Observation of 2,3-butanediol biosynthesis in Lys regulator mutated Klebsiella pneumoniae at gene transcription level.

Highlights: [•] The effect of budR on 2,3-BDO synthesis was observed at gene transcription level. [•] By λ Red recombination and pUC18 method Klebsiella mutants were constructed. [•] The regulation mechanism for 2,3-BDO synthesis was observed in Klebsiella mutants. [•] 2,3-BDO synthesis was activated by the inducer, regulator, and carbon flux. [•] Results were compared to previously reported results of 2,3-BDO synthesis. [ABSTRACT FROM AUTHOR]