876 results on '"Bergamini A"'
Search Results
2. Dual-Target Additively Manufactured Electrochemical Sensor for the Multiplexed Detection of Protein A29 and DNA of Human Monkeypox Virus
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Luiz Ricardo G. Silva, Jéssica S. Stefano, Cristiane Kalinke, Robert D. Crapnell, Laís C. Brazaca, Luiz H. Marcolino-Junior, Marcio F. Bergamini, Craig E. Banks, and Bruno C. Janegitz
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Chemistry ,QD1-999 - Published
- 2024
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3. Cytomegalovirus in Adenoma and Carcinoma Lesions: Detecting Mono-Infection and Co-Infection in Salivary Glands
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Ana Carolina Silva Guimarães, Jéssica Vasques Raposo Vedovi, Camilla Rodrigues de Almeida Ribeiro, Katrini Guidolini Martinelli, Marcelo Pelajo Machado, Pedro Paulo de Abreu Manso, Barbara Cristina Euzebio Pereira Dias de Oliveira, Mariana Lobo Bergamini, Catharina Simioni de Rosa, Tania Regina Tozetto-Mendoza, Ana Carolina Mamana Fernandes de Souza, Marília Trierveiler Martins, Paulo Henrique Braz-Silva, and Vanessa Salete de Paula
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HCMV ,salivary glands ,co-infections ,Biology (General) ,QH301-705.5 ,Chemistry ,QD1-999 - Abstract
Salivary glands’ neoplasms are hard to diagnose and present a complex etiology. However, several viruses have been detected in these neoplasms, such as HCMV, which can play a role in certain cancers through oncomodulation. The co-infections between HCMV with betaherpesviruses (HHV-6 and HHV-7) and polyomaviruses (JCV and BKV) has been investigated. The aim of the current study is to describe the frequency of HCMV and co-infections in patients presenting neoplastic and non-neoplastic lesions, including in the salivary gland. Multiplex quantitative polymerase chain reaction was used for betaherpesvirus and polyomavirus quantification purposes after DNA extraction. In total, 50.7% of the 67 analyzed samples were mucocele, 40.3% were adenoma pleomorphic, and 8.9% were mucoepidermoid carcinoma. Overall, 20.9% of samples presented triple-infections with HCMV/HHV-6/HHV-7, whereas 9.0% were co-infections with HCMV/HHV-6 and HCMV/HHV-7. The largest number of co-infections was detected in pleomorphic adenoma cases. All samples tested negative for polyomaviruses, such as BKV and JCV. It was possible to conclude that HCMV can be abundant in salivary gland lesions. A high viral load can be useful to help better understand the etiological role played by viruses in these lesions. A lack of JCV and BKV in the samples analyzed herein does not rule out the involvement of these viruses in one or more salivary gland lesion subtypes.
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- 2024
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4. Multifunctional Biotechnological Lip Moisturizer for Lip Repair and Hydration: Development, In Vivo Efficacy Assessment and Sensory Analysis
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Débora Dahmer, Sara Scandorieiro, Briani Gisele Bigotto, Thays Amélio Bergamini, Jennifer Germiniani-Cardozo, Isabela Mazarim da Costa, Renata Katsuko Takayama Kobayashi, Gerson Nakazato, Dionísio Borsato, Sandra Helena Prudencio, Marina Leite Mitterer Daltoé, Maria Antonia Pedrine Colabone Celligoi, and Audrey Alesandra Stinghen Garcia Lonni
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lip moisturizer ,levan ,sophorolipid ,Citrus paradisi essential oil ,bioimpedance ,sensory attributes ,Chemistry ,QD1-999 - Abstract
The demand for sustainable cosmetics leads to the search for natural and biotechnological ingredients. The present study reports the development of a multifunctional lip moisturizer containing levan (LEV) from Bacillus subtilis natto, sophorolipids (SOPs) from Starmerella bombicola and Citrus paradisi (OCP) essential oil, using a simplex-centroid experimental design. The formulations were evaluated physicochemically, pharmacotechnically and by DPPH assay. The optimized formulation was selected through the Response Surface Method, and the evaluation of its efficiency in lip hydration was carried out using the bioimpedance method and sensory analysis. The formulations showed pH compatibility with lips and remained stable after a centrifuge test and thermal stress. Spreadability varied between 415.3 and 1217.1 mm2, moisture retention was above 95% and antioxidant capacity was around 50% for all formulations. The optimized formulation, containing 0.4% LEV and 0.8% SOF, maintained the lip hydration already shown by the participants; 85% of them reported improvement in this aspect. For the first time, LEV and SOP were incorporated in lip moisturizers, which is an environmentally friendly product with marketing potential. Furthermore, the use of the Skin Analyzer Digital equipment, a low-cost and non-invasive technique, to evaluate the effectiveness of lip products is innovative; this methodology may help in the development of future cosmetology studies.
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- 2023
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5. Waterborne asbestos: Good practices for surface waters analyses
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Chiara Avataneo, Silvana Capella, Mariagrazia Luiso, Giuliana Marangoni, Manuela Lasagna, Domenico A. De Luca, Massimo Bergamini, Elena Belluso, and Francesco Turci
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asbestos analysis ,waterborne asbestos ,surface waters ,guidelines ,analytical methods ,Chemistry ,QD1-999 - Abstract
Asbestos occurrence has been mainly monitored in air so far and only limitedly considered in other matrices, such as water. Waterborne asbestos could originate from natural or anthropogenic sources, leading to non-conventional exposure scenarios. It could be a secondary source of airborne asbestos in case of water-to-air migration, particularly in case of surface moving water, such as in rivers and streams. The scarce attention dedicated to waterborne asbestos has led to a considerable fragmentation in regulatory approaches regarding the study of water samples possibly contaminated by mineral fibres. In this context, this study has been designed to test the reliability of an existing analytical method devoted to natural waters investigations. Following the operational protocol issued by the Piedmont (Italy) Environmental Protection Agency, Scanning Electron Microscopy analyses have been performed on a standard sample of waterborne chrysotile, mimicking stream water. The investigations have been performed by different operators and using different analytical setups, to verify whether the method applied has a good interlaboratory reproducibility and which could be the most error-prone analytical steps. Three data sets have been obtained on the same sample, showing a low reproducibility among each other. Possible reasons causing this discrepancy have been discussed in detail and good practices to perform reliable analyses on surface water samples containing asbestos have been proposed to help the regulatory organs to better define analytical protocols.
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- 2023
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6. Rheumatologist’s Perspective on Non-Infectious Uveitis: Patterns from Tertiary Referral Rheumatologic Clinics in Italy
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Paola Triggianese, Mauro Fatica, Francesco Caso, Luisa Costa, Arianna D’Antonio, Marco Tasso, Elisabetta Greco, Paola Conigliaro, Alberto Bergamini, Claudia Fabiani, Luca Cantarini, and Maria Sole Chimenti
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non-infectious uveitis ,spondyloarthritis ,Behçet disease ,disease-modifying antirheumatic drugs ,HLA-B27 ,HLA-B51 ,Biology (General) ,QH301-705.5 ,Chemistry ,QD1-999 - Abstract
Non-infectious uveitis (NIU) can be an early or even the first extra-articular manifestation of systemic rheumatic diseases, or the first one; thus, rheumatologists are often involved in the diagnostic and therapeutic assessment of NIU. We evaluated 130 patients with a diagnosis of NIU who were admitted to two Italian rheumatologic clinics (Tor Vergata University Hospital in Rome, and Federico II University in Naples) from January 2018 to December 2021. Anterior uveitis (AU) occurred in 75.4% of patients, followed by posterior uveitis (PU, 21.5%); acute (54.6%) and recurrent (35.4%) NIU were more documented than chronic NIU (10%), and a bilateral involvement was observed in 38.7% of cases. Half of NIU cases were associated with spondyloarthritis (SpA); the remaining were affected by Behçet disease (BD)-related uveitis (13.9%) and idiopathic NIU (9.2%). HLA-B27+ patients (34.8%) had a higher prevalence of anterior and unilateral NIU (p = 0.005) with acute course (p = 0.04) than HLA-B27– patients. On the contrary, HLA-B51+ patients (19.6%) had mostly PU and bilateral NIU (p < 0.0001) and recurrent course (p = 0.04) than HLA-B51– patients. At the first rheumatologic referral, 117 patients (90%) received systemic treatments. Findings from this study demonstrate that rheumatologic referral has a pivotal role in the diagnostic work-up of NIU and may dramatically influence NIU-treatment strategies.
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- 2023
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7. Cardiac Functional and Structural Abnormalities in a Mouse Model of CDKL5 Deficiency Disorder
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Manuela Loi, Stefano Bastianini, Giulia Candini, Nicola Rizzardi, Giorgio Medici, Valentina Papa, Laura Gennaccaro, Nicola Mottolese, Marianna Tassinari, Beatrice Uguagliati, Chiara Berteotti, Viviana Lo Martire, Giovanna Zoccoli, Giovanna Cenacchi, Stefania Trazzi, Christian Bergamini, and Elisabetta Ciani
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CDKL5 deficiency disorder ,mouse model ,prolonged QTc interval ,heart aging ,mitochondrial dysfunction ,Biology (General) ,QH301-705.5 ,Chemistry ,QD1-999 - Abstract
CDKL5 (cyclin-dependent kinase-like 5) deficiency disorder (CDD) is a severe neurodevelopmental disease that mostly affects girls, who are heterozygous for mutations in the X-linked CDKL5 gene. Mutations in the CDKL5 gene lead to a lack of CDKL5 protein expression or function and cause numerous clinical features, including early-onset seizures, marked hypotonia, autistic features, gastrointestinal problems, and severe neurodevelopmental impairment. Mouse models of CDD recapitulate several aspects of CDD symptomology, including cognitive impairments, motor deficits, and autistic-like features, and have been useful to dissect the role of CDKL5 in brain development and function. However, our current knowledge of the function of CDKL5 in other organs/tissues besides the brain is still quite limited, reducing the possibility of broad-spectrum interventions. Here, for the first time, we report the presence of cardiac function/structure alterations in heterozygous Cdkl5 +/− female mice. We found a prolonged QT interval (corrected for the heart rate, QTc) and increased heart rate in Cdkl5 +/− mice. These changes correlate with a marked decrease in parasympathetic activity to the heart and in the expression of the Scn5a and Hcn4 voltage-gated channels. Interestingly, Cdkl5 +/− hearts showed increased fibrosis, altered gap junction organization and connexin-43 expression, mitochondrial dysfunction, and increased ROS production. Together, these findings not only contribute to our understanding of the role of CDKL5 in heart structure/function but also document a novel preclinical phenotype for future therapeutic investigation.
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- 2023
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8. Natural Astaxanthin Is a Green Antioxidant Able to Counteract Lipid Peroxidation and Ferroptotic Cell Death
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Nicola Rizzardi, Laura Pezzolesi, Chiara Samorì, Federica Senese, Chiara Zalambani, Walter Pitacco, Natalia Calonghi, Christian Bergamini, Cecilia Prata, and Romana Fato
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astaxanthin ,Haematococcus pluvialis ,oxidative stress ,lipoperoxidation ,ferroptosis ,green chemistry ,Biology (General) ,QH301-705.5 ,Chemistry ,QD1-999 - Abstract
Astaxanthin is a red orange xanthophyll carotenoid produced mainly by microalgae but which can also be chemically synthesized. As demonstrated by several studies, this lipophilic molecule is endowed with potent antioxidant properties and is able to modulate biological functions. Unlike synthetic astaxanthin, natural astaxanthin (NAst) is considered safe for human nutrition, and its production is considered eco-friendly. The antioxidant activity of astaxanthin depends on its bioavailability, which, in turn, is related to its hydrophobicity. In this study, we analyzed the water-solubility of NAst and assessed its protective effect against oxidative stress by means of different approaches using a neuroblastoma cell model. Moreover, due to its highly lipophilic nature, astaxanthin is particularly protective against lipid peroxidation; therefore, the role of NAst in counteracting ferroptosis was investigated. This recently discovered process of programmed cell death is indeed characterized by iron-dependent lipid peroxidation and seems to be linked to the onset and development of oxidative-stress-related diseases. The promising results of this study, together with the “green sources” from which astaxanthin could derive, suggest a potential role for NAst in the prevention and co-treatment of chronic degenerative diseases by means of a sustainable approach.
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- 2022
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9. Chemical Wet Oxidation of Carbon Nanotubes for Electrochemical Determination of Methyl Parathion
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Michelle C. Oliveira, Caetano, Fábio R., Papi, Mauricio A. P., Watanabe, Emily Y., Marcolino-Júnior, Luiz H., and Bergamini, Márcio F.
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Infrared spectroscopy -- Electric properties -- Political aspects ,Sensors -- Electric properties -- Political aspects ,Hydroxides -- Electric properties -- Political aspects ,Nanotubes -- Electric properties -- Political aspects ,Oxidation-reduction reaction -- Electric properties -- Political aspects ,Chemistry - Abstract
Surface properties of carbon nanotubes in electrochemical sensors can be dramatically altered depending on chemical groups and structural defects present on the surface. The effect of oxidation on structural integrity of multiwalled carbon nanotubes through acidic (nitric/sulfuric acids) and basic (sodium hydroxide) agents has been studied. Chemical modifications of carbon nanotubes structures were evaluated by Raman, Fourier transform infrared spectroscopy and transmission electronic microscopy. Furthermore, these materials were used to construct sensors for electrochemical determination of pesticide methyl parathion (MP). Under optimized conditions, a calibration curve was obtained for MP determination employing a glassy carbon electrode modified with acidic treated and basic treated carbon nanotubes which showed a linear response ranging from 1.0 x 10.sup.-7 to 3.4 x 10.sup.-5 M with limits of detection of 3.3 x 10.sup.-8 and 3.5 x10.sup.-8 M, respectively. Analytical performance of the electrodes showed similar voltammetric behavior providing good sensitivity for the determination of methyl parathion., Author(s): Michelle C. Oliveira [sup.1] [sup.2], Fábio R. Caetano [sup.1] [sup.2], Mauricio A. P. Papi [sup.1] [sup.2], Emily Y. Watanabe [sup.1] [sup.2], Luiz H. Marcolino-Júnior [sup.1] [sup.2], Márcio F. Bergamini [...]
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- 2020
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10. A Probe-Based qPCR Method, Targeting 16S rRNA Gene, for the Quantification of Paenibacillus larvae Spores in Powdered Sugar Samples
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Elena Carra, Giorgio Galletti, Emanuele Carpana, Federica Bergamini, Giulio Loglio, Filippo Bosi, Stefano Palminteri, and Stefano Bassi
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American foulbrood ,quantitative TaqMan® real-time PCR (qPCR) ,culture method ,Technology ,Engineering (General). Civil engineering (General) ,TA1-2040 ,Biology (General) ,QH301-705.5 ,Physics ,QC1-999 ,Chemistry ,QD1-999 - Abstract
Paenibacillus larvae (P. larvae) is responsible for American foulbrood (AFB), the most severe bacterial disease of honeybees. The enumeration of P. larvae spores in substrates taken from hives allows for the identification of the contamination levels of the colonies, mostly in those with atypical symptoms or with asymptomatic infections; in these cases, it is essential for the effective control of American foulbrood (AFB). In this work we described a new quantitative TaqMan® probe-based real-time PCR (qPCR) assay, targeting the 16S rRNA gene of P. larvae, used for the quantification of P. larvae spores in powdered sugar samples collected from hives, in comparison to the culture. A total of 105 colonies were selected, belonging to 10 apiaries with different levels of infection, located in northern Italy. The proportions of positive colonies was 54% (57/105) with the culture and 66% (69/105) with qPCR. A significant difference between the two methods was found with McNemar’s test (p = 0.02). Out of the 51 positive samples by both methods, 45 showed higher infection by qPCR compared to the culture. A close concordance with the clinical–epidemiological status of the hives was observed by both methods, with higher infection levels found by qPCR.
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- 2022
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11. Simple, fast and inexpensive method for determination of ranitidine hydrochloride based on conductometric measurements
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Eduardo Henrique Bindewald, João Carlos Rosa-Sobrinho, Márcio Fernando Bergamini, and Luiz Humberto Marcolino-Júnior
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Chemistry ,QD1-999 - Abstract
This work aims the development and optimization of an alternative method for ranitidine hydrochloride (RAN-HCl) determination. The proposed method was based on conductometric titration of RAN by precipitation of AgCl solid using a solution of AgNO3 as titrant. It was investigated the possibility of performing the titrations on hydroalcoholic and deionized water medium. A limit of detection of 1.0 mmol L-1 and 0.5 mmol L-1 were achieved for RAN titration in deionized water and in a 75:25 hydroalcoholic mixture, respectively. Such behavior is attributed to the dielectric constant of hydroalcoholic medium, which is lower than aqueous solution, making AgCl more insoluble and improving the resolution of the conductivity curve around the end point. Therefore, it is concluded that the conductometric titration method to determine RAN using AgNO3 as titrant proved to be feasible at low drug concentrations. The statistical calculations for obtained results suggested good precision for the conductometric method. According to t-test, there were no significant differences between found values at a 95% confidence level. Moreover, obtained results showed an excellent performance of the proposed method on quality control of RAN-HCl in generic formulations without any sample pretreatment.
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- 2019
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12. Evidence for Effects of Extracellular Vesicles on Physical, Inflammatory, Transcriptome and Reward Behaviour Status in Mice
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Nagiua Cuomo-Haymour, Hannes Sigrist, Christian Ineichen, Giancarlo Russo, Ursina Nüesch, Felix Gantenbein, Luka Kulic, Irene Knuesel, Giorgio Bergamini, and Christopher Robert Pryce
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inflammation ,extracellular vesicles ,microRNA ,lipopolysaccharide ,chronic social stress ,nucleus accumbens ,Biology (General) ,QH301-705.5 ,Chemistry ,QD1-999 - Abstract
Immune-inflammatory activation impacts extracellular vesicles (EVs), including their miRNA cargo. There is evidence for changes in the EV miRNome in inflammation-associated neuropsychiatric disorders. This mouse study investigated: (1) effects of systemic lipopolysaccharide (LPS) and chronic social stress (CSS) on plasma EV miRNome; and (2) physiological, transcriptional, and behavioural effects of peripheral or central delivered LPS-activated EVs in recipient mice. LPS or CSS effects on the plasma EV miRNome were assessed by using microRNA sequencing. Recipient mice received plasma EVs isolated from LPS-treated or SAL-treated donor mice or vehicle only, either intravenously or into the nucleus accumbens (NAc), on three consecutive days. Bodyweight, spleen or NAc transcriptome and reward (sucrose) motivation were assessed. LPS and CSS increased the expression of 122 and decreased expression of 20 plasma EV miRNAs, respectively. Peripheral LPS-EVs reduced bodyweight, and both LPS-EVs and SAL-EVs increased spleen expression of immune-relevant genes. NAc-infused LPS-EVs increased the expression of 10 immune-inflammatory genes. Whereas motivation increased similarly across test days in all groups, the effect of test days was more pronounced in mice that received peripheral or central LPS-EVs compared with other groups. This study provides causal evidence that increased EV levels impact physiological and behavioural processes and are of potential relevance to neuropsychiatric disorders.
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- 2022
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13. Differential Expression of Serum Extracellular Vesicle miRNAs in Multiple Sclerosis: Disease-Stage Specificity and Relevance to Pathophysiology
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Nagiua Cuomo-Haymour, Giorgio Bergamini, Giancarlo Russo, Luka Kulic, Irene Knuesel, Roland Martin, André Huss, Hayrettin Tumani, Markus Otto, and Christopher R. Pryce
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clinically isolated syndrome ,relapsing–remitting multiple sclerosis ,inflammation ,extracellular vesicles ,microRNA ,biomarker ,Biology (General) ,QH301-705.5 ,Chemistry ,QD1-999 - Abstract
Multiple sclerosis (MS) is a chronic inflammatory autoimmune disease of the central nervous system (CNS). Its first clinical presentation (clinically isolated syndrome, CIS) is often followed by the development of relapsing–remitting MS (RRMS). The periphery-to-CNS transmission of inflammatory molecules is a major pathophysiological pathway in MS. This could include signalling via extracellular vesicle (EV) microRNAs (miRNAs). In this study, we investigated the serum EV miRNome in CIS and RRMS patients and matched controls, with the aims to identify MS stage-specific differentially expressed miRNAs and investigate their biomarker potential and pathophysiological relevance. miRNA sequencing was conducted on serum EVs from CIS-remission, RRMS-relapse, and viral inflammatory CNS disorder patients, as well as from healthy and hospitalized controls. Differential expression analysis was conducted, followed by predictive power and target-pathway analysis. A moderate number of dysregulated serum EV miRNAs were identified in CIS-remission and RRMS-relapse patients, especially relative to healthy controls. Some of these miRNAs were also differentially expressed between the two MS stages and had biomarker potential for patient-control and CIS–RRMS separations. For the mRNA targets of the RRMS-relapse-specific EV miRNAs, biological processes inherent to MS pathophysiology were identified using in silico analysis. Study findings demonstrate that specific serum EV miRNAs have MS stage-specific biomarker potential and contribute to the identification of potential targets for novel, efficacious therapies.
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- 2022
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14. The Roles of Coenzyme Q in Disease: Direct and Indirect Involvement in Cellular Functions
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Francesco Pallotti, Christian Bergamini, Costanza Lamperti, and Romana Fato
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coenzyme Q10 ,ubiquinone-10 ,ubiquinol-10 ,mitochondria ,OxPhos ,LDL ,Biology (General) ,QH301-705.5 ,Chemistry ,QD1-999 - Abstract
Coenzyme Q (CoQ) is a key component of the respiratory chain of all eukaryotic cells. Its function is closely related to mitochondrial respiration, where it acts as an electron transporter. However, the cellular functions of coenzyme Q are multiple: it is present in all cell membranes, limiting the toxic effect of free radicals, it is a component of LDL, it is involved in the aging process, and its deficiency is linked to several diseases. Recently, it has been proposed that coenzyme Q contributes to suppressing ferroptosis, a type of iron-dependent programmed cell death characterized by lipid peroxidation. In this review, we report the latest hypotheses and theories analyzing the multiple functions of coenzyme Q. The complete knowledge of the various cellular CoQ functions is essential to provide a rational basis for its possible therapeutic use, not only in diseases characterized by primary CoQ deficiency, but also in large number of diseases in which its secondary deficiency has been found.
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- 2021
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15. The Role of DAMPS in Burns and Hemorrhagic Shock Immune Response: Pathophysiology and Clinical Issues. Review
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Desirè Pantalone, Carlo Bergamini, Jacopo Martellucci, Giovanni Alemanno, Alessandro Bruscino, Gherardo Maltinti, Maximilian Sheiterle, Riccardo Viligiardi, Roberto Panconesi, Tommaso Guagni, and Paolo Prosperi
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burns ,DAMPs ,alarmin ,trauma ,cytokine production ,shock ,Biology (General) ,QH301-705.5 ,Chemistry ,QD1-999 - Abstract
Severe or major burns induce a pathophysiological, immune, and inflammatory response that can persist for a long time and affect morbidity and mortality. Severe burns are followed by a “hypermetabolic response”, an inflammatory process that can be extensive and become uncontrolled, leading to a generalized catabolic state and delayed healing. Catabolism causes the upregulation of inflammatory cells and innate immune markers in various organs, which may lead to multiorgan failure and death. Burns activate immune cells and cytokine production regulated by damage-associated molecular patterns (DAMPs). Trauma has similar injury-related immune responses, whereby DAMPs are massively released in musculoskeletal injuries and elicit widespread systemic inflammation. Hemorrhagic shock is the main cause of death in trauma. It is hypovolemic, and the consequence of volume loss and the speed of blood loss manifest immediately after injury. In burns, the shock becomes evident within the first 24 h and is hypovolemic-distributive due to the severely compromised regulation of tissue perfusion and oxygen delivery caused by capillary leakage, whereby fluids shift from the intravascular to the interstitial space. In this review, we compare the pathophysiological responses to burns and trauma including their associated clinical patterns.
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- 2021
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16. A New Protocol of Computer-Assisted Image Analysis Highlights the Presence of Hemocytes in the Regenerating Cephalic Tentacles of Adult Pomacea canaliculata
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Giulia Bergamini, Mohamad Ahmad, Marina Cocchi, and Davide Malagoli
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adult regeneration ,apple snail ,blastema ,hemocyte ,gastropod ,image analysis ,Biology (General) ,QH301-705.5 ,Chemistry ,QD1-999 - Abstract
In humans, injuries and diseases can result in irreversible tissue or organ loss. This well-known fact has prompted several basic studies on organisms capable of adult regeneration, such as amphibians, bony fish, and invertebrates. These studies have provided important biological information and helped to develop regenerative medicine therapies, but important gaps concerning the regulation of tissue and organ regeneration remain to be elucidated. To this aim, new models for studying regenerative biology could prove helpful. Here, the description of the cephalic tentacle regeneration in the adult of the freshwater snail Pomacea canaliculata is presented. In this invasive mollusk, the whole tentacle is reconstructed within 3 months. Regenerating epithelial, connective, muscular and neural components are already recognizable 72 h post-amputation (hpa). Only in the early phases of regeneration, several hemocytes are retrieved in the forming blastema. In view of quantifying the hemocytes retrieved in regenerating organs, granular hemocytes present in the tentacle blastema at 12 hpa were counted, with a new and specific computer-assisted image analysis protocol. Since it can be applied in absence of specific cell markers and after a common hematoxylin-eosin staining, this protocol could prove helpful to evidence and count the hemocytes interspersed among regenerating tissues, helping to unveil the role of immune-related cells in sensory organ regeneration.
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- 2021
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17. Coenzyme Q Depletion Reshapes MCF-7 Cells Metabolism
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Wenping Wang, Irene Liparulo, Nicola Rizzardi, Paola Bolignano, Natalia Calonghi, Christian Bergamini, and Romana Fato
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mitochondrial dysfunction ,glycolysis ,bioenergetics ,glutamine metabolism ,metabolic reprogramming ,cancer metabolism targeting ,Biology (General) ,QH301-705.5 ,Chemistry ,QD1-999 - Abstract
Mitochondrial dysfunction plays a significant role in the metabolic flexibility of cancer cells. This study aimed to investigate the metabolic alterations due to Coenzyme Q depletion in MCF-7 cells. Method: The Coenzyme Q depletion was induced by competitively inhibiting with 4-nitrobenzoate the coq2 enzyme, which catalyzes one of the final reactions in the biosynthetic pathway of CoQ. The bioenergetic and metabolic characteristics of control and coenzyme Q depleted cells were investigated using polarographic and spectroscopic assays. The effect of CoQ depletion on cell growth was analyzed in different metabolic conditions. Results: we showed that cancer cells could cope from energetic and oxidative stress due to mitochondrial dysfunction by reshaping their metabolism. In CoQ depleted cells, the glycolysis was upregulated together with increased glucose consumption, overexpression of GLUT1 and GLUT3, as well as activation of pyruvate kinase (PK). Moreover, the lactate secretion rate was reduced, suggesting that the pyruvate flux was redirected, toward anabolic pathways. Finally, we found a different expression pattern in enzymes involved in glutamine metabolism, and TCA cycle in CoQ depleted cells in comparison to controls. Conclusion: This work elucidated the metabolic alterations in CoQ-depleted cells and provided an insightful understanding of cancer metabolism targeting.
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- 2020
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18. Photoredox Propargylation of Aldehydes Catalytic in Titanium
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Andrea Gualandi, Marco Di Matteo, Giacomo Bergamini, Simone Potenti, Andrea Fermi, Pier Giorgio Cozzi, Francesco Calogero, Calogero, Francesco, Gualandi, Andrea, Matteo, Marco Di, Potenti, Simone, Fermi, Andrea, Bergamini, Giacomo, and Cozzi, Pier Giorgio
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010405 organic chemistry ,Organic Chemistry ,chemistry.chemical_element ,Note ,010402 general chemistry ,aldehydes, Photoredox, catalysis ,01 natural sciences ,0104 chemical sciences ,Catalysis ,chemistry.chemical_compound ,chemistry ,Propargyl ,Organic dye ,Organic chemistry ,Propargyl bromide ,Stoichiometry ,Titanium - Abstract
A practical and effective photoredox propargylation of aldehydes promoted by 10 mol % of [Cp2TiCl2] is presented. No stoichiometric metals or scavengers are used for the process. A catalytic amount of the cheap and simply prepared organic dye 3DPAFIPN is used as the reductant for titanium. The reaction displayed a broad scope, and no traces of allenyl isomers were detected for simple propargyl bromide, whereas mixtures of propargyl and allenyl isomers were observed for substituted propargyl bromides.
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- 2021
19. Sustainable Drug Discovery of Multi-Target-Directed Ligands for Alzheimer’s Disease
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Florian Nachon, Ondrej Soukup, Luciana de Camargo Nascente, Fabien Chantegreil, Michele Rossi, Marco Malaguti, Maria Laura Bolognesi, Sarah de Melo Viana Teixeira, Michela Freschi, Silvana Hrelia, Manuela Bartolini, Alessandra Salerno, Luiz Antonio Soares Romeiro, Christian Bergamini, Cristina Angeloni, Lukas Prchal, and Rossi M, Freschi M, de Camargo Nascente L, Salerno A, de Melo Viana Teixeira S, Nachon F, Chantegreil F, Soukup O, Prchal L, Malaguti M, Bergamini C, Bartolini M, Angeloni C, Hrelia S, Soares Romeiro LA, Bolognesi ML.
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Lipopolysaccharides ,Cell Survival ,Molecular Dynamics Simulation ,Pharmacology ,Ligands ,01 natural sciences ,Neuroprotection ,Article ,Cell Line ,ACETYLCHOLINESTERASE ,NEUROINFLAMMATION ,BUTYRYLCHOLINESTERASE ,MICROGLIA ,Structure-Activity Relationship ,03 medical and health sciences ,chemistry.chemical_compound ,Multi target ,Alzheimer Disease ,Catalytic Domain ,Drug Discovery ,medicine ,Humans ,Nuts ,Anacardium ,CRYSTAL-STRUCTURE ,Neuroinflammation ,Butyrylcholinesterase ,030304 developmental biology ,chemistry.chemical_classification ,0303 health sciences ,Binding Sites ,Plant Extracts ,Chemistry ,Drug discovery ,Acetylcholinesterase ,0104 chemical sciences ,010404 medicinal & biomolecular chemistry ,Neuroprotective Agents ,SUSTAINABLE DRUG DISCOVERY ,Enzyme ,ALZHEIMER'S DISEASE ,Drug Design ,Tacrine ,Cytokines ,Molecular Medicine ,TACRINE HYBRIDS ,medicine.drug - Abstract
The multifactorial nature of Alzheimer's disease (AD) is a reason for the lack of effective drugs as well as a basis for the development of "multi-target-directed ligands" (MTDLs). As cases increase in developing countries, there is a need of new drugs that are not only effective but also accessible. With this motivation, we report the first sustainable MTDLs, derived from cashew nutshell liquid (CNSL), an inexpensive food waste with anti-inflammatory properties. We applied a framework combination of functionalized CNSL components and well-established acetylcholinesterase (AChE)/butyrylcholinesterase (BChE) tacrine templates. MTDLs were selected based on hepatic, neuronal, and microglial cell toxicity. Enzymatic studies disclosed potent and selective AChE/BChE inhibitors (5, 6, and 12), with subnanomolar activities. The X-ray crystal structure of 5 complexed with BChE allowed rationalizing the observed activity (0.0352 nM). Investigation in BV-2 microglial cells revealed antineuroinflammatory and neuroprotective activities for 5 and 6 (already at 0.01 mu M), confirming the design rationale.
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- 2021
20. Abiraterone Acetate in Patients With Castration-Resistant, Androgen Receptor–Expressing Salivary Gland Cancer: A Phase II Trial
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Pasquale Quattrone, Laura D. Locati, Salvatore Alfieri, Luigi Mariani, Paolo Bossi, Lisa Licitra, Cristiana Bergamini, Francesca Platini, Carlo Resteghini, Elena Colombo, Giuseppina Calareso, Iolanda Capone, and Stefano Cavalieri
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Adult ,Male ,Cancer Research ,medicine.drug_class ,Abiraterone Acetate ,Antineoplastic Agents ,Castration resistant ,chemistry.chemical_compound ,medicine ,Humans ,In patient ,Aged ,Salivary gland ,business.industry ,Abiraterone acetate ,Middle Aged ,Salivary Gland Neoplasms ,Androgen ,medicine.disease ,Androgen receptor ,medicine.anatomical_structure ,Oncology ,chemistry ,Salivary gland cancer ,Cancer research ,Female ,business - Abstract
PURPOSE The activity of androgen-deprivation therapy (ADT) in androgen receptor–positive (AR+) salivary gland carcinomas (SGCs) has been established in the past few years. Second-line treatment in castration-resistant patients is still unknown. We investigated the activity of abiraterone acetate as second-line treatment in ADT-resistant, AR+ patients with SGC. METHODS This was a single-institution phase II trial. A two-stage Simon's design was applied. The primary end point was confirmed objective response rate. Secondary end points were disease control rate, safety, progression-free survival, and overall survival. Patients were eligible when the following criteria were met: histologic diagnosis of AR-overexpressing SGC, measurable disease according to RECIST 1.1, clinical and/or radiologic progression on ADT, suppressed serum testosterone, and no limits for the number of previous chemotherapy lines. All patients received abiraterone 1 g daily plus prednisone 10 mg and luteinizing hormone-releasing hormone agonist until progression or unacceptable toxicities. RESULTS From 2015 to 2019, 24 AR+ patients with SGC (23 men; median age 65.8 years) were treated within the study. The overall response rate was 21% (5 partial responses), with a disease control rate of 62.5%. The median duration of response was 5.82 months. Median progression-free survival was 3.65 months (95% CI, 1.94 to 5.89), and median overall survival was 22.47 months (95% CI, 6.74 to not reached). Objective response to previous ADT did not correlate with the activity of abiraterone. Adverse events (AEs) were recorded in 22 cases (92%) with grade 3 AEs in six patients (25%): fatigue (two), flushing (one), supraventricular tachycardia (one), and two non–drug-related AEs. No drug-related grade 4 or 5 AEs were recorded. CONCLUSION Abiraterone plus luteinizing hormone-releasing hormone agonist is active and safe as a second-line option in AR-expressing, castration-resistant SGC.
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- 2021
21. OPTICAL COHERENCE TOMOGRAPHY ANGIOGRAPHY FINDINGS IN A CASE OF CONGENITAL RETINAL MACROVESSEL WITH ANOMALOUS RETINAL ANASTOMOSIS ASSOCIATED WITH CONTRALATERAL MYELINATED NERVE FIBERS AND RETINAL VASCULAR ABNORMALITIES
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Fulvio Bergamini, Marco Antonio Pellegrini, Chiara Preziosa, Giovanni Staurenghi, Paola Ciasca, and Paolo Milani
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medicine.medical_specialty ,genetic structures ,Myelinated nerve fiber ,Anastomosis ,Fundus (eye) ,01 natural sciences ,03 medical and health sciences ,chemistry.chemical_compound ,0302 clinical medicine ,Optical coherence tomography ,Edema ,Ophthalmology ,Medicine ,0101 mathematics ,Macular edema ,medicine.diagnostic_test ,business.industry ,010102 general mathematics ,Retinal ,General Medicine ,medicine.disease ,Fluorescein angiography ,eye diseases ,chemistry ,030221 ophthalmology & optometry ,sense organs ,medicine.symptom ,business - Abstract
Purpose To describe a case of congenital retinal macrovessel complicated by cystoid macular edema associated with contralateral myelinated retinal nerve fibers and retinal vascular abnormalities studied with optical coherence tomography angiography (OCTA). Methods Case report. Results A healthy 25-year-old woman with decreased vision in her right eye was found to have a congenital retinal venous macrovessel in the macula associated with cystoid edema. In the contralateral amblyopic eye, the examination revealed a tuft of myelinated retinal nerve fibers along the superotemporal vascular arcade associated with superficial vascular abnormalities. A complete multi-imaging examination was obtained, including fundus color photography, fluorescein angiography, indocyanine green angiography, optical coherence tomography (OCT), and optical coherence tomography angiography. At 1-week follow-up, the optical coherence tomography displayed spontaneous resolution of the edema that remained stable at consecutive 1-month follow-up. Conclusion Congenital retinal macrovessels can be associated with other ocular developmental anomalies. Vascular complications can occur, leading to macular edema and retinal ischemia. Optical coherence tomography angiography can be useful for the diagnosis and follow-up of this condition.
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- 2021
22. Giant Shape-Persistent Tetrahedral Porphyrin System: Light-Induced Charge Separation
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Paola Ceroni, Barbara Ventura, Nicola Demitri, Nicola Armaroli, Marianna Marchini, Elisabetta Iengo, Giacomo Bergamini, Alessandra Luisa, Massimo Baroncini, Marchini M., Luisa A., Bergamini G., Armaroli N., Ventura B., Baroncini M., Demitri N., Iengo E., Ceroni P., Marchini, Marianna, Luisa, Alessandra, Bergamini, Giacomo, Armaroli, Nicola, Ventura, Barbara, Baroncini, Massimo, Demitri, Nicola, Iengo, Elisabetta, and Ceroni, Paola
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spectroscopy ,METHYL VIOLOGEN ,DENDRIMERS ,ENERGY ,MACROCYCLES ,ELECTRON ,PHOTOREDUCTION ,DYNAMICS ,HOST ,CORE ,Supramolecular chemistry ,chemistry.chemical_element ,010402 general chemistry ,Photochemistry ,porphyrins ,01 natural sciences ,Catalysis ,Photoinduced electron transfer ,chemistry.chemical_compound ,Electron transfer ,electron transfer ,ruthenium ,self-assembly ,Quenching (fluorescence) ,010405 organic chemistry ,Organic Chemistry ,General Chemistry ,Porphyrin ,0104 chemical sciences ,Ruthenium ,chemistry ,PHOTOPHYSICAL PROPERTIES ,Phosphorescence ,porphyrin ,Tetraphenylmethane - Abstract
Tetraphenylmethane appended with four pyridylpyridinium units works as a scaffold to self-assemble four ruthenium porphyrins in a tetrahedral shape-persistent giant architecture. The resulting supramolecular structure has been characterised in the solid state by X-ray single crystal analysis and in solution by various techniques. Multinuclear NMR spectroscopy confirms the 1 : 4 stoichiometry with the formation of a highly symmetric structure. The self-assembly process can be monitored by changes of the redox potentials, as well as by modifications in the visible absorption spectrum of the ruthenium porphyrin and by a complete quenching of both the bright fluorescence of the tetracationic scaffold and the weak phosphorescence of the ruthenium porphyrin. An ultrafast photoinduced electron transfer is responsible for this quenching process. The lifetime of the resulting charge separated state (800 ps) is about four times longer in the giant supramolecular structure compared to the model 1 : 1 complex formed by the ruthenium porphyrin and a single pyridylpyridinium unit. Electron delocalization over the tetrameric pyridinium structure is likely to be responsible for this effect.
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- 2021
23. Risk factors for postoperative morbidity following appendectomy in the elderly: a nationwide prospective cohort study
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Poillucci, Gaetano, Podda, Mauro, Pisanu, Adolfo, Mortola, Lorenzo, Dalla Caneva, Patrizia, Massa, Giulia, Costa, Gianluca, Savastano, Riccardo, Cillara, Nicola, Collaborative Study Group endorsed by SICUT ACOI SICG SICE and Italian Chapter of WSES, Agresta, F, Alemanno, G, Anania, G, Antropoli, M, Argenio, G, Atzeni, J, Avenia, N, Azzinnaro, A, Baldazzi, G, Balducci, G, Barbera, G, Bellanova, G, Bergamini, C, Bersigotti, L, Bianchi, Pp, Bombardini, C, Borzellino, G, Bozzo, S, Brachini, G, Buonanno, Gm, Canini, T, Cardella, S, Carrara, G, Cassini, D, Castriconi, M, Ceccarelli, G, Celi, D, Ceresoli, M, Chiarugi, M, Cillara, N, Cimino, F, Cobuccio, L, Cocorullo, G, Colangelo, E, Costa, G, Crucitti, A, Dalla Caneva, P, De Luca, M, De Manzoni Garberini, A, De Nisco, C, De Prizio, M, De Sol, A, Dibella, A, Falcioni, T, Falco, N, Farina, C, Finotti, E, Fontana, T, Francioni, G, Fransvea, P, Frezza, B, Garulli, G, Genna, M, Giannessi, S, Gioffrè, A, Giordano, A, Gozzo, D, Grimaldi, S, Gulotta, G, Iacopini, V, Iarussi, T, Laterza, E, Leonardi, A, Lepre, L, Lorenzon, L, Luridiana, G, Malagnino, A, Mar, G, Marini, P, Marzaioli, R, Massa, G, Mecarelli, V, Mingoli, A, Nigri, G, Occhionorelli, S, Paderno, N, Palini, Gm, Paradies, D, Paroli, M, Perrone, F, Petruzzelli, L, Pezzolla, A, Piazza, D, Piazza, V, Piccoli, M, Pisanu, A, Podda, M, Poillucci, G, Porfidia, R, Rossi, G, Ruscelli, P, Spagnoli, A, Sulis, R, Tartaglia, D, Tranà, C, Travaglino, A, Tomaiuolo, P, Valeri, A, Vasquez, G, Zago, M, Zanoni, E., Poillucci G., Podda M., Pisanu A., Mortola L., Dalla Caneva P., Massa G., Costa G., Savastano R., Cillara N., Agresta F., Alemanno G., Anania G., Antropoli M., Argenio G., Atzeni J., Avenia N., Azzinnaro A., Baldazzi G., Balducci G., Barbera G., Bellanova G., Bergamini C., Bersigotti L., Bianchi P.P., Bombardini C., Borzellino G., Bozzo S., Brachini G., Buonanno G.M., Canini T., Cardella S., Carrara G., Cassini D., Castriconi M., Ceccarelli G., Celi D., Ceresoli M., Chiarugi M., Cimino F., Cobuccio L., Cocorullo G., Colangelo E., Crucitti A., De Luca M., De Manzoni Garberini A., De Nisco C., De Prizio M., De Sol A., Dibella A., Falcioni T., Falco N., Farina C., Finotti E., Fontana T., Francioni G., Fransvea P., Frezza B., Garulli G., Genna M., Giannessi S., Gioffre A., Giordano A., Gozzo D., Grimaldi S., Gulotta G., Iacopini V., Iarussi T., Laterza E., Leonardi A., Lepre L., Lorenzon L., Luridiana G., Malagnino A., Mar G., Marini P., Marzaioli R., Mecarelli V., Mingoli A., Nigri G., Occhionorelli S., Paderno N., Palini G.M., Paradies D., Paroli M., Perrone F., Petruzzelli L., Pezzolla A., Piazza D., Piazza V., Piccoli M., Porfidia R., Rossi G., Ruscelli P., Spagnoli A., Sulis R., Tartaglia D., Trana C., Travaglino A., Tomaiuolo P., Valeri A., Vasquez G., Zago M., Zanoni E., Poillucci, G, Podda, M, Pisanu, A, Mortola, L, Dalla Caneva, P, Massa, G, Costa, G, Savastano, R, Cillara, N, Agresta, F, Alemanno, G, Anania, G, Antropoli, M, Argenio, G, Atzeni, J, Avenia, N, Azzinnaro, A, Baldazzi, G, Balducci, G, Barbera, G, Bellanova, G, Bergamini, C, Bersigotti, L, Bianchi, P, Bombardini, C, Borzellino, G, Bozzo, S, Brachini, G, Buonanno, G, Canini, T, Cardella, S, Carrara, G, Cassini, D, Castriconi, M, Ceccarelli, G, Celi, D, Ceresoli, M, Chiarugi, M, Cimino, F, Cobuccio, L, Cocorullo, G, Colangelo, E, Crucitti, A, De Luca, M, De Manzoni Garberini, A, De Nisco, C, De Prizio, M, De Sol, A, Dibella, A, Falcioni, T, Falco, N, Farina, C, Finotti, E, Fontana, T, Francioni, G, Fransvea, P, Frezza, B, Garulli, G, Genna, M, Giannessi, S, Gioffre, A, Giordano, A, Gozzo, D, Grimaldi, S, Gulotta, G, Iacopini, V, Iarussi, T, Laterza, E, Leonardi, A, Lepre, L, Lorenzon, L, Luridiana, G, Malagnino, A, Mar, G, Marini, P, Marzaioli, R, Mecarelli, V, Mingoli, A, Nigri, G, Occhionorelli, S, Paderno, N, Palini, G, Paradies, D, Paroli, M, Perrone, F, Petruzzelli, L, Pezzolla, A, Piazza, D, Piazza, V, Piccoli, M, Porfidia, R, Rossi, G, Ruscelli, P, Spagnoli, A, Sulis, R, Tartaglia, D, Trana, C, Travaglino, A, Tomaiuolo, P, Valeri, A, Vasquez, G, Zago, M, and Zanoni, E
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medicine.medical_specialty ,Multivariate analysis ,Sports medicine ,Settore MED/18 - CHIRURGIA GENERALE ,Critical Care and Intensive Care Medicine ,Acute appendicitis ,Appendectomy ,Elderly ,Frail patients ,Postoperative complications ,NO ,03 medical and health sciences ,chemistry.chemical_compound ,0302 clinical medicine ,Risk Factors ,Acute appendicitis, Appendectomy, Elderly, Frail patients, Postoperative complications ,Internal medicine ,medicine ,Humans ,Frail patient ,Orthopedics and Sports Medicine ,In patient ,Prospective Studies ,Prospective cohort study ,Aged ,Retrospective Studies ,030222 orthopedics ,Creatinine ,business.industry ,Postoperative complication ,030208 emergency & critical care medicine ,Perioperative ,Length of Stay ,Appendicitis ,chemistry ,Emergency Medicine ,Laparoscopy ,Surgery ,Acute appendiciti ,Morbidity ,Risk assessment ,business - Abstract
Background: A limited number of studies investigating perioperative risk factors associated with emergency appendectomy in elderly patients have been published to date. Whether older age may be associated with poorer outcomes following appendectomy is still a matter of debate. The primary aim of this study was to determine the predictors of postoperative morbidity following appendectomy in patients aged ≥ 65years. Methods: Data regarding all elderly patients who underwent emergency appendectomy from January 2017 to June 2018 admitted 36 Italian surgical departments were prospectively collected and analyzed. Baseline demographics and perioperative variables were evaluated. Uni- and multivariate analyses adjusted for differences between groups were carried out to determine possible predictors of adverse outcomes after appendectomy. Results: Between January 2017 and June 2018, 135 patients aged ≥ 65years with a diagnosis of AA met the study inclusion criteria. Twenty-six patients (19.3%) were diagnosed with some type of postoperative complication. Decreasing the preoperative hemoglobin level showed a statistically significant association with postoperative complications (OR 0.77, CI 0.61–0.97, P = 0.03). Preoperative creatinine level (P = 0.02, OR 2.04, CI 1.12–3.72), and open appendectomy (P = 0.03, OR 2.67, CI 1.11–6.38) were significantly associated with postoperative morbidity. After adjustment, the only independent predictor of postoperative morbidity was preoperative creatinine level (P = 0.04, OR 2.01, CI 1.05–3.89). Conclusions: In elderly patients with AA, perioperative risk assessment in the emergency setting must be as accurate as possible to identify modifiable risk factors that can be addressed before surgery, such as preoperative hemoglobin and creatinine levels.
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- 2019
24. Highly Emissive Water‐Soluble Polysulfurated Pyrene‐Based Chromophores as Dual Mode Sensors of Metal Ions
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Paola Ceroni, Marco Villa, Myriam Roy, Giacomo Bergamini, Marc Gingras, Villa M., Roy M., Bergamini G., Ceroni P., and Gingras M.
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chemistry.chemical_classification ,Aqueous solution ,010405 organic chemistry ,Metal ions in aqueous solution ,Carboxylic acid ,Supramolecular chemistry ,pyrene ,Ethylenediaminetetraacetic acid ,General Chemistry ,Chromophore ,010402 general chemistry ,Photochemistry ,01 natural sciences ,metal ion ,supramolecular chemistry ,0104 chemical sciences ,chemistry.chemical_compound ,Deprotonation ,chemistry ,sensor ,luminescence ,Pyrene - Abstract
Pyrene-based materials have gained considerable attention as stimuli-responsive chemical sensors. We designed a polysulfurated arene system based on a tetra(phenylthio)pyrene core decorated with four carboxylic acid units. Three different regioisomers, ortho, meta and para were studied in organic and aqueous solution. These systems are soluble in water at pH≥8 due to the deprotonation of carboxylic acids. The addition of metal ions cannot only quench the fluorescence of the central pyrene core, but also control the formation of three-dimensional nanoscopic objects in a dual mode function. Several divalent metal ions were tested and compared. Addition of ethylenediaminetetraacetic acid (EDTA) disassembles the non-emissive supramolecular system and restores the initial fluorescence of the pyrene core.
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- 2020
25. BID and the α-bisabolol-triggered cell death program: converging on mitochondria and lysosomes
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Erika Lorenzetto, Irene Liparulo, Fabrizio Vinante, Isacco Ferrarini, Antonella Rigo, Cinzia Sissa, Elisabetta Cavalieri, Christian Bergamini, Elena Darra, Rigo, Antonella, Ferrarini, Isacco, Lorenzetto, Erika, Darra, Elena, Liparulo, Irene, Bergamini, Christian, Sissa, Cinzia, Cavalieri, Elisabetta, and Vinante, Fabrizio
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0301 basic medicine ,Cancer Research ,Programmed cell death ,Cancer therapy ,α-bisabolol, BID, apoptosis, autophagy ,Immunology ,Apoptosis ,G(M1) Ganglioside ,Mitochondrion ,Article ,Cathepsin B ,Cell Line ,BID ,03 medical and health sciences ,Cellular and Molecular Neuroscience ,α-bisabolol ,Membrane Microdomains ,0302 clinical medicine ,Autophagy ,Humans ,lcsh:QH573-671 ,Lipid raft ,Membrane Potential, Mitochondrial ,Chemistry ,lcsh:Cytology ,Cell Cycle ,Membrane Proteins ,Cell Biology ,Carbocyanines ,Mitochondria ,Cell biology ,Monocyclic Sesquiterpenes ,Protein Transport ,Cytosol ,030104 developmental biology ,Preclinical research ,Gene Knockdown Techniques ,030220 oncology & carcinogenesis ,Neoplastic cell ,Benzimidazoles ,Lysosomes ,bisabolol, mitochondria, bid, lysosomes, cancer ,BH3 Interacting Domain Death Agonist Protein - Abstract
α-Bisabolol (BSB) is a plant-derived sesquiterpene alcohol able to trigger regulated cell death in transformed cells, while deprived of the general toxicity in several mouse models. Here, we investigated the involvement of lysosomal and mitochondrial compartments in the cytotoxic effects of BSB, with a specific focus on the BH3-only activator protein BID. We found that BSB particularly accumulated in cancer cell lines, displaying a higher amount of lipid rafts as compared to normal blood cells. By means of western blotting and microscopy techniques, we documented rapid BSB-induced BID translocation to lysosomes and mitochondria, both of them becoming dysfunctional. Lysosomal membranes were permeabilized, thus blocking the cytoprotective autophagic flux and provoking cathepsin B leakage into the cytosol. Multiple flow cytometry-based experiments demonstrated the loss of mitochondrial membrane potential due to pore formation across the lipid bilayer. These parallel events converged on neoplastic cell death, an outcome significantly prevented by BID knockdown. Therefore, BSB promoted BID redistribution to the cell death executioner organelles, which in turn activated anti-autophagic and proapoptotic mechanisms. This is an example of how xenohormesis can be exploited to modulate basic cellular programs in cancer.
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- 2019
26. Evaluation of Titanate Nanotubes (TiNTs) as a Modifier for the Determination of Lead (II) by Differential Pulse Adsorptive Stripping Voltammetry (DPAdSV)
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Luiz H. Marcolino-Junior, Amanda Maciel, Eduardo H. Bindewald, and Márcio F. Bergamini
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Titanate nanotubes ,Chemistry ,Biochemistry (medical) ,Clinical Biochemistry ,Adsorptive stripping voltammetry ,Inorganic chemistry ,Electrochemistry ,Biochemistry ,Spectroscopy ,Analytical Chemistry ,Electrochemical gas sensor - Abstract
This work reports the synthesis and characterization of titanate nanotubes (TiNTs) and their use for the construction of an electrochemical sensor for lead (II) determination in water samples. TiNTs were prepared from TiO2 by a hydrothermal route at 150 ��C for 24 h in alkaline conditions. X-ray diffraction (XRD) measurements show peak enlargement and the presence of trititanate with different spaces between layers confirming the production of TiNTs of nanometric dimensions. In addition, scanning electron microscopy (SEM) and transmission electron microscopy (TEM) images demonstrated the presence of dispersed, homogenous, and nanometric tubular structure. TiNTs were used for the modification of carbon paste electrodes and evaluated for determination of lead (II) by differential pulse adsorptive stripping voltammetry (DPAdSV). Using the optimized conditions, the developed method provided a linear dynamic range (LDR) from 0.77 to 7.71 ��M and limits of detection and quantification of 0.23 and 0.77 ��M, respectively. Studies of addition and recovery in spiked water sample were performed, obtaining a recovery of 104.6%. A repeatability study resulted in a relative standard deviation of 11% for consecutive measurements. Using the same electrode, a reproducibility study provided a relative standard deviation of 3.9%.
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- 2021
27. Bleeding complications in patients with squamous cell carcinoma of the head and neck
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Jing Xie, Muzammil Ali, Kevin J. Harrington, Robert L. Ferris, Amanda Psyrri, Lisa Licitra, William C. Holmes, Stefano Cavalieri, Cristiana Bergamini, and Gabriella Mariani
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Oncology ,Vascular Endothelial Growth Factor A ,medicine.medical_specialty ,Clinical Review ,Immune checkpoint inhibitors ,medicine.medical_treatment ,head and neck squamous cell carcinoma ,Clinical Reviews ,immune checkpoint inhibitors ,03 medical and health sciences ,chemistry.chemical_compound ,0302 clinical medicine ,Internal medicine ,Epidemiology ,Medicine ,Humans ,030212 general & internal medicine ,Epidermal growth factor receptor ,antiangiogenic drugs ,Head and neck ,Chemotherapy ,biology ,business.industry ,Squamous Cell Carcinoma of Head and Neck ,medicine.disease ,Head and neck squamous-cell carcinoma ,Clinical trial ,Vascular endothelial growth factor ,stomatognathic diseases ,Otorhinolaryngology ,chemistry ,Head and Neck Neoplasms ,030220 oncology & carcinogenesis ,biology.protein ,Carcinoma, Squamous Cell ,molecular targeted therapies ,hemorrhage ,Neoplasm Recurrence, Local ,business - Abstract
Hemorrhage in recurrent and/or metastatic (R/M) head and neck squamous cell carcinoma (HNSCC) may be attributed to chemotherapy and local tumor irradiation. Evidence of the relationship between hemorrhage in R/M HNSCC and targeted therapies, including epidermal growth factor receptor (EGFR) and vascular endothelial growth factor (VEGF) inhibitors, or immune checkpoint inhibitors, is limited. We aimed to identify epidemiological and clinical data related to the occurrence of hemorrhage in R/M HNSCC and to explore its relationship with various therapies. We describe information obtained from literature searches as well as data extracted from a commercial database and a database from the author's institution (Istituto Nazionale dei Tumori of Milan). Evidence suggests that most bleeding events in R/M HNSCC are minor. Clinical trial safety data do not identify a causal association between hemorrhage and anti‐EGFR agents or immune checkpoint inhibitors. In contrast, anti‐VEGF agents are associated with increased, and often severe/fatal, hemorrhagic complications.
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- 2021
28. NCX 1741, a Novel Nitric Oxide-Donating Phosphodiesterase-5 Inhibitor, Exerts Rapid and Long-Lasting Intraocular Pressure-Lowering in Cynomolgus Monkeys
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Jean Michel Bukowski, Laura Lucarini, Tomas Navratil, Stefania Brambilla, Francesco Impagnatiello, Michael V.W. Bergamini, Corinna Galli, Carol B. Toris, Elena Bastia, and Nicoletta Almirante
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Long lasting ,Intraocular pressure ,genetic structures ,medicine.drug_mechanism_of_action ,Avanafil ,Pharmacology ,Dinoprost ,Nitric Oxide ,Nitric oxide ,Aqueous Humor ,Tonometry, Ocular ,chemistry.chemical_compound ,Travoprost ,Tandem Mass Spectrometry ,medicine ,Animals ,Nitric Oxide Donors ,Pharmacology (medical) ,Antihypertensive Agents ,Intraocular Pressure ,Phosphodiesterase ,Phosphodiesterase 5 Inhibitors ,Macaca fascicularis ,Ophthalmology ,Pyrimidines ,chemistry ,Models, Animal ,Anti-Infective Agents, Local ,Female ,Ocular Hypertension ,Rabbits ,Benzalkonium Compounds ,Phosphodiesterase 5 inhibitor ,Derivative (chemistry) ,Chromatography, Liquid ,Follow-Up Studies ,medicine.drug - Abstract
Purpose: We studied the IOP-lowering effects of NCX 1741, a novel nitric oxide (NO)-donating derivative of the phosphodiesterase type-5 inhibitor, avanafil, in Cynomolgus monkey with laser-induced ...
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- 2021
29. Nafion®-Containing Solid Lipid Nanoparticles as a Tool for Anticancer Pt Delivery: Preliminary Studies
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Maddalena Sguizzato, Elisabetta Esposito, Marcus Drechsler, Eleonora Gallerani, Riccardo Gavioli, Paolo Mariani, Federica Carducci, Rita Cortesi, and Paola Bergamini
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Chemistry ,QD1-999 - Abstract
Preliminary studies of nanoparticles based on the perfluorosulfonic acid resin Nafion have been carried out with the aim of establishing an ionic connection with the protonable phosphine 1,3,5-triaza-7-phosphaadamantane (PTA), suitable for the coordination of platinum. Nafion-containing nanoparticles (NAF) were produced by homogenization followed by ultrasonication method. After production, NAF were characterized in terms of size, morphology, and in vitro cytotoxicity. The PCS studies showed that the Z-average mean diameter was around 250 nm. Moreover, the polydispersity index showed a monodimensional distribution of nanoparticles. Cryo-TEM analysis showed a uniform and homogenous population of particles, characterized by the presence of both ovoidal and needle-like structures. To evaluate the in vitro cytotoxicity, NAF were tested on human cancer cell lines K562 and A2780. No cytotoxic effect was found on both cell lines. By 13P-NMR measures, it is here proved that the strongly acidic sulfonic groups of Nafion-containing nanoparticles (NAF) can act as protonating agents for PTA. The protonation occurs selectively at nitrogen; hence protonated PTA maintains its ability to coordinate platinum via its phosphorus atom.
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- 2017
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30. Late toxicities burden in patients with radioiodine-refractory differentiated thyroid cancer treated with lenvatinib
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Lisa Licitra, Francesca Platini, E Seregni, Stefano Cavalieri, Laura D. Locati, Salvatore Alfieri, Carlo Resteghini, Biagio Paolini, L Mazzeo, Cristiana Bergamini, Elena Colombo, and A. Bottiglieri
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medicine.medical_specialty ,Side effect ,Endocrinology, Diabetes and Metabolism ,Antineoplastic Agents ,030209 endocrinology & metabolism ,Iodine Radioisotopes ,03 medical and health sciences ,chemistry.chemical_compound ,0302 clinical medicine ,Endocrinology ,Refractory ,Internal medicine ,Diabetes mellitus ,medicine ,Humans ,Thyroid Neoplasms ,Adverse effect ,Protein Kinase Inhibitors ,Thyroid cancer ,Survival analysis ,Retrospective Studies ,business.industry ,Phenylurea Compounds ,Incidence (epidemiology) ,medicine.disease ,chemistry ,030220 oncology & carcinogenesis ,Quinolines ,Female ,business ,Lenvatinib - Abstract
Radioactive-iodine (RAI)-resistant differentiated thyroid cancer (DTC) patients benefit from multi-kinase inhibitors (MKIs), such as lenvatinib. Incidence of treatment-related (TR) late toxicities has been not yet described. From January 2015 to June 2019 we retrospectively reviewed clinical records of patients with RAI-resistant DTC treated with lenvatinib at Istituto Nazionale dei Tumori (Milan, Italy). New side effect of any grade, appeared after 12 months of lenvatinib, was defined as late adverse event (AE). Descriptive analyses were performed. Survival curves were estimated with Kaplan–Meier method and compared with log-rank test. Thirty-seven patients were included, 65% had ≥65 years and 68% were female. Thirty patients received lenvatinib for >12 months. Lenvatinib was started at ≤20 mg/daily in 59% of patients, 64% were ≥65 years. The frequency of late AEs was 80% and cardiovascular toxicity was the most common (57%). There was no difference in the incidence of late AEs between younger/older population (77% and 82%, respectively). Median lenvatinib treatment duration (TD) was 39.96 months (95% CI 21.64-NR): 39.96 months for patients
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- 2021
31. amTCO, a newtrans-cyclooctene derivative to study drug-target interactions in cells
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Ana Kojic, Anna Rutkowska, Blandine S J McKay, Carsten Schultz, Douglas W. Thomson, Giovanna Bergamini, Marcel Mulbaier, Lee Edwards, and Cécile Echalier
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010402 general chemistry ,01 natural sciences ,Catalysis ,Phthalimide ,Cyclooctanes ,Structure-Activity Relationship ,chemistry.chemical_compound ,Drug Delivery Systems ,Cyclooctene ,Drug Discovery ,Materials Chemistry ,Humans ,Indoleamine-Pyrrole 2,3,-Dioxygenase ,Moiety ,Protecting group ,Molecular Structure ,010405 organic chemistry ,Chemistry ,Metals and Alloys ,General Chemistry ,Combinatorial chemistry ,Small molecule ,0104 chemical sciences ,Surfaces, Coatings and Films ,Electronic, Optical and Magnetic Materials ,Molecular Docking Simulation ,Ceramics and Composites ,Click chemistry ,Click Chemistry ,Isomerization ,Derivative (chemistry) ,HeLa Cells - Abstract
Click chemistry probes have improved the study of drug interactions in live cells and relevant disease models. Proper design of the probes, including the choice of the click moiety coupled to the drug, is crucial to ensure good performance and broad application. A new trans-cyclooctene derivative, amTCO, was synthesised via a novel route using a phthalimide protecting group as a built-in photosensitiser for the cyclooctene isomerization. amTCO improved the physical chemical properties of click chemistry probes compared to standard TCO moieties. An amTCO probe targeting indoleamine 2,3-dioxygenase (IDO1) was a superior tool for visualizing IDO1 and measuring the binding affinities of small molecule inhibitors to IDO1 in cells.
- Published
- 2021
32. CsPbBr3 for photoelectrochemical cells
- Author
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Linda Bergamini, Alessandra Sanson, Nicola Sangiorgi, and Angela Gondolini
- Subjects
Photocurrent ,Materials science ,Aqueous solution ,Renewable Energy, Sustainability and the Environment ,020209 energy ,02 engineering and technology ,Photoelectrochemical cell ,021001 nanoscience & nanotechnology ,Electrochemistry ,Rhodamine ,chemistry.chemical_compound ,Chemical engineering ,chemistry ,0202 electrical engineering, electronic engineering, information engineering ,Photocatalysis ,Energy transformation ,General Materials Science ,0210 nano-technology ,Perovskite (structure) - Abstract
Due to the superior optical and electronic properties, all-inorganic perovskite materials have been widely used in optoelectronic device and energy conversion systems such as solar cells. However, photoelectrochemical devices with all-inorganic perovskite as photo-active material used in aqueous solution have been received not many attentions. In this work, the use of CsPbBr3 in photoelectrochemical system in direct contact with water is studied for the first time, considering also the post-mortem properties. Sub-micronic crystals of CsPbBr3 were synthetized by a conventional low temperature synthesis and the obtained materials were used to produce a photo-electrode for photo-electrochemical cell (PEC). The material was characterized and then tested to assess its electrochemical and photocatalysis properties. The particles showed high photocatalytic activity (more than 50% of Rhodamine degradation after 80 min) and promising photocurrent values of 11.7 µA cm−2 and 2.25 μA cm−2 in reduction and oxidation conditions respectively. The synthesis conditions assured CsPbBr3 good stability in water up to 60 min. After this time, system passes from pure CsPbBr3 to a mixture of CsPbBr3 and Cs4PbBr6 still retaining part of its photoelectrochemical activity. The results showed that CsPbBr3 has good potentiality as photo-electrode material in water-based photoelectrochemical cell for the conversion of CO2 into fuels or chemicals.
- Published
- 2020
33. 3D-printed electrode as a new platform for electrochemical immunosensors for virus detection
- Author
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Lucas H. Budni, Bruno C. Janegitz, Gustavo Martins, Luiz H. Marcolino-Junior, Márcio F. Bergamini, and Jeferson L. Gogola
- Subjects
Thermogravimetric analysis ,Orthohantavirus ,Hantavirus Infections ,Hantavirus araucaria ,02 engineering and technology ,Immunosensor ,Antibodies, Viral ,01 natural sciences ,Biochemistry ,Article ,Analytical Chemistry ,Protein filament ,chemistry.chemical_compound ,Polylactic acid ,Soot ,3D conductive filament ,Limit of Detection ,Environmental Chemistry ,Humans ,Electrodes ,Spectroscopy ,Detection limit ,chemistry.chemical_classification ,Virus detection ,Immunoassay ,SARS-CoV-2 ,Biomolecule ,010401 analytical chemistry ,COVID-19 ,Carbon black ,Nucleocapsid Proteins ,021001 nanoscience & nanotechnology ,0104 chemical sciences ,Dielectric spectroscopy ,chemistry ,Chemical engineering ,Dielectric Spectroscopy ,Printing, Three-Dimensional ,Cyclic voltammetry ,0210 nano-technology ,Antibodies, Immobilized - Abstract
Simple, low-cost, and sensitive new platforms for electrochemical immunosensors for virus detection have been attracted attention due to the recent pandemic caused by a new type of coronavirus (SARS-CoV-2). In the present work, we report for the first time the construction of an immunosensor using a commercial 3D conductive filament of carbon black and polylactic acid (PLA) to detect Hantavirus Araucaria nucleoprotein (Np) as a proof-of-concept. The recognition biomolecule was anchored directly at the filament surface by using N-(3-Dimethylaminopropyl)-N′-ethylcarbodiimide hydrochloride and N-Hydroxysuccinimide (EDC/NHS). Conductive and non-conductive composites of PLA were characterized using thermal gravimetric analysis (TGA), revealing around 30% w/w of carbon in the filament. Morphological features of composites were obtained from SEM and TEM measurements. FTIR measurement revealed that crosslinking agents were covalently bonded at the filament surface. Electrochemical techniques such as cyclic voltammetry (CV) and electrochemical impedance spectroscopy (EIS) were used for the evaluation of each step involved in the construction of the proposed immunosensor. The results showed the potentiality of the device for the quantitative detection of Hantavirus Araucaria nucleoprotein (Np) from 30 μg mL−1 to 240 μg mL−1 with a limit of detection of 22 μg mL−1. Also, the proposed immunosensor was applied with success for virus detection in 100x diluted human serum samples. Therefore, the PLA conductive filament with carbon black is a simple and excellent platform for immunosensing, which offers naturally carboxylic groups able to anchor covalently biomolecules., Graphical abstract Image 1
- Published
- 2020
34. One- and two-photon absorption properties of quadrupolar thiophene-based dyes with acceptors of varying strengths
- Author
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Paola Ceroni, Sofia Canola, Fabrizia Negri, Francesca Di Maria, Giacomo Bergamini, Sergei A. Vinogradov, Angela Acocella, Marco Villa, Luca Ravotto, Lorenzo Mardegan, Mattia Zangoli, Canola S., Mardegan L., Bergamini G., Villa M., Acocella A., Zangoli M., Ravotto L., Vinogradov S.A., Di Maria F., Ceroni P., and Negri F.
- Subjects
Materials science ,oligothiophenes ,02 engineering and technology ,010402 general chemistry ,01 natural sciences ,Two-photon absorption ,Molecular physics ,Polarizable continuum model ,Article ,chemistry.chemical_compound ,two photon absorption, quantum chemical calculations ,Thiophene ,Intermediate state ,Physics::Chemical Physics ,Physical and Theoretical Chemistry ,Absorption (electromagnetic radiation) ,quadrupolar dyes ,Chromophore ,021001 nanoscience & nanotechnology ,Acceptor ,0104 chemical sciences ,chemistry ,One- and Two-photon Absorption ,Density functional theory ,0210 nano-technology ,acceptor strength - Abstract
The one-photon (1P) and two-photon (2P) absorption properties of three quadrupolar dyes, featuring thiophene as a donor and acceptors of varying strengths, are determined by a combination of experimental and computational methods employing the density functional theory (DFT). The emission shifts in different solvents are well reproduced by time-dependent DFT calculations with the linear response and state specific approaches in the framework of the polarizable continuum model. The calculations show that the energies of both 1P- and 2P-active states decrease with an increase of the strength of the acceptor. The 2P absorption cross-sections predicted by the response theory are accounted for by considering just one intermediate state (S1) in the sum-over-states formulation. For the chromophore featuring the stronger acceptor, the energetic positions of the 1P- and 2P-active states prevent the exploitation of the theoretically predicted very high 2P activity due to the competing 1P absorption into the S1 state.
- Published
- 2019
35. Diurnal Stability Of Peripapillary Vessel Density And Nerve Fiber Layer Thickness On Optical Coherence Tomography Angiography In Healthy, Ocular Hypertension And Glaucoma Eyes
- Author
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Luciana Carmassi, Ennio Bulone, Antonella Zambon, Elena Fratantonio, Lorenza Scotti, Sara Bochicchio, Lara Enrica Urbini, Giacomo Castegna, Fulvio Bergamini, Paolo Milani, Bochicchio, S, Milani, P, Urbini, L, Bulone, E, Carmassi, L, Fratantonio, E, Castegna, G, Scotti, L, Zambon, A, and Bergamini, F
- Subjects
medicine.medical_specialty ,genetic structures ,Nerve fiber layer ,Ocular hypertension ,Glaucoma ,optical coherence tomography angiography ,03 medical and health sciences ,chemistry.chemical_compound ,0302 clinical medicine ,Ophthalmology ,vessel density ,Medicine ,Original Research ,Morning ,business.industry ,Clinical Ophthalmology ,Retinal ,Optical coherence tomography angiography ,retinal fiber layer ,RE1-994 ,medicine.disease ,eye diseases ,Ganglion ,medicine.anatomical_structure ,Daily fluctuation ,chemistry ,030221 ophthalmology & optometry ,Optic nerve ,daily fluctuations ,sense organs ,business ,030217 neurology & neurosurgery - Abstract
Sara Bochicchio,1 Paolo Milani,1 Lara Enrica Urbini,1 Ennio Bulone,1 Luciana Carmassi,1 Elena Fratantonio,1 Giacomo Castegna,1 Lorenza Scotti,2 Antonella Zambon,2 Fulvio Bergamini1 1Ophthalmology Department, Istituto Auxologico Italiano, Milan, Italy; 2Statistics and Quantitative Methods Department, University of Milano-Bicocca, Milan, ItalyCorrespondence: Paolo MilaniIstituto Auxologico Italiano, Via Mercalli 30, Milan, ItalyTel +39 2 3385840232Fax +39 2 20422262Email dottpaolomilani@hotmail.comPurpose: By using optical coherence tomography angiography (OCTA), to evaluate diurnal changes of the following parameters: vessel density in the peripapillary area (PP-VD) and in the optic nerve head (ONH-VD); thickness of retinal nerve fibre layer (RNFL) and ganglion cell complex (GCC).Methods: All prospectively enrolled participants were imaged on the same day at 8 am and 7 pm by means of the XR Avanti device with AngioVue and AngioAnalytics software (Optovue Inc., Fremont, CA, USA). Only eyes with correct automatic segmentation and good-quality images (scan quality > 7/10) were included.Results: Forty-six eyes from 28 patients with glaucoma, 53 from 31 patients with ocular hypertension, and 62 from 38 controls were assessed. The mean measurements of all parameters investigated were significantly different in the morning and in the evening values in all three groups, with lower values in glaucomatous eyes (p0.1).Conclusion: Vessel density, GCC and RNFL were stable during daytime thus corroborating the clinical relevance of OCTA regardless the time of acquisition.Keywords: glaucoma, optical coherence tomography angiography, vessel density, daily fluctuations, retinal fiber layer
- Published
- 2019
36. Mercury isles in titanate nanotubes: a new strategy for using mercury electrodes in analytical application
- Author
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Edmílson Kleinert, Luiz H. Marcolino-Junior, Ediane Angelo, Eduardo H. Bindewald, and Márcio F. Bergamini
- Subjects
Detection limit ,010405 organic chemistry ,Open-circuit voltage ,Chemistry ,Analytical chemistry ,chemistry.chemical_element ,General Chemistry ,010402 general chemistry ,Electrochemistry ,01 natural sciences ,0104 chemical sciences ,Anode ,Mercury (element) ,Carbon paste electrode ,Adsorption ,Electrode - Abstract
This work reports for the first time the use of titanate nanotubes (TiNT) as adsorbent structure for Hg2+ ions and its use in construction of mercury-based electrodes for analytical applications. TiNTs with diameter of around 15 nm and length between 40 and 200 nm were obtained by hydrothermal alkaline synthesis and characterized by FTIR, DRX, and TEM. A carbon paste electrode modified with 15% (w/w) of TiNT was used for spontaneous mercury ions incorporation, by simply immerging in a Hg2+ solution for 120 s. “Mercury isles” were obtained by electrochemical reduction of adsorbed Hg2+ ions. Under the best optimized conditions, the proposed device was evaluated for the determination of Zn2+ ions in pharmaceutical samples. A linear relationship of anodic peak current and Zn2+ ions concentration was observed at a range of 4.0–20 µmol dm−3, with sensitivity of 0.54 μA dm3 μmol−1, limit of detection and limit of quantification of 1.2 µmol dm−3 and 4.0 µmol dm−3, respectively. Satisfactory agreement with a comparative method showed the useful application of the sensor, with the advantage of the Zn preconcentration step at open circuit potential condition. This strategy allows the use of several electrodes at the same time, which characterize this device as a feasible passive sampler.
- Published
- 2020
37. Carboplatin Use in Pregnancy for Stage IB3 Cervical Cancer: Case Report and Review of the Literature
- Author
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Alice Bergamini, Giorgia Mangili, Serena Girardelli, Massimo Candiani, Micaela Petrone, Emanuela Rabaiotti, and Luca Valsecchi
- Subjects
Adult ,Oncology ,medicine.medical_specialty ,medicine.medical_treatment ,Uterine Cervical Neoplasms ,Antineoplastic Agents ,Carboplatin ,03 medical and health sciences ,chemistry.chemical_compound ,0302 clinical medicine ,Ototoxicity ,Internal medicine ,Humans ,Medicine ,030212 general & internal medicine ,Young adult ,Stage (cooking) ,Neoplasm Staging ,Cisplatin ,Cervical cancer ,Chemotherapy ,Pregnancy ,business.industry ,medicine.disease ,chemistry ,030220 oncology & carcinogenesis ,Pediatrics, Perinatology and Child Health ,Female ,business ,medicine.drug - Abstract
Locally advanced stage cervical cancer diagnosed during pregnancy is a clinical challenge and requires skill in balancing maternal management, fetal care, and oncological treatment. Cisplatin has been routinely used as a first line agent for neoadjuvant chemotherapy in this situation, even though it has also recently been associated with fetal hearing loss. We report a case of stage IB3 cervical cancer diagnosed at 17 gestational weeks successfully treated with neoadjuvant chemotherapy using carboplatin and paclitaxel during pregnancy. Carboplatin is a valid alternative to cisplatin for advanced stage cervical carcinoma in a pregnant patient, in particular in view of the neonatal complications (primarily ototoxicity) that are associated with in utero cisplatin exposure.
- Published
- 2020
38. Electrochemically Reduced Graphene Oxide as Screen‐printed Electrode Modifier for Fenamiphos Determination
- Author
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K. Fernandes, Márcio F. Bergamini, E. Y. Watanabe, Maurício A.P. Papi, Luiz H. Marcolino-Junior, Craig E. Banks, and Ava Gevaerd
- Subjects
chemistry.chemical_compound ,Materials science ,chemistry ,Chemical engineering ,Graphene ,law ,Screen printed electrode ,Electrochemistry ,Oxide ,Fenamiphos ,Analytical Chemistry ,law.invention - Published
- 2020
39. Segregation of caffeine reward and aversion in the rat nucleus accumbens shell versus core
- Author
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Michael Bergamini, Michal Chwalek, Mandy Yee, Isabel Mackay-Clackett, Derek van der Kooy, Ryan Ting-A-Kee, Geith Maal-Bared, and Taryn E. Grieder
- Subjects
Male ,Nucleus accumbens ,Pharmacology ,Nucleus Accumbens ,Rats, Sprague-Dawley ,03 medical and health sciences ,chemistry.chemical_compound ,0302 clinical medicine ,Eticlopride ,Reward ,Dopamine ,Caffeine ,medicine ,Animals ,Rats, Wistar ,Receptor ,030304 developmental biology ,0303 health sciences ,Chemistry ,General Neuroscience ,Psychoactive drug ,Rats ,Ventral tegmental area ,medicine.anatomical_structure ,Dopamine receptor ,030217 neurology & neurosurgery ,medicine.drug - Abstract
Caffeine, the most commonly consumed psychoactive drug in the world, is readily available in dietary sources, including soft drinks, chocolate, tea and coffee. However, little is known about the neural substrates that underlie caffeine's rewarding and aversive properties and what ultimately leads us to seek or avoid caffeine consumption. Using male Wistar rats in a place conditioning procedure, we show that systemic caffeine at a low intraperitoneal dose of 2 mg/kg (or 100 µM injected directly into the rostral, but not caudal, portion of the ventral tegmental area) produced conditioned place preferences. By contrast, high doses of systemic caffeine at 10 and 30 mg/kg produced conditioned place aversions. These aversions were not recapitulated by a caffeine analog restricted to the periphery. Both caffeine reward and aversion were blocked by systemic D1-like receptor antagonism using SCH23390, while systemic D2-like receptor antagonism with eticlopride had smaller effects on caffeine motivation. Most important, we demonstrated that pharmacological blockade of dopamine receptors using α-flupenthixol injected into the nucleus accumbens shell, but not core, blocked caffeine-conditioned place preferences. Conversely, α-flupenthixol injected into the nucleus accumbens core, but not shell, blocked caffeine-conditioned place aversions. Thus, our findings reveal two dopamine-dependent and functionally dissociable mechanisms for processing caffeine motivation, which are segregated between nucleus accumbens subregions. These data provide novel evidence for the roles of the nucleus accumbens subregions in mediating approach and avoidance behaviours for caffeine.
- Published
- 2020
40. Heparin‐induced lipoprotein precipitation apheresis in dyslipidemic patients: A multiparametric assessment
- Author
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Erminia Di Bartolomeo, Tommaso Fasano, Chiara Marraccini, Roberto Baricchi, Thelma A. Pertinhez, Daniela Farioli, Alessia Latorrata, Emanuela Monari, Aldo Tomasi, Lucia Merolle, Elisa Bellei, Stefania Bergamini, Luca Scarano, and Eleonora Quartieri
- Subjects
Male ,Apolipoprotein B ,Lipoproteins ,030204 cardiovascular system & hematology ,Pharmacology ,proton nuclear magnetic resonance ,Mass Spectrometry ,03 medical and health sciences ,chemistry.chemical_compound ,proteomics ,0302 clinical medicine ,cytokines, metabolomics, PCSK-9, proteomics, proton nuclear magnetic resonance ,medicine ,Humans ,Metabolomics ,Electrophoresis, Gel, Two-Dimensional ,Aged ,Dyslipidemias ,Inflammation ,Proteomic Profile ,biology ,Heparin ,Viscosity ,Cholesterol ,business.industry ,PCSK9 ,Hematology ,General Medicine ,Middle Aged ,medicine.disease ,metabolomics ,cytokines ,Biomechanical Phenomena ,PCSK-9 ,Apheresis ,chemistry ,Blood Component Removal ,Quality of Life ,biology.protein ,Female ,lipids (amino acids, peptides, and proteins) ,Proprotein Convertase 9 ,business ,Dyslipidemia ,Lipoprotein(a) ,030215 immunology ,Lipoprotein ,medicine.drug - Abstract
Low-density lipoprotein (LDL) apheresis (LA) selectively eliminates lipoproteins containing apolipoprotein B 100 (ApoB100) on patients affected by severe dyslipidemia. In addition to lowering lipids, LA is thought to exert pleiotropic effects altering a number of other compounds associated with atherosclerosis, such as pro- and anti-inflammatory cytokines or pro-thrombotic factors. More knowledge needs to be gathered on the effects of LA, and particularly on its ability to modify blood components other than lipids. We performed a multiparametric assessment of the inflammatory, metabolic and proteomic profile changes after Heparin-induced lipoprotein precipitation (H.E.L.P.) apheresis on serum samples from nine dyslipidemic patients evaluating cholesterol and lipoproteins, plasma viscosity and density, metabolites, cytokines, PCSK9 levels and other proteins selectively removed after the treatment. Our results show that H.E.L.P. apheresis is effective in lowering lipoprotein and PCSK9 levels. Although not significantly, complement and inflammation-related proteins are also affected, indicating a possible transient epiphenomenon induced by the extracorporeal procedure.
- Published
- 2020
41. Patients with adenoid cystic carcinomas of the salivary glands treated with lenvatinib: Activity and quality of life
- Author
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Roberta Granata, Salvatore Lo Vullo, Carlo Resteghini, Donata Galbiati, Pasquale Quattrone, Salvatore Alfieri, Luigi Mariani, Cristiana Bergamini, Francesca Platini, Susanne Singer, Lisa Licitra, Ester Orlandi, Laura D. Locati, Giuseppina Calareso, Moela Mancinelli, Stefano Cavalieri, and Paolo Bossi
- Subjects
Adult ,Male ,adenoid cystic carcinoma ,lenvatinib ,quality of life ,toxicity ,Cancer Research ,medicine.medical_specialty ,Adenoid cystic carcinoma ,Antineoplastic Agents ,03 medical and health sciences ,chemistry.chemical_compound ,0302 clinical medicine ,Swallowing ,Quality of life ,Internal medicine ,medicine ,Clinical endpoint ,Humans ,Prospective Studies ,030212 general & internal medicine ,Neoplasm Metastasis ,Protein Kinase Inhibitors ,Dose-Response Relationship, Drug ,business.industry ,Phenylurea Compounds ,Cancer ,Common Terminology Criteria for Adverse Events ,Middle Aged ,Salivary Gland Neoplasms ,medicine.disease ,Carcinoma, Adenoid Cystic ,Survival Analysis ,Salivary Gland Adenoid Cystic Carcinoma ,Oncology ,chemistry ,030220 oncology & carcinogenesis ,Quinolines ,Female ,Neoplasm Recurrence, Local ,business ,Lenvatinib - Abstract
The treatment of patients with recurrent and/or metastatic (R/M) salivary gland adenoid cystic carcinoma (ACC) remains an unmet need.Patients with R/M disease with a history of clinical or symptomatic disease progression within 6 months and a maximum of 1 previous line of chemotherapy or a multiple kinase inhibitor received oral lenvatinib at a dose of 24 mg/day. The primary endpoint was the objective response rate; secondary endpoints included quality of life (QOL) (according to the European Organization for Research and Treatment of Cancer Quality of Life Questionnaire-Core 30 Items [EORTC QLQ-C30] and the European Organization for Research and Treatment of Cancer Quality of Life Questionnaire-Core Module Head and Neck Module [EORTC QLQ-HN35]), progression-free survival and overall survival, duration of response, and toxicities.Twenty-eight patients with R/M ACC were enrolled. Among 26 evaluable patients, 3 partial responses (11.5%) were reported. Target lesion reductions between 23% to 28% were observed in 4 of 20 patients with stable disease. Treatment-related adverse events were frequent (all grades, 96%; grade≥3 in 50% of cases according to version 4.03 of the National Cancer Institute Common Terminology Criteria for Adverse Events). The dose of lenvatinib was reduced in 24 patients, whereas in 21 patients the dose was reduced within the first 12 weeks and 4 patients maintained the full dose throughout treatment. The QOL deteriorated between baseline and 6 months with regard to Fatigue and Dry Mouth. There was no evidence of changes in Swallowing and Physical Functioning. At a median follow-up of 29 months, 2 patients remained on treatment, 10 patients were off protocol for disease progression and were alive with disease, and 14 patients had died of disease progression. The median overall survival, progression-free survival, and duration of response were 27 months, 9.1 months, and 3.1 months, respectively.Lenvatinib appears to have modest activity in ACC. Toxicities are common but manageable and QOL was found to deteriorate in some domains.
- Published
- 2020
42. Identifying drug targets in tissues and whole blood with thermal-shift profiling
- Author
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Nils Kurzawa, Marcus Bantscheff, Mikhail M. Savitski, Eugenia Stonehouse, Maria Faelth-Savitski, Holger Franken, Bianca Heller, Christina Rau, Jessica Perrin, Anna Rutkowska, H. Christian Eberl, Johanna Vappiani, Daniel C. Sévin, Isabelle Becher, Katrin Strohmer, Jana Krause, Thilo Werner, Douglas W. Thomson, Wolfgang Huber, Daniel Poeckel, Giovanna Bergamini, Mathias Kalxdorf, and Dorothee Childs
- Subjects
Male ,Proteome ,medicine.drug_class ,Biomedical Engineering ,Bioengineering ,Drug action ,Kidney ,Applied Microbiology and Biotechnology ,Mass Spectrometry ,Small Molecule Libraries ,Mice ,03 medical and health sciences ,chemistry.chemical_compound ,0302 clinical medicine ,In vivo ,Panobinostat ,Testis ,medicine ,Animals ,Humans ,Vemurafenib ,Lung ,Transcription factor ,030304 developmental biology ,Whole blood ,0303 health sciences ,Protein Stability ,Chemistry ,Histone deacetylase inhibitor ,Kidney metabolism ,Azepines ,Hep G2 Cells ,Triazoles ,Rats ,Cell biology ,Blood ,Liver ,Organ Specificity ,Thermodynamics ,Molecular Medicine ,Spleen ,030217 neurology & neurosurgery ,Biotechnology ,medicine.drug - Abstract
Monitoring drug-target interactions with methods such as the cellular thermal-shift assay (CETSA) is well established for simple cell systems but remains challenging in vivo. Here we introduce tissue thermal proteome profiling (tissue-TPP), which measures binding of small-molecule drugs to proteins in tissue samples from drug-treated animals by detecting changes in protein thermal stability using quantitative mass spectrometry. We report organ-specific, proteome-wide thermal stability maps and derive target profiles of the non-covalent histone deacetylase inhibitor panobinostat in rat liver, lung, kidney and spleen and of the B-Raf inhibitor vemurafenib in mouse testis. In addition, we devised blood-CETSA and blood-TPP and applied it to measure target and off-target engagement of panobinostat and the BET family inhibitor JQ1 directly in whole blood. Blood-TPP analysis of panobinostat confirmed its binding to known targets and also revealed thermal stabilization of the zinc-finger transcription factor ZNF512. These methods will help to elucidate the mechanisms of drug action in vivo.
- Published
- 2020
43. A carbon fiber ultramicroelectrode as a simple tool to direct antioxidant estimation based on caffeic acid oxidation
- Author
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Ava Gevaerd, Paulo Roberto de Oliveira, Márcio F. Bergamini, Bruna Medeiros da Silva, and Luiz Humberto Marcolino Junior
- Subjects
Detection limit ,Auxiliary electrode ,Working electrode ,Chemistry ,Supporting electrolyte ,General Chemical Engineering ,010401 analytical chemistry ,General Engineering ,Analytical chemistry ,Ultramicroelectrode ,02 engineering and technology ,Standard solution ,021001 nanoscience & nanotechnology ,01 natural sciences ,0104 chemical sciences ,Analytical Chemistry ,Diffusion layer ,Electrode ,0210 nano-technology - Abstract
This work describes the construction and evaluation of carbon fiber ultramicroelectrodes (CF-UMEs) in the voltammetric estimation of the antioxidant capacity of wine and grape samples based on caffeic acid (HCAF) oxidation. For this, lab-made CF-UMEs were constructed using an arrangement of six carbon fibers (7 μm diameters individual) assembled in a glass capillary, and caffeic acid (HCAF) was used as a standard solution. By using the most straightforward 2-electrode cell arrangement (the CF-UME as a working electrode and Ag/AgCl as a reference/auxiliary electrode), voltammetric measurements of a 1.0 mmol L-1 HCAF solution were done in the absence of a supporting electrolyte. A sigmoidal voltammetric profile was observed in CF-UMEs caused by a more effective mass transport by radial diffusion, which leads to a rapid formation of the diffusion layer. Reproducibility studies for different 6-fiber electrodes manually constructed in different batches showed an RSD of less than 5%. For the same electrode surface, a variation of 2.7% was observed. Under optimized conditions, a linear relationship between anodic peak current and HCAF concentration from 3.0 to 500 μmol L-1 with a sensitivity of 12 μA L mol-1 was reached. The limits of detection (LOD) and quantification (LOQ) were calculated to be 0.41 and 1.26 μmol L-1, respectively. The proposed electrochemical method was applied in the estimation of the antioxidant capacity in three different wine samples as well as in green and red grapes. Concordant and satisfactory results by comparison with a proper method were obtained, which suggests that the proposed sensor can be successfully applied for direct analysis of wine and grape samples by estimation of HCAF content.
- Published
- 2020
44. Chemical Wet Oxidation of Carbon Nanotubes for Electrochemical Determination of Methyl Parathion
- Author
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Maurício A.P. Papi, Emily Y. Watanabe, Michelle C. Oliveira, Fabio R. Caetano, Márcio F. Bergamini, and Luiz H. Marcolino-Júnior
- Subjects
Chemical substance ,Chemistry ,010401 analytical chemistry ,Inorganic chemistry ,Sulfuric acid ,Carbon nanotube ,010402 general chemistry ,Electrochemistry ,01 natural sciences ,0104 chemical sciences ,Analytical Chemistry ,law.invention ,symbols.namesake ,chemistry.chemical_compound ,law ,Electrode ,symbols ,Wet oxidation ,Fourier transform infrared spectroscopy ,Raman spectroscopy - Abstract
Surface properties of carbon nanotubes in electrochemical sensors can be dramatically altered depending on chemical groups and structural defects present on the surface. The effect of oxidation on structural integrity of multiwalled carbon nanotubes through acidic (nitric/sulfuric acids) and basic (sodium hydroxide) agents has been studied. Chemical modifications of carbon nanotubes structures were evaluated by Raman, Fourier transform infrared spectroscopy and transmission electronic microscopy. Furthermore, these materials were used to construct sensors for electrochemical determination of pesticide methyl parathion (MP). Under optimized conditions, a calibration curve was obtained for MP determination employing a glassy carbon electrode modified with acidic treated and basic treated carbon nanotubes which showed a linear response ranging from 1.0 × 10–7 to 3.4 × 10–5 M with limits of detection of 3.3 × 10–8 and 3.5 ×10–8 M, respectively. Analytical performance of the electrodes showed similar voltammetric behavior providing good sensitivity for the determination of methyl parathion.
- Published
- 2020
45. Optimization of Orally Bioavailable PI3Kδ Inhibitors and Identification of Vps34 as a Key Selectivity Target
- Author
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Giovanna Bergamini, Stefano Livia, Kenneth David Down, Marcus Bantscheff, Nick Barton, Friedrich B M Reinhard, Paola Grandi, Daniel Thomas, David N. Mallett, Paul Martin Gore, Sophie M. Bertrand, Z.A. Henley, Edith M. Hessel, James E. Rowedder, Mark Price, J. Nicole Hamblin, Birgit Dümpelfeld, Augustin Amour, Steve Keeling, Christina Rau, Máire A. Convery, Chris D. Edwards, Jonathan A. Taylor, Paul Rowland, and Aoife C. Maxwell
- Subjects
Models, Molecular ,Cell Membrane Permeability ,Cell membrane permeability ,Class I Phosphatidylinositol 3-Kinases ,Biological Availability ,Class iii ,Crystallography, X-Ray ,Binding, Competitive ,Structure-Activity Relationship ,Drug Discovery ,Animals ,Humans ,Structure–activity relationship ,Potency ,Phosphoinositide-3 Kinase Inhibitors ,Drug discovery ,Chemistry ,Class III Phosphatidylinositol 3-Kinases ,Combinatorial chemistry ,Rats ,Bioavailability ,Isoenzymes ,Molecular Docking Simulation ,Toxicity ,Molecular Medicine ,Selectivity - Abstract
Optimization of a lead series of PI3Kδ inhibitors based on a dihydroisobenzofuran core led to the identification of potent, orally bioavailable compound 19. Selectivity profiling of compound 19 showed similar potency for class III PI3K, Vps34, and PI3Kδ, and compound 19 was not well-tolerated in a 7-day rat toxicity study. Structure-based design led to an improvement in selectivity for PI3Kδ over Vps34 and, a focus on oral phramacokinetics properties resulted in the discovery of compound 41, which showed improved toxicological outcomes at similar exposure levels to compound 19.
- Published
- 2019
46. Quick electrochemical immunoassay for hantavirus detection based on biochar platform
- Author
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Luiz H. Marcolino-Junior, Claudia Nunes Duarte dos Santos, Márcio F. Bergamini, Fabio R. Caetano, Jeferson L. Gogola, Taissa Ricciardi Jorge, Cristiane Kalinke, and Gustavo Martins
- Subjects
Orthohantavirus ,Quick Test ,Covalent binding ,02 engineering and technology ,Antibodies, Viral ,01 natural sciences ,Analytical Chemistry ,Viral Proteins ,Limit of Detection ,Biochar ,medicine ,Humans ,Electrodes ,Hantavirus ,Immunoassay ,biology ,medicine.diagnostic_test ,Chemistry ,010401 analytical chemistry ,Reproducibility of Results ,Electrochemical Techniques ,021001 nanoscience & nanotechnology ,Serum samples ,Virology ,0104 chemical sciences ,Blood ,Charcoal ,Immunoglobulin G ,Electrochemical immunoassay ,biology.protein ,Antibody ,0210 nano-technology ,Antibodies, Immobilized - Abstract
This work describes the first method using biochar (BC) as carbonaceous platform for immunoassay application. BC is a highly functionalized material obtained through biomass pyrolysis under controlled conditions. Due to the highly functionalized surface, covalent binding between BC and biomolecules can be performed by EDC/NHS conjugation. The application of the modified electrode was done with Hantavirus, that are etiologic agents mainly transmitted by wild rodents. Among its pathologies Hantavirus Cardiopulmonary Syndrome (HCPS) arises at Americas, caused by Hantavirus Araucaria and reaches 40% lethality. The diagnostic is based on the presence of specific hantavirus nucleoprotein (Np), under viremic condition or IgG2b antibodies (Ab), during first symptoms. The results presented a device sensitivity of 5.28 μA dec-1 and a LOD of 0.14 ng mL-1 to the Np detection, ranging from 5.0 ng mL-1 to 1.0 μg mL-1, the Ab detection works as qualitative type sensor above 200 ng mL-1. Both sensors were evaluated its selectivity and serum samples; selectivity against Gumboro disease, VP2 protein, and antibody IgG2a against Yellow fever disease (YF), respectively. So, the devices here proposed are promising tool suitable for both rodent and human hantavirus clinical surveys.
- Published
- 2019
47. Photoelectrochemistry at semiconductor/liquid interfaces triggered by electrochemiluminescence
- Author
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Jean-François Bergamini, Neso Sojic, Yoan Léger, Gabriel Loget, Jing Yu, Yiran Zhao, Centre National de la Recherche Scientifique (CNRS), Institut des Sciences Chimiques de Rennes (ISCR), Centre National de la Recherche Scientifique (CNRS)-Institut de Chimie du CNRS (INC)-Université de Rennes 1 (UR1), Université de Rennes (UNIV-RENNES)-Université de Rennes (UNIV-RENNES)-Ecole Nationale Supérieure de Chimie de Rennes (ENSCR)-Institut National des Sciences Appliquées - Rennes (INSA Rennes), Institut National des Sciences Appliquées (INSA)-Université de Rennes (UNIV-RENNES)-Institut National des Sciences Appliquées (INSA), Université de Bordeaux (UB), Institut des Fonctions Optiques pour les Technologies de l'informatiON (Institut FOTON), Université de Rennes 1 (UR1), Université de Rennes (UNIV-RENNES)-Université de Rennes (UNIV-RENNES)-Institut National des Sciences Appliquées - Rennes (INSA Rennes), Institut National des Sciences Appliquées (INSA)-Université de Rennes (UNIV-RENNES)-Institut National des Sciences Appliquées (INSA)-École Nationale Supérieure des Sciences Appliquées et de Technologie (ENSSAT)-Centre National de la Recherche Scientifique (CNRS)-IMT Atlantique Bretagne-Pays de la Loire (IMT Atlantique), Institut Mines-Télécom [Paris] (IMT)-Institut Mines-Télécom [Paris] (IMT), Université de Rennes (UR)-Institut National des Sciences Appliquées - Rennes (INSA Rennes), Institut National des Sciences Appliquées (INSA)-Institut National des Sciences Appliquées (INSA)-Ecole Nationale Supérieure de Chimie de Rennes (ENSCR)-Institut de Chimie du CNRS (INC)-Centre National de la Recherche Scientifique (CNRS), Institut National des Sciences Appliquées (INSA)-Institut National des Sciences Appliquées (INSA)-École Nationale Supérieure des Sciences Appliquées et de Technologie (ENSSAT)-Centre National de la Recherche Scientifique (CNRS), ANR-20-CE29-0006, ANR, China Scholarship Council, and ANR-20-CE29-0006,LiCORN,Conversion lumineuse par électrochimiluminescence photoinduite(2020)
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Silicon ,Materials science ,QC1-999 ,Photoelectrochemistry ,General Physics and Astronomy ,chemistry.chemical_element ,02 engineering and technology ,010402 general chemistry ,01 natural sciences ,7. Clean energy ,law.invention ,law ,Photoelectrodes ,[CHIM]Chemical Sciences ,Electrochemiluminescence ,General Materials Science ,Diode ,business.industry ,Physics ,General Engineering ,General Chemistry ,021001 nanoscience & nanotechnology ,Laser ,0104 chemical sciences ,General Energy ,Semiconductor ,chemistry ,Semiconductors ,Optoelectronics ,Light emission ,Solar simulator ,0210 nano-technology ,business - Abstract
International audience; The photoelectrochemical charge-transfer process occurring at semiconductor surfaces has important implications in the fields of solar fuels and biodetection. Usually, physical light sources located outside the liquid phase, such as a solar simulator, a light-emitting diode (LED), or a laser, are used for photoelectrochemical studies. Here, we report inducing photoelectrochemistry using an electrochemical source of light, that is, the electrochemiluminescence (ECL) emitted by a model co-reactant system. Results reveal that the ECL illumination can activate several semiconductor (SC) photoelectrodes based on n-type Si, n-type GaAs, and p-type Si. We demonstrate that this emitter-receiver concept, based on dual-light conversion, enables photoelectrochemical charge transfer at the solid/liquid interface, which correlates with the ECL intensity. The singularities of this concept lie in the fact that light emission and collection both occur in the liquid phase, that ECL is an easily miniaturizable photon source, and that the SC/liquid junction can be easily implemented. This approach may open perspectives for remote ECL detection strategies and original photoelectrochemical analytical systems.
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- 2021
48. Somatic Embryogenesis in Vitis for Genome Editing: Optimization of Protocols for Recalcitrant Genotypes
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Teodora Basile, Maria Francesca Cardone, Flavia Angela Maria Maggiolini, Carlo Bergamini, Riccardo Velasco, Antonio Domenico Marsico, Lucia R Forleo, and M. D'Amico
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new breeding techniques ,genetic improvement ,Somatic embryogenesis ,fungi ,Plant culture ,food and beverages ,table grapes ,Plant Science ,Vitis vinifera L ,Horticulture ,Sterilization (microbiology) ,Biology ,SB1-1110 ,chemistry.chemical_compound ,Transformation (genetics) ,chemistry ,Callus ,Genotype ,Plant breeding ,Citric acid ,embryogenic callus - Abstract
New Plant Breeding Techniques (NPBTs) protocols have been developed to produce new grape varieties with improved quantitative and qualitative characteristics. Reliable transformation protocols for grapes are based on the generation/induction of embryogenic callus cells that are then transformed. Varieties such as Italia have proven to be very recalcitrant to regeneration via somatic embryogenesis. In this work, the development of a protocol for improved production of embryogenic calluses is described. Two sterilization protocols were tested: (a) a lower active chlorine concentration for a longer time (LS), and (b) a higher chlorine concentration for a shorter time (HS), in combination with the absence or presence of citric acid in the growing substrate in the first growth media. The embryogenic calluses formation in Chardonnay, a cv. with a high embryogenic response, was significantly higher in presence of citric acid in the initial growing substrate regardless of the sterilization protocol. In Aglianico, a cv. with a lower embryogenic response, no significant differences were observed. Instead, in a recalcitrant cv. as Italia, we obtained a 13-fold increase in embryogenic calluses formation performing sterilization of flowers with the HS protocol compared to LS.
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- 2021
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49. The Motility and Mesenchymal Features of Breast Cancer Cells Correlate with the Levels and Intracellular Localization of Transglutaminase Type 2
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Jeffrey W. Keillor, Gianluca Aguiari, Carlo M. Bergamini, Karine Bourgeois, Silvia Grassilli, Stefano Volinia, Federica Brugnoli, Nicoletta Bianchi, and Valeria Bertagnolo
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Epithelial-Mesenchymal Transition ,Transcription, Genetic ,QH301-705.5 ,Tissue transglutaminase ,Intracellular Space ,Motility ,Socio-culturale ,Vimentin ,Breast Neoplasms ,Article ,Fluorescence ,Mesoderm ,breast cancer ,Cell Movement ,Cell Line, Tumor ,Gene expression ,medicine ,Humans ,Doxorubicin ,Neoplasm Invasiveness ,Protein Glutamine gamma Glutamyltransferase 2 ,Biology (General) ,Cytoskeleton ,Cytotoxicity ,skin and connective tissue diseases ,Cell Shape ,NC9 ,Cell Nucleus ,biology ,Chemistry ,Mesenchymal stem cell ,EMT ,General Medicine ,Cadherins ,motility ,transglutaminase type 2 ,Cancer research ,biology.protein ,Female ,medicine.drug - Abstract
We have investigated motility in breast cancer cell lines in association with the expression of Transglutaminase type 2 (TG2) as well as upon the administration of Doxorubicin (Dox), an active cytotoxic agent that is employed in chemotherapy. The exposure of MCF-7 cells to the drug increased TG2 levels, triggering epithelial–mesenchymal transition (EMT), thereby supporting cell motility. The effects of Dox on the movement of MCF-7 cells were counteracted by treatment with NC9, a TG2 inhibitor, which induced morphological changes and also reduced the migration of MDA-MB-231 cells exhibiting high levels of TG2. The physical association of TG2 with the cytoskeletal component vimentin appeared pivotal both in drug-treated MCF-7 and in MDA-MB-231 cells and seemed to be independent of the catalytic activity of TG2. NC9 altered the subcellular distribution of TG2 and, consequently, the co-localization of TG2 with vimentin. Furthermore, NC9 induced a nuclear accumulation of TG2 as a prelude to TG2-dependent gene expression modifications. Since enzyme activity can affect both motility and nuclear functions, targeting of this protein could represent a method to improve therapeutic interventions in breast tumors, particularly those to control progression and to limit drug resistance.
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- 2021
50. Disposable and low-cost lab-made screen-printed electrodes for voltammetric determination of L-dopa
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Luiz H. Marcolino-Junior, Jeferson L. Gogola, Jefferson H.S. Carvalho, Márcio F. Bergamini, and Bruno C. Janegitz
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Detection limit ,Materials science ,QA71-90 ,Cellulose acetate ,Square wave ,Instruments and machines ,Atomic and Molecular Physics, and Optics ,Electronic, Optical and Magnetic Materials ,Levodopa ,Polyvinyl chloride ,chemistry.chemical_compound ,Conductive ink ,chemistry ,Electrode ,Disposable electrochemical sensors ,Computer Science (miscellaneous) ,Electrical and Electronic Engineering ,Fourier transform infrared spectroscopy ,Cyclic voltammetry ,Instrumentation ,Nuclear chemistry - Abstract
This work presents a feasible process for preparation screen-printed electrode (SPE) using a lab-made conductive ink based on cellulose acetate (CAc) and graphite powder (GP) printed on polyvinyl chloride (PVC) sheet. The CAc present in the composition of this SPE is a binding agent for conductive inks with GP, which are relatively low-cost and easily obtainable materials, compared to other similar commercial sensors. The SPE was evaluated through cyclic voltammetry (CV) using an equimolar [Fe(CN)6]3-/4− solution as a redox probe to find an adequate ink composition. Electrode surface was characterized by scanning electron microscopy (SEM) and Fourier transform infrared spectroscopy (FTIR). To show the analytical potential of SPE proposed it was used for Levodopa (L-dopa) determination. L-dopa is a drug frequently used to reduce the symptoms of Parkinson's disease. The GP-CAc/PVC electrode was evaluated for the determination of L-dopa under square wave voltammetric (SWV) conditions. The electrode presented a linear dynamic range (LDR) from 8.00 to 100 µmol L−1 and a limit of detection (LOD) of 0.06 µmol L−1. As a proof-of-concept, the proposed electrode was used to quantify L-dopa in drug samples as an alternative tool for quality control tests, being applied to two commercial drugs. The electrode construction strategy proved to be reproducible with an analytical performance comparable to other similar electrodes reported in the literature.
- Published
- 2021
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