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Gastric cancer is one of the most common cancers globally and has a poor prognosis. MiR‐936 has been reported to regulate cell activity and tumor progression in non‐small cell lung cancer, glioma, and epithelial ovarian cancer. However, the specific role and mechanism of miR‐936 in gastric cancer have not been explored. In present study, gastric cancer cells were transfected with miR‐936 mimic, and cell proliferation, cell cycle distribution, cell apoptosis, migration and invasion were assessed via cell‐counting kit‐8, flow cytometry, wound healing, and transwell assay, respectively. Dual luciferase reporter assay was used to check miR‐936 binding to its downstream target. It was shown that miR‐936 was downregulated in gastric cancer tissues and cells. Erb‐B2 Receptor Tyrosine Kinase 4 (ERBB4) was confirmed as a direct target of miR‐936 and negatively regulated its expression by miR‐936. Overexpression of miR‐936 suppressed cell proliferation, cell cycle progression, cell migration and invasion, and enhanced cell apoptosis in gastric cancer cells, which could be reversed by further ERBB4 overexpression. Western blot results showed that miR‐936/ERBB4 axis regulated Akt‐related pathways to control gastric cancer cell activities. Therefore, our data suggest that miR‐936 overexpression inhibits cell proliferation and invasion and promotes cell apoptosis through Akt‐related pathways by targeting ERBB4, which provides novel insight to target miR‐936 or miR‐936/ERBB4 axis for the treatment of gastric cancer.

The duration of construction project is of great uncertainty because the construction project is of large scale, its construction cycle is long, it takes much consumption in material resources, human and financial resources, it is of complicated cases, and there are also many unpredictable factors. A method to determine the uncertainty duration whose chief path is not a single one is raised combining with engineering practices in this thesis.

Numerical simulation of the die for cylinder upsetting is done by Marc AutoForge software on base of elastic-plastic theoretical mechanical model to analyze transient temperature field, equivalent total strain field and thermal strain field. Then qualitative and quantitative relationship is found between these Factors and die-damage. A new method-Heat Exchange Balance Method is put forward to solve the cooling problems of hot -forging die by combinating with production practice , research practice and using basic theory of finite element and thermal.

In consideration of the crosstalk between reconstructed image and zero-order image existed in the digital reconstruction for off-axis digital holography,this paper presents a method to suppress the zero-order image.Based on the principle that the pixel gray-scale of the image is decided both by the image illumination and surface reflectance,the hologram is considered as a product of the incident and reflected components.The incident components which change slowly are mainly concentrated in the low-frequency region,and the reflected components which determine the detail of the image are concentrated in the high-frequency region.A reasonable homomorphic filter with the upper and lower limits of 0.001 and 1 respectively and a diameter of 300 pixel is designed and processed into the hologram.The results show that the zero-order image can be suppressed with only one Fresnel digital hologram at a distance of 34 cm.Meanwhile,the contrast and the details of reconstructed image at the same distance are improved.

In this paper, theoretical and experimental researches were done to find the repeated cracking reason of stuffing box in a super hyper pressure compressor. The mathematical expression of the assembly pressure was deduced in interference fit. From macro and micro perspectives, residual stresses were measured by X-ray diffraction in typical position of stuffing box when the packing box in combination and separation states. Besides, metallography and fracture examinations were carried out. The results showed that the real magnitude of shrink range of the failed stuffing box was only account for 12% of the designed size requirements, however, under this working condition this stress was not sufficient to cause cracking; Unqualified smelting process and tensile stress concentration in tank caused micro crack, which finally results in fracture of the stuffing box under fatigue mode.

A novel method for the acquisition of full-color three dimensional(3-D) images of real objects was proposed based on multiple projections.In order to improve sampling efficiency,an orthogonal scanning mode was presented,instead of conventional 2-D scanning mode,to capture a series of projection images by a color charge coupled device in both vertically and horizontally direction with an incoherent light source.The 3-D Fourier spectra were extracted by using the projection images.The computer-generated hologram(CGH) was synthesized and the numerical 3-D image was reconstructed.The experimental results show that the acquisition method of multiple projection view images has good performance,high efficiency.Moreover,a new extraction method was adopted to improve viewing quality of 3-D imaging by using the multi-circle sampling area.Comparison with experimental result shows that more available data is used to synthesize the CGH and a significant improvement in image definition.

Background: We have investigated how recirculating lymphocytes patrol the liver in a normal steady state. Methods: Thoracic duct lymphocytes of congeneic rats were intravenously transferred to host rats and donor cell trafficking in the liver and hepatic lymph was examined. Host hepatic lymph nodes (HLNs) were selectively removed, which allowed liver-derived donor cells to collect in the thoracic duct without transit in the intervening HLNs. Results: The number of donor cells in the thoracic duct lymph significantly increased over the baseline 3, 5 and 11 h after transfer in the HLN-removed, non-pretreated, and HLN-ligated (in which a lymph efflux was blocked) groups, respectively. Histologically, donor cells appeared in the portal area from 0.5 h after transfer and frequently attached to the basal lamina of portal vein both externally and internally. Three hours after transfer, a few donor cells appeared in the subcapsular sinus of HLNs. Conclusion: The minimal transit time of rat recirculating lymphocytes is 3–4 h in the liver and 5–8 h in the hepatic LNs, in a normal steady state. Recirculating lymphocytes might transmigrate through the portal vein as well as the sinusoid in the periportal zone. This rapid transit might enable an efficient surveillance of the liver portal area by the recirculating lymphocytes.