1. Deletion of the budBAC operon in Klebsiella pneumoniae to understand the physiological role of 2,3-butanediol biosynthesis.
- Author
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Jeong, Daun, Yang, Jeongmo, Lee, Soojin, Kim, Borim, Um, Youngsoon, Kim, Youngrok, Ha, Kyoung-Su, and Lee, Jinwon
- Subjects
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BUTANEDIOL , *BACTERIAL operons , *KLEBSIELLA pneumoniae , *OXIDATION-reduction reaction kinetics , *BIOSYNTHESIS , *PHYSIOLOGY - Abstract
Klebsiella pneumoniaeis known to produce 2,3-butanediol (2,3-BDO), a valuable chemical. InK. pneumoniae, the 2,3-BDO operon (budBAC) is involved in the production of 2,3-BDO. To observe the physiological role of the 2,3-BDO operon in a mixed acid fermentation, we constructed abudBAC-deleted strain (SGSB109). The production of extracellular metabolites, CO2emission, carbon distribution, and NADH/NAD+balance of SGSB109 were compared with the parent strain (SGSB100). When comparing the carbon distribution at 15 hr, four significant differences were observed: in 2,3-BDO biosynthesis, lactate and acetate production (lactate and acetate production increased 2.3-fold and 4.1-fold in SGSB109 compared to SGSB100), CO2emission (higher in SGSB100), and carbon substrate uptake (higher in SGSB100). Previous studies on the inactivation of the 2,3-BDO operon were focused on the increase of 1,3-propanediol production. Few studies have been done observing the role of 2,3-BDO biosynthesis. This study provides a prime insight into the role of 2,3-BDO biosynthesis ofK. pneumoniae. [ABSTRACT FROM AUTHOR]
- Published
- 2016
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