1. Circulating oxidized LDL, increased in patients with acute myocardial infarction, is accompanied by heavily modified HDL[S]
- Author
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Naoko Sawada, Toshihiro Aiuchi, Yuji Hamazaki, Rina Kato, Masaki Kikuchi, Shinji Koba, Hiroyuki Itabe, Sanju Iwamoto, Takashi Takaki, and Takashi Obama
- Subjects
0301 basic medicine ,medicine.medical_specialty ,Apolipoprotein B ,oxidized high density lipoprotein ,medicine.drug_class ,Lysine ,Myocardial Infarction ,QD415-436 ,030204 cardiovascular system & hematology ,Monoclonal antibody ,Biochemistry ,03 medical and health sciences ,chemistry.chemical_compound ,0302 clinical medicine ,Endocrinology ,High-density lipoprotein ,proteomics ,Internal medicine ,Lipidomics ,medicine ,Humans ,Myocardial infarction ,Research Articles ,biology ,Acrolein ,Cell Biology ,Middle Aged ,medicine.disease ,Lipoproteins, LDL ,Blot ,030104 developmental biology ,chemistry ,Acute Disease ,biology.protein ,lipidomics ,lipids (amino acids, peptides, and proteins) ,oxidized low density lipoprotein ,atherosclerosis ,Lipoproteins, HDL ,apolipoproteins - Abstract
Oxidized LDL (oxLDL) is a known risk factor for atherogenesis. This study aimed to reveal structural features of oxLDL present in human circulation related to atherosclerosis. When LDL was fractionated on an anion-exchange column, in vivo-oxLDL, detected by the anti-oxidized PC (oxPC) mAb, was recovered in flow-through and electronegative LDL [LDL(-)] fractions. The amount of the electronegative in vivo-oxLDL, namely oxLDL in the LDL(-) fraction, present in patients with acute MI was 3-fold higher than that observed in healthy subjects. Surprisingly, the LDL(-) fraction contained apoA1 in addition to apoB, and HDL-sized particles were observed with transmission electron microscopy. In LDL(-) fractions, acrolein adducts were identified at all lysine residues in apoA1, with only a small number of acrolein-modified residues identified in apoB. The amount of oxPC adducts of apoB was higher in the LDL(-) than in the L1 fraction, as determined using Western blotting. The electronegative in vivo-oxLDL was immunologically purified from the LDL(-) fraction with an anti-oxPC mAb. The majority of PC species were not oxidized, whereas oxPC and lysoPC did not accumulate. Here, we propose that there are two types of in vivo-oxLDL in human circulating plasma and the electronegative in vivo-oxLDL accompanies oxidized HDL.
- Published
- 2020