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A putative positive feedback regulation mechanism in CsACS2 expression suggests a modified model for sex determination in cucumber (Cucumis sativus L.)
- Source :
- Journal of Experimental Botany
- Publication Year :
- 2012
- Publisher :
- Oxford University Press (OUP), 2012.
-
Abstract
- It is well established that the plant hormone ethylene plays a key role in cucumber sex determination. Since the unisexual control gene M was cloned and shown to encode an ethylene synthase, instead of an ethylene receptor, the 'one-hormone hypothesis', which was used to explain the cucumber sex phenotype, has been challenged. Here, the physiological function of CsACS2 (the gene encoded by the M locus) was studied using the transgenic tobacco system. The results indicated that overexpression of CsACS2 increased ethylene production in the tobacco plant, and the native cucumber promoter had no activity in transgenic tobacco (PM). However, when PM plants were treated with exogenous ethylene, CsACS2 expression could be detected. In cucumber, ethylene treatment could also induce transcription of CsACS2, while inhibition of ethylene action reduced the expression level. These findings suggest a positive feedback regulation mechanism for CsACS2, and a modified 'one-hormone hypothesis' for sex determination in cucumber is proposed.
- Subjects :
- one-hormone hypothesis
Ethylene
DNA, Plant
Physiology
Transgene
Molecular Sequence Data
sex determination
Cucumis sativus L
Gene Expression
Flowers
Plant Science
Models, Biological
ethylene induction
chemistry.chemical_compound
positive feedback regulation
Organophosphorus Compounds
Plant Growth Regulators
Gene Expression Regulation, Plant
Transcription (biology)
Tobacco
Gene expression
Botany
Promoter Regions, Genetic
Gene
Plant Proteins
Gametogenesis, Plant
Regulation of gene expression
Base Sequence
biology
CsACS2
Sequence Analysis, DNA
Ethylenes
Plants, Genetically Modified
biology.organism_classification
Cell biology
Plant Leaves
Phenotype
chemistry
Seedlings
Silver Nitrate
Plant hormone
Cucumis sativus
Cucumis
Research Paper
Subjects
Details
- ISSN :
- 14602431 and 00220957
- Volume :
- 63
- Database :
- OpenAIRE
- Journal :
- Journal of Experimental Botany
- Accession number :
- edsair.doi.dedup.....aebfa75b94a3ade05057b31976472313