crop Science, Cucumis, Biotechnology
Abstract

Owing to its diverse sex types, the cucumber plant has been studied widely as a model for sex determination. In addition to environmental factors and plant hormones, three major genes—F/f, M/m, and A/a—regulate the sex types in the cucumber plant. By combining the bulked segregant analysis (BSA) and the sequence-related amplified polymorphism (SRAP) technology, we identified eight markers linking to the M/m locus. Among them, the two closely linked SRAP markers flanking the M/m locus were the co-dominant marker ME1EM26 and the dominant marker ME1EM23. Further, the co-dominant marker ME8SA7 co-segregated with the M/m locus. With the chromosome walking method using the cucumber genomic bacterial artificial chromosome (BAC) library, we successfully developed a co-dominant SCAR marker S_ME1EM23 from the ME1EM23 sequence. Along with the other two co-dominant SCAR markers S_ME1EM26 and S_ME8SA7 (developed from ME1EM26 and ME8SA7, respectively) in a larger segregating population (900 individuals), the M/m locus was mapped between S_ME1EM26 (5.4 cM) and S_ME1EM23 (0.7 cM), and S_ME8SA7 co-segregated with it.

Published
2008

Catalog

Books, media, physical & digital resources
See catalog results