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Introduction: Benign sebaceous salivary gland (SG) neoplasms represent approximately 0.2% of all salivary gland neoplasms. Not only are fine needle aspiration (FNA) biopsy findings of sebaceous adenoma (SA) and sebaceous lymphadenoma (SLA) limited, but their findings are also rarely compared with one another. Materials and Methods: Our cytopathology files were searched for examples of benign sebaceous SG neoplasms with concomitant histopathological verification. FNA biopsy and cell collection were performed using standard technique. Results: One case each of parotid SA and parotid SLA showed markedly dissimilar cytomorphology. The SA case was composed of a repetitive population of profusely multivacuolated polygonal cells with single and multiple nuclei, and was specifically recognised cytologically as a sebaceous neoplasm due to its characteristic cytoplasmic vacuolisation. The SLA case, however, was characterised by smears filled primarily with lymphocytes and only scant widely scattered basaloid cell clusters. A non‐specific diagnosis of basaloid neoplasm was rendered. In retrospect, recognition of sebaceous differentiation was limited to rare cell groups. Conclusion: Though nominally, epidemiologically, and to a degree histopathologically analogous, the cytopathology of SA and SLA are markedly dissimilar, reflecting the dominant cell component in each. With FNA biopsy, a specific interpretation is more likely for SA than SLA due to the overwhelming obscuring lymphoid population in the latter. [ABSTRACT FROM AUTHOR]

Introduction: Pleomorphic dermal sarcoma (PDS) is an uncommon cutaneous mesenchymal neoplasm. It is cytomorphologically identical to atypical fibroxanthoma (AFX), but differs due to its invasion beyond the dermis. We undertook an examination of our experience with fine needle aspiration (FNA) biopsy cytology of PDS. Materials and Methods: Our cytopathology files were searched for examples of PDS with concomitant histopathological verification. FNA biopsy smears and cell collection were performed using standard techniques. Results: Seven cases of PDS were retrieved from four different patients (M:F, 1:1; age range: 63–88 years; mean age = 78 years). All patients (57%) presented with a primary tumour with one having an FNA biopsy of two local recurrences and a single distant metastasis. Five aspirates were from the extremities and two from the head/neck. Tumours ranged from 1.0 to 3.5 cm (mean, 2.2 cm). Specific cytological diagnoses were pleomorphic spindle/epithelioid sarcoma (3 cases), PDS (2), AFX (1), and atypical myofibroblastic lesion, query nodular fasciitis (1). Immunohistochemical (IHC) staining from FNA‐generated cell blocks in two cases showed non‐specific staining with vimentin in both cases; positive CD10, CD68, and INI‐1 staining in one case; and smooth muscle actin expression in the other. Multiple negative stains were performed in both of these cases to exclude malignant melanoma, carcinoma, and specific forms of sarcoma. Cytopathology consisted of a mixture of spindle, epithelioid, and bizarre pleomorphic cells. Conclusion: Coupled with ancillary IHC stains, FNA biopsy can help recognise PDS as a sarcomatous cutaneous neoplasm, but is unable to distinguish PDS from AFX. [ABSTRACT FROM AUTHOR]

Introduction: Atypical spindle cell/pleomorphic lipomatous tumour (ASPLT) is an infrequently appreciated benign lipomatous neoplasm newly accepted into the most recent WHO classification of soft tissue tumours. Materials and Methods: Our cytopathology files were searched for examples of ASPLT and spindle cell/pleomorphic lipoma (SCPL) having histopathological verification. Conventional fine needle aspiration (FNA) biopsy smears were performed using standard techniques. Results: Eleven patients including three cases of ASPLT and eight of SCPL (M:F = 4.5:1; age range: 39‐97 years, mean age = 60 years) met the inclusion criteria. FNA biopsy sites included extremity (5, 45%), trunk (3, 27%), and head/neck (3, 27%). All aspirates were from primary neoplasms. FNA diagnoses of ASPLT cases were spindle cell lipomatous neoplasm, fibrotic low‐grade SC neoplasm, and myxoid lipomatous neoplasm. Eight SCPL cases were diagnosed as spindle cell neoplasm (3), spindle cell lipoma (SCL) (1), pleomorphic lipoma (1), suspicious for SCL (1), benign adipose tissue (1), and benign spindle cells and connective tissue (1). Ancillary testing in two ASPLT cases showed positive CD34 and negative MDM2 immunostain in one, and negative FISH results for MDM2 and DDIT3 in another. Conclusion: ASPLT is a novel lipomatous neoplasm simulating primarily SCPL and atypical lipoma/well‐differentiated liposarcoma. Diligent cytomorphological observation, clinical information, and ancillary testing may allow for its specific recognition using FNA biopsy. [ABSTRACT FROM AUTHOR]

Introduction: The cytopathology and diagnostic accuracy of salivary gland (SG) polymorphous adenocarcinoma (PAC) is the subject of a limited number of reports. We undertook a review of our experience with fine needle aspiration (FNA) biopsy and PAC. Materials and methods: A search was made of our cytopathology files for PAC cases that also had histopathological confirmation. FNA biopsy smears and cell‐blocks were performed and examined using standard techniques. Results: Eight FNA biopsy cases of histologically proven PAC from 7 patients [F:M = 1.3:1, age 39‐75 years, mean = 58] met study inclusion. Metastatic aspirates were most common (4), followed by 3 primary cases and 1 locally recurrent neoplasm. Primary FNA sites included hard palate (1 case), lip (1), and lateral tongue (1); all metastatic sites were in the neck. A precise cytologic diagnosis was made in 38% of cases; however, when applying the Milan classification system, 100% could be categorised as either malignant or of uncertain malignant potential. Ancillary immunohistochemical testing performed in 44% of the cases was non‐specific. Cytologic smears showed cellular uniformity and structural variety of cell groups with tubular, branching, cribriform, and convex patterns as well as variable, but occasionally abundant globular myxoid stroma leading to confusion with adenoid cystic carcinoma. Conclusion: The imitative cytopathology of PAC with other SG neoplasms as well as its infrequency in routine FNA biopsy practice makes specific interpretation difficult, but using a classification system allows for appropriate patient management. Molecular testing in future specimens holds promise for enhancing diagnostic accuracy. [ABSTRACT FROM AUTHOR]

Abstract: We report an example of an esophageal giant fibrovascular polyp harboring a well-differentiated liposarcoma with rhabdomyomatous differentiation in a 68 year-old man. Initial imaging studies including fiberoptic endoscopy and 2 computed tomographic examinations, and laparoscopic myotomy failed to demonstrate the polypoid intraluminal nature of the lesion. Thus, alternative diagnoses including a malignant neoplasm and achalasia were entertained. A third computed tomography with contrast showed a pedunculated mass. Surgical resection revealed a 15-cm smooth-surfaced club-shaped mass composed of a well-differentiated liposarcoma demonstrating myoglobin, muscle-specific actin, and myogenin-positive rhabdomyomatous differentiation. To date, no previously reported giant fibrovascular polyp case has described a liposarcoma with rhabdomyomatous change. [Copyright &y& Elsevier]

The article discusses a study on the reliability of fine-needle aspiration (FNA) for high-grade sarcoma (HGS). The researchers examined their cytopathology database from March 2001 to January 2007, and determined all FNA cases diagnosed as HGS. It was found out that a cytopathologic diagnose of HGS was accurate in 88 of 97 cases with follow-up. Moreover, a FNA biopsy diagnose of HGS seems to be clinically reliable in a number of cases when used in collaboration with the orthopedic team.

BACKGROUND. Extraskeletal myxoid chondrosarcoma (EMC) is a rare soft-tissuesarcoma rarely subjected to cytologic analysis. With the exception of a few small series, the cytology literature of EMC is largely limited to single-case reports. The purpose was to evaluate the cytomorphology of 8 EMC cases as obtained by imprint/scrape cytology and fine-needle aspiration (FNA) biopsy, to review the literature,and to demonstrate the utility of cytogenetic analysis in the diagnosis of EMC.METHODS. The cytology files were reviewed for all soft-tissue lesions signed out aschondrosarcoma, myxoid sarcoma, and EMC, and the tissue files for any cases ofEMC that had corresponding cytopathology. FNA was performed using a standardtechnique. Scrape preparations were performed from tissue sent fresh to the laboratory for either frozen section or for special studies such as electron microscopy ortissue banking.RESULTS. Eight cases of EMC were retrieved from 4 men and 3 women (medianage 5 62 years). One patient had 2 separate cytologic specimens 4.5 years apart. Allpatients had subsequent tissue confirmation of the diagnosis of EMC. Five individuals presented as new patients, and 2 had a prior diagnosis of EMC. Sites included4masses from the foot/ankle, 2 from the calf, 1 wrist mass, and 1 buttock mass. Fivepatients were diagnosed from FNA biopsy, whereas 3 were diagnosed using scrapeslides. Five cases were correctly and categorically diagnosed by the cytologicmethod as EMC, 1 as chondrosarcoma favor EMC, 1 as sarcoma favor EMC, and 1as myxoid spindle/epithelial neoplasm. Cytologic features ranged from hypocellularto highly cellular smears composed primarily of rounded cells set in an abundantmyxoid stroma that varied from opaque to semitransparent and lacked vascularityor necrosis. Smears showed cells in short, sometimes anastomosing cords, but alsoas single cells and nondescript cell clusters. Cells displayed a monotonous uniformity in nuclear diameter and cell size. Bland nuclei with evenly dispersed chromatindisplayed variably sized nucleoli, and a moderate amount of infrequently vacuolated cytoplasm. Tissue fragments of variable size were found in 5 of 5 FNA cellblocks. Fluorescence in situ hybridization (FISH) analysis using the EWSR1 probeshowed a positive 22q12 translocation in 2 of 3 FNA cases that were tested. Onecase with negative FISH results on the cytologic preparation showed a positivetranslocation using the same technique in the subsequent resection specimen.CONCLUSIONS. A confident cytologic diagnosis of EMC depends on the presence ofa uniform, round to oval cell population often arranged in cords and set in an abundant myxoid/chondromyxoid background and arising in the appropriate clinicalcontext. If positive, FISH testing (of paraffin cell blocks or cytospin preparations) isconfirmatory when coupled with this cytomorphology. [ABSTRACT FROM AUTHOR]