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Increase in Intracellular Ca2+ Concentrations and the Corresponding Intracellular Acidification Are Early Steps for Induction of Apoptosis by Geranylgeraniol in HL60 Cells
- Source :
- Biological and Pharmaceutical Bulletin. 30:880-884
- Publication Year :
- 2007
- Publisher :
- Pharmaceutical Society of Japan, 2007.
-
Abstract
- To elucidate the mechanism of induction of apoptosis by geranylgeraniol (GGO), which is a potent inducer of apoptosis in various lines of human cancer cells, we examined the role of intracellular acidification during GGO-induced apoptosis using human leukemia HL60 cells. Flow cytometry analysis revealed that apoptosis induced in human leukemia HL60 cells by GGO was associated with intracellular acidification. Both GGO-induced intracellular acidification and apoptosis as analyzed by DNA fragmentation were inhibited by phorbol myristate acetate (TPA) and O'-bis(2-aminophenyl)ethyleneglycol-N,N,N',N-tetraacetic acid tetraacetoxymethyl ester (BAPTA-AM), an intracellular Ca(2+) chelator, but not by ethyleneglycol-bis(2-aminoethylether)-N,N,N',N'-tetraacetic acid (EGTA). These results suggest that the early concentration change of intracellular Ca(2+) and the corresponding decrease in intracellular pH are required for the induction of apoptosis in HL60 cells by GGO.
- Subjects :
- HL60
Intracellular pH
Pharmaceutical Science
Apoptosis
HL-60 Cells
DNA Fragmentation
Flow cytometry
chemistry.chemical_compound
Geranylgeraniol
medicine
Humans
Egtazic Acid
Pharmacology
medicine.diagnostic_test
Cytochromes c
General Medicine
Hydrogen-Ion Concentration
Mitochondria
Cell biology
EGTA
chemistry
Tetradecanoylphorbol Acetate
DNA fragmentation
Calcium
Diterpenes
Intracellular
Subjects
Details
- ISSN :
- 13475215 and 09186158
- Volume :
- 30
- Database :
- OpenAIRE
- Journal :
- Biological and Pharmaceutical Bulletin
- Accession number :
- edsair.doi.dedup.....571350480a5bfef27573e2f9694e4903